Objective To study the protective effect of propofol on post-hypoxic injury of car-diomyocytes induced during hyperglycemia.Methods Rat primary H9C2 cardiomyocytes were cul-tured and the model of hypoxia-reoxygenation (HR)was constructed.The cells were assigned to the following groups:normal control group (group NC),high glucose group (group HG)and HR under high glucose group (group GR),and propofol treated groups in which cells were treated with various concentrations (12.5,25,50,and 100 μmol/L)of propofol (i.e.,groups P12.5,P25,P50 and P100)and the solvent DMSO group (D100 group,DMSO at 100 μmol/L)respectively.(CK-MB), cardiac troponin I (cTnI),lactate dehydrogenase (LDH),mitochondrial activity,intracellular ATP content and intracellular oxidation of the cells were compared.Results Compared with the groups NC and HG,the cell viability was significantly decreased,the concentration of LDH and MDA,relative concentration of CK-MB and cTnI increased significantly,and the activity of T-SOD,mitochondria and the relative concentration of ATP decreased significantly in the other groups (P<0.05).Com-pared with the group GR,the cell activity increased significantly,the concentration of LDH and MDA ,relative concentration of CK-MB and cTnI were Significantly decreased,and the activity T-SOD, mitochondrial and relative concentration of ATP increased significantly in the groups P12.5,P25 and P50 (P<0.05).Compared with the group P25,the cell viability decreased significantly,the concen-trations of LDH,MDA,relative concentrations of CK-MB and cTnI increased significantly,and the activity of T-SOD and mitochondria and relative concentration of ATP were significantly reduced in the groups P50,P100 and D100 (P<0.05).Conclusion Propofol can reduce the damage of myocar-dial cells by reducing the damage of mitochondria.