Audiology ; China ; Congresses as Topic

Objective To analyze the sandtray topics of the children with behavior problems according to the grounded theory method.Methods Collected and described children' s sandtray topics of different types of behavior problems,age and gender.And analyzed them base on a 3 levels coding list compiling according to the ground theory method.Results The frequency of “negative theme” (3.33 ± 1.25 ) was higher than “positive theme” (0.81 ± 1.25 ) with significant difference (P＜0.01) in feature level 1.Age factor had significant effect upon the frequency of “positive theme” in feature level 1 (P ＜ 0.05 ).And also had impact on “harmony”,“ development” in features level 2 (F =3.039,P ＜ 0.05 ).Gender factor,interaction of gender and age had significant effects on frequency of “negative theme” in feature level 1 (F =5.395,P ＜ 0.05 ; F =4.222,P ＜ 0.05 ).Conclusion The characteristics of the children' s sandtay with behavioral problems mainly are “negative theme”.And it shows different features in children with different gender or age.

Objective To investigate the application of theoretical K value and the calibration K value on Hitachi 7600 Automatic Biochemical Analyzer .Methods Rate method was employed to detect alanine aminotransferase (ALT ) ,aspartate aminotransferase (AST) ,gamma-glutamyl transpeptidase(γ-GT) ,creatine kinase(CK) and lactate dehydrogenase(LDH) .The theoretical K value and K values calibrated by two kinds of calibration serum were recorded as K ,K1 ,K2 and the three kinds of calibration results of enzymes mentioned above were compared .Results The theoretical K values of ALT ,AST ,γ-GT ,CK and LDH were all less than their two kinds of actual K values .The results of the calibration K value were significantly superior to that of the theoretical K value .The calibration results R2 value of five kinds of enzymes were all greater than 0 .95 .Conclusion The theoretical K value is not suitable for clinical laboratories ,and the two kinds of calibration serum can meet the needs of quality control and clinical work .

The study of subjcts with T2DM (n=120) and NGT (n=106) in using the screening of PGC-1? gene for SNPs by PCR-SSCP and DNA sequencing showed that four SNPs(Thr394Thr、Gly482Ser、Thr528Thr、Thr612Met) in the PGC-1? gene were found in 50 Han Chinese type 2 diabetic patients.A Gly482Ser polymorphism of PGC-1? gene was associated with type 2 diabetes.[

Audiology ; China ; Congresses as Topic

Behcet Syndrome ; complications ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; complications

Animals ; Catechin ; analogs & derivatives ; pharmacology ; Disease Models, Animal ; Fatigue ; metabolism ; physiopathology ; Learning ; drug effects ; Male ; Memory ; drug effects ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate ; metabolism

AIM: Hydroxymethylglutaryl CoA (HMG-CoA) reductase inhibitors, such as simvastatin, have been shown to reduce atherosclerotic cardiovascular morbidity and mortality by mechanisms unrelated to its lipid-lowering effect. Several studies have shown that simvastatin induces apoptosis in a varieties of cell lines including vascular smooth muscle cells (VSMC). The aim of this study was to investigate the signal pathways involved in apoptosis induced by simvastatin. METHODS: Cultured VSMC were treated with simvastatin. Calpain activity was determined by measuring Ca 2+ ionophore-specific calpain substrate (suc-LLVY-AMC), caspase-3 activation was detected by Western blot, and apoptotic changes were distinguished by annexin Ⅴ binding and DNA laddering. RESULTS: After incubated with 30 ?mol/L simvastatin for 8 h, calpain activity had a marked increase ( P

Objective:To determine the content of notoginsenoside R1 , ginsenoside Rg1 and Rb1 in Shuxuening capsules. Meth-ods:The three constituents were determined on a Hypersil ODS-2 C18 column (250 mm × 4. 6 mm, 5 μm) with gradient elution using acetonitrile (A) -aqueous solution (B) (0-8 min, 20%A→20%A, 8-40 min, 20%A→30%A, 40-60 min, 30%A→45%A) at the detection wavelength of 203 nm with a flow rate of 1. 0 ml·min-1 . The column temperature was 25℃ and the injection volume was 20μl. Results:The calibration curve showed good linearity within the concentration range of 0. 05-0. 50 mg·ml-1 for notogisenoside R1 , and 0.20-2.00 mg·ml-1 for ginsenoside Rg1 and Rb1(r =0.999 9). The average relative recovery was 98.79%, 98.42% and 98. 89% for each constituent(RSD=0. 85%, 0. 97% and 0. 74%, respectively, n=6). The intra-day RSD was 0. 49%, 0. 20% and 0. 39%, and the inter-day RSD was 0. 75%, 0. 56% and 0. 51%, respectively. The RSDs of stability test and repeatability test were less than 1%. Conclusion:The method is simple with good accuracy and repeatability, which can be used for the determination of no-toginsenoside R1 , ginsenoside Rg1 and Rb1 in Shuxuening capsules.

Objective To discuss the impact on serum interleukin-4 and interleukin-17 in patients with hypertension and early renal damage treated with intermediate frequency If pulse electrical stimulation on acupuncture points. Methods Selected 100 patients diagnosed with early renal damage in hypertension, those were divided into control group and treatment group randomly,50 patients in the control group treated with conventional antihypertensive drugs and symptomatic treatment of diseases, while the treatment group on the basis of conventional therapy plus IF pulse point therapy,20 days after treatment compared serum IL-4 and IL-17 levels. Results Treatment group,serum IL-14 and IL-17 levels were significantly lower than the control group (all P ＜ 0.05). Conclusion Shenshu, Mingmen ,Guanyuan and other points by electrical stimulation pulse frequency ,could make IL-4 ,IL-17 and other indicators of early renal damage improve the role, and reduce hypertension early target organ damage, and help reduce pressure and reduce the amount of antihypertensive drugs.