1.Estimation on ethanol content measured in vivo.
De-quan ZHU ; Hua PANG ; Jin-rong LI
Journal of Forensic Medicine 2006;22(1):S4-7
Recently the cases after drinking are increasing, but the systematic studys on ethanol content in vivo and correlative problems are still absent. According to the measured results of ethanol content in vivo, ethanol metabolic distributed rules, mechanisms of ethanol toxicological effect and its production in vivo, this study analysed systematically the time after drinking, total quantity of absorbed ethanol, psychological situations, behavioral dominated ability, death causes and manners in order to find out the implied forensic medical information and provide the reference for colleague.
Alcohol Drinking/metabolism*
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Alcohol-Induced Disorders
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Ethanol/metabolism*
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Forensic Medicine
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Humans
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Postmortem Changes
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Substance Abuse Detection/methods*
;
Time Factors
2.Intraluminal enucleation in transurethral vaporization and electro-resection of the prostate
Zhi-Jian YANG ; Zhao-Hua LIU ; Min-Jian CHEN ; Ming-Nian YU ; Cheng PANG ; Jin-Jun MO ; Xi-Hai LIU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(09):-
Objective To investigate the clinical efficacy of intraluminal enucleation in transurethrat vapor- ization and electro-reesection of the prostate.Methods A retrospective analysis was reviewed in 62 case of prostatic hypertrophy,which were treated with intraluminal enucleation in vaporization of prostate.All pacients had a sucessful operation.There were 12 case in unipolar vaporization and 50 in plasmakenitic bipolar vaporization.Results Opera- tion time ranged from 50 to 162 minutes,with an average of 76min.Bleeding ranged from 40 to 200 ml,with an av- erage of 110ml.There was no blood transfusion.The weight of prostate was 62~138g,the catheter was maintained for 3~5 days postoperatively.The hospital stay was 7~10 days,average 8 days.All patients were cured.There was a fllow-up for 1~20 months,with an average of 8 months.The IPSS decreased by 22 points on average,and peak urine flow(Qmax)increasd to(16.8?3.3)ml/s.There wre no urethralstricture,permanent urinary incontinence, TURS,postoperative hemorrhage,retrograde ejaculation and recurrence.Conclusions Intraluminal enucleation in treatment of prostalic hypertroply is a new,safe,and effective method,which should be popularized in clinical prac- tice.
3.Effect of activation of cellular immunity on p58+ cells expressing killer-cell-inhibitory receptor cells.
Xing-Hua PANG ; Rong-Qing PANG ; Kun-Yuan GUO ; Jiu-Gang SONG ; Jiang-Qi LI ; Yu-Jin ZHANG ; Xiao-Fen YANG
Journal of Experimental Hematology 2003;11(1):70-73
UNLABELLEDThe purpose of this study was to evaluate the effects of cellular immunity activation on P58(+) cells expressing killer cell inhibitory receptor (KIR) and their regulatory function on cellular immunity, and provid theoretical data for preventing graft-vers-host disease (GVHD) in stem cell transplantation therapy. The mononuclear cells from human peripheral blood were incubated with IL-2, Con A and Lipostin (LP) for 72 hours. The KIR expressing cells, P58.1(+) and P58.2(+) cells, were analyzed by flow cytometry. The results showed that the percentages of CD3(+), CD4(+), CD8(+), CD16(+)CD56(+), P58.1(+) and P58.2(+) cells were greatly increased after treated with IL-2, Con A and LP, separately or in combination, and the percentages of above cells in combined treatment groups were higher than those of single stimulated groups, especially the percentage of cells in the IL-2 + LP group was significantly higher than those in IL-2 and LP singly treated groups.
IN CONCLUSIONIL-2, Con A and LP possess the ability to induce the expression of KIR and stimulate proliferation of P58.1(+) and P58.2(+) cells while to activate the celluar immunity response, the expression of P58 gene may be regulated by the activation of cellular immunity.
Adult ; CD3 Complex ; analysis ; CD4 Antigens ; analysis ; CD56 Antigen ; analysis ; CD8 Antigens ; analysis ; Cell Count ; Cell Division ; drug effects ; Concanavalin A ; pharmacology ; Flow Cytometry ; Humans ; Interleukin-2 ; pharmacology ; Leukocytes, Mononuclear ; cytology ; drug effects ; immunology ; Receptors, IgG ; analysis ; Receptors, Immunologic ; analysis ; Receptors, KIR ; Receptors, KIR2DL3
4.Thread-moxa in Zhuang folk medicine combined with acupuncture and external application drugs on AIDS patients with herpes zoster: a clinical observation.
