1.Advance research on space analysis related factors
Xianyue ZHANG ; Huan JIANG ; Min HU
Journal of Medical Postgraduates 2014;(7):763-766
Orthodontics is the procedure of managing the space .Space analysis is a comprehensive quantitative analysis that can analyze the factors that influence the arch space .It also helps to judge if the treatment goals can be reached , thus helping the treat-ment plan making and guiding clinical orthodontics .And space analysis related factors include crowding , curve of Spee, arch width, arch growth, arch protrusion, mesiodistal angulation, an so on.In this review, we summarizes the above-mentioned factors.
2.Advance research on protein tyrosine phosphatase SHP-2 and the regulating role of it in osteoclastogenesis
Peng LIN ; Huan JIANG ; Min HU
Journal of Medical Postgraduates 2014;(9):990-993
SHP-2 is one of the protein tyrosine phosphatases which plays a role in the progress of dephosphorylation in organ -isms, participating in many kinds of signal transduction pathways .The mutation of SHP-2 is associated with many kinds of malignant diseases .In recent years , scholars have found that SHP-2 is the key factor in osteoclastogenesis , playing a positive role in the progress of reversible phosphorylation of osteoclasts .The objective of this article is to review the molecular biological characteristics of SHP-2, th diseases associated with the mutation of SHP-2 and the effects of SHP-2 in regulation of osteoclastogenesis .
3.Prokaryotic expression of streptolysin O antigen and its condition optimization
Xilin HU ; Huan HUA ; Qunying DENG
International Journal of Laboratory Medicine 2014;(19):2581-2583
Objective To construct the recombinant streptolysin O antigen(SLO) prokaryotic expression plasmid and establish its best expression condition in Escherichia coli .Methods The DNA fragment encoding SLO was amplified from streptococcal ge-nomic DNA template by PCR ,and then incorporated into pET-32a(+ ) vector to construct pET-32a(+ )-SLO recombinant plas-mid .pET-32a(+ )-SLO was transformed into Escherichia coli BL21(DE3) and SLO protein was expressed and purified by isopro-pyl-β-D-thiogalactoside(IPTG)-induction and auto-induction ,respectively .Results The results of DNA electrophoresis and DNA sequencing showed that pET-32a(+ )-SLO recombinant plasmid was constructed successfully .When IPTG at different concentra-tion was used ,SLO expressed as inclusion body and its expression efficiency was low .Under auto-induction condition ,SLO ex-pressed as partly soluble manner ,and its expression efficiency increased .Conclusion The prokaryotic expression plasmid pET-32a (+ )-SLO is constructed successfully and the best condition for SLO expression and purification from Escherichia coli culture is es-tablished ,which lay the foundation for further basic and clinical application research with SLO .
4.Prescription parsing of miao medicine Polygonum capitatum and kelintong capsule.
Nan HU ; Shi-Huan TANG ; Long CHENG
China Journal of Chinese Materia Medica 2014;39(7):1318-1320
By literature survey searching references and parsing prescriptions, the auther has analyze the clinical advantage of Miao medicine in the treatment of symptom heat stranguria. Guizhou Miao medicine Polygonum capitatum has many advantages such as resources and clinical. After companying with Phellodendri Cortex, the compound prescription plays the pharmacological activity of antipyretic and diuretic, especially for the symptom heat stranguria, damp and hot junction based in the bladder. Miao medicine Kelintong capsule showed clinical advantage in the treatment of symptom heat stranguria, having a clinical advantage in improving the overall effectiveness and improve the overall aspects of the patient's symptoms.
