1.Changes of S-100B Protein in Umbilical Cord Blood of Asphyxial Neonates with Hypoxic-Ischemic Encephalopathy and Its Clinical Significance
Journal of Applied Clinical Pediatrics 2006;0(14):-
Objective To study clinical significance of S-100B protein in umbilical cord blood of asphyxial neonates with hypoxic-ischemic encephalopathy(HIE).Methods Forty-three cases of healthy infants were regarded as control group(group 1).Sixty cases of asphyxial neonates were divided into two groups(44 cases without HIE as group 2;another 16 cases with HIE as group 3).S-100B protein levels in umbilical cord blood of three groups were measured with enzyme-linked immunosorbent assay retrospectively. Results Levels of S-100B protein in group 1,group 2 and group 3 were(1.055?0.356) ?g/L,(1.572?0.533) ?g/L and(2.394?(0.943) ?g/L,) respectively.S-100B protein levels in umbilical cord blood of group 2 and group 3 were significantly higher than that of group 1(t=2.306,7.991 P
2.Chinese and Western medical treatment of varicocele caused infertility.
Chinese Journal of Integrated Traditional and Western Medicine 2007;27(11):971-971
Adult
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Drug Therapy
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methods
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Infertility, Male
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drug therapy
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etiology
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Male
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Phytotherapy
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methods
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Varicocele
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complications
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drug therapy
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surgery
3.Role of Serum S-100B Concentration in Diagnosis of Hypoxic-Ischemic Encephalopathy in Newborn Infants
huai-yan, WANG ; bin, YU ; chun-yuan, GAO ; jin-xiu, WANG ; xi-rong, GUO
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To explore the role of serum S-100B concentration of umbilical cord blood and blood on the 24 h after admission in the early diagnosis and development of newborn hypoxic-ischemic encephalopathy(HIE).Methods Forty-six HIE newborns(31 cases with mild HIE and 15 cases with moderate and severe HIE)were selected as HIE group,and 43 normal full-term newborns were selected as control group.The umbilical cord blood sample and blood sample were aquired on the 24 h after admission.The serum S-100B concentration was detected by enzyme-linked immunosorbent assay(ELISA)analysis.Results 1.There was no significant difference of serum S-100B concentration between the male sub-group and female sub-group of normal group and their birth weight had no significant relative to the serum S-100B concentration.2.The serum S-100B concentration of umbilical cord blood of control group and HIE group were(1.03?0.32)and(2.53?1.1)?g/L,respectively,there was significant difference between two groups(t'=8.848 P
4.Combined use of urinary bladder cancer antigen,hyaluronic acid and survivin for the detection of bladder cancer
Xiao-Yong PU ; Li-Quan HU ; Zhi-Ping WANG ; Yi-Rong CHEN ; Xing-Buan WANG ; Huai-Peng WANG ; Shi-Wen LI ; Xin-min ZHENG ; Zhong-jin YUE
Chinese Journal of Urology 2001;0(07):-
Objective To assess the diagnostic value of combined testing of urinary bladder cancer antigen(UBC),hyaluronic acid(HA)and survivin in the detection of bladder cancer.Methods This study included 64 bladder cancer patients and 20 urinary benign disease patients.The examinations of urine UBC by enzyme-linked immunosorbent assay(ELISA),HA by radioimmunology assay,survivin by RT-PCR and urine cytology were performed in them.Results The sensitivity of UBC(85.9%,55/64),HA (89.1%,57/64)and survivin(93.8%,60/64)was significantly higher than that of urine cytology (40.6%,P<0.01).The specificity of UBC,HA,survivin and urine cytology was 85.0%(17/20),80.0% (16/20),95.0%(19/20)and 95.0%(19/20),respectively;there was no significant difference among these 4 methods(P>0.05).The sensitivity of UBC,HA and survivin was also significantly higher than that of urine cytology in different histologic stages and grades(P<0.05).The sensitivity of UBC and survivin was not significantly different among different histologie stages and grades(P>0.05).With regard to HA test, the sensitivity in G_2 and G_3 groups was significantly higher than G_1 group(P<0.01),but there was no differ- ence between G_2 and G_3 groups(P>0.05);and no difference among different histologic stages(P>0.05). However,the sensitivity of cytology was improved with the higher grade of bladder cancer(P<0.01);there was no difference among histologic stages(P>0.05),By combined use of UBC,HA and survivin,both the sensitivity and specificity were 100%.Conclusions The study indicates that UBC,HA and survivin are better diagnostic markers for the early detection of urinary bladder cancer.These tests are simple,feasible and noninvasive with higher sensitivity and specificity.In addition,combined use of them can improve the diag- nostic sensitivity and specificity.
