1.Effect of Tanshinone II A on Cytokines of Rats with Severe Acute Pancreatitis Lung Injury.
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(11):1361-1366
OBJECTIVETo explore the effect of Tanshinone II A on severe acute pancreatitis (SAP) lung injury (ALI) rats and its possible mechanism.
METHODSSD rats were injected with sodium taurocholate to induce SAP group, and then intervened with sodium tanshinone II A sulfonate ( STS group). Simultaneously a sham-operation group (SO group) was set up. There were 24 rats in each group. The survival state and wet-to-dry weight ratio of lung tissues were observed. Activities of myeloperoxidase (MPO) in lung were determined by MPO reagent kit. Pathologic changes of lung tissues were determined by Hofbuaer method. Expression levels of three cytokines, TNF-alpha, IL-1beta, and intercellular cell adhesion molecule-1 (ICAM-1) were detected by ELISA.
RESULTSThe survival state of rats in the SAP group was deteriorated. The wet-to-dry weight ratio, MPO activities, pathologic changes in lung tissues, and expression levels of TNF-alpha, IL-1beta, and ICAM-1 increased significantly more in the SAP group than in the SO group (P < 0.05). Compared with those in the SAP group, the survival state of rats in the STS group was improved; the wet-to-dry weight ratio, MPO activities, pathologic changes in lung tissues, and expression levels of TNF-alpha, IL-1beta, and ICAM-1 obviously decreased in the STS group (P < 0.05).
CONCLUSIONTanshinone II A had remarkable effect on SPA LI rats, which might be associated with changing cytokines levels and attenuating infiltration of lung inflammatory cells.
Acute Lung Injury ; drug therapy ; Animals ; Cytokines ; metabolism ; Diterpenes, Abietane ; pharmacology ; therapeutic use ; Intercellular Adhesion Molecule-1 ; Interleukin-1beta ; Lung ; Pancreatitis ; drug therapy ; Peroxidase ; Rats ; Rats, Sprague-Dawley ; Taurocholic Acid ; Tumor Necrosis Factor-alpha
2.Biochemical characterization and comparison of recombinant RNase HIIa and RNase HIIb from Chlamydia pneumoniae
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To clone and compare RNase HIIa and RNase HIIb of Chlamydia pneumoniae AR39(CpRNaseHIIa and CpRNaseHIIb). Methods Genes of CpRNase HIIs were amplified with the designed primers.Then,the recombinant plasmids were constructed,and CpRNase HIIs were expressed and purified with pET expression system.The 5′-32P-labeled RNA/DNA substrate and DNA with oligoribonucleotides substrates were prepared to identify characterization of CpRNase HIIs. Results Ribonuclease H activity of both CpRNase HIIa and CpRNase HIIb could cleave oligoribonucleotides strand,generating break with 3′-OH and 5′-phosphate ends.The results showed that there was different biochemical characterization between them. Conclusion CpRNase HIIa and CpRNase HIIb have different functions in C.pneumoniae.
3.Determination of 2,3,5,4′-Tetrahydroxystilbene-?-O-?-D-Glucoside in Radix polygoni multiflori preparata by HPLC
Yun LIU ; Dong ZHANG ; Hua YUAN
Chinese Traditional Patent Medicine 1992;0(11):-
AIM: To establish HPLC for determination of 2,3,5,4′tetrahydroxystilbene-?-O-?-D-glucoside in Radix polygoni multiflori preparata. METHODS: The column was diamonsil C_(18)(5 ?m,4.6 mm?250 mm).The mobile phase was CH_3CN-CH_3OH-H_2O(10∶20∶70) and ?=320 nm.The flow rate was 1.5 ml/min. RESULTS: The linearity was in the range of 0.0253-0.3542 ?g.The average recovery and RSD were 99.34% and 0.49%,respectively. CONCLUSION: The method is simple,precise and reproduciable and can be used for determination of 2,3,5,4′-tetrahydroxystilbene-?-O-?-D-glucoside in Radix polygoni multiflori preparata effectively.
4.The Influence of Thy-1.1 Stem Cell Transplantation on Neointimal Formation of Injured Artery
Shao-Hong DONG ; Hua-Dong LIU ; Xin JIANG ;
Chinese Journal of Hypertension 2006;0(11):-
Objective To study the effect of Thy-1.1 stem cell transplantation on endothelial hyperplasia and restenosis.Methods Thirty 4-6 weeks male SD rats were sacrificed to obtain the Thy-1.1 stem cells.Carotid artery were injured by ballon in sixty female SD rat's were randomized to receive stem cell transplantation(5?10~6 Thy-1.1,n=30)or saline approach(n=30).About 5?10~6 Thy-1.1 stem cells were injected into the injured arter- y after carotid artery injury;while the control rats underwent carotid artery injury and was injected the same amount of saline.The animals were sacrificed,3,7,14,21 and 28 days after balloon denudation.The samples of carotid artery were harvested for pathological examination,RT-PCR and in situ hyhridzation(ISH)were used to detect the transplanted cells in the injured artery.Results The intimal thickness was thinner in stem cell transplantation group(I/M,Stem cell transplantation group:2.06?0.28 vs control group 2.42?0.19,P
6.Early radiological changes and effect on prognosis in preterm infants with cerebral white matter damage.