Animals
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Capsules
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chemistry
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therapeutic use
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Drug Prescriptions
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Drugs, Chinese Herbal
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chemistry
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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Polygonum
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chemistry
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Urination Disorders
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drug therapy
5.Health-Related Quality of Life of Children with Subclinical Epileptiform Electro encephalographic Discharges
ying, HU ; hong-wen, HU ; hai-huan, ZENG
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objectives To study health-related quality of life(HRQOL) of children with subclinical epileptiform electroencephalographic discharges.Methods Subjects were 30 children with subclinical epileptiform electroencephalographic discharges,50 children with clinical seizures,28 children without clinical seizures and 30 controls.Guardians completed a valid epilepsy-specific HRQOL questionnaire for children,the quality of life in children epilepsy questionnaire(QOLCE).Results Children with subclinical epileptiform electroencephalographic discharges have 8 subscales scores lower than control group,2 subscales scores higher than group with clinical seizures.Conclusions Quality of life of children with subclinical epileptiform electroencephalographic discharges is better than that of children with clinical seizures.However cognitive,behavior,daily life and social activity are impaired by subclinical epileptiform electroencephalographic discharges and lowered the HRQOL of children.
6.Maxillary first premolar with 3 root on both sides of a subjet:case report
Bo CHEN ; Huan HU ; Dong LI ; Hu SHI
Journal of Practical Stomatology 2016;32(6):874-875
The maxillary first premolar is a transitional tooth between incisors and molars. Most maxillary first premolars have 2 root canals. However, the bilateral maxillary first premolar with 3 roots and 3 canals has not been described. This article reports a rare case:The bilateral maxillary first premolar with 3 roots and 3 canals.
7.Study of infection of Echinococcus granulosus in yak in spring and its potential role in transmission of cystic Echinococcosis in Rangtang County of Sichuan, China.
Huan Huan HU ; Wei Ping WU ; Li Ying WANG ; Qian WANG ; Yan HUANG ; Ya Yi GUAN
Biomedical and Environmental Sciences 2013;26(3):226-229
Animals
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Cattle
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China
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epidemiology
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Echinococcosis
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epidemiology
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parasitology
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transmission
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Echinococcus granulosus
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pathogenicity
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Humans
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Seasons
9.Current Application Status and Countermeasures of Antibiotics Irrational Use in China
Mengjun HUANG ; Huan XIANG ; Ronggao JIANG ; Chengbo HU ; Binjie LUO
China Pharmacist 2017;20(4):732-735
In recent years,antibiotics have been playing more and more important roles in maintaining human health with the increasing variety and quantity.However,under the influence of different reasons caused by subjective or objective factors,antibiotics irrational use has induced bacterial resistance and adverse reactions,which are becoming more and more serious.The paper put forward the countermeasures of antibiotics irrational use and provided reference for rational use of antibiotics based on the present situation analysis in China and foreign experience in order to control the irrational use.
10.Bacteria Distribution and Antibiotic Resistance Analysis of Urinary Tract Infection in 2004
Huan CHEN ; Yunjian HU ; Yonghui MAO ; Jianshe LIU
Chinese Journal of Nosocomiology 2006;0(03):-
OBJECTIVE To investigate the bacteria distribution and antibiotic resistance in urinary tract infection in 2004.METHODS During Jan 1st 2004 to Dec 31st 2004,1007 urine specimens were collected from inpatient and outpatient departments of Beijing Hospital.Totally 632 strains of pathogens were identified and the drug resistance was(performed.) RESULTS Among pathogens of urinary tract infection in 2004,Escherichia coli rated the first(38.29%),(followed) by Enterococcus(18.67%),fungi(17.41%),Streptococcus(8.07%),Proteus(3.4%),Staphylococcus(3.95%),Pseudomonas aeruginosa(3.17%) and Klebsiella pneumoniae(2.37%).Enterobacteriaceae were sensitive to imipenem(100%).(G~+) cocci were sensitive to nitrofurantoin and vancomycin(nearly to 100%).Compared to pathogens of UTI in 2001,fungi showed obviously increasing trend.CONCLUSIONS In 2004,(Enterobacteriaceae)(mostly E.coli) are the major pathogens in urinary tract infection.Fungi infection in(urinary) tract has an obviously increasing tendency and should be carefully treated.