OBJECTIVE:To understand the situation of drugs used in this hospital.METHODS:To collect the amount and sum of money of drugs consumed in this hospital in the first halves of 1999,2000 and 2001 and to analyse the frequency of antibiotics used.RESULTS&CONCLUSION:The consumption of antiinfectives,herbal preparations and other commonly-used drugs was in the ratio 2∶4∶4.The use of antibiotics was on the whole rational.

Different types of neurons are regularly dispersed on re tina. For finding the mechanism of eye and retina formation, it is important to know how t his regular pattern is formed during embryonic stage. It was claimed that multip le genes regulate the development of eye and retina. These genes regulate the de velopment of tissues and differentiation of cells in different parts of the visu al system during embryo development. Also, it was found that different tissues a nd differentiated cells cohere with each other both temporally and spatially to form the eye.

Objective To discuss the activity of Toxoplasma gondii and its impact on fetus during pregnancy.Methods Sixty -eight pregnant women whose IgG was positive and IgM was negative for anti - Toxoplasma gondii antibodies were chosen.Their serum and umbilical blood were tested for IgG,IgM,CAg (by ELISA) and DNA (by PCR) of Toxoplasma gondii.The infections of placental samples were tested by the direct smear,homogenate smear and PCR.Results There were 28 positive cases of Toxoplasma gondii IgG antibodies in cord blood,and the vertical transmission rate of IgG in placenta was 41.2%.There were 6 positive cases of Toxoplasma gondii DNA in cord blood,and the incidence of intrauterine infection was 8.8%.There were 9 positive cases of Toxoplasma gondii DNA in placental tissues,and the incidence of intrauterine infection was 13.2%.Conclusions Past Toxoplasma gondii infection during pregnancy may lead to vertical transmission between the mother and infant.

Objective To investigate the concentration of procalcitonin,C-reactive protein and WBC count for patients with dif-ferent kind of bacterial infection.Methods 49 blood samples and 81 other samples were collected form different patients for bacteri-al culture.Meanwhile,procalcitonin,C-reactive protein concentration and WBC count of those patients were determined to find whether these indicators exhibited any difference between Gram-positive and Gram-negative bacteria infected patients.Results For bloodstream infection,PCT levels in patients with Gram-nagetive bacterial infection were obviously higher than those with Gram-positive infection(P <0.05 ).For other infection,there was no statistical difference among patients′PCT,CRP concentration and WBC count(P >0.05).Conclusion PCT concentration is valuable in the differential diagnosis of blood stream infection caused by Gram-negative and Gram-positive bacteria,but not in local infection infection.

Nuclear factor kappa B(NF-?B) is an important transcription factor in the expression of many inflammatory media. With the recent progress of molecular biological technique, the modulated mechanism of NF-?B in immunity, stress, inflammatory responses, and the differentiation and growth of lymphocyte has been the focus. This review presents the role of NF-?B in ischemia-reperfusion and cardiopulmonary bypass, and the research progression of intervention therapy in inhibition of the activation of NF-?B is also introduced herein after.

Physical activity is closely related to the quality of life of postoperative patients with breast cancer, which is expressed in fre-quency, duration, intensity, and others factors. There is no uniform measurement of physical activity, the common measurements may be classified as subjective and objective, such as exercise log, physical activity questionnaire and self-reported physical activities of the former;doubly labeled water, indirect calorimetry, motion sensors and heart rate monitors of the latter. The age, educational level, social and eco-nomic status, and body mass index are related to the level of physical activity in patients post operation for breast cancer, while illness and treatment, psychological and social support, and health-related quality of life also affect the level of physical activity, which suggest that such patients need to improve their physical activity before operation.

