AIM: To investigate the topography of the anterior surface of the human crystalline lens. ·METHODS: A Non-contact three-coodinate measuring system was utilized to scan the anterior surfaces of 8 human eye lenses in vitro. Acquired data were processed and computer models of the anterior lens surfaces were made using the program (Surfacer v 10.0). The to-pography of the anterior lens surfaces were established. Radii of curvature were measured at preset spots over the lens surfaces and two way analysis of variance was performed. The data were analysed to determine whether the radius of curvature varied systematically with the position on the lens surface from which the measurement was made. Lens surface asymmetric index (LSAI) was defined and calculated. The vertical and horizontal meridians of the modeled lens were calculated, and the best curve fit to any conic section was determined. ·RESULTS: The topography of the anterior lens surface indicated that the central zone (the central radius of curvature is (9.09±0.80)mm was steeper than that of the peripheral zone(17.05±2.20)mm. Two way analysis of variance of the radii of curvature at preset spots over the lens surfaces showed that the differences were statistically significant (P ＜ 0.05). Curve regression of radii of curvature at preset spots and their distances to the surface center revealed that the correlation of them was the third power function. LSAI increased steadily from the lens surface center(0.013±0.005) to the periphery(0.184± 0.065). The dots on the horizontal and vertical meridians were fit to four kinds of curves, and the determinate coefficient of hyperbola fit were the largest (0.9989-0.9999). ·CONCLUSION: The anterior lens surface is imperfectly rotational symmetric. Moreover, the nearer to the center, the more rotational symmetric it is. Radii of curvature increase nonlinearly from the surface center to the periphery. Anterior lens surface is typically hyperbolic.

AIM: To investigate the time - effect relationship between the expression of rhodopsin and recoverin and photoreceptor damage induced by N - nethl - N -nitrosourea ( MNU) .
METHODS: Thirty-six 7-week old Sprague-Dawley ( SD ) rats were intraperitoneally injected with MNU ( 60mg/kg ) and were put to death by dislocation of cervical vertebra 6, 12, 24h; 3, 7d after injection ( 6 per group) , respectively. As a control, six rats were injected with phosphate buffer saline (PBS) 5mL/kg and sacrificed on d3 after injection. The degree of photoreceptor apoptosis was detected by HE staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling ( TUNEL ) and transmission electron microscope ( TEM ) in the right eyes. The mRNA expressions of rhodopsin and recoverin were detected different time after injection by Western blot and immunohistochemical method in the left eyes.
RESULTS:The dissolution of photoreceptor nucleus and apoptosis body were first perceived at 12h by TEM; most of cells at outer nuclear layer were presented positive reaction. The apoptotic index reached peak ( 29. 7% ±2.3%) at 24h which was coincided with the observation of TEM. The results of immunohistochemistry displayed that rhodopsin and recoverin were on a declining curve with time extension. Furthermore, the results of Western blot indicated that rhodopsin had dramatic decline at 6h after injection (P<0. 05), and extremely significant difference comparing to control group after 12h ( P<0. 01 ); while recoverin dramatic declined at 12h, and extremely significant difference after 24h (P<0. 01).
CONCLUSION:60mg/kg MNU intraperitoneally injection one - time may specifically induce photoreceptor apoptosis, The mechanism of down - regulation of rhodopsin and recoverin may be related to the selected apoptosis of photoreceptors.

?AIM: To study the association of the single nucleotide polymorphism ( SNP) rs1157699 in the calcitonin receptor-like receptor ( CRLR ) gene with primary angle closure ( PAC) in a Han Chinese population.?METHODS: All samples, involved 232 PAC cases and 306 controls, were obtained from an epidemiologic survey conducted in Funing, Jiangsu Province, China. Genotyping were carried out by TaqMan-MGB probe using the real time quantitative polymerase chain reaction system to study the relationship between SNP of rs1157699 in CRLR gene and PAC.?RESULTS: The prevalence of CRLRrs1157699 genotype was 67.4%, 30.0%, 2.6% for CC, CT, TT in cases, and 71.3%, 27.0%, 1.7% in controls respectively.There was no difference between the two groups in the distribution of genotype and allele frequencies of rs1157699 (P>0.05).?CONCLUSION:Our results do not support a significant role for rs1157699 in CRLR with PAC.

