Influenza viruses are global epidemic and diversely difficult to distinguish,which threaten human’s survival and development very much.In recentyears,the frequent outbreaks of influenza prompt the rapid development of Influenza virus detection.Compare with the traditional isolated culture and immunological detection,molecular diagnostic technology is of high detection speed,high sensitivity and specificity,that gradually play an important role in the current Influenza virus de-tection.In order to provide a theoretical basis for the rapid diagnosis of Influenza virus in the clinic,the article summarize the update progress of molecular biology and diagnostic techniques of Influenza viruses.

Objective To evaluate the clinical practicability of cefoxitin disk diffusion method in detecting methicillin-resistant Staphylococci.Methods 163 strains of clinically isolated Staphylococci were tested.The cefoxitin,oxacillin disk diffusion test and PCR amplification of mecA gene were performed simultaneously.The method of PCR was used as the golden standard.Results 163 strains of clinically isolated Staphylococci were tested by PCR.The positive ratio of mecA gene was 81.0%,The ocurrence rate of methicillin-resistant S.aureus(MRSA) and (methicillin-resistant coagulase-negative Staphylococci(MRCNS) was 74.1% and 83.8%,respectively.The cefoxitin,oxacillin disk diffusion and PCR were high consistency in detecting mecA gene.Both sensitivity and specificity were 100%,But cefoxitin disk diffusion was better for methicillin-resistant coagulase-negative Staphylococci.The sensitivity was 100% and 96.0%,the specificity was 94.3% and 88.0%,respectively.Conclusion Cefoxitin disk diffusion has high sensitivity in detecting methicillin-resistant Staphylococci,but for methicillin-resistant coagulase-negative Staphylococci which mecA gene is negative,5% strain may be reported as MRCNS by mistake.Since cefoxitin disk diffusion method is simple,and has higer sensitivity and specificity than oxacillin disk diffusion method in detecting MRS,the result has good correlation with PCR in detecting mecA gene.It may be applied in clinical microbiology laboratory.

Objective Use the automatic microbiological analysis system which for the streptococci (ATB rapid 32 Strep) to identify streptococci, and compare the examination speed and accuracy with the internationally recognized bacterium identification golden method API 20 Strep (streptococci identification biochemistry system) to enable the clinical microorganism room use ATB rapid 32 Strep identification system correctly, and provide the etiology basis to clinical promptly.Methods Isolate the bacterium from patient blood, cerebrospinal fluid, pig heart, pork and so on, then separate the streptococci in form to the blood plate, lay them in carbon dioxide incubator, after 35 ℃ 24 hours. Use ATB rapid 32 Strep,API 20 Strep and the traditional manual method to identify 18 strains of bacterias and carry on the medicine sensitive experiment.Results ATB rapid 32 Strep identified 16 strains as streptococcus suis II, 2 strains as streptococcus bovis II; API 20 Strep identified 17 strains as streptococcus suis II, 1 strain can not be identified; the tradition manually method identified all of the 18 strains as streptococcus group D . These 18 strains all sensitive to the Chloramphenicol , clindamycin, erythromycin, levofloxacin, penicillin, quinupristin/dalfopristin, cefotaxime, vancomycin and sulfafurazole , and resistant to the tetracyclin.Conclusion ATB rapid 32 Strep can identify the streptococcus suis in 4 hours , and the coincidence rate with API 20 Strep is 94.4%.So ATB rapid 32 Strep is one kind of system which could identify bacterium simply,quickly,accurately,and provide infectious disease etiology information for clinical immediately, satisfy the requirement of clinician and the microorganism examines.

Objective To assess the efficacy of Danggui Yinzi versus western medicine for treating chronic urticaria.Methods CNKI,VIP,Wanfang data,EMbase,the citation databases of PubMed,web of science updated to August 2012 were electronically searched.Randomized controlled trials (RCTs) and Clinical controlled trial (CCTs) on Danggui Yinzi and western medicine for chronic urticaria were identified.The quality of included RCTs and CCTs was assessed,and the meta-analysis was conducted with Revman5.1 software.Funnel-plot was used to identify the publication bias and performed sensitivity analyses.Results 13 trails involving 1042 patients were included.The qualities of all these trails were relatively low.There was no trail set double blinding and allocation concealment.Meta-analysis indicated that compared with western medicine,the effective rate of Danggui Yinzi was superior to that of western medicine[OR=2.53,95％CI(1.92,3.34)].The funnel-plot demonstrated that there was publication bias.Conclusion The current evidence showed that Danggui Yinzi was superior to western medicine in efficacy,but we need more multicenter,randomized,double-blind trails to support it.

