1.Advances in Agrobacterium tumefaciens-mediated transgenic cucumber.
Li'ang CHAI ; Huaifu FAN ; Chen LIU ; Changxia DU
Chinese Journal of Biotechnology 2020;36(4):643-651
Cucumber (Cucumis sativus) is an important vegetable crop in the world. Agrobacterium-mediated transgenic technology is an important way to study plant gene functions and improve varieties. In order to further accelerate the transgenic research and breeding process of cucumber, we described the progress and problems of Agrobacterium tumefaciens-mediated transgenic cucumber, from the influencing factors of cucumber regeneration ability, genetic transformation conditions and various additives in the process. We prospected for improving the genetic transformation efficiency and safety selection markers of cucumber, and hoped to provide reference for the research of cucumber resistance breeding and quality improvement.
Agrobacterium tumefaciens
;
metabolism
;
Breeding
;
Cucumis sativus
;
genetics
;
microbiology
;
Plants, Genetically Modified
;
microbiology
;
Research
;
Transformation, Genetic