1.Effects of rehabilitation training on the expression of S-100,GFAP and Nestin in the cortex of rats with cerebral infarction
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(09):-
Objective To study the effect of rehabil it ation training on the expression of S-100, GFAP and Nestin in the cortex of cer ebrally infarcted rats. Methods Sixty Sprague-Dawle y rats were used. Experimental cerebral infarction was caused in all the rat s. 24 hours after the infarction, all the rats were randomly divided into 3 grou ps: a rehabilitation group, an immobiliaztion group and a control group. The rat s in the rehabilitation group were given balancing, grasping, rotating and walki ng exercises everyday, while those in the immobilization group were immobilized in cages. The rats in the control group was without any treatment. Immunohistoc hemical technique was used to detect the S-100, GFAP and Nestin expression at t he time points of 24 hours, 1, 2, 3 and 4 weeks after infarction, respectively. Results S-100, GFAP and Nestin expression were foun d around the infarcted cortex. The response increased as the extension of the su rviving time. The immunohistochemical reaction in rats in the rehabilitation gro up was stronger than that in the immobilization group. Conclusi on Rehabilitation training can activate astrocytes, which could impr ove the internal environment, protect the neurons and promote repairing of neuro n.
3.Combined fixation for comminuted intra-articular fracture in distal radius
Orthopedic Journal of China 2006;0(08):-
[Objective]To explore the effective treatment method of the articular fracture in distal radius.[Method]Severely comminuted AO type-C3 intra-articular fractures of the distal end of the radius were treated with combined internal and external fixation.The Gartland and Werley system and the Green and O'Brien system were used for comparison of the pre-operative and after operative evaluation.Total articular congruity had been assessed with both clinical rating systems.[Result]At a mean of ninteen months following-up,the mean arc of flexion-extension was 78% of that on the uninjured side and the mean grip strength was 82% of that on the uninjured side.The mean total articular incongruity(the gap plus the step-off)averaged 2 mm,and the radial length was restored to a mean of 11 mm.According to the Gartland and Werley demerit-point system,seventeen of the patients had a good or excellent result.According to the modified Green and O'Brien clinical rating system,eleven had a good or excellent result.Postoperative total articular incongruity had a moderately strong correlation with the outcome as assessed with both clinical rating systems.[Conclusion]Open reduction and combined internal and external fixation of AO type-C3 fractures can restore radiographic parameters to nearly normal values,maintain reduction throughout the period of fracture-healing,and provide satisfactory functional results.
4.The value of diagnosis and differential diagnosis of gallbladder wall thickening diseases with DWI
Sheng ZHAO ; Hua SUN ; Li GUO ; Kui LONG ; Weihua LI
Journal of Practical Radiology 2017;33(8):1209-1212
Objective To evaluate the usefulness of diffusion-weighted imaging(DWI) in the diagnosis and differential diagnosis of gallbladder wall thickening diseases.Methods 42 patients with gallbladder wall thickening (16 patients with carcinoma and 26 patients with benign lesion) were included in this study.All patients performed conventional MRI and DWI.The diagnostic performances of three methods (conventional MRI,visual assessment of color fusion image from DWI and T2WI,and ADC measurement) were evaluated by two radiologists.Results The area under the receiver operating characteristic curve were 0.570,0.849,0.901 for conventional MRI,visual assessment and ADC measurement respectively.The accuracy,sensitivity and specificity were 59.5%,62.5%,57.7% for conventional MRI,85.7%,81.2%,88.5% for visual assessment of color fusion image,and 83.3%,80.0%,85.2% for ADC measurement,respectivily.The mean ADC value of gallbladder cacinoma[(1.15±0.35)×10-3mm2/s]was significantly less than that of gallbladder benign lesion [(1.99±0.61)×10-3mm2/s](P<0.01).Conclusion The DWI(visual assessment of color fusion image and ADC measurement)might be a useful tool for diagnosis and differential diagnosis of the gallbladder wall thickening diseases.
5.Research progress of treatment for amblyopia
Hua, LI ; Qing-Ling, XIANG ; Meng, LIAO ; Long-Qian, LIU
International Eye Science 2011;11(3):385-387
The most common forms of treatment for amblyopia are occlusion therapy and pharmacologic penalization. But these methods can not recover all the visual deficits of amblyopes and there are some disadvantages of the treatment which need to be considered, including adverse effects, compliance and social stigma. Recently, some novel approaches are investigated for improving the effectiveness and compliance of treatment for amblyopia.
6.Curriculum of Assistive Technology in Higher Vocational Education School
Qilei TU ; Xin FANG ; Hua LONG ; Gaofeng LI ; Baolin XIONG
Chinese Journal of Rehabilitation Theory and Practice 2011;17(5):488-490
This article discussed the problem of assistive technology curriculum in higher vocational education school, and the reform in the future.
