Objective To investigate the nutritional status of vitamin D (Vit D) in newborns in Xi'an, and to provide a theoretical basis for timely and targeted formulation of regional and individualized Vit D nutrition intervention strategies. Methods A cross sectional survey was performed in newborns within 24 hours life age at the neonatal department of China Xidian Group Hospital. Serum 25-hydroxyvitamin D (25(OH)D) levels were detected and compared for the mean among groups in accordance with gestational age, birth weight and gender. Multiple linear regression analysis was used to analyze the effect of each factor on serum 25 (OH) D levels. A P-value of P <0.05 was considered significant. Results A total of 582 newborns were recruited in this study. The average serum 25 (OH) D level within 24 hours after birth was 11.2±3.2ng/ml, which was a deficiency. The overall prevalence of Vit D level abnormality (insufficiency + deficiency + severe deficiency) was 96.70% (563/582 cases). The gestational age and birth weight had a significant effect on Vit D levels (β=2.94, P<0.001 and β=7.71, P<0.001, respectively), while the gender had no effect (β=-0.003, P>0.05). Conclusion Vit D nutritional deficiency existed in some newborns in Xi'an. Measures need to be taken to improve the status of newborn Vit D deficiency.