Objective To assess the clinical effects of uterine arterial embolization on uterine leiomyoma.Methods We treated 21 patients with uterine leiomyoma by Seldinger's uterine arterial embolization. The 4.0 F Cobra catheter was placed into the double uterial arteries with Polyvinyl Alcohol Foam(PVA) particles as the embolizer. At 3 and 6 months postoperatively, we investigated the effects of the embolization. Results After uterine arterial embolization, the volume of uterine leiomyoma decreased and menorrhagia was under controlled. The symptoms of anemia and oppression were relieved. The physiologic functions of ovary and uterus were preserved completely.Conclusions Uterine arterial embolization is an effective and less invasive way to treat uterine leiomyoma.

Objective To examine the effect of donepezil on cognitive function of Alzheimer's disease from a naturalistic observation in memory clinic.Methods From the subject pool in memory clinic,thirteen patients with mild to moderate Alzheimer's disease(meeting NINCDS-ADRDA criteria of probable AD)were enrolled in the naturalistic observation.Donepezil with dosage of 5 mg daily Was administered.Cognitive Ability Screening Instrument (CASI)Was used to assess the cognitive function,and was administered at baseline,3 and 6 months after treatment with donepezil.Resuits After 6-month treatment with donepezil,the change of score of verbal fluency from baseline Was significant[(4.3±3.1)vs(6.2±2.7),P＜0.05].There was a trend that constructional praxis was also improved after 6-month treatment(P=0.06).The change of scores of other cognitive domains remained the sanle(P＞0.05).Conclusion In the naturalistic observation at memory chnic,donepezil may improve verbal fluency of AD patients,and keep most cognitive function stable.

Objective: To determine the cognitive function and MRI-based measurement of MTL structures in patients with Alzheimer disease (AD) and to find a sensitive cognitive marker or specific MRI-based measurements for early diagnosis of AD. Method:Fifty-two AD patients, 27 VD (vascular dementia) patients and 35 aged matched normal control were recruited. The cognitive functions were evaluated with Alzheimer disease assessment scale-Cognitive portion (ADAS-Cog), Wechsler memory scale-revised and Mini-Mental Status Examination (MMSE). MTL structures, including hippocampal formation, amygdala and parahippocampal gyrus, were measured by MRI volumetric measurements. Result: Compared with VD patients and healthy controls, there was more pronounced reduction of performance on the main cognitive domains in AD patients, which indicated the diffused deficits of AD, including learning and memory, comprehension, language, orientation and concentration. The atrophy of MTL structures was more prominent in AD patients. A moderate diagnostic accuracy of 83.3% was achieved by Fisher's linear discriminant model, which comprised the scores of word recall, recall of test instructions, commands, constructional praxis and orientation in ADAS-Cog, and the performance on picture recall, visual recognition visual reproduction, logic memory and touch test in Wechsler memory test and part of memory in MMSE. In diagnose of AD, MRI based volumetry of left temporal horn of lateral ventricle and amygdalohippocampal complex had the sensitivity of 78.4% and accuracy of 77.1%. When cognitive indicators were combined with MRI based measurement, the diagnostic accuracy increased to 90.5%. Conclusion: AD produces more diffused cognitive deficit and severer atrophy of MTL structure. Combination of cognitive indicator with MRI based measurement can improve accuracy in diagnosis of AD.

Objective:To examine the discriminatory potential of the ADAS-Cog, Chinese version for mild and moderate Alzheimer's disease (AD). Methods:Patients with AD (199 mild AD and 106 moderate AD) meeting the NINCDS-ADRDA criteria of probable AD were recruited. The Chinese version of ADAS-Cog was administered to all AD subjects. Results:The total score of ADAS-Cog and the score of each ADAS-Cog item differed significant-ly between mild and moderate AD groups, with higher scores in moderate AD group. GLM analysis showed insignifi-cant influence of age or educational level on the ADAS-Cog total score. These results indicated that ADAS-Cog could represent the distinctive profiles of cognitive impairment between mild and moderate AD. The results of Logis-tic regression analysis showed that the item score of orientation and constructional praxis as well as the ADAS-Cog total score could classify mild and moderate AD efficiently, with a sensitivity of 78% - 82% and a specificity of 70% - 73%. Conclusion:Our results indicate that the Chinese version of ADAS-Cog is useful for staging of AD. It is recom-mended that the Chinese version of ADAS-Cog be introduced for monitoring the AD drug therapeutic efficacy on cognitive impairment among Chinese AD patients.psychiatry Alzheimer's disease

Objective: To investigate the effect of Nano-Se with lower toxicity on the anti-tumor effect of cisplatin. Method: For toxicity comparison, mice were orally administered Nano-Se and sodium selenite to observe death, body weight loss and liver injury. As to the protective effect of Nano-Se on the toxicity of cisplatin, mice with and without Nano-Se supplementation were injected cisplatin to observe death and body weight loss. For evaluation of whether Nano-Se influence the anti-tumor effect of cisplatin, Lewis lung carcinoma was transplanted into mice, and the survivor and tumor volume were recorded to compare the effect of Nano-Se supplementation on cisplatin. Results: The toxicity of Nano-Se is significantly lower than sodium selenite. Nano-Se can decrease toxicity of cisplatin without compromising its anti-tumor effect. Conclusion: Nano-Se with lower toxicity can improve the therapeutic effect of cisplatin.

