AIM: The changes of myocardial nuclear membrane Ca 2+ -ATPase function was investigated in ischemia/reperfusion injury. METHODS: The model of myocardial ischemia/reperfusion injury was established in rats. Myocardial nuclei were purified with sucrose density centrifugation,the activity of Ca 2+ -ATPase was measured and calcium uptake was assayed with [ 45 Ca 2+ ] . RESULTS: Plasma levels of malondialdehyde (MDA) and free fatty acid (FFA) in myocardial ischemia/reperfusion injury increased significantly( P

Objective To study the regulatory effects of Ca 2+ , ATP, ADP and AMP on myocardial nuclear Ca 2+ ATPase activity after ischemia reperfusion injury Methods The model of myocardial ischemia reperfusion injury was established in rats Myocardial nuclei were purified using sucrose density gradient centrifugation The activity of Ca 2+ ATPase was measured with ascertaining phosphate Results Compared with the control, plasma levels of MDA and FFA after myocardial ischemia reperfusion injury increased significantly ( P

Objective To establish a method for determination of 3’-chloromethylacetophenone in the air of ketoprofen workplaces. Methods The 3’-chloromethylacetophenone was sampled using activated carbon tube and then desorbed by ethyl acetate. All the components were determined by gas chromatograph with FID after complete separation with FFAP capillary column. Results This method presented a linear relation within 5-1 000 ?g/ml, r value was 0.999 2. The detection limit was 1.67 mg/m3. Desorption efficiencies were 92.2%-101.5%. The break through volume of 100 mg of activated carbon was 5.0 mg. Conclusions The present method is feasible for determination of 3’-chloromethylacetophenone in workplace air.

Objective To explore the clinical curative effect and safety of intra -abdominal beacizumab bead sheet resistance combined with hyperthermic intraperitoneal chemotherapy in treatment of ovarian cancer perito-neal effusion.Methods 60 patients with ovarian cancer peritoneal effusion in Binzhou Central Hospital from June 2012 to July 2015 were randomly divided into two groups.30 cases in the control group were treated with intraperito-neal hyperthermic perfusion chemotherapy, while 30 patients in the observation group were given intra-abdominal beacizumab bead sheet resistance combined with hyperthermic intraperitoneal chemotherapy.The short-term effica-cy,toxicity and quality of life were compared.Results The effective rate of the observation group was 73.33%, which was significantly higher than 53.33% in the control group,the difference was significant (χ2 =8.55,P<0.05).In the two groups, there were no significant differences on thrombocytopenia, reduced white blood cells and hemoglobin,nausea,vomiting and abdominal pain (χ2 =1.07,1.87,0.90,1.51,2.88 , all P >0 .0 5 ) .The improvement rate of life quality of the observation group was 76.67%,which was significantly higher than 50.00%of the control group,the difference was significant (χ2 =8.06,9.31,all P<0.05).Conclusion Intra-abdominal beacizumab bead sheet resistance combined with hyperthermic intraperitoneal chemotherapy has remarkable curative effect in treatment of ovarian cancer peritoneal effusion,with high safety,and can significantly improve the quality of life of patients,that is suitable for clinical application.

Aim Observing the alteration of cardiac myocyte nuclear inositol 1,4,5-trisphosphate receptor (IP_3R)binding proterties in rat subjected to myocardium ischemic reperfusion is to make it clear whether this change is involved in the molecule mechanism of cell apoptosis of rat with myocardial ischemic reperfusion. Method Apoptosis index of myocardial cell was determined using TUNEL assay.Extracting of cardiac myocyte nucleus was accomplished by saccharose density gradient centrifugation method,the binding proterties of nuclear IP_3R in different conditions were detected by radioligand binding assay.Results ①Myocardial cell apoptosis index in rat heart underwent 30 min regional ischemia and 3 h reperfusion was distinctly increased compared with sham-operated group(P

AIM: To investigate the changes in nuclear calcium content and permeability of nuclear pore complex in rat myocardium during ischemia reperfusion injury.METHODS: The rat model of myocardial ischemia reperfusion injury was established.Myocardial nuclei were purified using sucrose density gradient centrifugation.The nuclear calcium content was measured by atomic absorption spectrophotometer.The permeability of nuclear pore complex was assessed through measuring the amount of calmodulin conjugated Alexa Fluo~(TM) 488 as fluorescent probes transported across nuclear membrane with spectrofluorometer.RESULTS: The nuclear calcium content at 15,30,60,120 and 180 min reperfusion following 30 min sustained ischemia increased 1.31-,1.55-,1.73-,1.94-and 2.14-fold,respectively,as compared with sham-operation group.The permeability of nuclear pore complex at 15 min reperfusion following 30 min sustained ischemia showed no difference from sham-operation group,but it only increased 1.31-,1.38-,1.40-,and 1.48-fold at 30,60,120 and 180 min reperfusion following 30 min sustained ischemia compared with sham-operation group.CONCLUSION: The nuclear calcium content during myocardial ischemia reperfusion injury increases earlier than the permeability of nuclear pore complex does.The increase in the permeability of nuclear pore complex may result in adaptive regulatory effects on nuclear calcium overload to a certain extent during myocardial ischemia reperfusion injury.

