Objectives To search for the relationship between HP and lesions of gastric mucosa of children.Methods Four hundred and fifty-eight bioptic specimens-from eastrie mueosa in children were strained with hematoxylin-eosin(HE)and Warthin-Starry for light microscopic study.Results HP was detected in 152 specimens. HP deteetable rate-in specimens with normal mucosa was 1. 1%, in compari-on with 42.3% in those with chronic superficial gastritis and 14. 3% in those with chronic atrophic gastrins .Specimens with chronic active gastritls, mostry manitesting local active lesions,had HP detectable rate of as high as 80.2%、69. 7% of the specimene with HP were found to have obvious lymph follicles reaction within lamina propria in their gastric mucosa.Conclusions There is a close relationship between HP infection and Pediatric chronic gastritis(especially chronic active gastritis). Histopathologically, HP-associated gastritis is characterized by chronic and local active inflamations, and tbe formation of lymph follicles within lamina propria.

Objective To investigate the effects of astragalus polysaccharides(APS)on the metabolism of collagen in hamsters with diabetes mellitus(DM) and explore the possible mechanism of APS on diabetic cardiomyopathy. Methods The levels of insulin,C-peptide,myocardial enzymes,glycosylated serum protein(GSP),plasma and myocardial angiotensin(AngⅡ) were tested in APS group(n=15) and DM control group(n=15).Immunohistochemistry was used to measure the expression levels of typeⅠand typeⅢ collagen,activities of matrix metalloproteinases(MMPs) were examined by using zymography,and TNF-? and TGF-? mRNA were detected by RT-PCR. Results Compared with DM control group,levels of GSP,myocardial enzymes and myocardial AngⅡ were much lower in APS group(P0.05).The level of expression of collagen Ⅰ,the ratio of collagen Ⅰ/collagen Ⅲ,and the activity of pro-MMP-2 and MMP-2 in APS group were significantly lower than those in the DM group(P

Objective To explore the predictive value of serum soluble fms-like tyrosine kinase-1 (sFlt-1) and vascular endothelia growth factor (VEGF) levels in preeclampsia at second trimester. Methods Serum sFlt-1,VEGF concentrations were determined in 172 initial normal pregnant women at 26-28 gestation week.The outcomes of pregnancies were followed.In a cohort of 172 pregnant women, 16 cases of preeclampsia were developed (preeclampsia group),and 156 cases were with no complication (control group).Results The serum levels of sFlt-1 in preeclampsia group (11.4?6.2)?g/L were significantly higher than that in control group(4.5?2.1)?g/L(P＜0.01).The serum levels of sFlt-1 in precelampsia women with the onset before 32 gestation week and fetal growth retardation,(14.0?6.8)?g/L,(14.4?6.7)?g/L were significantly higher than that in women with the onset after 32 gestation week and with no fetal growth retardation (9.0?4.1)?g/L,(8.9?4.0)?g/L,respectively (P＜0.05).There was no significant difference in the serum levels of VEGF between preeclampsia group and control group.A sFlt-1 cutoff value of 8.75?g/L at 26-28 gestation week yielded a sensitivity of 87.5%,specificity of 97.4%,positive predictive value of 80.0%,negative predictive value of 88.5%,respectively,for subsequent onset of preeclampsia.Conclusion Maternal serum sFlt-1 concentration at second trimester can be used as an early predictive marker of preeclampsia.

Objective To study the relationship between tyrosine phosphorylation(TP)and protein expression of insulin receptor substrate-1(IRS-1)and insulin resistance in patients with gestational diabetes mellitus(GDM).Methods IRS-1 expression and TP in skeleton muscle tissue were determined by Western blot and immunoprecipitation in women with GDM(GDM group,n=22),normal pregnant women(normal pregnancy group,n=22)and normal nonpregnant women(normal nonpregnant group,n=13).Fasting plasma glucose(FPG)and fasting insulin(FINS)were measured by oxidase assay and immunoradioassay. Results(1)The levels of FPG,FINS,and insulin resistance index were calculated according to homeostasis model assessment [ HOMA-IR;(5.6?0.8)mmol/L,(15.4?5.1)mU/L,and 1.2?0.5 ] in GDM group were significantly higher than those in normal pregnancy group [(4.4+0.5)mmol/L,(10.6 ?3.1)mU/L,and 0.8?0.3;P

Clinical data were analysed in 19 patients with propylthiouracil (PTU)-induced antineutrophil cytoplasmic antibody associated vasculitis. Among them, 17 patients (89.5%) were diagnosed as Graves′ disease originally. The duration of PTU therapy in 18 patients was more than two years. Multi-organ involvement was common, with 15 patients (78.9%) involved in kidneys and 8 patients (42.1%) involved in lungs. The prevalences of arthralgia, skin rash and fever were also high. All patients withdrew PTU, and some with severe organ involvements received prednisone and immunosuppressant.

