OBJECTIVE:To provide references for the revision of pharmaceutical talents training plan in higher vocational colleges. METHODS:Selecting parts of freshmen,sophomores and junior students majoring in pharmacy in our hospital as subjects, questionnaires were conducted for the effect of hospital“pharmacy trusteeship”policy on their employment selection,and the results were statistically analyzed. RESULTS:Totally 190,390,320 questionnaires were received from junior students,sophomores and freshmen. The surveyed students knew less about“pharmacy trusteeship”policy,only a maximum by junior students of 35.79%(comparison of different grades P<0.05);while among the surveyed students from different career directions,the highest awareness rate about“pharmacy trusteeship”policy was from the students whose hospital pharmacy(41.30%-45.21%)(comparison of different career direction P>0.05). The career direction of hospital pharmacy had the highest proportion of surveyed students in each grade(38.42%-53.13%),especially the freshmen(comparison of different grades P<0.05,comparison of different career direction in a same grade P<0.05). But after knowing the policy,the surveyed students had the lowest recognition for the original career direction,especially the junior students(64.38%)(comparison of different career direction P<0.05). The surveyed freshmen and sophomores were firstly concerned the stability and then the welfare and income of the job,the surveyed junior students were firstly concerned the welfare and income and then authorized strength and the stability of the job. CONCLUSIONS:The pharmacy trusteeship policy has not yet been fully implemented,its effect on employment selection of higher vocational students majoring in pharmacy is relatively backward and the deeper effects have not been appeared completely.

Despite decades of research,cancer continues to be a major global health concern.The human mouth appears to be a multiplicity of local environments communicating with other organs and causing diseases via microbes.Nowadays,the role of oral microbes in the development and progression of cancer has received increasing scrutiny.At the same time,bioengineering technology and nanotechnology is growing rapidly,in which the physiological activities of natural bacteria are modified to improve the therapeutic efficiency of cancers.These engineered bacteria were transformed to achieve directed genetic reprogramming,selective functional reorganization and precise control.In contrast to endotoxins produced by typical genetically modified bacteria,oral flora exhibits favorable biosafety characteristics.To outline the current cognitions upon oral microbes,engineered microbes and human cancers,related literatures were searched and reviewed based on the PubMed database.We focused on a number of oral microbes and related mechanisms associated with the tumor microenvironment,which involve in cancer occurrence and development.Whether engineering oral bacteria can be a possible application of cancer therapy is worth consideration.A deeper understanding of the relationship between engineered oral bacteria and cancer therapy may enhance our knowledge of tumor pathogenesis thus providing new insights and strategies for cancer prevention and treatment.

Objective: To explore the status of medication adherence among patients with depressive disorder and analyze its potential influencing factors. Methods: A total of 406 patients with depression who visited our hospital from January 2015 to December 2017 were selected as subjects of study. General data of patients were collected at the time of consultation, and drug adherence questionnaire was used to score the patients at 6 months of follow-up. According to the score, 406 patients were divided into high adherence group, middle adherence group and low adherence group, the correlation between medication adherence and humanistic factors, chronic diseases and course of disease was analyzed. Results: Compared with the low adherence group, the proportion of age (>60 years old), education (<6 years), no spouse, peasant, monthly income (<3 000 yuan), self-funded medical treatment, insomnia history, solitude and negative life events decreased significantly in the middle and high adherence groups. Cadre ratio and the proportion of workers in the middle adherence group increased significantly (P<0.05). Compared with the low adherence group, the course of disease in the middle and high adherence group was significantly shorter (P<0.01). Logistic regression analysis showed that education < 6 years, no spouse, farmers, low income, self-paid medical treatment, solitude, negative life events, course of disease were independently correlated with low adherence of depression patients (P<0.05 or P<0.01). Conclusions Depression patients have low adherence with medication, so targeted health education should be carried out. Especially for patients with a long course of illness, continuous nursing and long-term follow-up should be done well.Mobilizing family support system , caring for and respecting patients can effectively reduce the occurrence of negative life events. It can improve the medication adherence of depressive patients and promote their early recovery.

Objective Using different sterilization method to sterilize pig specific formula milk powder,exploring the sterilization method and conditions that minimize the loss of nutritional components in formula milk powder.Methods Pig-specific formula milk powder was divided into high-pressure sterilization and irradiation sterilization groups.Formula milk powder in the high-pressure group was sterilized using different sterilization conditions and that in the irradiation group was sterilized using different 60 Co γ-radiation doses.The sterility and the nutritional contents of the sterilized formula milk powders were determined according to national standards.Results The sterility tests for both groups of formula milk powder were negative.Compared to control group,the crude protein contents were significantly lower in formula in the high-pressure group sterilized at 121℃for 30 min and in the irradiation liquid group sterilized at 50 kGy(P<0.01).The water,crude protein,and calcium contents were significantly lower(P<0.001)in the irradiation group sterilized at 50 kGy.There was no significant difference in the valine,isoleucine,or leucine content under 50 kGy sterilization conditions in the irradiation sterilized group,but all amino acid contents were decreased in the high-pressure sterilization and irradiation sterilized liquid groups(P<0.001).Analysis of trace elements showed an increased iron content(P<0.001)in formula sterilized at 121℃for 30 min in the high-pressure sterilization group,increased iron and potassium contents(P<0.001)under 25 kGy sterilization conditions in the irradiation sterilization liquid group,and increased magnesium content(P<0.01).The magnesium(P<0.05)and sodium contents(P<0.01)differed significantly in formula treated under 50 kGy sterilization conditions in the irradiation sterilized powder group.VE and VB2 contents were increased in formula sterilized at 121℃for 30 min in the high-pressure sterilization group(P<0.001),the VE content was increased(P<0.05)and the VB2 content was decreased(P<0.001)in formula sterilized under 50 kGy conditions in the irradiation sterilization liquid group,and the VE and VA contents were decreased in formula sterilized at 25 kGy in the irradiation sterilized powder group(P<0.001).Conclusions Sterilization at 121℃for 30 min result ed in the least loss of nutritional components in the high-pressure sterilization group,while irradiation sterilization result ed in the least loss of nutrients at a dose of 50 kGy.Comparing the two sterilization method,irradiation of milk powder at 50 kGy result ed in the least loss of nutrient content.