Objective To study the expression and clinical significance of serum levels of transforming growth factor-31 (TGF-β1) and tumor necrosis factor-alpha(TNF-α) in patients with hypertensive disorder complicating pregnancy(HDCP).Methods The serum levels of TGF-β1 and TNF-αin 50 cases with HDCP and 30 cases of normal third trimester pregnant women(control group) were detected by ELISA.50 cases with HDCP were divided into gestational hypertension group,mild preeclampsia group and severe preeclampsia group according to the severity of HDCP.Results The serum levels of TGF-β1 and TNF-α in HDCP patients were significantly higher than the control group (t =13.283,13.607,all P ＜ 0.05).The serum levels of TGF-β1 and TNF-α gradually increased with the aggravating of HDCP disease,the serum levels of TGF-β1 and TNF-αwere significantly different among the three subgroups of HDCP(P ＜ 0.05).The correlation analysis showed that the serum levels of TGF-β1,TNF-α were positively correlated with the severity of HDCP (r =0.575,0.512,all P ＜ 0.05),there was significantly positive correlation between the serum TGF-β1 and TNF-α(r =0.515,P ＜0.05).Conclusion The serum levels of TGF-β1 and TNF-α were high in the HDCP patients,the high levels of TGF-β1 and TNF-α can reflect the changes and development of HDCP disease.The abnormal levels of TGF-β1 and TNF-α may be associate with dysfunction of trophoblast cell.

[Objective] To observe the efficacy of metformin treatment on non-alcoholic fatty liver disease (NAFLD) in obese children.[Methods] A retrospective analysis was performed of 10 patients over 10 years old with NAFLD from July 10,2013 to August 23,2016.These patients were treated with metformin in pediatric endocrinology outpatient department of the First Affiliated Hospital of Sun Yat-sen University.The changes of liver ultrasonography,hepatic enzymes,blood lipids,blood glucose,insulin,HOMA-IR,BMI,and waist circumference height ratio were compared before and after treatment with metformin.[Results] There were 10 cases of NASH,including 5 boys and 5 girls.The short-term treatment of metformin reduced the levels of ALT,AST,and HOMA-IR for all 10 patients (P ＜ 0.01).ALT,gradually decreased with the course of treatment.Fasting insulin and waist circumference to height ratio also improved with the treatment (P ＜ 0.05);the changes of TG,BMI,and fast glucose were not obvious (P ＞ 0.05).[Conclusion] Metformin can effectively reduce liver enzymes and improve insulin sensitivity in children with NASH in short term,the improvement of TG and BMI in short term is not obvious.

[ ABSTRACT] AIM:To investigate the effect of combined treatment with gonadotropin-releasing hormone analogue ( GnRHa) and growth hormone ( GH) on the linear growth in mid-and late pubertal girls at great bone ages with central precocious puberty ( CPP) or early and fast puberty ( EFP) , and to determine the relation between C-type natriuretic pep-tide ( CNP) signaling pathway and the accelerative effect of GH on long bone growth in these girls.METHODS:Twenty-two girls were diagnosed as CPP or EFP, whose bone ages were older than 11.5 years with impaired predicted adult height ( PAH) , and divided into GnRHa treatment group ( treated with GnRHa alone, slow-release of triptorelin 60~80 μg/kg every 4 weeks, im) and combined treatment group ( treated with GnRHa and GH, 1 U/kg GH every week for 6~7 times, sc) .The height, weight and pubertal stage were determined every 3 months.At the beginning and after 6 months of the treatment, the bone age was evaluated and the serum concentrations of amino-terminal pro-C-type natriuretic peptide ( NT-proCNP), insulin-like growth factor 1 (IGF-1) and procollagen type 1 amino-terminal propeptide (P1NP) were measured. Height velocity ( HV) , height SD score for bone age ( HtSDSBA ) , PAH and the serum indexes mentioned above were com-pared at the beginning and the end of the treatment.RESULTS: After 6 months of the treatment, HV, ΔHtSDSBA andΔPAH of the girls treated with GnRHa +GH were statistically higher than those of the girls given GnRHa alone ( P <0.01).Serum concentrations of NTproCNP, P1NP and IGF-1 were not significantly different between the beginning and the end of the 6-month combined treatment.The girls treated with GnRHa alone showed a significant decrease in both serum NTproCNP and P1NP levels (P<0.05) and no significant change of serum IGF-1 level after 6 months of the treatment. CONCLUSION:In the CPP or EFP girls who are in mid-and late puberty and at great bone ages, the combined treatment with GnRHa and GH may accelerate linear growth and improve predicted adult height.This effect of GH is not attributed to the change of serum IGF-1 level, and may be related in part to the acceleration of CNP-mediated long bone growth.

[Objective] We assessed in a retrospective unicenter study the state of metabolism and gonadal axis of early menarche girls and girls who treated with Gonadotropin-releasing hormone analogs (GnRHa).[Methods] Thirty-nine early menarche girls and 58 girls who had been treated with GnRHa were enrolled in our study and 19 normal menarche girls were enrolled as control group.Data were collected in height,weight,gonadal hormone,blood glucose,insulin,blood lipid,leptin,adiponectin and the size of uterus and ovary.[Results] Both BMI SDS for chronological age (CA) and for bone age (BA) of early menarche girls were significantly higher than normal menarche girls (P ＜ 0.05).The ratio of insulin resistance in early menarche girls (20.5％) was also significantly higher than normal girls (0％).No significant difference in lipid metabolism and gonadal axis between two groups.In girls treated with GnRHa,BMISDS,insulin,HOMA-IR and the ratio of insulin resistance (20.7％) were all significantly higher than normal group (P ＜ 0.05).Meanwhile,DHEAS,androstenedione and testosterone of GnRHa treated girls were significantly higher than early menache girls,and DHEAS was higher than normal girls.The size of uterus in treated group was larger than the other two groups.[Conclusion] Early menarche and GnRHa treatment may take negative effect to BMI and glucose metabolism.Androgen was higher in GnRHa treated group.Therefore,suggestion was that BMI,insulin,blood glucose and androgen should be monitored in early menarche girls and girls treated with GnRHa.