Spleen deficiency and phlegm dampness syndrome is the common syndrome of the chest blockage.In this article,the pathological characteristics,syndrome characteristics and treating principle of the spleen deficiency and phlegm dampness syndrome of chest blockage were discussed.

BACKGROUND:Fibroblast growth factor can promote proliferation of mesenchymal stem cells (MSCs).grew along the wall.However,there are few reports on the differentiation of MSCs into hepatocytes following fibroblast growth factor induction.When mass concentration of hepatocyte growth factor was 1 μg/L,it can promote mitosis of hepatocytes,and is the strongest mitogenic agent for normal hepatocytes.OBJECTIVE:To explore the biological characteristics of human umbilical cord MSCs/n vitro and their differentiation ability to hepatocyte-like cells under the induction of chemical factors.DESIGN,TIME AND SETTING:The cytological in vitro study was conducted at the Institute of Blood,Jinan University from August 2008 to April 2009.MATERIALS:Umbilical cord was obtained from healthy full-term fetus,which was provided by the Guangzhou Huaqiao Hospital.The parturient signed informed consent.Hepatocyte growth factor and flbroblast growth factor were bought from Peprotech,USA.METHODS:The MSCs from human cord were isolated and cultured by type Ⅳ colagenase digestion+differential adherence.At the third passage,MSCs received cell surface antigen analysis and cell cycle determination to detect their ability to differentiate into adipocytes and osteoblasts.At the fifth passage,MSCs were adjusted into 5×10~9/L and assigned into 2 groups.BMSCs in the control group were incubated in DMEM/F12 containing 5% fetal bovine serum.BMSCs in the induction group were treated with above-mentioned medium supplemented with 20 μg/L hepatocyte growth factor and 10 μg/L flbroblast growth factor.MAIN OUTCOME MEASURES:The following parameters were measured:biological characteristics of human umbilical cord MSCs and differentiation of human umbilical cord MSCs into hepatocyte-like cells in vitro.RESULTS:At the third passage,MSCs derived from human cord expressed CD29,CD44,CD105,but not antigens of hematopoietic CD34,CD45,and 92.2% of them were in G_0/G_1 phase.Oil red O staining showed cytoplasm presented red granules.Alkaline phosphatase staining demonstrated that cytoplasm was black,with the differentiation ability into adipocytes and osteoblasts.Following 10 days of combined induction of hepstocyte growth factor and fibroblast growth factor,RT-PCR and Western blot results confirmed that cells expressed alpha fetoprotein and albumin.Negative expression was found in the control group.CONCLUSION:Human umbilical cord contained plenty of MSCs,with strong potential of multi-differentiation.Umbilical cord MSCs can differentiate into hepatocyte-like cells following combined induction of hepatocyte growth factor and fibroblast growth factor.

Objective With Dongcheng District residents for research, we used questionnaires, personal interviews, expert evaluation, systems analysis to study the health education mode of traditional Chinese medicine, and provide overall planning, pilot, summary of adjusted step by step implementation of final evaluation. In the adoption of a three-year health research and practice of traditional Chinese medicine, we have achieved good results. Area of traditional Chinese medicine culture has been strengthened, traditional Chinese medicine theory and appropriate technology have been widely used in the resident wellness activities. An appropriate health education mode of health education of traditional Chinese medicine is significant to highlight features of traditional Chinese medicine.

Based on the subtle discussion of changing rules and its principles of Six Qi movement recorded by one chapter named Liu Wei Zhi Da Lun in the Huang Di Nei Jing Su Wen about rules of Heaven divided by Six, Six Qi and year evolutive phase, Six Qi dominating the time, and guest climatic Qi adding to fixed host Qi, etc, this article explained the changes of influence of Heaven space-time on the human body from the angle of image thinking, focused on the key point of humanity conforming to the nature of the core of Chinese philosophy, and appealed readers to come back to the original thinking of China-image thinking.

