1.Effects of long-term excessive Iodine intake on the maternal-fetal thyroid hormone metabolism in mice
Xue-Feng YANG ; Jian XU ; Huai-Lan GUO ; Xiao-Hui HOU ; Xiao-Yi CHEN ; Xiu-Fa SUN
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
Long-term excessive iodine intake resulted in an increased TT_4 level and a decreased TT_3 level in maternal serum,meanwhile,hepatic and renal type 1 deiodinase activity decreased dose-dependently.A significant reduction in type 2 deiodinase ( D2 ) activity of 12.5 d placenta was found in 3.0 mg/L or above groups.For 19.5 d uterus,D2 activity decreased and type 3 deiodinase activity increased.The results suggest that excessive iodine has an effect on the embryonic development by regulating maternal-fetal thyroid hormone metabolism.
2.Expression of glypican-3, hepatocyte antigen, alpha-fetoprotein, CD34 and CD10 in hepatocellular carcinoma: a clinicopathologic analysis of 375 cases.
Jing-li DU ; Yu-lan WANG ; Huai-yin SHI ; Ai-tao GUO ; Li-xin WEI
Chinese Journal of Pathology 2012;41(5):309-313
OBJECTIVETo explore prognostic factors and the expression of glypican-3, hepatocyte antigen (HEP), alpha-fetoprotein (AFP), CD34 and CD10 in hepatocellular carcinoma (HCC) and their prognostic value.
METHODSClinicopathologic data were analyzed in 375 cases of HCC, in which 80 cases with follow-up were examined by immunohistochemical staining to detect the expression of glypican-3, HEP, AFP, CD34 and CD10 proteins. The relationship between the proteins expression and clinicopathologic features was also evaluated.
RESULTSTumor number (P = 0.000), tumor size (P = 0.025), tumor differentiation (P = 0.001) and vessel invasion (P = 0.000) were closely related to prognosis of HCC patients; the expression of glypican-3 (66/80,82.5%; P = 0.002), HEP (64/80,80.0%; P = 0.021), AFP (38/80,47.5%; P = 0.014) and CD10 (28/80,35.0%; P = 0.002) was significantly related to tumor differentiation; that of glypican-3 was significantly correlated with tumor number and presence of satellite nodules (P = 0.028) and that of AFP and CD10 was significantly correlated with portal vein thrombi (P = 0.000, P = 0.010). On Kaplan-Meier regression analysis, both low expression of HEP and high expression of AFP were closely related to poor prognosis.
CONCLUSIONSTumor number, size, differentiation and vessel invasion were important factors affecting the prognosis of patients with HCC. HEP and AFP have prognostic significance in HCC.
Antigens ; metabolism ; Antigens, CD34 ; metabolism ; Biomarkers, Tumor ; metabolism ; Carcinoma, Hepatocellular ; metabolism ; pathology ; surgery ; Cell Differentiation ; Female ; Follow-Up Studies ; Glypicans ; metabolism ; Hepatocytes ; immunology ; Humans ; Liver Neoplasms ; metabolism ; pathology ; surgery ; Male ; Neprilysin ; metabolism ; Portal Vein ; pathology ; Prognosis ; Survival Rate ; Tumor Burden ; Venous Thrombosis ; etiology ; pathology ; alpha-Fetoproteins ; metabolism
3.Toll like receptor 9 expression and functions of plasmacytoid dendritic cells in the peripheral blood of patients with chronic hepatitis B virus infection
Ni-Na JIA ; Qing XIE ; Bao-Yan AN ; Lan-Yi LIN ; Huai-Cheng SHEN ; Hui WANG ; Xia-Qiu ZHOU ; Hong YU ; Qing GUO ;
Chinese Journal of Infectious Diseases 2007;0(12):-
Objective To investigate the expression of Toll-like receptor(TLR) 9 of circulating plasmacytoid dendritic cell (pDC) and analyze the frequency and interferon (IFN)-?production of circulating pDC during hepatitis B virus(HBV) infection.Methods Peripheral blood was collected from 69 HBV-infected patients,including 14 cases of asymptomatie HBV infection,30 cases of chro- nic hepatitis B(CHB),25 cases of HBV-related liver cirrhosis,and 21 healthy blood donors as con- trols.Flow cytometry was used to analyze the frequency of circulating pDC and the mean fluorescence intensity(MFI) of TLR9.Fresh peripheral blood mononuclear cells(PBMC) were stimulated by CpG ODN 2216 for 24 h in vitro.IFN-?in the supernatant was measured using enzyme-linked immunosorbent assay(ELISA) to analyze the frequency and IFN-?production of pDC during HBV in- fection.Data were analyzed with SPSS 13.0 for windows.Results Compared with healthy controls (62.6?10.7),the MFI of TLR9 of patients with asymptomatic HBV infection,those of CHB and HBV-related cirrhosis were significantly reduced (P
4.Clinicopathologic features of hepatocellular carcinoma: an analysis of 185 cases.