OBJECTIVETo evaluate the therapeutic effect of Yinchenghao decoction (YCHD) and S-adenosy-L-methionine (SAM) in treating intrahepatic cholestasis of pregnancy (ICP) and improving prognosis of perinatal newborn babies.

METHODSSixty in-patients of ICP were randomly divided into two groups, the group A treated with YCHD and the group B treated with SAM. The symptom of itching and serum biochemical indexes, including glycocholic acid, bilirubin and transaminase, were observed after 3 weeks treatment, and the prognosis of perinatal newborn babies between the two groups was compared after delivery.

RESULTSAfter treatment, the symptom of itching, serum levels of glycocholic acid, bilirubin and transaminase improved significantly (P < 0.05) in both groups, and the prognosis of newborn in the two groups was similar (P > 0.05).

CONCLUSIONBoth YCHD and SAM could effectively treat ICP. The former is rather cheaper, so it is more feasible for spreading.

Adult ; Cholestasis, Intrahepatic ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Phytotherapy ; Pregnancy ; Pregnancy Complications ; drug therapy ; Pregnancy Outcome ; S-Adenosylmethionine ; therapeutic use

AIM: To discuss the clinical effect of 23-gauge pas plana vitrectomy ( PPV) for delay-onset endophthalmitis following cataract surgery.? METHODS: This retrospective chart review study included patients with delay-onset endophthalmitis that was present 6wk or more after cataract surgery, who underwent 23-gauge PPV from January 2010 to January 2016 at Renmin Hospital of Wuhan University. The diagnosis was made according to clinical symptoms and signs. The postoperative follow - up results were recorded, including anterior segment examinations, fundus examinations, and best-corrected visual acuity.?RESULTS:Totally 15 patients (15 eyes) were included. The average time between cataract surgery and their eye symptoms of endophthalmitis was 5. 13 ± 2. 41mo. The culture isolates revealed that 9 eyes ( 60％) were cultured positive, anaerobes in 4 eyes (44％), fungal infections in 3 eyes ( 33％) , aerobes in 2 eyes ( 22％) . The mean follow-up period was 12mo. The final visual outcomes were as follows:11 eyes ( 73％) achieved a better vision with PPV; 3 eyes ( 20％) achieved a better vision with PPV, total capsulectomy ( TC ) and IOL removal; 1 eye ( 7％) with PPV, silicone oil tamponade, total capsulectomy ( TC ) and IOL removal was enucleated because of uncontrolled inflammation.?CONCLUSION: The 23-gauge PPV is safe and effective for the management of delay - onset endophthalmitis following cataract surgery. Recurrence rate might be decreased with total capsulectomy and IOL removal.

Objective To investigate the correlation of single nucleotide poly morphisms (SNP) of silent information regulator 1 (SIRT1) gene with age-related cataract (ARC) in Jiangsu Har population.Methods Population-based case-control study was conducted in 720 cataract cases and 701 healthy controls,who were selected from the database of Funing County of Northern Jiangsu and Binhu District of Wuxi city of Southern Jiangsu.Five SNPs of SIRT1 gene (rs2236319,rs1885472,rs10997868,rs2273773 and rs4746720) were genotyped by Taq-Man RT-PCR methods.Results The genotype of 2 SNPs in SIRT1 (rs2236319 and rs1885472) was AA/AG/GG and CC/CG/GG respectively,and the genotype frequency in the controls was 40.08％,52.60％,7.32％ and 35.81％,35.23％,28.96％ respectively,while the frequency in ARCs was 36.94％,56.25％,6.81％ and 36.67％,32.92％,30.42％,respectively.The genotype of another 2 SNPs in SIRT1 (rs2273773 and rs4746720) was both CC/CT/TT,and the genotype frequency in the controls was 38.37％,54.78％,5.85％ and 74.75％,17.69％,7.56％ respectively,while the frequency in ARCs was 39.58％,54.17％,6.25％ and 71.81％,20.97％,7.22％,respectively.And there was no significant difference between the two groups (P ＞ 0.05).Conclusion All the five SNPs of SIRT1 gene are not associated with ARC in the Jiangsu Han population.

Phase 1 clinical trials is a key step for new drugs from basic experiments to human verification.Developed countries have formulated the corresponding laws , regulations and policies and guiding principles.Association The British Pharmaceutical Industry ( ABPI ) released a 2012 version of guidelines for phase 1 clinical trials.It provided professional guidances for some important problems in phase 1 clinical trials ( such as risk assessment , contracts and agreements , confidentiality , subjects , in-vestigational medicinal products and so on ).This paper makes a brief in-troduction of guiding principles for phase 1 clinical trials on the 2012 ver-sion of the ABPI.

