2.Report of a case with methylmalonic acidemia.
Hong-hua LIN ; Cheng WU ; Yang DONG
Chinese Journal of Pediatrics 2008;46(7):557-558
3.Effects of dendritic cells stimulated with C5b-9 on allogenic lymphocytes
Peize WU ; Shucheng HUA ; Xuemei YANG
Journal of Jilin University(Medicine Edition) 2006;0(01):-
Objective To study the effect of dendritic cells(DC) stimulated with C5b-9 on allogenic lymphocytes. Methods DC was stimulated with C5b-9.CD4+ T and CD8+ T cells were isolated by magnetic-activated cell sorting (MACS),and were cocultivated with DC.The maturation markers and cytokine secretion of T cells were analyzed by flow cytometry and ELISA.Results The high pure CD4+ T and CD8+ T cells were separated successfully.After cocultivated with DC,the muturation markers CD25 and CD69 of CD4+ T were up-regulated by 26.31% and 52.73%,and IL-2 and IFN-? of CD4+ T cells were promoted from 126.3 ng?L-1 and 156.7 ng?L-1 to 409.2 ng?L-1 and 471.5 ng?L-1;the levels of IL-10 before and after coculture were 104.3 ng?L-1 and 107.1 ng?L-1.However these markers and cytokine secretion of CD8+ T were not influenced.Conclusion C5b-9 can adjust the function of DC and regulate the immuological response of T cells.
4.Effects of electromagnetic field activated-ERK signaling pathway on proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells
Guohua YANG ; Hua WU ; Dongming ZHAO
Chinese Journal of Tissue Engineering Research 2011;15(19):3607-3610
BACKGROUND: It has been demonstrated that electromagnetic field (EMF) can adjust proliferation and differentiation of bone marrow mesenchymal stem cells, but the specific mechanism is not clear. OBJECTIVE: To investigate the effects of EMF-activated ERK1/2 pathway on proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells.METHODS: The 3rd passage of rat bone marrow mesenchymal stem cells were received EMF treatment (15 Hz, 1 mT, sine wave), 20 μmol/L PD98059 + EMF treatment, or only PD98059 treatment. Simultaneously, a normal control group was established. Western blotting was applied to detect the activation of ERK signal pathway after EMF exposure. MTT assay was used to determine the activation of proliferation of cells. And alkaline phosphatase (ALP) activity in cells was detected by an ALP kit. RESULTS AND CONCLUSION: The ERK1/2 phosphorylation, proliferation and ALP activity of rat bone marrow mesenchymal stem cells were remarkably increased after exposure to EMF (P < 0.01). PD98059 could effectively block the increasing of ERK1/2 phosphorylation and cell proliferation (P < 0.01), but elevate ALP activity in a certain level (P < 0.01). EMF stimulation can fast activate ERK1/2 signal pathway and then promote the proliferation of rat bone marrow mesenchymal stem cells, however, ERK1/2 signal pathway activation has a less effect on osteogenic differentiation of bone marrow mesenchymal stem cells.
6.Measurements of semicircular canal space direction with MRI.
Xiaokai YANG ; Shuzhi WU ; Hua YE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(19):1683-1686
OBJECTIVE:
Measure the space direction of semicircular canals to provide the anatomical basis for the diagnosis and treatment of BPPV.
METHOD:
We calculated angles among semicircular canals of 24 patients using MRI scaning with 3D-CISS sequence.
RESULT:
The angle between the left and right posterior semicircular canals was 106.61 degress ± 8.58 degrees, so the angle among the posterior semicircular canals and sagittal head plane was 53.31 degrees ± 4.29 degrees. Pairs of contralateral synergistic canal planes were not parallel, forming 171.67 degrees ± 4.36 degrees between the left and right horizontal semicircular canal planes, 154.37 degrees ± 10.87 degrees between the left posterior and right anterior semicircular canal planes and 156.84 degrees ± 9.34 degrees between the right posterior and left anterior semicircular canal planes.
CONCLUSION
Our measurement of the angles among semicircular canals coincided with those of previous reports. The angles between contralateral synergistic canal planes were close to parallel, but the angle between the posterior semicircular canals and sagittal head plane was great than 45 degrees that traditionally thought to be.