OBJECTIVE: To detect whether there is mixing of norfloxacin in loperamide hydrochloride capsules by UPLC-MS. METHODS: The separation of norfloxacin was performed on C18 column with column temperature set at 35 ℃, and the mobile phase was composed of acetronitrile and 0.1% formic acid (gradient elution) with a flow rate of 0.3 mL?min-1. The sample size was 10 ?L. ESI source was applied with capillary tube tension at 3.0 kV and ion source temperature at 110 ℃, and scan mode was multiple reaction monitor. RESULTS: It has been proved that there was norfloxacin in 2 of the total 5 batches of samples based on the data of retention time of RP-HPLC including ultraviolet spectra, the quasi-molecular ion and the second order mass spectra split fragments. CONCLUSION: The method is highly selective and sensitive, and can be used to detect whether norfloxacin has been illegally added into loperamide hydrochloride capsules.

Objective To investigate the influence of Wenyang-Yixin recipe(WYYXR) on NF-κB signal transduction pathway of myocardial ischemia-reperfusion injury in rats. Methods 32 SD rats were divided into 4 groups randomly, sham-operated group, model group, western medicine control group and WYYXR group. Sham-operated group and model group were given physiological saline solution (10 ml/kg),estern medicine control group were treated with fluvastatin (4 mg/kg), and self-made Chinese herb was perfusedinto rats in WYYXR group (1 ml/100 g) each time, once a day, for successive three weeks before model making.The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats. NIK, IKKβ and IκBα protein expression of myocardial tissues in rats were detected by western blotting method. And the levels of NF-κBp65mRNA were determined with RT-PCR. Results Compared with model group, the expressions of NIK, IKKβ and NF-κBp65mRNA were lower, and the protein expression of IκBα was increased in WYYXR group, there were significant differences between the two groups (P=0.004> 0.003, 0.018, 0.004,P<0.05) . Conclusion The Wenyang Yixin Recipe can protect myocardium from MI/RI.

Objective To analyze the problems of treating plague by TCM and find out the characteristics of clinical research of contemporary plague by the analysis of clinical research literature on the treatment of plague by TCM. Methods Differential treatment methods of one kind of plague in the literature as the main source of the data and the information that the pathogenesis-symptom-location of disease-nature of disease-prescriptions-drugs were taken to build the data base of the syndrome differentiation and treatment of certain kind of plague. Results Analyzed with literatures,the characteristics of plague clinical research showed: Different pathogenesis characteristics and evolution of plague were caused by diff① erent evils. ②Contemporary plague was named by western disease name. A new model which disease differentiation combined with syndrome differentiation has been formed. ③The diversification of administration promoted the treatment of emergent communicable disease with Chinese medicine. Conclusion The plague research lacked such research literature which could both reflect individualized diagnosis and embody the clinical rules. Traditional Chinese medicine theory could be researched and enriched by finding out the pathogenesis characteristics and evolution rule of the new plague and thus guide the clinical practice. It was effective to control and treat mergent communicable disease with TCM.

In the circumstance of information age,this article made an analysis on the publish and distribution channels of medical professional literature.With the prosperity and development of the publishing industry,and diversified distribution channels,medical professional literature personnel must comply with the pace of the times,actively extended interview channels.

The user information needs, being one of the core elements in modem library, is the basis for survival and development of modern library. In the digital and network environment, the electronic resources are increasingly becoming the main part of information resources. The Library of China Academy of Chinese Medical Sciences is the central library of traditional Chinese medicine (TCM) in China, and therefore how to use limited funds to build an optimal, most rational and sustainable development guarantee system of TCM electronic resources is its new task in the information age. This paper discussed the objective and principles of building guarantee system of TCM electronic resources, and also put forward some measures and strategies about constructing it from eight aspects.

Our study was based on the book Theory of Epidemic Heat Syndrome. This book was written by Mr. Shao Dengying of Qing dynasty, who was famous in Qing Dynasty on curing virus infection. We described his unique insights in diagnosis and treatment of epidemic heat syndrome in six aspects: the origin, position of epidemic heat invasion, difference between epidemic heat syndrome and typhus, paying attention to the symptoms of epidemic heat, differentiation and treatment from qi, bloods and stomach, and attaching importance to the stomach Qi and kidney essence etc.