Zhen-wei LIU ; Jin-hua MO ; Jun PANG ; Xin DENG
Chinese Journal of Integrated Traditional and Western Medicine 2013;33(8):1050-1053
OBJECTIVETo observe the efficacy of thread-moxa in Zhuang folk medicine (TM) combined with acupuncture and external application drugs for AIDS patients with herpes zoster (AHZ).
METHODSA randomized, controlled clinical trial was conducted in 60 patients with AHZ. They were randomly assigned to the treatment group (treated with TM combined with acupuncture and Jingwanhong Scald Ointment) and the control group (treated with Famciclovir Tablet, nimesulide dispersible tablet, vitamin B1, ribavirin ointment). The treatment course was 14 days for both groups.The clinical efficacy, significant efficiency visual analog scale score (VAS), sleep quality score (QS), the postherpetic neuralgia rate in 1 year after treatment were observed.
RESULTSThe markedly effective rate was significantly higher in the treatment group than in the control group (86.7% vs. 53.3%, P < 0.01). There was no statistical difference in the total effective rate between the two groups (96.7% vs. 80.0%, P > 0.05). The post-treatment VAS, QS, the time for pain disappearance, skin repair, crusting, and 1-year postherpetic neuralgia incidence rate were significantly lower in the treatment group than in the control group (P < 0. 05, P < 0.01).
CONCLUSIONSTM combined with acupuncture and Jingwanhong Scald Ointment was effective for treating AHZ patients. It relieved pain quickly, shortened their course of disease, and improved their quality of sleep.
Acquired Immunodeficiency Syndrome ; complications ; therapy ; Acupuncture Therapy ; Adult ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Herpes Zoster ; complications ; therapy ; Humans ; Male ; Middle Aged ; Pain Management ; Phytotherapy ; Sleep ; Treatment Outcome ; Young Adult
5.Association of vitamin D receptor gene polymorphisms with the susceptibility to chronic periodontitis of Han nationality.
Jin-cai ZHANG ; Hua-ou GENG ; Wen-bo MA ; Ping HUANG ; Ru-yu PANG ; Yun-hui ZHANG
Chinese Journal of Stomatology 2005;40(1):50-53
OBJECTIVETo investigate association of vitamin D receptor (VDR) gene polymorphisms with the susceptibility to chronic periodontitis (CP) of Han Nationality.
METHODSBuccal swabs from 166 patients with severe, moderate and mild CP respectively and 80 matched control individuals were collected. DNA was extracted from these buccal swabs using Chelex-100 method. VDR BsmI, ApaI, TaqI were tested with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The distribution of the genotypes and allele frequencies in the patient and control groups were analyzed.
RESULTSThe frequency of VDR ApaI allele A was significantly higher among patients with CP than controls. Frequencies of VDR ApaI allele A were significantly higher in severe CP patients than in moderate CP and mild CP respectively. There was no significant difference in the genotype distribution or the allele frequencies of VDR BsmI and TaqI between the controls and CP patients.
CONCLUSIONSThese data indicate that VDR ApaI allele A may be related to the susceptibility to CP in Han Nationality.
Adult ; Aged ; Alleles ; Asian Continental Ancestry Group ; genetics ; Chronic Periodontitis ; genetics ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Receptors, Calcitriol ; genetics
6.Diagnosis and percutaneous interventional management of hepatic venous outflow obstruction after adult-to-adult living donor liver transplantation: Two cases report and literatures review
Zaibo JIANG ; Mingan LI ; Jiesheng QIAN ; Pengfei PANG ; Zhengran LI ; Jin WANG ; Hong SHAN ; Mingsheng HUANG ; Hua LI ; Shuhong YI ; Kangshun ZHU ; Shouhai GUAN
Chinese Journal of Interventional Imaging and Therapy 2010;7(1):27-30
Hepatic venous outflow obstruction is a severe complication after liver transplantation, often occurs after living donor liver transplantation (LDLT). In this article, the clinical and imaging data of two patients with hepatic venous outflow obstruction after LDLT were analyzed retrospectively, and the related literatures were reviewed to explore the diagnosis and the interventional therapy of this complication. Hepatic venous outflow obstruction can be confirmed with percutaneous transhepatic venography. Percutaneous interventional managements, including balloon angioplasty and stent implantation are safe, easy and effective for the treatment of hepatic venous outflow obstruction after adult-to-adult living donor liver transplantation (A-A LDLT).