Humans
;
Magnetic Resonance Imaging
;
Semicircular Canals
;
anatomy & histology
7.Expression of RASIP1 in hepatocellular carcinoma and its role in suppressing invasion
Dipeng OU ; Defu WU ; Hao YANG ; Dong HUA ; Lianyue YANG
Chinese Journal of Hepatobiliary Surgery 2014;20(5):342-345
Objective To explore the expression levels of RAS-interacting protein 1 (RASIP1) mRNA and protein in hepatocellular carcinoma (HCC) tissues and its cell lines,and to analyze the relationship between RASIP1 and tumorigenesis and progression of hepatocellular carcinoma.Methods The expression levels of RASIP1 mRNA and protein in 29 hepatocellular carcinoma tissues and the corresponding adjacent non-cancer liver tissues (ANLTs),as well as those in the HCC cell lines such as LO2,HEPG2,MHCC97-H and HCCLM3 were detected using real-time PCR and western blot.Results The RASIP1 expression levels decreased significantly in HCC tissues when compared with the corresponding ANLTs; The expression levels of RASIP1 mRNA and protein in LO2 were significantly higher than those in other HCC cell lines (P < 0.05) ; The expression levels of RASIP1 mRNA and protein in MHCC97-H and HCCLM3 were significantly lower than those in HepG2 (P < 0.05).Conclusions HCC tissues had lower expression than those in ANLTs.On analyzing the RASIP1 levels of HCC tissues and its cell lines,we speculated that RASIP1 might suppress recurrence and metastasis of HCC.
8.Study on the HPLC Fingerprint of Blumea balsamifera and Its Fake B. riparia
Hua FENG ; Ye YANG ; Xiangpei WANG ; Hongmei WU ; Xianyou YANG
China Pharmacy 2017;28(9):1257-1261
OBJECTIVE:To establish the HPLC fingerprint for Blumea balsamifera and its fake B. riparia. METHODS:HPLC was performed on the column of Uitimate-C18 with mobile phase of acetonitrile-0.05% phosphoric acid(gradient elution)at a flow rate of 0.6 mL/min,detection wavelength was 270 nm,column temperature was 25 ℃,and injection volume was 7 μL. Using quercetin as a reference,Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine(2004 A edition)was used for the common peaks identification and similarity analysis of 16 batches of B. balsamifera and 5 batches of B. ri-paria. RESULTS:There were 61 common peaks in the 16 batches of B. balsamifera,similarity degree was 0.931-0.995,which was higher than the similarity degree of 5 batches of B. riparia. CONCLUSIONS:The established fingerprint can provide reference for the identification and quality evaluation of B. balsamifera.
10.Comparative study of laparoscopic and endoscopic cooperative surgery for elderly patients with gastric gastrointestinal stromal tumor
Hua YANG ; Gang XIAO ; Xinping ZHOU ; Guoju WU ; Gang ZHAO
Chinese Journal of Geriatrics 2014;33(9):973-975
Objective To investigate the feasibility,safety and efficacy of laparoscopic and endoscopic cooperative surgery (LECS) for elderly patients with gastric gastrointestinal stromal tumor (GIST).Methods 54 cases with GIST aged 65 years and over in Department of Gastrointestinal Surgery in Beijing Hospital from Dec.2008 to Dec.2012 were selected.31 cases underwent LECS and 23 cases underwent open surgery.Clinical data including operation time,intraoperative blood loss,complications,tumor size,length of incision,postoperative gastrointestinal function recovery time,postoperative in hospitalization and follow up data were retrospectively analyzed in patients.Results There were 17 males and 14 females in the LECS group,and the mean age was (72.8±5.9)years.29 patients underwent laparoscopic-assisted partial gastrectomy,and 2 cases underwent laparoscopicassisted endoscopic dissection in LECS group.All operations were successful with no conversion to open surgery and death.There were 13 males and 10 females in the open surgery group,and the mean age was(73.3±6.1)years.The mean operation time was longer in LECS group than in open surgery group [(120.6±32.8) min vs.(86.3±33.5) min].The mean intraoperative blood loss,mean tumor size,mean length of incision,mean time of gastrointestinal function recovery,mean postoperative hospitalization were less or smaller in LECS group than in open surgery group [(40±23.4) ml vs.(130±65.6) ml,(2.3±1.2)cm vs.(3.6 ±1.8)cm,(3.6±1.1) cm vs.(14.4±3.5) cm,(47.7± 10.4)h vs.(61.4±11.9)h,(5.1±2.3)d vs.(7.2±2.5)d,respectively,t=3.192,1.831,5.212,2.014,3.519,P=0.002,0.012,0.000,0.015,0.001].According postoperative tumor risk assessment,13 cases were at very low risk,15 cases at low risk and 3 cases at middle risk in LECS group; 4 cases were at very low risk,14 cases at low risk and 5 cases at middle risk in open surgery group.The risk degree was lower in LECS group than in open surgery group (x2 =5.63,P=0.017).During a follow-up of 5 53 months,death without GIST was found in 5 patients in LECS group and 4 cases in open surgery group.Hepatic metastasis was found in 1 case in open surgery group.Conclusions LECS is a safe and feasibility alternative approach for elderly patients with gastric GISTs.It has more minimal invasion,fast recovery and satisfaction with short term outcomes as compared with conventional open surgery.