Objective To explore the correlation between serum brain natriuretlc peptide(BNP) and cardiac function in children with pneumonia.Methods Fifty-two children with pneumonia were seleated.Among them 20 were complicated with congestive heart failure(CHF),including 9 boys and 11 girls,their ages ranging from 4 months to 4 years old;and 32 cases non-complicated with CHF,including 17 boys and 15 girls,their ages ranging from 5 months to 6 years old.Besides,there were 30 healthy children as control group,including 16 boys and 14 girls,their ages ranging from 6 months to 5 years old.For all the children,their serum BNP was measured by using ELISA met-hod,and CK-MB was measured by automatic biochemistry analyzer.In the sametime,their cardial function was measured by echocardiography.Results In the children with pneumonina complicated with CHF,the levels of serum BNP[(182.45?57.32) ng/L] and CK-MB [(48.19?16.08) IU/L] were higher than those of healthy control group[(35.12?13.84) ng/L,(11.48?4.29) IU/L Pa0.05).The levels of left ventricular ejection fraction(LVEF),left ventricle shortening fraction(FS),peak flow velocity of arteriae arota(PFVA),peak flow velocity of pulmonary artery(PFVP) in the children with pneumonia complicated with CHF were lower than those of healthy control group(Pa0.05).There was a positive correlation between the levels of serum BNP and CK-MB(r=0.28 P0.05).Conclusion The levels of serum BNP may be an important index in diagnosis of infant pneumonia complicated with CHF.

OBJECTIVETo study the molecular mechanism of Yangjing Zhongyu Decoction (YZD) n-butanol extracts (ZDC) and ethyl acetate extracts (YSYZ) in reducing androgen in porcine granulose cells by mitogen-activated protein kinase (MAPK) pathway.

METHODSPorcine granulose cells were isolated and cultured. They were inoculated by MAPK inhibitor PD98059 at different concentrations, and then they were divided into the blank control group (0), 1, 3, 10, and 25 micromol/L groups. After 24-h culture the cytochrome P450c17a (CYP17) mRNA expression level was detected using Real-time fluorescent quantitative PCR. Contents of androgen (testosterone) in the supernate were detected using RIA and optimal PD98059 concentration screened. After intervened by 10 micromol/L PD98059 for 24 h, the culture solution was intervened by effective ingredients of with or without YZD or YSYZ at various concentrations (0, 1 , 5, 25, 50 mg/mL) at various time points (3, 6, 18, 24 h). Expression levels of p-ERK1/2, c-Fos and CYP17 were detected by Western blot. Testosterone content in the supernate was determined by radioimmunoassay (RIA).

RESULTSTen pLmol/L PD98059 could obviously decrease p-ERK1/2 protein expression and increase CYP17 mRMA expression, and elevate testosterone content in the supernate (P < 0.05). ZDC and YSYZ at 25 ng/mL could increase p-ERK1/2 protein expression and c-Fos levels, and reduce CYP17 protein expression, and lower testosterone content in the supernate after 6-h intervention (P < 0.01).

CONCLUSIONEffective ingredients of YZD could reduce androgen production in porcine granulose cells through increasing activities of MAPK.

Androgens ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Female ; Flavonoids ; Granulosa Cells ; metabolism ; Mitogen-Activated Protein Kinase 3 ; metabolism ; Mitogen-Activated Protein Kinases ; metabolism ; RNA, Messenger ; Swine

Humans ; Infant ; Neuroaxonal Dystrophies ; diagnosis

Actins ; metabolism ; Adenoma, Pleomorphic ; congenital ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Fibrosarcoma ; metabolism ; pathology ; Humans ; Infant ; Male ; Nasopharyngeal Neoplasms ; congenital ; metabolism ; pathology ; surgery ; Rhabdomyosarcoma ; metabolism ; pathology ; Salivary Gland Neoplasms ; congenital ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective: To investigate the correlation of plasma lipid with gestational diabetes mellitus (GDM) and insulin resistance in women with GDM, and to analyze the effect of plasma lipid on pregnancy outcome. Methods: A total of 922 pregnant women with GDM who underwent prenatal examination in Shanghai Sixth People's Hospital from January 2012 to April 2017 were selected for plasma lipid examination in the second trimester of pregnancy [(14.7±2.8) weeks]. Oral 75 g glucose tolerance test (OGTT) and islet function examination were performed during 24-28 weeks of pregnancy. According to the fasting triacylglycerol (TAG) level in the second trimester of pregnancy, they were divided into three groups: group T1 (TAG ≤ 1.3 mmol/L, n=310), group T2 (1.31.79 mmol/L could be used as an index to predict glucose status in GDM patients (P=0.001). ②Compared with group T1 and T2, group T3 showed significantly increased fasting glucose, fasting insulin, HOMA-IR, MBCI, and decreased WBSIS (all P<0.05). Multivariate linear regression analysis showed that BMI before pregnancy and TAG in the second trimester were independently correlated with HOMA-IR (β=0.213, β=0.224, both P=0.000). BMI before pregnancy was independently correlated with MBCI (β=0.811, P=0.000). ③The incidences of macrosomia and caesarean in group T3 were significantly higher than those in group T1 (both P<0.05). After adjusting for age and pre-pregnancy BMI, TAG in the second trimester did not increase the risk of macrosomia. TAG>4.19 mmol/L in the third trimester and TAG level in the second trimester to the third one increasing by 2.38 mmol/L or more, were high risk factors for macrosomia (both P=0.001). Conclusion: Increased TAG (TAG>1.79 mmol/L) in the second trimester is an independent and correlated factor of insulin resistance, which may aggravate GDM. Increased TAG levels (TAG increasing>2.38 mmol/L) from the second trimester to the third one and high TAG (TAG>4.19 mmol/L) in the third trimester are risk factors for macrosomia.