Objective To study the mechanism of Qingre Huashi Yiqi Huoxue Formulae (Formulae for clearing heat,dissolving damp,reinforcing qi and activating blood) for H.pylori associated gastric diseases. Methods The 70 C57BL/6 mice were randomly divided into 7 groups with 10 in each:normal group,model group,Chinese medicine high,medium and low dosage groups,western medicine group,and the combination of Chinese and western medicine group. Ampicillin combined with H.pylori gavage was adopted to produce H.pylori infected models. Four weeks later,all the groups were given correspondent treatment. The morphological and pathological changes of the gastric mucosa and the colonization of H.pylori in the gastric mucosa were observed after 2 weeks. ResultsThe Chinese medicine high and medium dosage groups and the combination of Chinese and western medicine group had a better effect in clearing H.pylori (P0.05). The Chinese medicine high and medium dosage groups,western medicine group,and the combination of Chinese and western medicine group had a significant morphological changes (P

The sequence of Neurospora crassa(XP_322380)and Gibberella zeae PH-1(EAA76971)ribosomal protein gene were subjected to local tBlastn searching against the Chaetomium globosum ESTs datebase.The 765 bp full length cDNA encoding 60S ribosomal protein L10a gene was obtained.The open reading frame was 654 bp and encoded 217 amino acids.The protein molecular mass was 23.9 kD.The BlastP analysis revealed that amino acids sequence of ribosomal protein L10a gene from C.globosum shared 89% high similarity with N.crassa and 78% low similarty with Ustilago maydis.The cDNA and deduced amino acid sequence of 60S ribosomal protein L10a gene were accepted by GenBank(accession numbers: AY669070,AAT74578).

Objective To investigate the role of icaritin in delaying the progression of liver cirrhotic process in rats and the related mechanism. Methods For invitro study, primary rat hepatocytes were obtained by perfusing the liver of male Wistar rats; the cultured cells we reexposed to the fresh medium containing CCl4, and then treated with various concentrations of icaritin. The activities of alanine transaninase(ALT) and glutamic-oxaloacetic transaminase(AST) inculture medium were measured with an automatic biochemical analyzer. The intracellular contents of malondialdehyde (MDA) and superoxide dismutase(SOD) were determined by spectro-photometry; the apoptotic cells were detected by the TUNEL method. Forin vivostudy, CCl4-induced experimental rat hepatic fibrosis model was established and was treated with icaritin. Serum levels of ALT, AST, albumin(ALB), andglobulin(GLB) were measured; the pathological changes and collagen-expression in livers were observed by HE staining and immunohistochemistry, respectively. Results CCl4 significantly increased the activities of ALT, AST, and the contents of MDA in culture media of hepatocytes, and significantly decreased the SOD activity. More apoptotic cells were observed in CCl4 group than that in icaritin group. The ALT, AST activities in culture supernatant and the intracellular MDA contents in icaritin group were significantly lower than those in both model group and drug carrier group, while intracellular SOD activity was much higher than that in other two groups(P<0.05). Icaritin also reduced the apoptotic ratios of hepatocytes induced by CCl4 compared with model group(P<0.05 or 0.01). In the in vivo experiment, icaritin treatment significantly reduced serum levels of ALT and AST compared with model group(P<0.05) and greatly improved CCl4-induced liver histopathological injuries and collagen I deposition in the liver tissues. Conclusion Icaritin treatment can attenuate CCl4-induced cirrhosis in rats, atleast in part, by protecting the hepatocytes from peroxidation product.

Objective To explore the effect of Renzhu Jianwei Granules(Granule of Semen Coicis and Rhizoma Atractylodis Macro- cephalae for strengthening the stomach)on the patients with precancerous lesions of gastric cancer(PLGC)and its mechanism. Methods The 58 patients of chronic atrophic gastritis(CAG)complicated with intestinal metaplasia(IM)and/or dysplasia(Dys) were randomized into treatment group and control group with 29 in each.The treatment group was administered Renzhu Jianwei Gran- ules while the control group was given Weifuchun Tablets.The numbers of positive cells of carcinoembryonic antigen(CEA)and cy- clooxygenase 2(COX-2)of gastric mueosa before and after treatment were observed.Results The scores of positive cells of CEA and COX-2 of gastric mucosa of both groups were greatly reduced after treatment(P0.05).Conclusion Renzhu Jianwei Granules can reduce CEA and COX-2 of PLGC patients,possibly being one of the mechanisms in treating PLGC.