Yu-lan WANG ; Jing-li DU ; Huai-yin SHI ; Ai-tao GUO ; Li-xin WEI
Chinese Journal of Oncology 2010;32(8):609-613
OBJECTIVETo analyze the clinicopathologic features and prognostic factors of hepatocellular carcinoma.
METHODSClinicopathological data of 185 cases of hepatocellular carcinoma treated in our hospital between 2000 and 2005 were collected and their follow up information was obtained. The clinicopathological features and prognostic factors were analyzed by Kaplan-Meier and multivariate Cox regression analysis.
RESULTSThe 185 patients had a median age of 51.0 ± 11.0 (range, 19 - 72) years. The apparent peak incidence age was 40 to 60 years old, and the ratio of male to female was 10.6:1; the 3- and 5-year postoperational survival rates were 52.0% and 38.0%; respectively. The tumour numbers (P = 0.000), tumor size (P = 0.025), histological pattern (P = 0.000), nuclear features (P = 0.000), differentiation (P = 0.001) and vascular invasion (P = 0.000) were significantly correlated with prognosis. The postoperational survival times of patients with thin trabeculae pattern, compact pattern and pseudoglandular pattern were significantly longer than that of thick trabeculae, scirrhous pattern, and solid pattern (P ≤ 0.009). The postoperational survival time of patients with nuclear features grade 1 and 2 was significantly longer than that of grade 3 and 4 (P = 0.000). Multivariate Cox regression analysis showed that the tumor number (P = 0.001), tumor size (P = 0.042), nuclear features (P = 0.023) and vascular invasion (P = 0.000) were independent prognostic factors.
CONCLUSIONThe postoperational survival rate of HCC patients is low. The tumor size, tumor number, differentiation and vascular invasion are major prognostic factors of hepatocellular carcinoma, The higher is the tumor number, tumor size, degree of differentiation and presence of vascular invasion, the higher risk of mortality is.
Adult ; Aged ; Carcinoma, Hepatocellular ; blood ; complications ; pathology ; surgery ; Cell Differentiation ; Cell Nucleus ; pathology ; Female ; Follow-Up Studies ; Hepatitis B Surface Antigens ; blood ; Humans ; Liver Cirrhosis ; complications ; Liver Neoplasms ; blood ; complications ; pathology ; surgery ; Male ; Middle Aged ; Neoplasm Invasiveness ; Neoplastic Cells, Circulating ; Proportional Hazards Models ; Survival Rate ; Tumor Burden ; Young Adult
5.Expression of Toll-like receptor 3 on peripheral blood dendritic cells in HBeAg positive patients with chronic hepatitis B.
Bao-yan AN ; Qing XIE ; Lan-yi LIN ; Huai-cheng SHEN ; Ni-na JIA ; Hui WANG ; Si-min GUO ; Hong YU ; Qing GUO
Chinese Journal of Hepatology 2007;15(10):729-733
OBJECTIVETo elucidate the roles of Toll-like receptor 3 (TLR3) on dendritic cells (DCs) in HBV infection.
METHODSPeripheral blood mononuclear cells (PBMCs) were isolated from 48 healthy volunteers (HV) and 50 chronically HBV-infected patients (CH). DCs were induced and proliferated in a culture medium with rhGM-CSF and rhIL-4. We stimulated DCs with poly I:C and then TLR3, HLA-DR, and CD86, and CD1a expressions were examined by flow cytometry at 0 h, 12 h, 24 h and 48 h. The mRNA expressions of TLR3 were quantified by real-time PCR.
RESULTSTLR3 expression on DCs before the poly I:C stimulation and afterwards on the 12 h, 24 h, and 48 h were 69.2%+/-20.4%, 76.0%+/-18.6%, 78.2%+/-19.5% and 85.5%+/-6.9% respectively in the CH group, and 70.8%+/-11.2%, 67.5%+/-20.9%, 86.3%+/-14.7%, 68.6%+/-16.9% in the HV group. The expressions of TLR3 were up-regulated significantly at 24 h and 48 h after stimulation with poly I:C in the HV group, and in the CH group they were not significantly increased at 24 h but obviously increased at 48 h. The mRNA expressions of TLR3 increased significantly at 12 h in the HV groups, and at 48 h in CH group. The rate of CD86 expressions increased after poly I:C stimulation, and the increased rates were 12.6%+/-9.8%, 23.8%+/-20.0%, 20.7%+/-14.3% in the CH group, and 31.0%+/-25.0%, 43.4%+/-24.7%, 44.6%+/-25.5% in the HV group at 12 h, 24 h and 48 h after poly I:C stimulation. There was a marked increase of the expression level of CD86 in the HV group. In contrast, the level was only slightly increased in the CH group (31.0% vs 12.6%). The differences between the two groups were significant at 24 h and 48 h. No significant differences were detected in HLA-DR and CD1a between the two groups.