Chang LIU ; Jian-hua FU ; Xin-dong XUE
Chinese Journal of Pediatrics 2012;50(10):762-766
Brain
;
diagnostic imaging
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pathology
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Cerebral Palsy
;
diagnosis
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pathology
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Cognition Disorders
;
diagnosis
;
pathology
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Early Diagnosis
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Humans
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Infant, Newborn
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Infant, Premature
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Infant, Premature, Diseases
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diagnosis
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pathology
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Language Disorders
;
diagnosis
;
pathology
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Leukomalacia, Periventricular
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complications
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diagnosis
;
pathology
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Magnetic Resonance Imaging
;
methods
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Prognosis
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Radiography
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Severity of Illness Index
7.Effect of simple PKP treatment of osteoporotic thoracolumbar vertebral compression on serum leptin and bone mineral density in patients with fracture
Fanguo LIN ; Dong LIU ; Yongming SUN ; Jun HUA ; Zhaoyao YANG
The Journal of Practical Medicine 2016;32(11):1794-1796
Objective To investigate the effect of percutaneous vertebroplasty and kyphoplasty (PKP) on bone density and serum leptin in patients with osteoporotic thoracolumbar compression fractures. Methods One hundred and two patients with osteoporotic thoracolumbar vertebral compression fractures and 100 cases of healthy control were treated with PKP surgery. The serum leptin and adiponectin, and the bone mineral density of the lumbar spine were determined. Results patients Levels of the serum leptin and adiponectin in patients with osteoporotic thoracolumbar vertebral compression fracture were significantly higher than those with non osteoporotic fracture patients (P < 0.05). One month post-PVP, the levels of serum leptin and adiponectin in the two groups were significantly decreased (P < 0.05). postoperative group of The lumbar bone density at 1 month post-operation was is significantly higher than that before operation in each group (P < 0.05, respectively), with no significant difference between the two groups. The levels of serum leptin and adiponectin were negatively correlated with the bone mineral density (BMD) (P < 0.05). Conclusions tLevels of serum adiponectin and leptin were both increased in osteoporotic or non bone osteoporosis thoracolumbar vertebral compressor fractures. The percutaneous vertebroplasty forming operation could effectively reduce the serum level of leptin and adiponectin, and improve the bone mineral density of the lumbar vertebrae.
8.Effects of Simvastatin on Early Oxidative Stress and Caveolin-1 in Apolipoprotein E-Deficient Mice
Dong-Hua YIN ; Ming GUI ; Meng LIU ; Jun HUANG ;
Chinese Journal of Hypertension 2006;0(08):-
Background Rare studies on the effect of statin on early stage of atherosclerosis have been repor- ted.Oxidative stress induced endothelial dysfunction may be the initiative factor for the development of atheroscle- rotic plague.Objective To investigate the mechanisms by which simvastatin,prevents atheroselerosis independ- ently of its lipid-lowering effect in Apolipoprotein E deficient mice.Methods Twenty-four 6 week old male apoE- deficient mice were randomly to receive placebo or simvastatin 5 mg/(kg?d)by gavage for 4 weeks.Total choles- terol(TC),super oxide dismutase(SOD),malondialdehyde(MDA)and serum nitric oxide(NO)were measured by biochemical analysis.Endothelium was observed by HE dyeing.The expression of caveolin-1 in aortic wall was detected by immunohistochemistry.Results There was no significant difference in serum TC between control and simvastatin treatment groups.Simvastatin caused less damaged endothelium(33.33% vs control's:75%,P
9.Application Research on the Production of Ergosterol using Corn Straw Hydrolyzates Fermentation by Yeast
Gong-Ming SONG ; Jiao LIU ; Dong-Hua XUE ;
Microbiology 2008;0(12):-
Biomass is a renewable resource, which can be transformed into useful chemical products. The effects of dilute hydrochloric acid on the hydrolysis of steam explosion pretreatment of corn straw were studied. This article developed the application research of ergosterol using corn straw hydrolysates as fer- mentation substrates. The results showed that when corn straw was hydrolyzed with 1.5% hydrochloric acid, temperature at 90?C, hydrolysis for 3 h and the corresponding solid to liquid ratio at 10%, the reducing sugar content can reached up to 53.3% and cellulose conversion efficiency was 79%. The optimal fermental pa- rameters were as follows: 6.0 oBx of corn straw hydrolysates, corn concentration steep water at 4%, pH 7.5, 10% of inoculation, 28?C cultivated for 32 h. Under these conditions, the yeast biomass up to 8.5 g/L and the ergosterol content up to 2.35%. The infrared spectrometer and the X-ray diffract meter used to characterize of crystallite structure.
10.Study on Glycolic Acid Oxidase (GO) of Spirulina(Arthrospira)
Zhi-Zhong WANG ; Dong-Hui GONG ; Hua LIU ; Chen QIAO ;
Microbiology 1992;0(04):-
A comparative study on the glycolic acid oxidase (GO) of the domestic Spirulina(Arthrospira) platensis (S_(1)) from alkaline lake in Erdos Plateau and the imported S. (A.) platensis (S_(2)) and S. (A.) maxima (S_(3)) as well is made with colorimetric method. The results show that activity of GO (25℃, pH 8.0) of S_(1), S_(2 )and S_(3) is 70.9 U/gFW, 59.6 U/gFW and 80.9 U/gFW respectively; the GO's optimum temperature of S_(1)、S_(2) and S_(3 )is 30℃; the GO's optimum pH value of S_(1 )is 8.6,while that of S_(2 ) 8.2 and that of S_(3) 8.4; the GO of S_(1 )is stable from 0℃ to 35℃ and from pH 7.6 to pH 10.0, while that of S_(2) from 0℃ to 30℃ and from pH 8.0 to pH 9.0 and that of S_(3) from 0℃ to 35℃ and from pH 8.0 to pH 8.6. Adaptive range of S_(1) GO for temperature and pH is wider, and activity at low and high temperature and under strong acidand alkali conditions is higher than that of the imported species.