Disease Outbreaks ; Humans ; Influenza A Virus, H1N1 Subtype ; Influenza, Human ; epidemiology ; virology

Objective: To investigate the expression and clinical significance of Ezrin, a tumor metastasis related gene, in tissues of nasopharyngeal carcinoma (NPC). Methods: The expression of Ezrin was detected with two-step immunohistochemistry technique in tissues of 38 NPC patients and 20 nasopharyngitis patients. All of the Ezrin expression and clinical data (gender, age, clinical stage, recurrence, and ]ymphonode metastasis) were compared and analyzed between two groups. Resuits: The Ezrin expressions were significantly different between NPC tissue (and peri-NPC tissue) and naeopharyngitis tissue (P＜0.05). No significant difference was found in the Ezrin expressions between the NPC tissue and the peri-NPC tissue (P＞0.05). It was found by Spearman analysis method that the Ezrin expression increased with patients' age (P＜0.05), but no significam relationship with gender, clinical stage, recurrence, and lymphonode metastasis (P＞0.05). The Ezrin expression influenced the survival time of NPC patients significantly (P＜0.01). Conclusion: The Ezrin expression can be an useful prognostic factor for NPC patients.

Adult ; Heavy Metal Poisoning ; Hemoperfusion ; Humans ; Male ; Poisoning ; therapy ; Thallium ; poisoning

OBJECTIVE:To study the quality standards for Shenchang injection.METHODS:TLC was applied to identify the properties of Borneol and volatile oil of Rhizoma Acori Tatarinowii in the formula.HPLC was adopted to determine the content of ?-asarone in Shenchang injection.RESULTS:?-asarone showed good linear relationship in the range of 0.632 3～ 1.264 6? g.The average recovery of ammonium ?-asarone was 99.92%(RSD=0.91%).CONCLUSION:The standard can be used for the quality control of Shenchang injection.

Objective To investigate the effect of radical surgical excision of total cyst for hepatic cystic echinoccosis. Methods During six years,86 patients with hepatic cystic echinococcosis were treated by radical surgical excision of the total cyst.Hospital stay,abdominal drainage time,residual cavity drainage,accumulated liquid in residual cavity,residual cavity infection and recurrence were compared with that in 90 cases receiving internal capsule extirpation. Results Radical surgical excision of total cyst for hepatic cystic echinococcosis is safe and very effective,the average hospital stay was significant shorter(P＜0.001)and there was no significant complications such as billiay fistula,residual cavity infection,accumulated liquid of residual cavity and recurrence of hydatid cyst. Conclusion Radical surgical excision of total cyst is an ideal and new therapeutic method for the treatment of hepatic cystic eehinococcosis.

Objective To investigate the prevalence rate of complications in children with cerebral palsy (CP).Methods 80 CP children were recruited.Their parents were interviewed for high risk factors and feeding his- tory.Each case was assessed neurologically for type of CP and diagnosed for complications.And all cases were detect- ed by magnetic resonance imaging(MRI)of the brain.Results Among 80 patients,60 cases(75%)suffered from complications,the majority patients had difficulty in taking in food,intelligence retardation,hearing impediment and vision impediment.The prevalence rate of complications with mixed CP was 85 %.MRI abnormalities of the brain were accounted for 74.5 % and periventrieular leakomalacia(PVL)was more common(45.1%).Conclusion This study suggested timely rehabiliatation and interventions must be started to significantly improve the levels of intelli- gence.And the epilepsy and nonepileptic events must be identified to avoid mistakes in diagnosis.

Animals ; Brain ; metabolism ; physiology ; Diet ; Glucose Transporter Type 1 ; genetics ; metabolism ; Glucose Transporter Type 4 ; genetics ; metabolism ; Male ; Mice ; Mice, Inbred ICR ; Physical Conditioning, Animal ; physiology ; RNA, Messenger ; genetics ; metabolism ; Random Allocation

