Objective To assess the effect of cryopreservation of cornea in glycerol,to analyze the protective effect of sodium hyaluronate on corneal endothelial cells(CEC) during preservation,and to find out a convenient and cheap method for the storage of cornea.Methods 20 rabbit corneas were divided into two groups randomly.The corneas of control group were stored directly in pure glycerol at-25℃,while in the experimental group sodium hyaluronate was evenly anointed on the corneal endothelial surface of the cornea before being cryopreserved in glycerol.2 months later,the effect of preservation for both groups was examined with trypan blue combined with alizarin red staining(viability staining) for corneal endothelial cells,electron-microscope examination for preserved corneas,and rabbit experimental penetrating keratoplasty(PKP) with the long-term cryopreserved corneas from both the experimental and control groups.Results CEC viability could be preserved by both methods.CEC trypan blue combined with alizarin red staining(viability staining) showed that the CEC density of cornea in the experimental group was 3 067.9?127.6/mm2,which was higher than that of control group(2 899.9?134.0/mm2).The mortality of CEC in experimental group was 4.2%?3.4%,lower than that in control group(13.8%?2.2%).Suceessful rate of PKP was higher in the experimental group with higher CEC density.Conclusion Cryopreservation with glycerol can preserve the viability of CEC.Sodium hyaluronate can reduce the damage to endothelium in the preserving procedure and can obviously improve the effect of preservation.The improved cryopreservation of cornea with glycerol may be a convenient,economic and effective method for eyebank.

Objective:To compare the intravenous (Ⅳ) administration with an oral iron agent administration in their efficacy and side effects in hemodialysis patients with renal anemia. Method:50 renal anemic patients were randomly grouped as the treatment group (TG) and controlled group (25 cases for each). 100 mg iron dextran injection was intravenously dropped twice weekly for the TG patients in the dialysis course, totally 10 times. 150 mg iron polysaccharide complex capsules was orally used qd in the controlled group. All the patients were treated in combination with 8000u of erythropoietin (EPO) weekly and observed for 8 weeks. The changes in Hb, serum ferritin level and agent side effect were monitored. Result:Hb, Hct and SP were all improved in both the TG and controlled group after the treatment (P

Objective To evaluate the effect of FTY720 on retinal leukocytes adhesion and vascular permeability in diabetic retinopathy (DR) model,and explore its mechanism.Methods Ninety male Wister rats were randomly divided into normal control group,diabetic group and FTY720 group,thirty rats in each group.Diabetes was induced by giving a single intraperitoneal injection of streptozocin.FTY720 group was administered with FTY720 at a dose of 0.3 mg/kg by oral gavage daily for 3 months after establishment of diabetes.All rats were used for experiments following intervention for 3 months in FTY720 group.Immunohistochemical staining was used to observe the expression and distribution of intercellular adhesion molecule (ICAM-1) and vascular cell adhesion molecule (VCAM-1),and the positive cells were counted.Real-time reverse transcription PCR was used to measure mRNA expression of ICAM-1 and VCAM-1.Fluorescein isothiocyanate-Concanavalin A perfusion was used to detect retinal leukocytes adhesion.Evans blue (EB) perfusion was used to analyze retinal vascular permeability.Immunofluorescence staining was used to detect retinal inflammatory cells infiltration.Results In diabetic group,both ICAM-1(t =12.81) and VCAM-1 (t=11.75) positive cells as well as their mRNA expression (t=16.14,9.59) were increased compared with normal control group,with statistical significance (P＜ 0.05).In FTY720 group,both ICAM-1(t=-9.93) and VCAM-1 (t=-6.61) positive cells as well as their mRNA expression (t=-15.28,-6.10) were decreased compared with diabetic group,with statistical significance (P＜ 0.05).Retinal leukocytes adhesion (t=16.32) and EB permeability (t=17.83) were increased in diabetic group compared with normal control group,while they were decreased in FTY720 group compared with diabetic group(t=-9.93,-11.82),with statistical significance (P＜0.05).There were many CD45 positive leukocytes infiltration in retina of diabetic group,including CD11b positive macrophage/activated microglia,while both of them were little in FTY720 group.Conclusions FTY720 can decrease retinal leukocytes adhesion,reduce retinal vascular permeability and inflammatory cells infiltration,which is associated with down-regulation of ICAM-1 and VCAM-1.