5.Background chloride currents in fetal human nasopharyngeal epithelial cells.
Xue-Rong SUN ; Li-Wei WANG ; Jian-Wen MAO ; Lin-Yan ZHU ; Si-Huai NIE ; Ping ZHONG ; Li-Xin CHEN
Acta Physiologica Sinica 2005;57(3):349-354
To characterize the background current in fetal human nasopharyngeal epithelial cells and clarify its relationship with volume activated Cl(-) currents (I(Cl,vol)), whole-cell patch clamp and cell imaging techniques were employed. Under isotonic conditions, a background current [(5.9+/-2.1) pA/pF at +80 mV, n=21] was detected. The current presented a weak outward rectification and a negligible time-dependent inactivation. The current-voltage relationship showed that the reversal potential of the background current [(-0.73+/-1.7) mV, n=21] was close to the calculated equilibrium potential for Cl(-)(-0.9 mV). Application of extracellular hypertonic stimulation (440 mOsmol/L) suppressed the current by (59.6+/-7.1)% and the inhibition was reversible after returned to isotonic conditions. Bathing the cells in hypotonic solution (160 mOsmol/L) induced a volume-sensitive Cl(-) current. The Cl(-) channel blockers, tamoxifen (20 micromol/L) and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) (100 micromol/L), inhibited the background current by (74.0+/-5.2)% (P<0.01, n=5) and (60.9+/-8.9)% (P<0.01, n=6) at +80 mV and increased basal cell volume by (107.7+/-2.9)% (P<0.01, n=25) and (104.4+/-2.4)% (P<0.01, n=19), respectively. The data indicate that Cl(-) current is an important component of the background current in fetal human nasopharyngeal epithelial cells. The background Cl(-) current is involved in volume activated Cl(-) current and basal cell volume regulation.
Cells, Cultured
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Chloride Channels
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antagonists & inhibitors
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physiology
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Electrophysiology
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Epithelial Cells
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cytology
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metabolism
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physiology
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Fetus
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Humans
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Nasopharynx
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cytology
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Nitrobenzoates
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pharmacology
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Patch-Clamp Techniques
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Tamoxifen
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pharmacology
6.Pharmacokinetics of bromotetrandrin (W198) in rats and beagle dogs.
Shu-huai XIAO ; Guang-li WEI ; Rong LU ; Chang-xiao LIU ; Feng-peng WANG
Acta Pharmaceutica Sinica 2004;39(4):301-304
AIMTo study the pharmacokinetics of bromotetrandrine (W198) in rats and beagle dogs.
METHODSThe concentrations of W198 in serum were determined using HPLC method with UV detection.
RESULTSThe pharmacokinetic parameters of W198 after single iv doses of W198 10, 20 and 40 mg x kg(-1) in rats were as follows: T1/2beta were 6.60, 7.36 and 6.77 h, AUC0-24 h were 3.797, 7.371 and 15.192 mg x h x L(-1), Vd were 7.14, 4.33 and 4.13 L x kg(-1), CL were 2.83, 2.60 and 2.71 L x (kg x h)(-1), respectively. The T1/2beta and AUCo-24 h of W198 after single im dose of W198 20 mg x kg(-1) in rats were 11.61 h and 12.646 mg x h x L(-1). The im bioavailability of W198 in rats was 56.9%. The T1/2beta, AUC0-24 h, Vd and CL of W198 after single iv dose of W198 5 mg x kg(-1) in beagle dogs were 11.72 h, 12.646 mg x h x L(-1), 0.70 L x kg(-1) and 0.46 L x (kg x h)(-1), respectively. The plasma protein binding ratio of W198 with human serum protein was 78.0%.
CONCLUSIONThe absorption of W198 in rats was of first order kinetics.
Alkaloids ; metabolism ; pharmacokinetics ; Animals ; Antineoplastic Agents ; metabolism ; pharmacokinetics ; Area Under Curve ; Benzylisoquinolines ; metabolism ; pharmacokinetics ; Biological Availability ; Blood Proteins ; metabolism ; Dogs ; Drugs, Chinese Herbal ; metabolism ; pharmacokinetics ; Female ; Humans ; Male ; Protein Binding ; Rats ; Rats, Wistar ; Species Specificity
7.Clinical observation on acupuncture combined with microorganism pharmaceutical preparations for treatment of irritable bowel syndrome of constipation type.