Objective:To investigate the curative effect of endoscopic cyclophotocoagulation combined with trabeculectomy for primary open angle glaucoma.Methods:From January 2020 to May 2021, 30 eyes of 30 patients with advanced primary open angle glaucoma with cataract who underwent endoscopic cyclophotocoagulation combined with trabeculectomy in the Municipal Hospital Affiliated to Xuzhou Medical University were retrospectively analyzed. The preoperative best corrected visual acuity, preoperative intraocular pressure (IOP) and numbers of anti-glaucoma medication were recorded. IOP, numbers of anti-glaucoma medication at 1 day, 3 months, 6 months and 1 year after operation, postoperative complications, the numbers of absolute success and qualified success were recorded.Results:All cases were followed up for 1 year. The best corrected visual acuity improved by more than one line in 26 eyes at 1 year after operation. Preoperative visual acuity was maintained in 3 eyes visual acuity decreased in 1 eye. The IOP and numbers of anti-glaucoma medication at 1 day, 3 months, 6 months and 1 year after operation were significantly different from those before operation ( P<0.01). At 1 year after operation, 8 patients were qualified successful and 17 patients were absolutely successful. Early hyphema in 30 eyes, transient ocular hypertension in 3 eyes ciliary body detachment in 0 eyes, corneal edema in 9 eyes, and anterior chamber exudation in 8 eyes. Conclusions:In this small sample size of study with follow-up for 12 months, the results showed that endoscopic cyclophotocoagulation combined with trabeculectomy in the treatment of advanced open angle glaucoma has good preliminary efficacy and safety. However, a large sample of cases is still needed to further study its long-term safety and efficacy.

Objective To investigate the rehabilitation needs and technical support for people with disabilities in rural areas. Methods From October, 2017 to February, 2018, 800 persons with disabilities, aged five to 80 years, from 23 villages in five township, Xi'an, Shaanxi, were surveyed with self-designed questionnaire and interview, including the basic situation, disability and training plan, rehabilitation support and skills maintained. Results The persons were mainly aged 50 to 80 years (58.37%), male (65.37%), accepting middle school education or less (46.63%), married (70.63%), living with their family (77.38%), income less than 2000 Yuan (66%), mainly from their family labor (62.62%). Their disabilities were mainly of grade 3 (40.63%), from hemiplegia (30.63%), dependence in living (45.38%), and no systematic rehabilitation program (55.25%). Most of them needed rehabilitation of self-care (40%), accessible guidance (30.63%) and reimbursement from medicare of Rural Cooperative Medical Scheme (50.63%), and hoped to participate social activities (41.87%). The rehabilitation supports were as that: the rehabilitation services were mobile or none (67.58%), the guiders of rehabilitation were few or none (48.88%), knew some or less rehabilitation knowledge (64.25%), the professionals accepted no continue education training (40%).Conclusion It is important to improve the rehabilitation technical support system for rural people with disabilities.

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide. Fat accumulation "sensitizes" the liver to insult and leads to nonalcoholic steatohepatitis (NASH). G protein-coupled receptor 35 (GPR35) is involved in metabolic stresses, but its role in NAFLD is unknown. We report that hepatocyte GPR35 mitigates NASH by regulating hepatic cholesterol homeostasis. Specifically, we found that GPR35 overexpression in hepatocytes protected against high-fat/cholesterol/fructose (HFCF) diet-induced steatohepatitis, whereas loss of GPR35 had the opposite effect. Administration of the GPR35 agonist kynurenic acid (Kyna) suppressed HFCF diet-induced steatohepatitis in mice. Kyna/GPR35 induced expression of StAR-related lipid transfer protein 4 (STARD4) through the ERK1/2 signaling pathway, ultimately resulting in hepatic cholesterol esterification and bile acid synthesis (BAS). The overexpression of STARD4 increased the expression of the BAS rate-limiting enzymes cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and CYP8B1, promoting the conversion of cholesterol to bile acid. The protective effect induced by GPR35 overexpression in hepatocytes disappeared in hepatocyte STARD4-knockdown mice. STARD4 overexpression in hepatocytes reversed the aggravation of HFCF diet-induced steatohepatitis caused by the loss of GPR35 expression in hepatocytes in mice. Our findings indicate that the GPR35-STARD4 axis is a promising therapeutic target for NAFLD.