1.Research progress of TKTL1 in malignant neoplasms
Cancer Research and Clinic 2015;27(5):352-355
Up to now,one transketolase (TKT) and two transketolase-like genes (TKTL1 and TKTL2) have been identified in the human genome.People have conducted a series of basic and clinical researches of TKTL1 in malignant neoplasms since Coy found TKTL1 has a high expression of protein and gene levels in the malignant tumors in 2005.Although the biochemical and functional mechanism have not been clarified clearly,a positive role of TKTL1 in promoting tumorigenesis and tumor progression has been proved.
2.Content Changes of Aristolochic Acid-A from Caulis Aristolochiae Manshuriensis and Its Processed Products
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(09):-
Objective To research the content changes of Aristolochic Acid-A from Caulis Aristolochiae Manshuriensis and its processed products.Method Chromatographic assay was performed on Lichrospher-C18 column (4.6 mm?200 mm,5 ?m) with methanol-0.1% glacial acetic acid solution (70∶30) as mobile phase,the flow rate was 1.0 mL/min and the detection wavelength was set at 310 nm,the column temperature was 30 ℃.Result The content of Aristolochic Acid-A was lower in three processed products than in crude drugs,and the reduction rate of the one which was boiled by NaHCO3 was the highest.Conclusion The three processed method can reduce the content of Aristolochic Acid-A,and achieve the aim of reducing the toxicity.
3.Study on the Quality Standard for Qingzao Runfei Mixture
Juanjuan ZHOU ; Jinhuo PAN ; Huan LU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(08):-
Objective To establish quality control method for Qingzao Runfei Mixture. Methods Mulberry Leaves,Glycyrrhiza,Radix Glehniae,Ophiopogon Japonicus and Loquat Leaves were identified by TLC. Glycyrrhizic Acid was determinated by HPLC. Results The negative sample of TLC had no interference. The specifity was good. Glycyrrhizic acid showed a good linear relationship in the range of 0.203 1~1.692 1 ?g,r =0.999 8. The average recovery was 98.24%,and RSD was 2.3%. Conclusion The established methods are simple,quick and with good reproducibility. This study provides methods for the quality control of Qingzao Runfei Mixture.
4.Effects of Huanglian Jiedu Decoction on phosphatidylinositol-3-kinase expression in target tissues of type 2 diabetic rats
Dan JIN ; Fuer LU ; Guang CHEN ; Huan SUN ; Xiaohong LU
Journal of Integrative Medicine 2007;5(5):541-5
OBJECTIVE: To observe the effects of Huanglian Jiedu Decoction (HLJDD), a traditional Chinese compound herbal medicine, on p85 mRNA and protein expressions of phosphatidylinositol-3-kinase (PI-3K) in target tissues (skeletal muscular and adipose tissues) in rats with type 2 diabetes mellitus (T2DM) and to investigate the molecular mechanism of HLJDD in treating T2DM. METHODS: The male Wistar rats were injected with streptozotocin (STZ) 30 mg/kg through tail vein, and fed with high-fat and high-caloric diets to induce T2DM. Then the rats were randomly divided into untreated group, aspirin-treated group and HLJDD group, and treated correspondingly. Meanwhile, a group of normal animals without any treatment was set up for normal control group. Ten weeks later, serum fasting blood glucose (FBG), serum fasting insulin (FINS) and oral glucose tolerance test (OGTT) were routinely determined. The expressions of PI-3K p85 mRNA and protein in skeletal muscle and adipose tissue were determined with RT-PCR and Western blotting before and after insulin treatment. RESULTS: Compared with the untreated group, the FBG and OGTT levels in T2DM rats treated with HLJDD decreased significantly (P<0.05). The FINS in HLJDD group was lower than that in the normal control group (P<0.05), but was not significantly different from that in the untreated group. The PI-3K p85 mRNA and protein expressions in HLJDD group obviously increased, as compared with those in the untreated group. CONCLUSION: The effect of HLJDD in treating T2DM was probably associated with its improvement of PI-3K p85 mRNA and protein expressions in skeletal muscle and adipose tissue of the T2DM rats.