OBJECTIVE: To estimate the prevalence of allergic rhinitis (AR) in a community center in Beijing. METHOD: We randomly investigated a community with 13 900 inhabitents in Beijing by means of questionnaire survey. RESULT: A total of 2000 questionnaires was send out, and 1988 of them were collected, which included 952 males and 1036 females (age: 1 to 96 years old, 128 cases are younger than 14 years old. Among of them, 194 cases (9.76%) were diagnosed as having AR according to epidemiologic definition, including 14 children. The prevalence of AR was 9.68% in adults, with male 10.21% and female 9.18%, 10.94% in children. The prevalence in male are slightly higher than in female both with adults and children but without statistic significance. CONCLUSION The survey showed the incidence of AR in Beijing are 9.76% in population, adults 9.68%, children (younger than 12) 10.94%. AR patients are more likely to have other allergic diseases.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; China ; epidemiology ; Cities ; epidemiology ; Female ; Humans ; Hypersensitivity ; epidemiology ; Incidence ; Infant ; Male ; Middle Aged ; Prevalence ; Rhinitis, Allergic ; epidemiology ; Sex Distribution ; Surveys and Questionnaires

Objective:With selenomethionine as reference,we investigated the bioavailability and toxicity of methylselenocysteine. Method:Selenium retention,glutathione peroxidase and thioredoxin reductase activities were tested to compare bioavailability. Acute toxicity and short-term selenium-tolerance were studied to compare toxicity. Results:Selenomethionine and methylselenocysteine were comparable in ability of increasing selenoenzymes. Selenomethionine caused much higher selenium retention,especially at higher levels and lower toxicity indicated by acute toxicity and short-term selenium-tolerance. Conclusion:Compared with selenomethionine,methylselenocysteine has equal efficacy on selenoenzymes with the characterization of less retention,but higher toxicity.

Objective The protein kinase C ( PKC) is an essential signaling substance in the early protection of cells in pre-conditioning.This study was to investigate the role of PKC in the myocardial cells of the rat model of anoxia -reoxygenation (A-R) in-jury preconditioned with sufentanil . Methods Primary myocardial cells isolated and cultured for 5 days were allocated to a control , an A-R, a sufentanil preconditioning ( SF) , and a phorbol+sufentanil preconditioning ( PMA +SF) group.The A-R injury model was established with cultured myocardial cells from neonatal rats , which were preconditioned with sufentanil at the concentration of 0.000 3 μmol/L in the SF group or phorbol followed by sufentanil 10 minutes later in the PMA +SF group.Then the proliferation of the cells was detected , the optical density of the Cx 43 protein observed by immunofluorescence confocal technology , the apoptosis rate of the cells determined by flow cytometry, and the total Cx43 proteins calculated by Western blot. Results Cell proliferation was signifi-cantly increased in the A-R, SF, and PMA+SF groups as compared with the control (P<0.05), higher in the SF and PMA +SF than in the A-R group (0.498 0 ±0.0432 4 and 0.7240 ±0.1234 vs 0.325 8 ±0.023 5, P<0.05), and in the SF than in the PMA+SF group (P<0.05).Flow cytometry showed significantly increased rates of early , late, and total cell apoptosis in the A -R ([4.96 ±0.59], [18.77 ±0.92], and [23.73 ±0.51]%), SF ([5.86 ±0.38], [10.37 ±0.38], and [16.23 ±0.32]%), and PMA+SF group ([5.71 ±0.58], [5.54 ±0.43], [11.24 ±0.62]%) as compared with the control (P<0.05), remarkably lower in the SF and PMA +SF than in the A-R group (P<0.05), and the late and total cell apoptosis rates markedly lower in the SF than in the PMA+SF group (P<0.05). Conclusion Sufentanil has a protective effect and PKC agonists combined with sufentanil may add to the effect on myocardial cells in A -R injury.

Objective:To establish the quantitative method for eucalyptol in Chimonanthus salicifolius S. Y. Hu by GC. Methods:The GC method was performed on a Zebron ZB-WAX column (60 m × 0. 32 mm,0. 5 μm) with programmed temperature of 60-200℃, an FID detector was used, the detector temperature was 250℃, the inlet temperature was 220℃, and the carrier gas was nitrogen with high purity. Results:A good linearity of eucalyptol was within the range of 0. 012 6-0. 503 4 mg·ml-1(r=0. 999 8), and the average recov-ery was 100. 68%(RSD=1. 51%,n=6). Conclusion:The method is simple, accurate and quick with good reproducibility, and suitable for the quality control of Chimonanthus salicifolius S. Y. Hu.

Objective To label rat bone marrow mesenchymal stem cells (BMSCs) with superparamagnetic iron oxide in vitro, and to monitor the survivorship and location of the labeled BMSCs in rat models of traumatic brain injury (TBI) with high field MR. Methods BMSCs were cultivated in vitro, and were labeled with SPIO. TBI models were built in the left hemisphere of the rats with Feeney's method. Then SPIO-labeled BMSCs were grafted stereotactically into the region nearby the contusion site 24 h later. The rats underwent MR examination 1 day, 3 days, 1 week and 3 weeks after implantation. Results Brown iron particles could be demonstrated in the SPIO-labeled BMSCs under inverted phase contrast microscope. Numerous intracytoplastic iron particles were stained with Prussian blue, and diffused distribution of iron particles could be seen in the intracytoplasm under electron microscope. At implanted sites low signal intensity could be observed on every sequence of MR examination, among which T2~*WI and SWI were better than other sequences, and SWI was the best. Conclusion MR is sensitive of tracking the survivorship and location of the labeled BMSCs, and SWI is the most sensitive sequence to detect the labeled cells.