AIM:To investigate the prevalence of age-related macular degeneration (ARMD) among people aged 50 years and above in Funing county,Jiangsu province.METHODS:Survey research.Random cluster sampling was used in selecting individuals aged ≥ 50 years in 30 clusters in Funing county.Proportions were compared by using the x2 test and the means compared by using the ttest.Logistic regression was used to detect possible factors of ARMD such as age and gender.RESULTS:A total of 6145 persons aged 50 years were enumerated and 5947 (96.78％) participants were received visual acuity test and eye examination.The prevalence of ARMD was 7.53％,with a total of 448 individuals (633 eyes).The prevalence of blindness and visual impairment for presenting visual acuity were 4.13％ and 11.96％,respectively.The prevalence of blindness and visual impairment for presenting visual acuity were 4.45％ and 7.79％,respectively.Older were significant risk factors of ARMD (OR=1.01,P=0.04).CONCLUSION:The prevalence of ARMD was higher among people aged 50 years and above in Funing county,Jiangsu province.ARMD is one of the leading cause of visual impairment.

Objective To investigate the prevalence of uncorrected refractive errors among urban population aged 50 years and above in Ftming county,Jiangsu province.Methods Survey research was conducted and randomly cluster sampling was used to select individuals aged ≥50 years for visual acuity test and eye examination in Funing county,Jiangsu province.The criteria of uncorrected refractive errors in this study was defined as an improvement of at least 0.2 log MAR (equivalent to 2 lines) in the best corrected visual acuity with the base vision ＜ 0.5 log MAR in daily life.The quantitative data were expressed as mean ± standard deviation,and t-test was used for comparison between groups,and while the count data were expressed as rate or composition ratio,and the x2 test was adopted for comparison between the groups.Logistic regression was used to examine the effect of possible factors (i.e.age and gender) on the prevalence of uncorrected refractive errors.Results A total of 6145 persons aged 50 years and above were enumerated and 5947 (96.8％) participants were examined,of whom 2388 had uncorrected refractive errors,with the prevalence of 40.2％.The prevalence of uncorrected refractive errors for myopia only,hyperopia only,astigmatism,and for hyperopia and astigmatism were 84.4％,84.2％,64.1％ and 100％,respectively.Moreover,the higher prevalence of uncorrected refractive errors presented in elderly person (OR =1.07,P ＜ 0.00l) and female (OR =1.38,P ＜ 0.001),and education was a protective factor for junior high school (OR =0.74,P =0.003) and high school (OR =0.55,P ＜ 0.001).Conclusion Uncorrected refractive errors presented high prevalence in rural population aged 50 years and above in Funing county,Jiangsu province,which are the leading cause of visual impairment.

Objective To examine the association of 18 single nucleotide polymorphisms (SNPs) in 4 DNA repair genes (BLM,WRN,ERCC6 and OGG1) with age-related cataract (ARC) in a Han Chinese population from Jiangsu Eye Study and to determine the possible functional consequence of the SNPs to DNA damage.Methods Together 18 SNPs in 4 DNA repair genes were genotyped in 789 ARC patients and 531 normal controls from Jiangsu Eye Study.The Comet assay was conducted to assess the extent of DNA damage in peripheral lymphocytes of the selected subjects.Results The results showed that WRN-rs11574311 was initially associated with ARC in general,cortical and mixed cataracts (P =0.003,OR =1.49;P =0.001,OR =1.68 and P ＜0.000 1,OR=2.08),BLM-rs1063147 with nuclear cataracts (P =0.03,OR =1.31),WRN-rs2725383 with cortical cataracts (P =0.01,OR =1.49),WRN-rs4733220 and WRN-rs2725338 with mixed cataracts (P =0.04,OR =0.74 and P =0.003,OR =0.60).However,after Bonferroni corrections,WRN-rs11574311 was still associated with cortical and mixed cataracts,as well as WRN-rs2725338 with mixed cataracts.The difference in DNA damage of peripheral lymphocytes with SNP types did not approach statistical significance among groups (all P ＞ 0.05).Conclusion WRN genes may have a vital role in ARC pathogenesis and exert a different action in ARC subtypes,which may be associated with different risk factors and mechanisms.