7.Effect of intracellular acidification on drug resistance of leukemia cells with high P-glycoprotein expression.
Qing-hua LI ; Ying LU ; Wei-na JIN ; Ya-ni LIN ; Rong-hua HU ; Xiao-fan ZHU ; Jian-xiang WANG ; Tian-xiang PANG
Chinese Journal of Hematology 2009;30(9):605-609
OBJECTIVETo investigate the impact of intracellular acidification (IA) on drug resistance of leukemia cells with high P-glycoprotein (P-gp) expression, and to provide a new method for the reversing of multidrug resistance (MDR).
METHODSReal-time PCR was used to determine the expression level of mdr1 gene, and the leukemia cells with high P-gp expression were selected. The specific inhibitor of Na+/H+ exchanger 1 and the "high K+" buffer were used to acidify the cells, and the confocal laser microscopy was used to determine the intracellular pH (pHi) and effect of IA on the accumulation of doxorubicin. The MTT method was used to determine the effect of IA on the cell viability. The flow cytometry was used to detect the effect of IA on the P-gp function, and Western blotting was used to determine the effect of IA on the expression of P-gp.
RESULTSThe pHi was decreased to 7.0, and compared with that of control the mdr1 mRNA expression was decreased to (53.2+/-11.0)% after 1 h, and to (16.6+/-7.0)% after 3 h treatment. The P-gp expression was decreased to (56.0+/-9.0)% of the control after 3 h treatment. The accumulation of Rh123 was 71.03+/-0.47 at pHi 7.0, which was increased obviously as compared to the control group 20.07+/-0.39. The increased accumulation of doxorubicin was also observed by confocal laser microscopy.
CONCLUSIONThe expression and function of P-gp on the patients cells are inhibited by IA.
ATP Binding Cassette Transporter, Sub-Family B ; ATP-Binding Cassette, Sub-Family B, Member 1 ; genetics ; metabolism ; Cell Survival ; drug effects ; Doxorubicin ; pharmacokinetics ; Drug Resistance, Neoplasm ; drug effects ; Guanidines ; pharmacology ; Humans ; Hydrogen-Ion Concentration ; drug effects ; Leukemia ; drug therapy ; metabolism ; RNA, Messenger ; genetics ; Sulfones ; pharmacology ; Tumor Cells, Cultured
8.Comparison of effect between homologous recombinant gene knockout and siRNA gene silence in cell lines.
Qing-Hua LI ; Wei-Na JIN ; Hua-Mei ZHANG ; Yong-Xin RU ; Tian-Xiang PANG
Journal of Experimental Hematology 2010;18(1):122-126
The objective of this study was to compare the effects between knocking-out Sam68 gene by homologous recombination method and silencing the gene by siRNA silencing technique in DT40 cell line. Gene targeting technique was used to isolate Sam68 gene-deleted chicken DT40 cells. Meanwhile, Sam68 gene silencing cells was obtained by using stable expression of siRNA plasmid pSilencer-Sam68. Then, the function of these two cell lines were analyzed by comparing with wild-type DT40 cell line. The results showed that the growth retardation in Sam68 gene knocked-out cell line was observed due to elongation of the G2/M phase, but which could not be found in Sam68 gene silencing cell line. It is concluded that in accordance with study of protein function in living cells, use of gene knockout technique for cell line can provide the experimental results more real than those resulting from gene silence technique.
Animals
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Cell Line, Tumor
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Chickens
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Gene Expression Regulation, Neoplastic
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Gene Knockout Techniques
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Gene Silencing
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Gene Targeting
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Plasmids
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RNA, Small Interfering
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genetics
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Transfection
9.Increasing sensitivity of leukemia cells to imatinib by inhibiting NHE1 and p38MAPK signaling pathway.