7.The therapeutic effect of PNF on shoulder subluxation after stroke
Hua LI ; Yulong WANG ; Jianjun LONG ; Fei YANG
Chinese Journal of Physical Medicine and Rehabilitation 2009;31(3):173-176
Objective To investigate the curative effects of selected proprioceptive neuromuscular facilitation (PNF) patterns and techniques on shoulder subluxation soon after strokd. Methods Forty-one stroke patients with shoulder subluxation were randomly divided into a treatment group(n=22) and a control group (n=19). The patients in the treatment group receiveed the selected PNF techniques daily for 4 weeks, while the patients in the control group were treated with their previous therapeutic protocol. The shortest distance from the lower border of the acromion to the nearest point of the humeral head(AHH) was measured. Brunnstron grading was used to evaluate motor function in the affected upper linb, and a visual analogue scale (VAS) to assess subLuxation. Shoulder pain before and after treatment was also assessd. Results There was no significant difference in general condition between the patients in the two groups before treatment. In the treatment group, a significant improvement in AHH distance of the affected shoulder was observed after 4 weeks. Shoulder subluxation waw cured in 13 patients, with AHH distance <0.75 cm after 4 weeks of treatment. Conclusion The PNF technique applied was effective for the early treatment of subluxation after stroke. Motor function in the affected upper limb could be improved significantly and pain alleviated.
8.Studies on the Bacterial Diversities of the Hot Springs at Tengchong Rehai in Yunnan Province China
Qin-Yuan LI ; Xiao-Long CUI ; Dong-Hua ZHANG ; Qian PENG ; Li-Hua XU ;
Microbiology 1992;0(05):-
Bacterial biodiversities of microbial mat and sediments, which were sampled from thermal springs of Tengchong Rehai in Yunnan, were preliminarily studied with PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Directly extracted total DNA from environmental samples amplified by PCR with two sets of bacteria-specific primers. The PCR products, which include the V 8 and V 9 high-variable regions respectively, were analyzed by using DGGE. The DGGE profiles not only indicated the existence of higher levels of bacterial diversity, but also showed that the microbial mat and sediments have different dominant bacteria. Furthermore, the bacterial PCR-DGGE displayed clear profiles of bacterial structure selected by the key abiotic factors of the extreme environments, such as temperature and concentration of oxygen.
9.Relationship between rats' medullary astrocytes and neurons after exposed to infrasound
Hua YUAN ; Hua LONG ; Ling LI ; Xiang MOU ; Jianyong QIU ; Jing LIU ; Jingzao CHEN
Chinese Journal of Rehabilitation Theory and Practice 2002;8(11):661-663
ObjectiveTo observe the relationship between astrocytes and neurons in rat's medulla oblongata after exposed to 8Hz, 90dB/130dB infrasound for different times. MethodsRats were exposed to 8Hz, 90dB/130dB infrasound for 2 hours per day.At the 1st,7th,12th,21th and 28th day, the expression of glial fibrillary acidic protein (GFAP) and Fos in medulla oblongata were detected using double-labling immunohistochemical method.Results GFAP-positive astrocytes and Fos-positive neurons were increased after exposure for one time. They showed similar topographic localization and formed close relationship. Their number increased as the exposure times increasing, and decreased gradually after 14 days. The reaction of 130dB group was stronger than that of 90dB group.Conclusions 8Hz, 90dB/130dB infrasound can activate both astrocytes and neurons in rats' medulla oblongata which might participate and regulate the response to infrasound.
10.Predictive value of fluid overload for mortality in children with severe sepsis
Jiao CHEN ; Long XIANG ; Zhenjiang BAI ; Jun HUA ; Ying LI ; Xiaozhong LI ; Yanhong LI
Chinese Journal of Applied Clinical Pediatrics 2016;31(10):755-759
Objective To investigate the predictive value of fluid overload for mortality in children with severe sepsis.Methods In this retrospective study,the children with severe sepsis who were admitted to the Pediatric Intensive Care Unit (PICU),Children's Hospital of Soochow University between January 2011 and March 2015.Fluid accumulation was calculated in the first 72 hours after admission.Pediatric index of mortality Ⅱ (PIM2) score was calculated during the first 1 hour after admission.Multivariate Logistic regression analysis assessed the relationship between fluid overload and mortality after adjustment for confounding factors.The predictive value of fluid overload for mortality was assessed by the receiver operating characteristic curve and au area under the receiver-operating-characteristic curve (AUC).Results Of the 199 children admitted,62 cases (31.2%) died during PICU stay.Among the children,133 cases (66.8%) had fluid overload of<5%,55 cases (27.6%)had fluid overload of≥5%-10%,and 11 cases (5.6%) had fluid overload of≥ 10%.Multivariate regression analysis showed that a high fluid overload percent (OR =1.263,95 % CI:1.113-1.434,P < 0.001),a high PIM2 score (OR =1.028,95 % CI:1.012-1.043,P < 0.001) and multiple organ dysfunction syndrome(OR =4.160,95% CI:1.728-10.012,P =0.001) were independent risk factors for mortality in children with severe sepsis.The fluid overload was significantly associated with mortality (OR =1.309,95% CI:1.158-1.480,P <0.001),even after adjustment for age and illness severity assessed by PIM2 scores.Fluid overload achieved AUC of 0.741 (95% CI:0.661-0.820,P < 0.001) for predicting mortality in children with severe sepsis.Conclusion Fluid overload developed during the first 72 hours after admission is independently associated with and predictive of PICU mortality in children with severe sepsis.