Objective To investigate the distribution and chemical character of nestin immunoreactive cells in the human basal forebrain. Methods We explored systematically the distribution of nestin immunoreactive cells throughout the human basal forebrain by nestin 331B, 10C2, Rat401 antibody.Furthermore, we investigated the chemical identity of these nestin immunoreactive cells by using double\|labeling immunocytochemistry with NSE, ChAT, p75NGFR, GFAP antibody or using NADPH\|d histochemistry. Results The nestin immunoreactive cells were found in the septum, diagonal band of Broca, innominate substance,amygdala,basal nucleus of Meynert of adult human brain.These nestin immunoreactive cells haven big cell body,2\|3 processes.The nestin immunoreactive cells were labeled by NSE antibody.The large majority of those were single stained,and 28% were double labeled with ChAT positive neurons,15% with NGFR positive neurons,6% with NOS positive neurons.A few nestin immunoreactive cells shared similar morphology with that of astrocyte glia which existed in the spaces between the thin septa pellucida or the midline along the septum.It could not be labeled by GFAP antibody.Conclusion\ A new cluster of nestin immunoreactive neurons that were different from the ChAT,NGFR,NOS positive neurons existed in adult human basal forebrain.\;[

Pluronic modified polyamidoamine (PAMAM) conjugate (PF127-PAMAM) was prepared and the inhibiting effect of MDR against MCF-7/ADR was investigated with doxorubicin (DOX) as model drug. 1H NMR and FTIR spectra showed that the conjugate was synthesized successfully. Element analysis accurately measured that 27.63% amino of per PAMAM was modified by pluronic (PAMAM : PF127, 1 : 35.37 mole ratio). PF127-PAMAM showed an increased size and a reduced zeta potential compared to PAMAM. PF127-PAMAM had lower hemolytic toxicity and cytotoxicity due to the reduced zeta potential and the protection of PF127. Each PF127-PAMAM molecular could load 19.58 DOX molecules, and the complex exhibited sustained and pH-sensitive release behavior. PF127-PAMAM/DOX exhibited weaker cytotoxicity than free DOX in MCF-7 cells; while the complex showed much stronger reverse effect of drug resistance in MCF-7/ADR cells, and resistance reversion index (RRI) was as high as 33.15.

Objective To explore the effects of intervention model based on empowerment theory on health belief,self-care behavior and prognosis of patients with myocardial infarction after percutane-ous coronary intervention(PCI).Methods A total of 500 patients with PCI for myocardial infarction in the hospital from November 2021 to October 2023 were randomly divided into control group(n=250)and observation group(n=250).The control group was given routine intervention mode,the observation group was given intervention mode based on empowerment theory,and both groups were in-tervened until one month after discharge.Before and after intervention,the health belief[the Coronary Heart Disease Health Belief Scale(CADHBS)],self-care ability[the Evaluation for Self-care Ability(ESCA)],cardiac function indexes[left ventricular ejection fraction(LVEF)and 6 minutes walking test(6MWT)]and quality of life[the Chinese Questionnaire of Quality of Life in Chinese Patients with Cardiovascular Diseases(CQQC)]were compared between the two groups;the incidence rate of adverse cardiac events during intervention was compared between the two groups.Results Compared with the control group,the total score of CADHBS and the score of each dimension as well as the total score of ESCA and the score of each dimension in the observation group were significantly higher(P＜0.05).After intervention,the LVEF and 6MWT in the observation group were significantly higher than those in the control group(P＜0.05).The total incidence rate of adverse cardiac events was 19.60％in the observation group,which was significantly lower than 28.00％in the control group(P＜0.05).After intervention,the CQQC score in the observation group was significantly higher than that in the control group(P＜0.05).Conclusion For the patients with PCI for myocardial infarction,imple-mentation of intervention model based on empowerment theory can enhance health belief and self-care ability,improve the cardiac function and quality of life,and reduce the incidence rate of ad-verse cardiac events.

Objective To explore the effects of intervention model based on empowerment theory on health belief,self-care behavior and prognosis of patients with myocardial infarction after percutane-ous coronary intervention(PCI).Methods A total of 500 patients with PCI for myocardial infarction in the hospital from November 2021 to October 2023 were randomly divided into control group(n=250)and observation group(n=250).The control group was given routine intervention mode,the observation group was given intervention mode based on empowerment theory,and both groups were in-tervened until one month after discharge.Before and after intervention,the health belief[the Coronary Heart Disease Health Belief Scale(CADHBS)],self-care ability[the Evaluation for Self-care Ability(ESCA)],cardiac function indexes[left ventricular ejection fraction(LVEF)and 6 minutes walking test(6MWT)]and quality of life[the Chinese Questionnaire of Quality of Life in Chinese Patients with Cardiovascular Diseases(CQQC)]were compared between the two groups;the incidence rate of adverse cardiac events during intervention was compared between the two groups.Results Compared with the control group,the total score of CADHBS and the score of each dimension as well as the total score of ESCA and the score of each dimension in the observation group were significantly higher(P＜0.05).After intervention,the LVEF and 6MWT in the observation group were significantly higher than those in the control group(P＜0.05).The total incidence rate of adverse cardiac events was 19.60％in the observation group,which was significantly lower than 28.00％in the control group(P＜0.05).After intervention,the CQQC score in the observation group was significantly higher than that in the control group(P＜0.05).Conclusion For the patients with PCI for myocardial infarction,imple-mentation of intervention model based on empowerment theory can enhance health belief and self-care ability,improve the cardiac function and quality of life,and reduce the incidence rate of ad-verse cardiac events.