Objective To examine the expression of macrophage migration inhibitory factor(MIF)in lung tissues of rats with experimental pulmonary fibrosis and to discuss its effects on pulmonary fibrosis.Methods36 male SD rats were randomly divided into two groups:the pulmonary fibrosis group and the control group.Rat pulmonary fibrosis was induced by an intratracheal injection of bleomycin.The following tests were undedaken: HE and Masson staining of lung section,immunohistochemical staining of protein of MIF in the lung,RT-PCR of MIFmRNA in the lung.ResultsCompared with the control group,the mRNA and protein expression of lung MIF on day 7 to 28 in pulmonary fibrosis increased and reached the peak on day 14 after an intratracheal injection of bleomycin(P

0.05 ).But the difference of positive ratio between 1-year-old group was significant in 6 cases of B19 DNA positive (P0.05).Conclusion There are no markedly association between kawasaki disease and human parvovirus B19 infection.

ObjectiveTo study memory impairment of closed head injured(CHI) patients during later recovering period. MethodsMemory function was examined in 20 CHI patients and 20 matched healthy controls by Clinical Memory Scale(CMS). ResultsThe scores of 5 sub tests and MQ of CHI group were significant lower than that of control group, the rate of MQ ≤ 79 in CHI patients was significant higher than in controls. Conclusion There are severe memory impairment in CHI patients during later period, the impairment of recall is more serious than that of recognition.

Adult ; Female ; Glottis ; Humans ; Laryngeal Mucosa ; transplantation ; Laryngoscopy ; Laryngostenosis ; surgery ; Lasers, Gas ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Young Adult

OBJECTIVETo investigate the association of A260G and A386G polymorphisms of the DAZL gene with male infertility caused by oligozoospermia or azoospermia.

METHODSWe searched the PubMed, Science Direct, Wiley Online Library, CNKI, VIP, and CDDB databases up to November 30, 2013 for case-control studies evaluating the relationship of SNP260 and SNP386 polymorphisms of the DAZL gene with male infertility, and meanwhile conducted manual sourcing of the references in the identified studies and relevant articles. Two reviewers independently screened the title, abstract and keywords of each article retrieved. The StataSE12. 0 software was used for meta-analysis and other statistical analyses.

RESULTSTotally, 13 case-control studies were included (10 about A260G and 11 about A386G), involving 2 715 infertile patients (2 500 with oligozoospermia or azoospermia) and 1 835 normozoospermic men. DAZL A260G showed no statistical significance in the allele, dominant, recessive, co-dominant, or super-dominant gene model (P >0. 05). DAZL A386G exhibited a strong correlation with oligozoospermia or azoospermia in Asians in the allele gene model (OR = 0. 15, 95% CI 0.07 -0.34, P <0.05), dominant gene model (OR =0. 16, 95% CI 0.07 - 0. 35, P <0.05), co-dominant gene model (AA/AG) (OR = 0. 15, 95% CI 0. 06 - 0. 33, P < 0. 05), and super-dominant gene model (OR = 0. 15 (95% CI 0.06 - 0.33, P <0.05) , and so did it in Chinese in the four gene models ( OR = 0. 11, 95% CI 0.04 - 0. 28, P <0.05; OR =0. 11, 95% CI 0.04 - 0.28, P<0.05; OR = 0.09, 95% CI 0.03 - 0.26, P<0.05; OR = 0.09, 95% CI 0.03 - 0.26, P< 0.05).

CONCLUSIONOur study manifested that the DAZL polymorphism A386G, but not A260G, was correlated with reduced sper- matogenesis or sperm count specifically in Chinese males. More high-quality trials are required for a deeper insight into the exact relationship of DAZL A260G and A386G polymorphisms with oligozoospermia- or azoospermia-induced male infertility.