Objective To explore protective effect of heparin on renal of children with Henoch-Schonlein purpura(HSP).Methods Two hundred and fifteen cases with HSP were divided into heparin-treated group(110 cases,male 64,female 46) and control group (105 cases,male 61,female 44).Serum IL-6 and IL-8 levels were examined by using radioimmunoassay respectively.These patients were followed up for more than 6 months.Results 1.The level of IL-6,IL-8 did not show significant difference between heparin-treated group and control group before treatment.The serum concentrations of IL-6,IL-8 in heparin-treated group were obviously lower than those in control group,after treatment,there was significantly different[IL-6(116.50?19.45)vs(123.88?22.55)ng /L,t=2.573 P=0.011;IL-8(0.161?0.043)vs(0.173?0.048)?g/L,t=2.024 P=0.044].2.During more than 6 months follow-up there were 26 cases suffering from purpura nephritis in heparin-treated group, the morbidity was 25%; the morbidity in control group was 39.8%,the morbidity in treatment group was significant lower than that in control group(?2=5.061 P=0.024).The time of renal injury was significantly delay in treatment group(35.0?13.2)d compared with that of control group (26.0?12.1)d(t=2.659 P=0.010).Conclusions The serum IL-6 and IL-8 concentrations in HSP obviously decrease after heparin treatment.Heparin therapy can decrease the morbidity of renal injury in children with HSP and delay the time of renal injury effectively.

Antitussive Agents ; poisoning ; Child, Preschool ; Dextromethorphan ; poisoning ; Humans ; Male

Biochemistry is an important basic medical course, and it is also a difficult course.In order to improve the comprehensive ability of seven-year program clinical medical students in Capital Medical University, teaching reform of Biochemistry was carried out.Teaching content was redesigned to strengthen the learning of basic knowledge of Biochemistry and to increase the depth of teaching content, while expanding the content by introducing new achievements and new technology to keep up with the forefront in the development of Biochemistry.Various methods such as the comparative method, case teaching and so on, were used flexibly in the teaching practice in order to improve teaching effects.The experiments carried out in the course were also redesigned and integrated, some distinctive experiments such as designing experiments, comprehensive experiments and innovative experiments were opened to improve students' hands-on ability and to cultivate students' scientific thinking.

In recent years dendritic cell tumor vaccine has been of great interest in medical research.In this paper,the author introduces the theory of immunotherapy on the basis of dendritic cell the development of the research in dendritic cell tumor vaccine and the clinical effect.The conclusion is that the clinical application of dendritic cell tumor vaccine should follow the principle of medical ethics.

Objectives To understand the vaccination situation of left-behind children in rural areas,provide the evidence for developing vaccination strategies for left-behind children.Methods The counties with population over 1,000,000 were selected from Anhui and Sichuan provinces respectively to conduct survery to the children who were born between the year 2003 and 2006,and had registration on vaccination book(card).Results The proportion of left-behind and outflow children are 17.94% and 6.05% respectively.The information about the children’s parents going out most came from village doctors. The proportion of the parents going out after children born was 51.60%,staying outside for more than 10 months was 78.50%. 67.97% pareets came back home during the spring festival,35.43% left-behind children went out with their parents.Conclusion Although the number of outflow children was smaller than the left-behind children,it was easy to develop immunization gap. It is the difficult part of management on EPI to migraut children. The information aboat the children’s parents going out mostly came from village doctors. It is impotant to mobilize the enthusiasm of doctors.It is especially essential that we should propagandize to the people with vaccination knowledge to the pareets came back home during spring festival.

Coronary Disease ; etiology ; psychology ; Humans