Objective To evaluate measures in the prevention of pancreatic fistula after distal pancreatectomy.Methods Clinical data of 124 cases of distal pancreatectomy performed during 2000 and 2009 in our hospital were retrospectively analyzed,including 86 cases of pancreatic carcinoma,12 cases of pancreatic pseudocyst and pancreatic benign tumor,18 cases of gastroenteric tumor with pancreatic body/tail invasion,3 cases of chronic pancreatitis with body/tail pseudocyst,5 cases of pancreatic injury.Cases were divided into two groups retrospectively by ways the stump pancreas were closed.Group A included 63 cases in which the stump end was closed by simple interrupted mattress suture,group B including 61 cases in those the stump was sutured by interrupted mattress combined with a safe ligation of the main pancreatic duct.Results Pancreatic fistula occurred in 22 cases in group A,the incidence was 34.9％,among the 22 cases,10 cases were high-flow type,12 cases were low-flow type; while the pancreatic fistula occurred in 9 cases in group B,the incidence was 14.7％,there was no high-flow type fistula.Conclusions Interrupted mattress suture combined with interlocking suture and suture of main pancreatic duct is effective in decreasing the rate of pancreatic fistula after distal pancreatectomy.

This list of clincal data management documentation is to ensure standardized and adequate archival of trial documents and records in clinical data management, which is applicable to all of phase I-IV clinical trials.
Clinical Trials as Topic ; Data Collection ; standards ; Documentation ; standards

Objective To compare the therapeutic effect of inhaled aerosolized and intravenous milrinone,a phosphodiesterase-3 inhibitor in rats with oleic acid-induced acute lung injury (ALI) .Methods Forty male SD rats weighing 300-350 g were randomly divided into 4 groups (n=10 each) : group Ⅰ normal control: group Ⅱ ALI; group Ⅲ milfinone inhalation and group Ⅳ intravenous milrinone.The animals were anesthetized with intraperitoneal 2% pentobarbital 40 mg/kg,tracheostomized and mechanically ventilated (FiO2 30%,VT 10 ml/kg,RR 80 bpm,I:E=1:2).The chest was opened and the heart was exposed.Pulmonary artery was catheterized via fight ventricle.MAP,CVP,airway pressure and pulmonary artery pressure (PAP) were monitored.ALI was induced with 10% oleic acid 2 ml/kg administered through fight external jugular vein in group Ⅱ,Ⅲ and Ⅳ.In control group 0.1% BSA solution 2 ml/kg was administered iv instead of oleic acid.In group Ⅲ at 30 min after oleic acid administration aerosolized milrinone 1 mg/ml was inhaled 4 times at 60 min interval.Each time milrinone was inhaled for 10 min.In group Ⅳ at 30 min after oleic acid administration a bolus of 10 μg/kg milrinone was given iv followed by 10 min milrinone infusion at 1 μ·kg-1·min-1.The same procedure was repeated 4 times at 60 min interval.MAP and PAP were recorded and blood samples were taken from carotid artery and pulmonary artery for blood gas analysis at the 1st,2nd,3rd and 4th treatment.PaO2/FiO2 and Qs/Qt were calculated.The animals were sacrificed by exsanguination after the 4th treatment.The lungs were removed.The left lung was lavaged.Neutrophil count and protein content in broncho-alveolar lavage fluid (BALF) were determined.W/D lung weight ratio and lung myeloperoxidase (MPO) activity were measured.The uhrastructure of the lung was examined with electron microscope.Results The MAP was significantly lower after oleic acid adminstration in group Ⅳ than in other 3 groups.PaO2/FiO2 was significantly decreased and Qs/Qt increased by iv oleic acid in group Ⅱ ,Ⅲ and Ⅳ.PAP was significantly increased after iv oleic acid in group Ⅱ ,Ⅲ and Ⅳ but was significantly lower in group Ⅲ and Ⅳ than in group Ⅱ .The neutrophil count and total protein content in BALF,W/D ratio and lung MPO activity were significantly increased in group Ⅱ ,Ⅲ and Ⅳ as compared with control group(Ⅰ) and were significantly higher in group Ⅳ than in group Ⅲ.The lung damage induced by oleic acid was less serious in group Ⅲ and Ⅳ than in group Ⅱ .Conclusion Inhaled aerosoLized milrinone has better therapeutic effect than intravenous milrinone in rats with oleic acid-induced ALI and is safer.