OBJECTlVE To investigate the improvement effect of rosigIitazone(ROm)against cis-pIatin(DDP)-induced nephropathy in rats and its mechanim of resistance to oxidative stress. METHODS Invitro:the survivaI rate of HEK293 ceIIs was measured with mTT assay incubated with DDP 0.4 -100 μmoI·L-1 aIone or with DDP 25 μmoI·L-1 and ROm 0.01-1000 μmoI·L-1 for 48 h. Then the content of maIondiaIdehyde(mDA)and the activity of gIutathione(GSH)in HEK293 ceIIs co-incubated with DDP 25 μmoI·L-1 and ROm 100 μmoI·L-1 were assayed using chromatometry. In vivo:sixty maIe SD rats were randomIy divided into normaI controI group,modeI group(DDP 5 mg·kg-1 )and modeI+ROm 5,10 and 20 mg·kg-1 groups. The modeI of DDP-induced nephropathy was estabIished by taiI vein injection of DDP 5 mg·kg-1 ,once a week,for 3 times. After the first injection,the rats in modeI+ROm 5,10 and 20 mg·kg-1 groups were ig given ROm 5,10 and 20 mg·kg-1 ,once a week,for 8 weeks. The bIood urea nitrogen(BUN)and creatinine(Cr)were assessed with an automatic biochemicaI anaIyzer. Nitric oxide(NO)and mDA content,GSH and superoxide dismutase( SOD)activities in the kidney tissue were determined using chromatometry and histopathoIogicaI changes in renaI tissues were detected by hematoxyIin-eosin staining. RESULTS In vitro:IC50 of DDP to HEK293 ceII survivaI for 48 h was 21.0 μmoI·L-1 . Co-incubation with ROm 1-1000 μmoI·L-1 and DDP 25 μmoI·L-1 for 48 h couId signifi-cantIy increase the totaI ceII survivaI rate compared with DDP 25 μmoI·L-1 group(P﹤0.05,P﹤0.01), whiIe co-incubation with ROm 100 μmoI·L-1 and DDP 25 μmoI·L-1 for 48 h couId significantIy decrease the IeveI of mDA(P﹤0.01)and increase GSH activity(P﹤0.01),respectiveIy. In vivo:compared with normaI group,serum BUN and Cr content in modeI group were significantIy increased(P﹤0.01),renaI tissue GSH and SOD activities decreased(P﹤0.01),renaI tissue mDA content increased(P﹤0.01), and renaI tissue NO IeveI and NOS activity aIso decreased significantIy(P﹤0.05). Compared with modeI group,serum BUN content in modeI+ROm 5,10 and 20 mg·kg-1 decreased from(17.0±1.3)mmoI·L-1 to 14.0±4.1,11.2±1.8 and(6.1±1.0)mmoI·L-1(P﹤0.01),serum Cr content in modeI+ROm 10 and 20 mg·kg-1 decreased from(124.6±39.8)mmoI·L-1 to 49.0±5.2 and(47.1±2.9)mmoI·L-1(P﹤0.01), renaI tissue GSH and SOD activities in modeI+ROm 10 and 20 mg·kg-1 group increased significantIy (P﹤0.05,P ﹤ 0. 01),mDA IeveI decreased(P﹤0.05,P ﹤ 0. 01),NO IeveI and NOS activity aIso increased(P﹤0.05,P﹤0.01),and renaI pathoIogicaI tissue damage couId be improved. CONCLUSlON ROm pIays a protective roIe in rat kidney damage caused by DDP,which may be reIated to improving oxidative stress resistance of the kidney.

Objective To compare the effects of intravitreal tamponade of C3 F8 with silicon oil on postoperative vitreous hemorrhage and visual prognosis after vitrectomy for proliferative diabetic retinopathy (PDR).Methods The clinical data of 121 patients (127 eyes)who underwent primary vitrectomy due to PDR were analyzed retrospectively.All the patients were divided into two groups according to different intravitreal tamponade, including C3 F8 tamponade group (53 patients with 56 eyes ) and silicone oil tamponade group (68 patients with 71 eyes).There was no difference of gender (χ2 = 0.956 ),age (t =1.122),duratiion of diabetes (t=0.627),fasting blood glucose (t=1.049),systolic pressure (t=1.056), diastolic pressure (t = 0.5 1 7 ), history of hypertension (χ2 = 0.356 ), nephropathy (χ2 = 1.242 ), preoperative laser photocoagulation (χ2 = 1.225 )and All the patients underwent three port pars plana vitrectomy.The mean follow-up was 2 years ranging from 6 months to 4 years.And then the incidence and onset time of postoperative vitreous hemorrhage and postoperative BCVA of the two groups were compared. Results Postoperative vitreous hemorrhage occurred in 14 of 56 eyes (25.00%)in C3 F8 tamponade group. The average onset time of postoperative vitreous hemorrhage were (64.64 ± 59.09)days ranging from 7 -225 days and mostly were within 30-60 days (35.71%,5/14).Postoperative vitreous hemorrhage also occurred in 7 of 71 eyes (9.89%)of silicone oil tamponade group after silicone oil removal with an average onset time of (25.29±20.46)days ranging from 3-65 days and were mostly within 1 5-30 days (42.86%, 3/7).There was a significant difference in the incidence of postoperative vitreous hemorrhage between the two groups (χ2 = 5.200,P <0.05 ).BCVA of the two groups was improved significantly after operation (Z =2.472,3.1 14;P <0.05).Postoperative BCVA of silicone oil tamponade group was poorer than C3 F8 tamponade group (Z =1.968,P <0.05).Conclusion Both C3 F8 and silicone oil tamponade can improve the visual acuity after vitrectomy for PDR.Compared with C3 F8 ,silicone oil tamponade had lower incidence and late onset of postoperative vitreous hemorrhage after vitrectomy for PDR.

