Objective Insulin resistance is a possible cause of polycystic ovary syndrome(PCOS).It is presumed that ectonucleotide pyrophosphatase/phosphodiesterase 1(ENPP1) is associated with insulin resistance.The purpose of this study is to explore the expression of ENPP1 in granulosa cells of the ovary and its relationship with PCOS. Methods Twelve aliquots of follicular granulosa cells were isolated from 12 samples of the patients with PCOS and 22 aliquots from 22 samples of the patients without PCOS,respectively.ENPP1 expression was analyzed by reverse transcription polymerase chain reaction(RT-PCR) and in situ hybridization.The relative expression of ENPP1 mRNA was detected by real-time quantitative polymerase chain reaction(real-time PCR). Results ENPP1 was expressed in granulosa cells of the ovary.ENPP1 expression's 2~(-?Ct) in granulosa cells of PCOS was significantly higher than non-PCOS(1.67?0.89 vs.0.94?0.76,P=0.017).Conclusion ENPP1 expression plays a role in ovary function and may be closely associated with the development of PCOS.

Initiation of insulin therapy is challenging in the primary care setting without nursing support. Doctors have to prepare their practices to deal with these challenges in order not to delay insulin therapy when needed.

Delivery of Health Care, Integrated ; trends ; Diabetes Mellitus ; epidemiology ; therapy ; Health Promotion ; Health Surveys ; Humans ; Singapore ; epidemiology

Coal Mining ; Humans ; Occupational Diseases ; prevention & control ; Occupational Health ; Tuberculosis ; prevention & control

The serum myocardial enzymes and isoenzymes activities were measured in 65 bum patients. The results showed that serum CK, CK-MB, LDH and AST activities, and CK-MB/CK and LDH1/LDH2 ratios were markedly elevated after burn injury. The CK-MB/CK ratio in major bum patients was higher than 0.05 1 -3d and 14d postbum. The results suggest that myocardial cells of serious bum patients are significantly injured in both early period and 14 d postbum.

Objective To build model of intestinal motility disorders (IMD) in mice after severe craniocerebral trauma by using modified free fall impact method so as to lay the foundation for further study on development mechanism of postinjury IMD.Methods Severe craniocerebral trauma in mice was induced by a cylindrical sleeve rod impactor.Pathological changes of cerebral and intestinal tissue in mice were observed at 1,6 hours and 1,3,7 days after severe craniocerebral trauma.Brain water content and intestinal propulsion percentage were detected in each time point as well.Results Acute inflammation and congestion of the intestine were observed in phase of cerebral hemorrhage and edema after severe craniocerebral trauma in mice.Brain water content was increased at 6 hours and reached the peak at 24 hours.Intestine propulsion percentage showed a fall at one hour and dropped to the lowest level at 6 hours.Conclusions IMD exists in mice with severe craniocerebral trauma.The device involving modified Feeney free fall impact method can successfully establish animal model of IMD in mice with severe craniocerebral trauma.

OBJECTIVE To investigate the drug resistance and distribution of nosocomial infection (NI) pathogens. METHODS A total of 519 strains of NI pathogens during from Jan 2006 to Dec 2007 were completely surveyed. RESULTS Among all pathogens,Gram-positive cocci were 209 strains (40.27%),the main pathogen was Staphylococcus aureus. Gram-negative bacilli were 256 strains (49.33%). Fungi were 54 strains (8.28%). resistance the rate in nosocomial infectionis was increased year by year. CONCLUSIONS The Gram-negatives of NI pathogens are markedly increased year by year. The drug resistance rate of pathogens is higher and the clinically selected anti-bacterial drugs should be based on the bacterial culture and drug susceptibility test.

OBJECTIVE To intensify the management of the acupuncture department and prevent and control nosocomial infection.METHODS During the course of the management of the acupuncture department,the causes of nosocomial infection were found through analyzing the possible reasons of nosocomial infection and a series of relevant measures were taken to improve the control of nosocomial infection.RESULTS In order to control nosocomial infection in acupuncture department we needed to strength the disinfection management in the following aspects:air in the room,medical procedures and implement,medical staff hands and medical disposals.CONCLUSIONS Strict management is the important measures to reduce the nosocomial infection.

Objective To explore the differentiation of rat bone marrow mesenchymal stem cells into dopaminergic neuron.Methods The mesenchymal stem cells were isolated from bone marrow. They were induced to differentiate into dopaminergic neurons by freated with bFGF, VitC and EGF at the third generation. Dopamine-associated protein and genes in the treated cells were examined by immumofluorescence and RT-PCR. Dopamine in the supernatant and endoplasm from culture system was determined by ELISA kit. Results The results showed that tyrosine hydroxylase, dopamine transporter and nerve neucleoprotein and Nestin,Nurr-1 genes were found. And the dopamine existed in the supernatant and cytoplasm from inducing culture system. Conclusion The rat bone marrow mesenchymal stem cells have the capacity of differenting into dopaminergic nurons.

OBJECTIVE To explore the ward-round by hospital director to control nosocomial infection.METHODS To investigate prevalence rate of the nosocomial infection the day before the ward-round by hospital director.At the ward-round day to check the situation of disinfection,isolation,monitoring and control of the nosocomial infection,the hands-washing of the medical workers and the medical waste management.RESULTS To intensity the management of nosocomial infection of the clinical departments,to intensify the ideas of the prevention and control of the nosocomial infection among the medical workers,and to raise their compliance,in order to effectively control the nosocomial infection.CONCLUSIONS Strict and all-round management especially in participation of hospital director is the important measures to control the nosocomial infection.