Objective To establish a new effective method by using real-time polymerase chain reaction (PCR) to detect single nucleotide polymorphism (SNP) typing for rapid identifying apolipoprotein E alleles.Methods To determine alleles of human apolipoprotein E genetic polymorphism at Cys112Arg locus was detected by PCR melting curve analysis with fluorophore SYBR Green I. In order to increase the speciality of SNP assays, high fidelity Taq polymerase was used. The reliability of SNP typing was validated by comparison with the results of direct DNA sequencing.Results Each sample was determined by double tubes, and two melting curves were analysis. As compared the Tm value of samples with the Tm of standard substance, the apoE genotype of samples was determined. The apoE genotype of 30 samples were E3/3 (27/30) and E3/4 (3/30) respectively, which was accordant with the results of PCR-RFLP and DNA sequencing.Conclusion The presented allelic assay was specific, easy to operate and applicable for discrimination of apolipoprotein E genotyping of human blood.

Objective To search the clinical methods for identification of syphilis cerebrovascular infarction from atherosclerotic cerebrovascular infarction. Methods The clinical manifestations, laboratory examination results, MRI results and DSA characteristics of 20 patients with cerebrovascular infarction (syphilis group) and 80 patients with atherosclerotic cerebrovascular infarction (atherosclerosis group), admitted to our hospital from February 2008 to August 2014, were retrospectively analyzed and compared. Results As compared with patients of the atherosclerosis group, the patients of syphilis group had significantly younger onset age ([47.4±11.3] y vs. [62.7±11.0]y), higher male proportion (9:1 vs. 4:1), higher proportion of farmers and individual workers (70%vs. 21.2%, P<0.05). Dizziness (35%) and headache (20%) were more common as the first symptom, and memory loss (15%) and walking instability (15%) were more common as first symptoms in patients of syphilis group, while limb weakness (75%) and unclear speech (35%) were more common as first symptoms in patients of atherosclerosis group; significant differences were noted between the two groups (P<0.05). In MRI, lesions in lobes of the brain (45.0%) were more common in syphilis group, and lesions in the basal ganglia were (67.5%)more common in atherosclerosis group; significant difference was noted between the two groups (P<0.05);lesions involved more than two sites (38.8%) or even three sites lesions (45.0%) were more common in patients of syphilis group. In DSA, middle cerebral artery stenosis (65%), internal carotid artery stenosis (38.8%) were more frequently found in patients of syphilis group, and more than two (75.0%) or even three (55.0%) vascular involvements were more common in syphilis group;significant difference was noted between the two groups (P<0.05). Conclusion The clinical features and imaging features of the syphilis cerebrovascular infarction are different from atherosclerotic cerebrovascular infarction; DSA is important in the diagnosis and treatment of syphilis cerebrovascular infarction.