Rong-Hua HU ; Wei-Na JIN ; Guo-Qiang CHANG ; Ya-Ni LIN ; Jian WANG ; Yong-Xin RU ; Qing-Hua LI ; Tian-Xiang PANG
Journal of Experimental Hematology 2012;20(6):1341-1345
This study was aimed to investigate whether the inhibition of NHE1 activity and intracellular acidification can reverse resistance of leukemia cells to the imatinib and to explore downstream signal molecule networks of BCR/ABL in the cells of chronic myelocytic leukemia (CML) patients. The mRNA and protein expression of P-glycoprotein (Pgp) and the drug accumulation were assayed after acidifying the primary leukemia cells of patients or K562/DOX and K562/G01 cells. The effects of intracellular acidification of primary leukemia cells on the phosphorylation level changes of ERK1/2 and p38 MAPK were analyzed by Western blot. The results showed that the intracellular concentration of drugs in the advanced patients increased and the sensitivity of K562/DOX and K562/G01 cells to imatinib was enhanced after intracellular acidification or treatment with NHE1 inhibitor cariporide. With downregulation of intracellular pH, the phosphorylation of p38 MAPK decreased in advanced patients and the phosphorylation of ERK1/2 increased within 3 min and then decreased after 30 min. SB203580, the specific inhibitor of p38 MAPK, displayed a synergistic effect with the inhibitor of NHE1 to downregulate the mRNA and protein expression of Pgp. It is concluded that the inhibiton of NHE1 can significantly decrease the protein expression of Pgp in K562/DOX and K562/G01 cells, increase the accumulation of Rhodamine123 and doxorubicin in the cells of advanced patients and enhance the sensitivity of cells to imatinib in which the p38 MAPK signal transduction pathways involves.
ATP-Binding Cassette, Sub-Family B, Member 1
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metabolism
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Benzamides
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pharmacology
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Cation Transport Proteins
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antagonists & inhibitors
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metabolism
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Drug Resistance, Neoplasm
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drug effects
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Enzyme Inhibitors
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pharmacology
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Gene Expression Regulation, Leukemic
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Humans
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Imatinib Mesylate
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Imidazoles
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pharmacology
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K562 Cells
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MAP Kinase Signaling System
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Piperazines
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pharmacology
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Pyridines
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pharmacology
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Pyrimidines
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pharmacology
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Sodium-Hydrogen Exchanger 1
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Sodium-Hydrogen Exchangers
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antagonists & inhibitors
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metabolism
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p38 Mitogen-Activated Protein Kinases
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antagonists & inhibitors
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metabolism
10.Orthogonal design based optimization of a mouse model of acute liver failure induced by D-galactosamine and lipopolysaccharide.
Hao-zhen YANG ; Long CHEN ; Jing-jing TONG ; Hui-ying ZHANG ; Fei PANG ; Zhi-heng XU ; Shao-jie XIN ; Jin-hua HU
Chinese Journal of Hepatology 2013;21(6):464-466
OBJECTIVETo apply an orthogonal design optimization strategy to a mouse model of acute liver failure induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS) exposure.
METHODSA four-level orthogonal array design (L16(45)) was constructed to test factors with potential impact on successful establishment of the model (D-GalN and LPS dosages, and dilution rate of the D-GalN/LPS mixture). The mortality rate of mice within 24 hours of D-GalN/LPS administration was determined by the Kaplan-Meier method. The model outcome was verified by changes in serum alanine transferase level, liver histology, and hepatocyte apoptosis.
RESULTSThe orthogonal array identified the optimal model technique as intraperitoneal injection of a combination of D-GalN and LPS at dosages of 350 mg/kg and 30 mug/kg, respectively, and using a dilution rate of 3. The dosages tested had no effect on survival. The typical signs of liver failure appeared at 6 hrs after administration of the D-GalN/LPS combination.
CONCLUSIONThe orthogonal design optimization strategy provided a procedure for establishing a mouse model of acute liver failure induced by D-GalN and LPS that showed appropriate disease outcome and survival, and which will serve to improve future experimental research of acute liver failure.
Animals ; Apoptosis ; Disease Models, Animal ; Galactosamine ; adverse effects ; Lipopolysaccharides ; adverse effects ; Liver Failure, Acute ; chemically induced ; Male ; Mice ; Mice, Inbred C57BL