Objective To discuss the clinical impact of laparoscopic surgery combined with GnRH-a for endometriosis infertility. Methods From June 2013 to June 2014, 88 endometriosis infertility patients were selected and divided into study group and the control group by the random number table method. The patients in study group were treated with laparoscopic surgery combined with GnRH-a. The patients in control group were treated with only laparoscopic surgery. The clinical efficacy, ovarian function and pregnancy outcomes of the patients in two groups were compared and analyzed. Results Compared with control group, the patients in study group, the clinical treatment for endometriosis achieved complete remission rate was 65.91 % (29/44) and total effective rate was 93.18 % (41/44), there were increased significantly, and the recurrence rate was 2.27 % (1/44), there was decreased significantly, after statistical analysis, the differences were significant statistically (P < 0.05). Compared with control group, the patients in study group after treatment, the level of E2 was (80.96 ± 17.65) ng/L, there were increased significantly, while the level of LH was (10.94 ± 3.20) IU/L and FSH was (6.24 ± 1.38) IU/L, there were decreased significantly, after statistical analysis, the differences were significant statistically (P < 0.05). Compared with control group, the patients in study group, the pregnancy rate was 47.73 % (21/44) and the living rate was 45.45 % (20/44), there were increased significantly, while the not pregnancy rate was 52.27 % (23/44), there was decreased significantly, after statistical analysis, the differences were significant statistically (P < 0.05). Conclusions Laparoscopic surgery combined with GnRH-a could improve the clinical efficacy of endometriosis infertility patients effectively, and further improve the ovarian function of patients, improve the pregnancy rate significantly.

Background and Objectives: Mesenchymal stem cells (MSCs) have the multipotent capacity to differentiate into multiple tissue lineages as well as to self-renew, which is the main origin of adipocytes. IL6/IL6R pathway exerts a significant role in tissue regeneration and cell differentiation. Whereas, the underlying mechanism between IL6/IL6R pathway and MSCs adipogenesis differentiation remains elusive. Methods: MSCs from healthy donors were cultured in adipogenesis differentiation medium for 0∼14 days, during which their adipogenesis differentiation degree was evaluated by Oil Red O staining. The expression of IL6R was detected in MSCs during adipogenesis differentiation. Knockdown and overexpression of IL6R were respectively performed using siRNA and lentivirus to investigate its effect on MSCs adipogenesis differentiation. The adipogenesis marker genes expression and MAPK pathway activation were detected by Western blotting. The role of P38 pathway in the adipogenesis differentiation of MSCs was determined using the specific inhibitor SB203580. Results: The expression of IL6 and IL6R increased during adipogenesis differentiation in MSCs, which were positively correlated with Oil Red O quantification result. Knockdown and overexpression experiments demonstrated a positive correlation between the expressions of IL6R and MSCs adipogenesis differentiation, accompanied by same trend of P38 phosphorylation. Besides, the specific P38 inhibitor SB203580 markedly inhibited the adipogenesis differentiation potential of MSCs. Conclusions This study reveals IL6R facilitates the adiogenesis differentiation of MSCs via activating P38 pathway.