Objective　To study the toxicity of Tripterygium Hypolaucun (Level) Hutch (THH). Methods　The flow cytometry technique was used to measure the micronucleus (MN) formation and cell apoptosis of Jurkat, CHE and NIH3T3 cell lines induced by THH. Results　THH could induce micronucleus formation and cell apoptosis of Jurkat, CHE and NIH3T3 cell lines. Quite similar changes were observed in three cell lines, but the Jurkat cells showed more sensitive than others. It was only 25 μl/plate THH that induced the high peak of MN formation at various time points, and only 50 μl/plate that induced the high peak of cell apoptosis. Conclusion　The result indicates that THH has stronger toxicity to Jurkat cells than to CHE and NIH3T3 cells.

Objective To investigate the genetic effect of low power microwave radiation on the anomaly of sperm and micronucleus(MN) formation in the marrow polychromatic erythrocyte (PCE) in rat. Methods Rats were randomly divided into three groups, namely control, experimental group Ⅰ and experimental group Ⅱ. Abnormal sperms and MN formation in marrow PCE were observed and detected after exposure to 1 800 MHz (Continuous wave, CW) microwave radiation for 21 days (12 h/d) with the average power density of 0 mW/cm 2 (control), 0 5 mW/cm 2 (group Ⅰ) and 1 0 mW/cm 2 (group Ⅱ). Results No significant difference in the rate of sperm abnormality and MN formation in marrow PCE was found between the experimental groups and the control. Conclusion No obvious genetic damage caused by low power microwave radiation to the sperm anomaly and MN formation in marrow PCE of rats is found.

Objective To determine the content of capsaicin in different kinds of dried capsicums.Methods A RP-HPLC method was established.The chromatographic column was DiamonsilTM C18(250 mm ? 4.6 mm,5 ? m) and the column temperature was 30 ℃.The mobile phase consisted of methnol ∶ 0.1 % phosphoric acid aqueous solution(70 ∶ 30) with a flow rate of 1.0 mL? min-1.The detection wavelength was 281 nm.Results A good linearity of capsaicin was in the rang of 0.100 6～ 4.024 0 ? g.The average recovery was 101.18 % and RSD was 1.81 %(n=6).Conclusion The method can be used to determine the content of capsaicin in different kinds of dried capsicums.

Objective To study the clinical efficacy and advantages of tip-flexible semirigid ureterorenoscope combined with Holmium laser lithotripsy for the treatment of impacted upper ureteral calculi.Methods We retrospectively analyzed 75 cases of impacted upper ureteral calculi treated in our hospital between September 2015 and December 2017.There were 45 males and 30 females,aged from 35 to 65 years,mean (42.5 ± 3.5)years.All patients were diagnosed by B-ultrasound and CT with impacted upper ureteral calculi.The mean stone diameter was (1.6 ± 0.3) cm.The CT value of stone was (1 025.3 ± 215.4) HU.Tip-flexible semirigid ureterorenoscope combined with Holmium laser lithotripsy were performed in all of the cases,which were under general anesthesia with lithotomy position,to observe the successful rate of lithotripsy,operation time,intraoperative and postoperative complications,postoperative hospitalization time and stone-free rate.Results All 75 cases were successfully operated.The operation time was (38.5 ± 11.2) min.Three cases had postoperative fever.No severe perioperative complications such as septic shock,ureteral perforation or avulsion occurred.The mean hospital stay of the patients was (2.7 ± 0.4) d.A follow up by non-contrast CT scan was done 4 weeks after the procedure to evaluate the result.Sixty seven cases had stone clearances at the primary operation and the stone clearance rate was 89.3％ (67/75).Eight cases undergone ESWL and physical vibration lithecbole,the CT examination showed that the stones were cleared after 1 month.Conclusions Tip-flexible semirigid ureterorenoscope combined with Holmium laser lithotripsy in the treatment of impacted upper ureteral calculi had a definite effect,safe and comfortable for doctors.

Surgical operation is the main treatment of oral and maxillofacial tumors.Dysphagia is a common postoperative complication.Swal-lowing disorder can not only lead to mis-aspiration,malnutrition,aspiration pneumonia and other serious consequences,but also may cause psychological problems and social communication barriers,affecting the quality of life of the patients.At present,there is no systematic evalua-tion and rehabilitation management plan for the problem of swallowing disorder after oral and maxillofacial tumor surgery in China.Combining the characteristics of postoperative swallowing disorder in patients with oral and maxillofacial tumors,summarizing the clinical experience of ex-perts in the field of tumor and rehabilitation,reviewing and summarizing relevant literature at home and abroad,and through joint discussion and modification,a group of national experts reached this consensus including the core contents of the screening of swallowing disorders,the phased assessment of prognosis and complications,and the implementation plan of comprehensive management such as nutrition management,respiratory management,swallowing function recovery,psychology and nursing during rehabilitation treatment,in order to improve the evalua-tion and rehabilitation of swallowing disorder after oral and maxillofacial tumor surgery in clinic.