Adolescent ; Brain ; pathology ; Brain Diseases ; diagnosis ; pathology ; Child ; Diagnosis, Differential ; Female ; Granulation Tissue ; pathology ; Humans ; Magnetic Resonance Imaging ; Male ; Retrospective Studies

To construct a recombinant adenovirus containing CDglyES fusion gene, which can directly inhibit human ovarian cancer cell and indirectly inhibit vascular endothelial cell growth. Methods:We constructed prAdCDglyES using a homolo-gous recombination method in bacteria. The prAdCDglyES was transfected to 293 packaging cells using liposome, in which rAdCDgly-ES was packaged and amplified. MTT was used to observe the proliferation inhibition effect of rAdCDglyES on human ovarian cancer cells and the growth inhibition effect of expressing products of rAdCDglyES on ECV-304. Results:The titer of rAdCDglyES was 1 × 1013.3 TCID50/L, whereas the inhibition rate on human ovarian cancer cell SKOV-3 was (83.1±6.3)%. This result is significantly different from the control rAd-LacZ, which had an inhibition rate of (24.1 ± 13.2)% (P<0.01). The concentrated culture supernatant from cells transfected with rAdCDglyES can inhibit ECV-304 cell proliferation at a rate of (78.7 ± 1.6)%. This rate is significantly different com-pared with that of the control with the same concentration of culture supernatant from cells transfected with rAd-CD, with an effect on ECV-304 cell shown by an inhibition rate of (23.9 ± 9.7)%(P<0.01). Conclusion:The results showed that the recombinant adenovirus rAdCDglyES could inhibit human ovarian cancer cells directly and indirectly.

Objective To investigate the change of serum homocysteine (Hcy) and plasma fibrinogen (Fg) levels in patients with ischemic cerebrovascular diseases (ICVD) and the correlation between Hcy and Fg.Methods Blood samples were collected from 74 patients with ICVD and 40 controls. The concentrations of Hcy, folate and Fg were determinated by means of fluorescence polarization immunoassay, electrochemiluminescence, and immunoturbidimetry, respectively. In the meantime, plasma lipid was detected by routine method.Results Both Hcy and Fg levels in the patients with ICVD increased significantly as compared with the controls (all P0.05). Multivariate logistic regression analysis indicated that hypertension, high cholesterol, high Fg and high Hcy concentrations played significiant roles in ICVD.Conclusion Both hyperhomocysteinemia and hyperfibrinogenemia are independent risk factors of ICVD.

To study the application of simulated-patient teaching in practice teaching and assessment of obstetrics and gynecology,the two groups of students were taught by simulated-patient teaching and discussed cases teaching respectively,and then they were assessed by using simulated patients for clinical skills.The result shows that simulated-patient teaching is effective in improving students' clinical skills,can act as a worthy tool of clinical teaching and assessment.

ObjectiveTo develop the classification and coding of medical science data so as to merge and manage the data effectively.MethodsClassification frame was developed with linear classification, coding with matching design.ResultsThe medical science data classification frame, the contents of the frame and their code have been developed. ConclusionThe development of the classification and coding can merge and organize the medical science data effectively.

Acupuncture Points ; Drug-Related Side Effects and Adverse Reactions ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; Humans ; Seasons ; Time Factors

The ratio of plasma aldosterone concentration to plasma renin activity (ARR) is a practical parameter in screening for primary aldosteronism (PA).However,variations of the cutoff value of ARR in different studies have been reported due to plenty of influential factors that may affect the secretions of renin and aldosterone. Lack of standardization of assays for ARR also makes direct comparisons among different studies difficult.The associated influential factors on ARR were introduced in this review.

Objective To analyze the prognostic factors and trerapy strategy of lung cancer in the patients aged 80 years and over.Methods Totally 107 patients aged ≥ 80 years with lung cancer were retrospectively reviewed.Patients' clinical characteristics and treatment were analyzed.Results Median survival time of the patients was 6.9 months.92.9％ (13/14) of small cell lung cancer patients and 34.4％ (31/90) of non small cell lung cancer patients were treated.Life cycle of patients who accepted effective treatments and supportive treatments were 16.5 months and 8.7 months,respectively (P=0.008).In the early stage of tumors,survival time of patients undergoing surgery was 36.7 months,15.5 months in patients without surgery (P=0.023),while in the late stage,survival time of patients receiving combined chemotherapy was 13.4 months,4.6 months in patients receiving single agent chemotherapy(P=0.002).In small cell lung cancer,survival time of patients who received radiotherapy was 12.8 months,6.4 months in patients who did not receive radiotherapy (P=0.049).Performance status (PS),clinical stage,early surgery,late chemotherapy and radiotherapy(x2=38.236,18.831,5.187,9.827,4.186,P＜0.05),but not sex and pathology type affected the prognosis.PS score (P=0.003)and clinical stage(P=0.046) were the independent influencing factors.Conclusions Performance status and clinical stage are the independent influencing factors of lung cancer in the patients aged over 80 years.Patients may improve survival if receiving surgery,chemotherapy and/or radiotherapy when they have good PS,otherwise patients may choose best supportive care.

Objective To compare the advantages and disadvantages of cerebral angiography through the radial artery and the femoral artery approaches. Methods According to the approach way, 82 patients were divided into two groups: femoral artery group and radial artery group. After cerebral angiography the puncture time, the successful rate of puncture, the X-ray exposure time, the time of procedure and the complication of puncture site were compared between the two groups. Results In femoral artery group, the successful rate was higher and the time of puncture was shorter, but the complication was higher than that of radial artery group. Conclusions For cerebral angiography, through radial artey approach is feasible and safe better to be adopted for the elderly or the prescheduled case, but the femoral artery approach is suitable for the emergency.

Traumatic coagulopathy is a serious complication of tissue damage caused by trauma.In clinic,it easily develops into multiple system organ failure and results in high mortality.Traumatic coagulopathy is a plurality of blood coagulation disorder,its pathogenesis is extremely complex and involves tissue damage,blood coagulation,anticoagulation and fibrinolysis,inflammation and many other pathophysiological aspects.An in-depth study on the pathogenesis of traumatic coagulopathy is very important to improve clinical effect.This paper tries to do a review of recent study progress from pathophysiological mechanisms and hopes to provide reference for clinical work.