The periodontal regeneration is based on the proliferation and mult-differentiation of periodontal ligament cells.Thus,to find a method of enhancing the differentiation of periodontal ligament cells has been a hot point in studying periodontal disease.Many bioactive factors have important effects on the periodontal regeneration. Growth factors as bioactive factors have promotion effects on migration,growth,proliferation,differentiation,and protein synthesis.

Objective To explore the change of serum concentrations of apolipoprotein A5(APOA5),and the relationship with levels of adiponectin and fasting insulin(FINS)in patients with coronary heart disease(CHD).Methods Blood lipids,the serum concentrations of APOA5,adiponectin and insulin were examined in 95 individuals,who were divided into two groups:51 patients with CHD and 44 healthy individuals.Results The concentrations of APOA5 and adiponectin in CHD patients were significantly lower than the healthy individuals[(230.06?115.8)?g/L and(3.03?1.85)?g/L vs(324.43?151.79)?g/L and(4.12?2.48)?g/L respectively,all P

Objective to investigate the characteristics of externalizing problems behavior and related factors in new generation migrant workers so as to provide a decision making basis for consensus propaganda,psychological assistance and intervention.Methods A total of 1 340 new generation migrant workers(male 862,femal 478) were extracted from the gathering areas of mi grant workers in the three cities of Chongqing,Shenzhen and Hangzhou,the questionnaire survey and evaluation were performed by adopting the Youth Self-Report (YSR),Harter Self-Perception Profile(HSPP),Lum Emotional Availability of Parents (LEAP),Perceived Availability of Social Support Questionnaire(PASSQ) and Psychological Acculturation Scale(PAS).Results The tested externalizing problems were slightly higher than the non-clinical tested reasonable scoring range,the peer support had statistical difference between sexes(P＜ 0.01),the personal ability and comprehensive self value had significantly positive correlation (P ＜ 0.01),and both were negatively correlated to the externalizing behavior problems(P＜0.01),parents' emotional availability and support showed significantly negative correlation to externalizing behavior problems(P＜0.01),the working environment support was negatively related to externalizing behavior problems(P＜0.05).The prediction of personal effectiveness to externalizing prob lems behavior was obvious(P＜0.01),the social acceptance(P＜0.01) and overall self value(P＜0.01)were the predictive factors of externalizing problems behavior,and the work environmental support had the predictive effect on externalizing problems behavior (P＜0.05).Conclusion Personal ability,parents' emotional availability and support,work environmental support and social acceptance are correlated with externalizing behavior problems in new generation migrant workers.The overall self value,social accept ance,mother's emotional availability and parents' emotional support have significant prediction,while the work environmental support has a predictive effect on externalizing behavior problems.

Objective To explore the effect of swimming and caressing on neonatal jaundice and weight.Methods Two hundred and forty newborns were divided equally into control and observation group with random digits table.The former received care with caressing and the latter with swimming and caressing.The two groups were compared in terms of weights and jaundice indices at birth and 7d after birth.Results Seven days after birth,the jaundice index in the observation group were lower than that of the control group and the weights of the observation group were larger(P<0.05).Conclusion Swimming and caressing are helpful for the development of neonates by C-sect and the reduction of jaundice duration.

Objective To study the association of early prognosis and risk factors about early neurological deterioration (END) in aged patients with cerebral hemorrhage in basal ganglia. Methods One hundred and thirty-nine aged patients with cerebral hemorrhage in basal ganglia were selected. The patients were divided into END group (59 cases) and non-END group (80 cases), the clinical data were retrospectively analyzed. The patients were followed up 3 months after discharge, the prognosis was evaluated by modified Rankin scale. Logistic regression analysis was used to determine the independent risk factors of END. Results The incidence of END was 42.4%(59/139). The incidence of unfavourable prognosis in END group was significantly higher than that in non-END group:78.0%(46/59) vs. 30.0%(24/80), and there was statistical difference (P<0.05). The score of Canadian stroke scale (CSS) at admission in END group was significantly lower than that in non-END group: (5.2 ± 2.1) scores vs. (6.9 ± 1.7) scores, but the white blood cell count at admission, hematoma volume and brain ventricle hemorrhage rate were significantly higher than those in non-END group: (10.7 ± 2.9) × 109/L vs. (7.9 ± 2.4) × 109/L, (17.4 ± 14.9) ml vs. (11.2 ± 10.5) ml and 42.4%(25/59) vs. 18.8%(15/80), and there were statistical differences (P < 0.05). There were no statistical differences in sex, age, systolic blood pressure, diastolic blood pressure, body temperature, random blood glucose, fibrinogen, activated partial thromboplastin time, prothrombin time and the incidences of hypertension, smoking history, stroke, diabetes mellitus, hyperlipidemia between 2 groups (P>0.05). The Logistic regression analysis results showed that brain ventricle hemorrhage and white blood cell count at admission were independent risks factor for END in aged patients with cerebral hemorrhage in basal ganglia(P < 0.05 or < 0.01). Conclusions The aged patients with cerebral hemorrhage in basal ganglia will be prone to END, the brain ventricle hemorrhage and white blood cell count at admission are independent risks factor for END, and the early prognosis is poor.