Alleles ; Asian Continental Ancestry Group ; Azoospermia ; genetics ; Case-Control Studies ; Humans ; Infertility, Male ; genetics ; Male ; Oligospermia ; genetics ; Polymorphism, Genetic ; RNA-Binding Proteins ; genetics ; Spermatozoa

Animals ; Coxsackie and Adenovirus Receptor-Like Membrane Protein ; Coxsackievirus Infections ; genetics ; metabolism ; Disease Models, Animal ; Enterovirus B, Human ; pathogenicity ; Heart ; drug effects ; Immunohistochemistry ; Interleukin-1beta ; administration & dosage ; pharmacology ; Male ; Mice ; Mice, Inbred BALB C ; Myocarditis ; drug therapy ; genetics ; metabolism ; pathology ; virology ; Myocardium ; metabolism ; pathology ; RNA, Messenger ; Receptors, Virus ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction

Hospitals ; statistics & numerical data ; Humans ; Population Surveillance ; Quality Control ; Wounds and Injuries ; epidemiology

Triptolide (TPT), an active compound extracted from Chinese herb Tripterygium wilfordii , has been used in therapy of rheumatoid arthritis (RA). In this study, after synovial fibroblasts from rheumatoid arthritis (RASFs) were treated with TPT, we investigated its effect on the differentiation of Th17 cells. Firstly, the mRNA level of cyclooxygenase (COX) wad detected by qRT-PCR and the protein level of prostaglandin E2 (PGE2) was tested by ELISA in RASFs treated with different concentrations (0, 10, 50, 100 nmol L-1 ) of TPT. Then after TPT pre-treated RASFs and RA CD4 + T cells wer e co-cultured for 3 days in the presence or absence of PGE2, IL-17 and IFN-gamma production in CD4 T cell subsets were detected by flow cytometry. The results showed TPT decreased the mRNA experssion of COX2 and the secretion of PGE2 in RASFs in a dose-dependent manner(P <0. 05). We further found that differentiation of Thl7 cells was downregulated in a dose-dependent manner, and exogenous PGE2 could reverse the inhibition of Th17 cell differentiation(P <0. 05). Taken together, our results demonstrated that TPT inhibited the mRNA level of COX2 and the secretion of PGE2 in RASFs, which partly led to impaired Th17 cell differentiation in vitro.
Arthritis, Rheumatoid ; drug therapy ; enzymology ; immunology ; Cell Differentiation ; drug effects ; Cell Line ; Cyclooxygenase 2 ; genetics ; metabolism ; Dinoprostone ; metabolism ; Diterpenes ; pharmacology ; Epoxy Compounds ; pharmacology ; Fibroblasts ; drug effects ; immunology ; Gene Expression Regulation, Enzymologic ; drug effects ; Humans ; Middle Aged ; Phenanthrenes ; pharmacology ; Synovial Fluid ; drug effects ; Th17 Cells ; drug effects ; pathology

Objective To investigate influence of nano-?-linolenic acid on expression of macrophage migration inhibitory factor (MIF) in murine model with viral myocarditis (VM).Methods Eighty male Balb/c mice were randomly divided into 4 groups equally:control group,model group,low and high dose intervention group.Mice in control group were inoculated introperitoneally with Eagle's solution.Every mouse in other groups was treated with 0.1 mL Coxsackie B3 virus(CVB3) intraperitoneally.Then,mice in low and high dose intervention group were treated with 60 and 180 mg/kg nano-?-linolenic acid solution for 1 week,respectively.Mice in control group and model group were treated with 9 g/L saline for 1 week.All mice were killed on day 15.The expression levels of myocardial MIF mRNA and protein were detected by reverse transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry.Serum MIF concentration were exa-mined by enzymelinked immunosorbent assay(ELISA).Myocardial histopathology was determined with hematoxylin and eosin stain.Results The mortality rate was 0,45%,30% and 20% in control group,model group,low and high dose intervention group,respectively.The mortality rate was significantly lower in high dose intervention group compared with model group (P

0.05),but that MFI and/or PPC of CAR in the 2 types cells markedly increased compared with lymphocytes in the same group(Pa