CONCLUSIONSThe increase of expression level of TLR3 is slower in the CH group than that in the HV group. A marked increase of the expression level of CD86 is observed in the HV group. Our results suggest that abnormal expression of TLR3 and CD86 may relate to the persistence of HBV infection.
Adult ; B7-2 Antigen ; metabolism ; Dendritic Cells ; immunology ; metabolism ; Female ; Hepatitis B e Antigens ; blood ; Hepatitis B, Chronic ; blood ; Humans ; Male ; Middle Aged ; Toll-Like Receptor 3 ; metabolism ; Young Adult
6.Alternating magnetic field damages the reproductive function of murine testes.
Xing-wu CAO ; Tian-de ZHAO ; Chuan-hang WANG ; Qiang ZHOU ; Lan-qun LI ; Huai-guo YAO ; Sheng-Qiang ZHANG ; Jing-tian TANG ; Wei WEI
National Journal of Andrology 2009;15(6):530-533
OBJECTIVETo explore the relationship between physical and biological effects of alternating magnetic field and study the influence of the magnetic field on the reproductive function of murine testes.
METHODSThirty ICR mice were randomized into 5 groups: normal control, X-ray radiation, weak magnetic field (1000 Hz), 1 h strong magnetic field and 2 h strong magnetic field (2000 Hz). The mice were sacrificed at 7 days after the exposure for the analysis of testicular sperm motility, observation of histopathological changes in the testis by HE staining and evaluation of the changes by modified Johnsen grade criteria.
RESULTSThe rates of sperm motility were (42.37 +/- 10.24)% in the normal control group, (39.00 +/- 12.35)% in the X-ray radiation group, (36.00 +/- 17.28)% in the weak magnetic field group, (10.72 +/- 5.67)% in the 1 h strong magnetic field group and (4.44 +/- 2.87)% in the 2 h strong magnetic field group, respectively. Johnsen's scores decreased and the testis damage increased in a dose- and time-dependent manner.
CONCLUSIONMagnetic field, either strong or weak, may damage the testis function by inducing injury to seminiferous tubules and Leydig cells, thickening of the basal membrane, derangement, exfoliation, massive apoptosis and necrosis of spermatogenic cells in the lumen, situation of the epididymis, and consequently the absence of sperm.
Animals ; Electromagnetic Fields ; adverse effects ; Leydig Cells ; pathology ; Male ; Mice ; Mice, Inbred ICR ; Sperm Motility ; Testis ; cytology ; pathology ; radiation effects
7.Effect of selenium supplementation on activity and mRNA expression of type 1 deiodinase in mice with excessive iodine intake.
Xue-Feng YANG ; Xiao-Hui HOU ; Jian XU ; Huai-Lan GUO ; Chen-Jiang YINQ ; Xiao-Yi CHEN ; Xiu-Fa SUN
Biomedical and Environmental Sciences 2006;19(4):302-308
OBJECTIVETo investigate the effect of selenium supplementation on the selenium status and selenoenzyme, especially the activity and mRNA expression of type 1 deiodinase (D1) in mice with excessive iodine (EI) intake and to explore the mechanism of selenium intervention on iodine-induced abnormities.
METHODSWeanling female BALB/c mice were given tap water or 3 mg/L of iodine or supplemented with 0.5 mg/L or 1.0 mg/L of selenium in the presence of excessive iodine for 5 months. Selenium status, thyroid hormone level, hepatic and renal D1 activity and mRNA expression were examined.
RESULTSExcessive iodine intake significantly decreased the selenium concentration in urine and liver, and the activity of glutathione peroxidase (GSH-Px) in liver. Meanwhile, serum total T4 (TT4) increased while serum total T3 (TT3) decreased. Hepatic D1 enzyme activity and mRNA expression were reduced by 33% and 86%, respectively. Renal D1 enzyme activity and mRNA were reduced by 30% and 55%, respectively. Selenium supplementation obviously increased selenium concentration, activity of GSH-Px and Dl as well as mRNA expression of D1. However, increasing the supplementation of Se from 0.5 to 1.0 mg/L did not further increase selenoenzyme activity and expression.