Objective To explore the cost of healthcare-associated infection (HAI)management in a tertiary first-class hospital,provide data support for cost-effectiveness and cost-benefit analysis of HAI management,and provide scientific evidence for the rational allocation of hospital resources.Methods Micro-costing study was used to calcu-late the direct cost of the department of HAI management by collecting the quantity and unit price of each item. Results The total cost of HAI management in this hospital in 2013 were about ￥870 000,including human cost￥790 000,depreciated fixed assets ￥34 501 ,low-value consumption goods ￥3 800,publicity and training￥33 600,office consumables ￥5 208;average cost were ￥12.16 per person and ￥529.69 per bed.Conclusion Human cost is the main cost in HAI management in this hospital.

Objective: Design a test phantom with a full function, simple structure and high accuracy which is used for MRI’s Quality Control. Methods: Through a intensive study in imaging principle of MRI and parameters which effect the image quality ,first come up with the phantom’s performance data, and then determine the technical solutions including the choosing material, processing technology, the machining precision, at last develop a trial set of MRI test phantom using the method of numerical control machining. Make a comparative experiment between this phantom and the similar imported one to analyze the consistency of the results by the two phantoms. Results: The result of all the technical parameters of this phantom is consistent with the result of the imported one. There are some extra test components which the imported phantom doesn’t contain. Some performance data like the low contrast resolution are superior to the imported phantom. Conclusion:This MRI test phantom can satisfy the requirement of the daily quality control work in hospital.

Age-related white matter changes are considered as a manifestation of arteriolosclerotic small vessel disease and are associated with advanced age and vascular risk factors.White matter changes have been recognized as one of the manifestations of cerebral small vessel disease.They are the pathological basis of cognitive impairment and functional loss in the elderly.Studies have shown that when white matter changes develop to a certain extent,there will be many clinical symptoms,including cognitive impairment,dementia,depression,gait disturbances,and urinary incontinence,and they are associated with the increased risks of stroke and death.

Objective To evaluate the roles of B-type natriuretic peptide (BNP) in predicting the severity of community-acquired pneumonia (CAP) by studying the correlation between them.Methods A total of 202 patients with CAP admitted from December 2011 to December 2012 were enrolled in this study.All these patients were checked with laboratory tests for BNP level,C-reactive protein (CRP),white blood cell count (WBC) as well as other markers needed for obtaining pneumonia severity index (PSI).The differences in BNP levels,CRP levels,and WBC were compared among different degrees of pneumonia severity,and the correlation between BNP levels and PSI was investigated by a linear correlation analysis.The patients enrolled were divided into a high-risk group (defined as Ⅳ-Ⅴ grade of PSI) and a low-risk group (defined as Ⅰ-Ⅲgrade of PSI).Meanwhile,they were also divided into a survivor group and a non-survivor group according to outcomes.BNP levels between the two groups were compared,and a receiver operating characteristic (ROC) curve analysis was performed on the BNP levels versus PSI.Results BNP levels increased with CAP severity (r =0.782,P ＜0.01).The mean level of BNP (263.2 ± 119.6) pg/mLof patients in the high-risk group was significantly higher than that of patients (71.5 ± 54.3) pg/mL in the low-risk group (P ＜ 0.01).The patients in the non-survivor group had significantly higher BNP levels compared to the survivor group [(343.86 ± 125.49) vs.(183.00 ±121.71) pg/mL,P ＜ 0.01].In addition,there were positive correlations between BNP levels and PSI (r =0.782,P＜0.001),between BNP levels and CRP levels (r =0.560,P＜0.01),and between BNP levels and WBC (r =0.513,P＜0.001).The BNP level had a high accuracy in predicting the severity of CAP (AUC =0.952).The optimal cut-off point of BNP level for distinguishing high-risk from low-risk patients was 125.0 pg/mL,with a semitivity of 0.891 and a specificity of 0.946.Moreover,BNP level had a definite accuracy in predicting mortality (AUC =0.823).Its optimal cut-off point for predicting death was 299.0 pg/mL,with a sensitivity of 0.675 and a specificity of 0.816; its negative predictive cut-off value was 0.926,and positive predictive value was 0.426.Conclusions BNP level is positively correlated with the severity of CAP.The patient with BNP level above 125.0 pg/mL should be hospitalized immediately and patients with BNP level higher than 299.0 pg/mL are at the high risk of death.Therefore,BNP is a useful biomarker for evaluating the severity of patients with CAP.

To optimize the solvent system of high-speed counter-current chromatography (HSCCC) for diterpenoidtanshinone separation and define it's antitumor activity in vitro. Total diterpenoidtanshinone was made by CO2 supercritical fluid extraction (SFE), UPLC was applied to determining the peak area of tanshinone IIA and cryptotanshinone in different solvents up or under phase, and the partition coefficient K values of them were calculated. CKK-8 was used to observe the inhibitory effects of diterpenoidtanshinone on human liver cancer (QGY-7703), lung cancer (PC9, A549), gastric cancer (MKN-45, HGC-27), colon cancer (HCT116), myeloma (U266, RPMI8226), and breast cancer (MCF-7). The best solvent system for HSCCC was petroleum ether-ethyl acetate-methanol-aqua (12∶8∶ 13∶7). The yield of diterpenoidtanshinone was 8.65%. Diterpenoidtanshinone has good effect of antitumor in vitro especially on human PC9 cell. The selected solvent system is suitable for diterpenoidtanshinone separation by HSCCC, and the established HSCCC method is reliable and easy for operating. Diterpenoidtanshinone has good antitumor effect in vitro.