Ze-rong LONG ; Cun-hai YU ; Yu YANG ; Huai-ning WANG ; Xiao-xia CHI
Chinese Acupuncture & Moxibustion 2006;26(6):403-405
OBJECTIVETo explore the best program for treatment of irritable bowel syndrome (IBS) of constipation type.
METHODSNinety-five cases of IBS were randomly divided into 3 groups. Group A (n = 30) were treated by acupuncture combined with microorganism pharmaceutical preparations, group B (n = 35) by oral administration of medicine for loosening the bowel to relieve constipation plus microorganism pharmaceutical preparations, and group C (n = 30) by simple acupuncture.
RESULTSThe total effective rates were 90.0%, 77.2% and 66.7%, in the group A, B and C, respectively, with a very significant differences as the group A compared with those in the groups B, C (P < 0.01), and with no significant difference as the group B compared with that of the group C (P > 0. 05). The intestinal available bacteria, bilidobacteria and lactobacillus, increased and enteric bacilli decreased in varying degrees in the 3 groups.
CONCLUSIONAcupuncture combined with microorganism pharmaceutical preparations has a better therapeutic effect on irritable bowel syndrome of constipation type.
Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Constipation ; therapy ; Female ; Humans ; Intestines ; microbiology ; Irritable Bowel Syndrome ; microbiology ; therapy ; Male ; Probiotics ; therapeutic use
8.Quantitation of albumin in urine by HPLC and initial clinical application
Xiao-Fei CHEN ; Jian-Rong YANG ; Li-Ling CHEN ; Yao-Sheng XIE ; Huai-Kai WEN ; Xiao-Fang HUANG ; Yu-Min WANG ;
Chinese Journal of Laboratory Medicine 2001;0(01):-
Objective To measure albumin in urine by HPLC and conduct primary clinical application Methods Solvent gradient and appropriate wave length was optimized and performance of the HPLC method was evaluated.Urine albumin of 46 patients with diabetes was measured.Results In standard and urine,retention time of Alb was 13.1 min.The linear measuring range extends to 1 820 mg/L.The lower limit of measurment for Alb was 4.2 mg/L.The intra-assay CV and the inter-assay CV were 3.36%,4.12% and 1.93%,1.97% at 24.5 mg/L and 546.9 mg/L of Alb respectively.Analytical recovery rate were 96.3%,98.2% and 97.5%.Microalbuminuria rate was 54.3% by HPLC,26.1% by immunoassay in 46 patients with diabetes.Conclusions Measurement of Alb in urine by HPLC is feasible as routine method until quantifying urinary total Alb conveniently.HPLC is the same to suit research for diabetic nephropathy and so on.
9.Discussion on innovative teaching management mode in large scale general hospital
ling Ling WANG ; min Huai GU ; xiu Yu LIU ; rong Zhao SHI
Journal of Medical Postgraduates 2017;30(12):1313-1317
Teaching management is an important link in hospital training of high-quality medical talents and an indispensable part of the overall construction of hospitals.Aiming at the confusion of the teaching work and combined the teaching management practice of the hospital,we put forward "54321" teaching management model,introduced the operation and effectiveness of this model.In conclusion,we provide a way to further strengthen the teaching management for general hospitals.
10.Construction of Nonenzymatic Glucose Sensor Based on One Step Preparation of Poly(3,4-ethylenedioxythiophene) Graphene Composite Materials by Pulse Potentiostatic Method
Jing Jing LI ; Hui LI ; Ping Li JIA ; Na Rong MA ; Li Wen JIA ; Sheng Huai WANG
Chinese Journal of Analytical Chemistry 2017;45(12):2004-2010
The Poly(3,4-ethylenedioxythiophene) graphene ( PEDOT-rGO) composite film was prepared by one step pulse potentiostatic method, based on the π-π* interaction and the polymerization reaction of hydrogen bond between the graphene ( rGO) and the aromatic ring of 3,4-ethylenedioxythiophene ( EDOT) monomer. Then the nickel nanoparticles ( NiNPs ) were electrodeposited on composite film to obtain a composite film-modified glassy carbon electrode ( NiNPs/PEDOT-rGO/GCE ) . The catalytic performance of NiNPs/PEDOT-rGO/GCE towards the oxidation of glucose was studied by electrochemical method. The experimental results showed that the NiNPs/PEDOT-rGO/GCE could be used as a nonenzyematic sensor for detection of glucose. This method had high stability, good selectivity, wide linear range ( 2 μmol/L-58 mmol/L) and low detection limit (0. 7 μmol/L), realizing the rapid and sensitive detection of glucose.