OBJECTIVE To study the pharmacokinetics and tissue distribution of deferiprone (DFP) in rats. METHODS Plasma and tissues were collected after male Wistar rats were ig given DFP 35, 70 and 140 mg·kg~(-1) at different time points. The DFP in plasma and tissues was determined by high performance liquid chromatography. The compartment model was fitted and pharmacokinetic parameters were calculated by DAS 2.0. RESULTS The results showed that the pharmacokinetic process of DFP in rats was two-compartment model after rats were ig given DFP 35, 70 and 140 mg·kg~(-1). The t_(1/2α) were 23.3, 22.2 and 20.9 min, respectively. The t_(1/2β) were 53.3, 50.9 and 46.3 min, respectively. The Cl were 0.017, 0.021 and 0.016 L·min~(-1)·kg~(-1), respectively. The content of DFP was high in stomach and liver tissues after rats were ig given DFP 70 mg·kg~(-1), and it was lower in the other tissues. The content of DFP in liver tissues was (359.22±31.16)μg·g~(-1), at 60 min after rats were ig given DFP 70 mg·kg~(-1). CONCLUSION The absorption and elimination of DFP are quick and the tissue distribution of DFP is wide in vivo.

Objective To explore the roles of zinc-fingers and homeoboxes (ZHX) in the dorsal root ganglia (DRG) in peripheral nerve injury-induced pain hypersensitivity,and provide a new idea for molecular mechanism ofneuropathic pain (NP).Methods (1) Twenty-four 8-week-old male C57BL6 mice were randomly divided into sham-operated group and chronic constriction injury (CCI) of the sciatic nerve model group (n=12).One d before modeling and 7 d after modeling,the changes of paw withdrawal frequency (PWF) to mechanical stimuli and paw withdrawal latency (PWL) to thermal stimulation were detected.Seven d after modeling,reverse transcription real-time quantitative PCR (RT-qPCR) was used to detect the ZHX1,ZHX2,and ZHX3 mRNA expressions,and Westem blotting was employed to detect the ZHX2 protein expression.(2) Thirty-six 8-week-old male C57BL6 mice were randomly divided into equivalent dose solvent CCI group,nonsense negative control sequence [siNC]CCI group and ZHX2 siRNA CCI group,and equivalent dose solvent sham-operated group,siNC sham-operated group and ZHX2 siRNA sham-operated group (n=6);each treatment was given to the DRG.One d before drug injection and 7 d after drug injection,the changes of PWF to mechanical stimuli and PWL to thermal stimulation were detected;RT-qPCR was used to detect the ZHX2 mRNA expression.Results (1) Seven d after modeling,PWF was significantly increased and PWL was statistically shorten in the hind-paw of the ipsilateral side in the CCI group as compared with those in the sham-operated group (P＜0.05);DRG ZHX2 mRNA expression in the CCI group increased for 1.71 times as compared with that in the sham-operated group,with significant difference (P＜0.05);DRG ZHX2 protein expression in the CCI group increased for 2.15 times as compared with that in the sham-operated group,with significant difference (P＜0.05).(2) As compared with the equivalent dose solvent sham-operated group,siNC sham-operated group and ZHX2 siRNA sham-operated group,equivalent dose solvent CCI group and siNC CCI group had significantly increased PWF and DRG ZHX2 mRNA expression,and statistically shorten PWL (P＜0.05);and ZHX2 siRNA CCI group had significantly decreased PWF of the ipsilateral side and DRG ZHX2 mRNA expression,and statistically longer PWL of the ipsilateral side as compared with equivalent dose solvent CCI group and siNC CCI group (P＜0.05).Conclusion Knockdown periphery nerve injury-induced DRG ZHX2 up-regulation attenuates pain hypersensitivity following periphery nerve injury,and ZHX2 may be a new potential target to treat NP.

To establish a simple,convenient,sensitive,and specific method for rapid detection of Zi-ka virus(ZIKV),the whole genome sequences of ZIKV isolated from different times and regions were analyzed.The specific primers and probes were designed based on the screened target se-quences located in the conserved region of the ZIKV NS5 gene.By combining RT-LAMP isother-mal amplification technology and immunochromatography technology,a reverse transcription loop mediated isothermal amplification nucleic acid and flow visualization strip(RT-LAMP-VF)detec-tion method for ZIKV was established.The results showed that the method had good specificity and sensitivity.When the ratio of inner,outer,and ring primers(FIP∶LF∶F3)was 4∶2∶1,the detection method can specifically detect 102 copies/pL RNA transcripts or 2.15 pfu ZIKV at 61 ℃for 45 minutes,with no cross reaction with other flaviviruses such as Japanese encephalitis virus and classical swine fever virus.Other RNAs in blood tissue samples did not affect the sensitivity and specificity of RT-LAMP-VF,indicating that the method can be applied to clinical practice.The ZIKV RT-LAMP-VF detection method established in this study is easy to perform and does not require special instruments and equipment.It is particularly suitable for the rapid detection of ZIKV in grassroots units,providing technical support and material support for the establishment of on-site rapid detection and early warning and prediction systems for ZIKV disease.