AIM:To explore the effect of comprehensive therapy in the treatment of fungal corneal ulcer and clinical outcome.
METHODS:A total of 159 cases 159 eyes fungal corneal ulcer patients took comprehensive therapy of 10 measures, followed up more than 3mo to observe the treatment effect and the final visual acuity in our hospital for treatment.
RESULTS: The average hospitalization time of fungal corneal ulcer was(12. 11±5. 13) d. A total of 148 cases of 148 eyes were cured, and the cure rate was 93. 1%;improvement in 8 cases, accounted for 5. 0%; 3 cases were ineffective, accounted for 1. 9%. The final visual acuity of 89 eyes ≥0. 3, accounted for 56. 0%(89/159). The final visual acuity of 136 eyes ≥0. 1, accounting for 85. 5%(136/159), and accounting for 91. 9% in 148 cured patients.
CONCLUSION: The comprehensive therapy in the treatment of fungal corneal ulcer has higher cure rate and visual acuity.

Objective To investigate the potential role of FoxP3-expressing CD4+CD25+ regulatory T cells in children with asthma.Met-hods Intracellular 3-colour flow cytometry and real time PCR were used to assess the expression of FoxP3 in peripheral blood mononuclear cell(PBMC)of asthma,repectively.Results There were similar frequencies of CD4+ CD25+ T cells in peripheral blood of asthma patients and healthy subjects.However,the percentage of CD4+CD25high and CD4+CD25+FoxP3+ in asthma group were lower than those in healthy subjects(Pa

With social progress and improvement of human health consciousness,the medical device industry of China has been fast developing,and a new request for the specialized talents cultivation has been put forward.Based on the consideration of the development of medical device industry and the students,a cultivating mode for the medical engineering talents is explored in this paper through the major course reform,training double-quality teachers and cooperation between industry,university and research institutes.

AIM:To optimize the technical parameters indica-daisy dropping pill(Flos chrysanthemi indici) through controlling the influencing factors. METHODS:To take weight variation of pills,comprehensive quality and disintegration time limited as index,the above factors were observed by orthogonal test. RESULTS:Good technological parameters of indica-daisy dropping pill were as follows PEG6000∶PEG400=54∶6 as matrix,dimethicone as refrigerant.Temperature of drug fluids of 70 ℃,internal and external diameter of dropper within 6.2 mm and 9.0 mm,proportion of drug and matrix(1∶3),30 dropping per minute.The weight variation of pills was small,type quality was good and disintegration time limited was short.The water absorbability was smaller than that of granules. CONCLUSION:The finished products are of good quality and appropriate for mass production.

Purpose:To investigate the correlation between the expression of gene protein of c-fos,bcl-2 and gastric cancer and to provide the evidence of molecular pathology for grouping and early diagnosis.Methods:The expressions of gene-protein of c-fos,bcl-2 of the resected specimen of 54 patients with gastric carcinoma were detected by immunohistochemical method of S-P.All of the data were input into computer by system of Excel and SAS for statistical analysis.Results:The expressions of gene protein of c-fos,bcl-2 in intestinal type of gastric carcinoma were significantly higher than those in diffuse type (P=0.012, 0.0001, respectively).The expressions of gene protein of c-fos ,bcl-2 in the group of lymphaden metastasis were higher than that in the group of no lymphaden metastasis (c-fos 63.64%,54.55%;bcl-2 50.00%,27.27%), but no statistical significance.The expressions of c-fos , bcl-2 in atypical hyperplasia tissue nearby carcinoma and high differentiated carcinoma were stronger.Conclusions:The expressions of gene protein of c-fos,bcl-2 in intestinal type of gastric carcinoma were significantly higher than those in diffuse type.It showed that the pathogenesis of gastric carcinoma have difference between the intestinal type and diffuse type and it maybe provide proofs for gastric carcinoma grouping.The expression of c-fos,bcl-2 can be used as the molecular pathological symbols for early diagnosis.