China ; Humans ; Periodontics ; trends

Dental Scaling ; Dentistry ; manpower ; General Practice, Dental ; manpower ; Health Education, Dental ; manpower ; Humans ; Oral Hygiene ; Outcome Assessment (Health Care) ; Periodontitis ; therapy ; Toothbrushing

Aggressive Periodontitis ; diagnosis ; therapy ; Humans

Dental Implantation, Endosseous ; Dental Implants ; Dental Restoration Failure ; Humans ; Prognosis

Volatile oil components and the contents and types of amino acid in spica of Prunella vulgaris were analysed by GC-MS and amino acid analyzer. Esters, fatty acids, aromatic hydrocarbon, ketone and several alcohol compounds were identified by mass spectrum comparison. In these ingredients, beta-ionone smelled aroma of cedar, raspberry, nerolidol showed weak sweet soft orange blossom flavor, neroli tasted sweet and fresh, nerolidol tasted sweet with light aroma of wood, hexadecanal showed a weak aroma of flowers and wax, alpha-sinensal had rich and fresh sweet orange flavor. To some extent, these types of aromatic substances can affect the taste of herbal tea or decoction made of Spica Prunellae. Among amino acids detected, natural amino acids accounted for a larger proportion, and those natural amino acids showed bitterness, slight bitterness, sourness (freshness), sweetness, slight sweetness, sourness (slight freshness). The results indicated that bitter and slightly bitter amino acids have the greatest impacts on the sense of Spica Prunellae.
Amino Acids ; analysis ; Gas Chromatography-Mass Spectrometry ; Oils, Volatile ; analysis ; Prunella ; chemistry ; Taste

OBJECTIVETo observe root surfaces and analyze the mineral contents in cementum of patients with rapidly progressive periodontitis (RPP).

METHODSTen teeth were selected from RPP patients, adult periodontitis (AP) patients and healthy (H) control subjects respectively, and prepared for scanning electron microscopy (JSM-35C). The energy-dispersive X-ray analysis was used to measure the mineral contents in cementum (Ca, P, Mg).

RESULTSThe root surfaces of RPP teeth were similar to that of AP teeth. Ca: 64.60% +/- 2.29% vs 63.54% +/- 2.97%, P > 0.05; P: 32.54% +/- 1.29% vs 32.97% +/- 2.40%, P > 0.05; Mg: 2.86% +/- 1.29% vs 3.48% +/- 1.02%, P > 0.05; Ca/P: 2.00 +/- 0.17 vs 1.95 +/- 0.25, P > 0.05. There were no differences in Ca, P, Mg contents and Ca/P ratio between RPP teeth and AP teeth.

CONCLUSIONThere may not be cementoplasia in a part of RPP teeth.

Adult ; Dental Cementum ; chemistry ; ultrastructure ; Humans ; Microscopy, Electron, Scanning ; Periodontitis ; pathology ; Tooth Root ; chemistry ; ultrastructure

OBJECTIVETo investigate the change of secretory leukocyte protease inhibitor (SLPI) and elastase (EA) in the different stages of periodontal inflammation and to evaluate the possibility of the two proteins as saliva markers reflecting overall periodontal health status.

METHODSUnstimulated whole saliva were collected from 86 subjects (divided into 4 groups as healthy, gingivitis, moderate periodontitis and severe periodontitis). Fifteen patients with moderate or severe periodontitis were only given scaling and root planning (SRP). Whole saliva was collected and clinical patameters were recorded at baseline and four weeks after the treatment. SLPI concentrations were determined with enzyme linked immunosorbent assay (ELISA) systems, while EA with low-molecular-weight substrate reaction.

RESULTSThere were no statistical differences of SLPI concentrations among four groups (P > 0.05). However, EA activities in moderate periodontitis and severe periodontitis groups [0.077 (0.060) and 0.077 (0.489)] were higher than in healthy and gingivitis group [0.058 (0.028) and 0.058 (0.024)] (P < 0.05). SLPI only showed a weak negative correlation with age (r = -0.301, P < 0.05), rather than with EA or clinical parameters. In 15 patients with chronic periodontitis the mean concentration of SLPI and EA activity was 2.031 (2.449) µg/L and 0.075 (0.118), and both decreased significantly to 1.405 (0.659) µg/L and 0.055 (0.028) respectively 4 weeks after SRP.