CONCLUSIONRelative selenium deficiency caused by excessive iodine plays an essential role in the mechanism of iodine-induced abnormalities. An appropriate dose of selenium supplementation exercises a beneficial intervention.
Animals ; Antioxidants ; pharmacology ; Creatinine ; metabolism ; urine ; Dietary Supplements ; Female ; Iodide Peroxidase ; genetics ; metabolism ; Iodine ; toxicity ; urine ; Kidney ; metabolism ; Liver ; metabolism ; Mice ; Mice, Inbred BALB C ; RNA, Messenger ; metabolism ; Selenium ; pharmacology ; urine ; Thyroxine ; blood ; Triiodothyronine ; blood
8.Correlation between the expression of JARID1B/KDM5B in breast cancer tissues and the circulating tumor cells
Li-Hua ZHAO ; Jia WEI ; Ying-Li HUAI ; Xiao-Jing ZHANG ; Chao ZHANG ; Qiu-Lan LIN ; Ke-Jie WANG ; Hong-Yan YAN ; Jia-Liang GUO ; Qiu-Ying LI
Basic & Clinical Medicine 2018;38(4):512-515
Objective To investigate the relation between expression of JARID1B/KDM5B in invasive breast cancer tissue and circulating tumor cells of invasive breast patients. Methods The S-P immunohistochemical method was used to observe the expression of JARID1B/KDM5B in lesions.imFISH method was used to detect circulation tumor cells. Results Twenty-seven cases of patients showed CTC positive(the number of CTC was more than or equal to 2) in 35 invasive breast cancer cases,The results of JARID1B/KDM5B expression were as follows:(-) (n=0) and (+) in 1 case,(++) in 3 cases,(+++) in 23 cases,8 cases of negative cases (the number of CTC < 2), JARID1B/KDM5B immune group of results:(-) (n=2),(+) in 2 cases,(++) in 1 case,(+++) in 3 cases, The positive relation was found in expression of JARID1B/KDM5B and circulating tumor cells(P<0.05). Conclu-sions JARID1B/KDM5B positive expression in tumor tissues has a certain significance in predicting the recurrence and metastasis of tumor.
9.Research on reserves estimation method of wild medicinal plants resource for generous species based on spatial information technology.
Wei-Feng MA ; Xiao-Bo ZHANG ; Lan-Ping GUO ; Ben-Gang ZHANG ; Run-Huai ZHAO ; Lu-Qi HUANG ; Shou-Dong ZHU ; Yu QIAO
China Journal of Chinese Materia Medica 2013;38(8):1130-1133
Traditional Chinese medicinal resource survey method is time-consuming, strenuous, and having great human influence, the precision is not high enough. This paper, by using spatial information technology, carries on spatial sampling survey for wild medicinal plants resource for generous species to arrange the quadrat scientifically and estimate the suitable area, reserve precisely of medicinal plants. It not only improves the survey precision, but reduces the workload of field survey and provides scientific basis for survey method of pilot work on the fourth national traditional Chinese medicinal resource census.
Biodiversity
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Conservation of Natural Resources
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Ecosystem
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Humans
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Plants, Medicinal
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Spatial Analysis
10.Formulation of technical specification for national survey of Chinese materia medica resources.
Lan-Ping GUO ; Jian-Wei LU ; Xiao-Bo ZHANG ; Run-Huai ZHAO ; Ben-Gang ZHANG ; Li-Ying SUN ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2013;38(7):937-942
According to the process of the technical specification (TS) design for the fourth national survey of the Chinese materia medica resources (CMMR), we analyzed the assignment and objectives of the national survey and pointed out that the differences about CMMR management around China, the distribution of CMMR and their habitat, the economic and technological level, and even enthusiasm and initiative of the staff, etc. are the most difficult points for TS design. And we adopt the principle of combination of the mandatory and flexibility in TS design. We fixed the key points which would affect the quality of national survey first, then proposed the framework of TS which including 3 parts of organization and 11 parts of technique itself. The framework will serve and lead the TS preparation, which will not only provide an action standard to the national survey but will also have a profound influence to the popularization and application of the survey technology of CMMR. [Key words]
Chemistry, Pharmaceutical
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legislation & jurisprudence
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manpower
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methods
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standards
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China
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Conservation of Natural Resources
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Data Collection
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Drugs, Chinese Herbal
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chemistry
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standards
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Materia Medica
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chemistry
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standards
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Quality Control