6.Research Progress of Event-related Potential in Mild Cognitive Impairment.
Meng-meng JI ; Huan-huan MENG ; Bin LUO ; Xi-ping CHEN ; Lu-yang TAO
Journal of Forensic Medicine 2015;31(3):215-218
Mild cognitive impairment caused by craniocerebral trauma is the key points and difficulties in judicial authentication. This article has comparative analysis of each mode of event-related potential (classical Oddball, Eriksen flanker task and so on), which can provide a more objective method for such craniocerebral trauma cases in clinical forensic judicial authentication.
Cognitive Dysfunction
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Craniocerebral Trauma
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Evoked Potentials
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Forensic Sciences
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Humans
8.The selection of irradiation volume for neck lymph node-negative nasopharyngeal carcinoma: the results based on a Meta-analysis study
Jiale HUAN ; Pengfei XING ; Yingying XU ; Hongxia WANG ; Xueguan LU
Chinese Journal of Radiological Medicine and Protection 2014;34(4):275-278
Objective To study the appropriate neck irradiation volume for neck lymph nodenegative nasopharyngeal carcinoma patients by Meta-analysis.Methods The related references published from Jan 1990 to Jun 2013 were searched in Pubmed,Embase,Cochrane and Wangfang Databases.The qualified references for enrollment criteria were screened and the relative data were collected.The OR (Odds ratio) value by fixed effect model was used to evaluate the data for patients who received radiotherapy between the upper neck and whole neck irradiation.The endpoints including 5-year neck local control rate (LCR),neck-in-irradiation LCR,and neck-outsides-irradiation LCR.Results Five qualified references were screened and belonged to respective research.There enrolled 1 333 patients,including 970 patients who received the upper neck irradiation and 363 patients who received the whole neck irradiation,respectively.The forest plots revealed that there were no significant differences for 5-year neck LCR,neckin-irradiation LCR,and neck-outsides-irradiation LCR for patients received radiotherapy between the upper neck and whole neck irradiation.These OR values were 0.89 (95% CI:0.41-1.94),1.29 (95% CI:0.58-2.88) and 0.42 (95% CI:0.07-2.36),respectively.Conclusions The Meta-analysis results suggest that irradiation to the upper neck for neck lymph node-negative nasopharyngeal carcinoma can be appropriate.
9.Distribution of drug resistance Acinetobacter Baumannii in surgical ICU and its prevention
Bei WANG ; Huan YANG ; Chen LU ; Hongyi YI
Chinese Journal of General Practitioners 2011;10(7):495-496
Drug resistance of Acinetobacter baumannii ( Ab) in surgical ICU from January 2008 to December 2009 was investigated retrospectively. Total 114 clinical strains of Ab were isolated from surgical ICU and 92 strains were from respiratory tract (80.7% ). The prevalence rate of multiresistant Ab in 2009 was higher than that in 2008 (75. 7% vs 50. 0% , x2 = 7. 703, P = 0. 006). The results indicate that to monitor and control drug resistant of Ab constantly is important for the prevention of nosocomial infection.
10.Clinical analysis of 233 patients with drug eruption
Yixuan WU ; Huan FENG ; Yiwen LIU ; Chengfeng QIAN ; Ping LU
Chinese Journal of New Drugs and Clinical Remedies 2001;20(2):133-134
AIM:To discuss the main clinical characteristics of current drug eruption for reference of prevention and treatment of drug eruption. METHODS: Two hundred and thirty-three with drug eruption in 1999 were collected, and their clinical characteristics, such as age, main causative drugs and eruption types, etc. were analysed. RESULTS: The age of current drug eruption increased. Antibiotics was the most common causative drug, while antiinflammatory analgestics, serum and vaccine, traditional Chinese medicine were next to it. Scarlet fever-like or measles-like type was the most common eruption type, while urticaria type, erythema multiforme type and fixed type were next to it. CONCLUSION: Clinical physicians shoud inquire alergic history of patients thoroughly and avoid unnecessary drugs, so as to decrease the occurrence of drug eruption.