CONCLUSIONSAfter SRP, the decrease of SLPI concentration and EA activity in saliva may reflect the periodontal inflammation subsiding. SLPI in saliva was not correlated with the development of periodontal inflammation.

Adult ; Chronic Periodontitis ; metabolism ; therapy ; Dental Plaque Index ; Dental Scaling ; Female ; Gingivitis ; metabolism ; Humans ; Male ; Middle Aged ; Pancreatic Elastase ; metabolism ; Periodontal Index ; Periodontitis ; metabolism ; therapy ; Root Planing ; Saliva ; metabolism ; Secretory Leukocyte Peptidase Inhibitor ; metabolism ; Young Adult

OBJECTIVETo investigate human cytomegalovirus (HCMV) and Epstein-Barr virus-type 1 (EBV-1) in GCF and saliva during experimental gingivitis in Chinese young subjects and to evaluate the effect of the virus in the initial stage of gingival inflammation.

METHODSGCF of 14 and 45 and saliva without stimulating in 11 Chinese young males with healthy gingiva were collected at baseline (day 0), day 7, 14 and 21 after stopping oral hygiene and day7 after reestablishing oral hygiene (day 28). DNA of HCMV and EBV-1 were detected by nested-polymerase chain reaction (n-PCR) at the times mentioned above.

RESULTSHCMV was detected in GCF of 4 subjects at baseline, 4 subjects at day 7, 3 subjects at day 14 and 2 subjects at day 21 while the subjects were different. At day 28 HCMV could not be detected. EBV-1 was not detectable in GCF during the experimental gingivitis. HCMV was detected in saliva in 4 subjects and EBV-1 was in 3 subjects. And there is no relationship between the detection of the herpesviruses and the clinical parameters as well.

CONCLUSIONWe suggest that HCMV and EBV-1 are not the important factors during the initial stage of gingival inflammation.

Adult ; Cytomegalovirus ; isolation & purification ; Gingival Crevicular Fluid ; virology ; Gingivitis ; virology ; Herpesvirus 4, Human ; isolation & purification ; Humans ; Male ; Young Adult

Acute Disease ; Animals ; Behavior, Animal ; drug effects ; Female ; Male ; Mice ; Mice, Inbred ICR ; Nitriles ; poisoning ; Rabbits

OBJECTIVETo test the bovine cementoblasts (CBs) cementum-forming ability in vivo.

METHODSRoot fragments of newborn bovine freshly extracted mandibular incisor were cultured routinely and 4th-5th passages of CBs were harvested. CBs were then cultured in the medium supplemented with 50 mg/L alpha-ascorbic acid and 10 mmol/l beta-glycerolphosphate to form a thick layer as tissue engineering scaffold for cementum formation. Collagen membrane was used as control scaffold. 2 x 10(6) cells were attached to the CBs-made carrier as well as collagen membrane scaffolds and transplanted subcutaneously into immunodeficient mice. Transplants were harvested at 7th week. Histological sections were stained with HE, alizarin red S and van Kossa methods as well as monoclonal Ab against bovine cementum attachment protein (CAP).

RESULTSCBs-made scaffold supported more cementum-like tissue (CLT) formation than collagen-made scaffold. The CLT formed on CBs scaffold was partly calcified with embedded cells. Uncalcified cementoid-like material could be seen on the surface and was encircled by cubical CB-like cells. The CLT was also positive to CAP and van Kossa staining.

CONCLUSIONSThese results suggest that the bovine CBs can form cementum-like tissue. The cell-made carrier is a better scaffold than collagen membrane.

Alkaline Phosphatase ; analysis ; Animals ; Bone Transplantation ; methods ; Cattle ; Cell Adhesion Molecules ; analysis ; Cells, Cultured ; Dental Cementum ; chemistry ; cytology ; transplantation ; Immunohistochemistry ; Integrin-Binding Sialoprotein ; Male ; Mice ; Mice, Nude ; Osteocalcin ; analysis ; Osteonectin ; analysis ; Sialoglycoproteins ; analysis ; Tissue Engineering ; methods ; Transplantation, Heterologous