1.Clinical comparative observation of SMlLE operation design of different thickness of corneal cap
International Eye Science 2015;(7):1296-1298
AlM: To compare the impact of different thickness of corneal cap design on small incision lenticule extraction ( SMlLE) operation.METHODS: Forty-six cases of myopia patients ( 92 eyes) intends to SMlLE operation in our hospital were collected , and were randomly divided into 2 groups:corneal cap thickness design for 110μm in group A and 120μm in group B. Other operation parameters designs were consistent. All patients were surgeried by the same surgeon. The incidence of opaque bubble layer ( OBL ) , the ratio of difficult separation of lens, uncorrected visual acuity ( UCVA ) of each time points, and spherical equivalent ( SE) were compared.RESULTS: lntraoperative OBL incidence rate of 110μm group was higher than that of 120μm group with significant difference between the two group (P<0. 05). the ratio of difficult separation of lens was significantly different between the two groups: 110μm group was higher than in 120μm group (P<0. 05). The UCVA in the 120μm group was better than that in the 110μm group at postoperative 1 and 7d. But with the passage of time, postoperative 1, 3, and 6mo of UCVA of 2 groups was similar, showed no significant difference (P>0. 05). SE were compared at 7d and 6mo after operation, showed no significant difference (P>0. 05)CONCLUSlON: Compared with 120μm group, corneal cap design SMlLE operation in 110μm group are more prone to OBL and difficult separation of lens, thus affects UCVA and postoperative recovery rate. There is no significant difference in long-term UCVA.
2.Plasma aldosterone/renin ratio——a sensitive screening test and parameter for primary aldosteronism
Mu-Chao WU ; Hua CHENG ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Plasma aldosterene/renin ratio (ARR) is a sensitive screening test and parameter for primary aldosteronism(PA).The use of ARR leads to a marked increase in the detection rate of PA in the hypertensive population.However,ARR remains a nonstandardized test,and the cutoff value of ARR used in the different studies is varied.Further and systematical studies are needed to improve the accuracy of the test.
4.APOPTOSIS OF HUMAN OVARY GRANULOSA CELLS INDUCED BY THE METABOLITES ACYL-COA OF SATURATED FREE FATTY ACIDS
Yiming MU ; Hua ZHENG ; Baoa WANG
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
To investigate the apoptotic effect of free fatty acids (FFAs) on human ovary granulosa cells, the granulosa cells were treated with FFAs in various concentrations for three days, and then the cell viability was determined by trypan blue exclusion method. DNA fragmentation was examined by DNA ladder formation and Annexin V GFP/PI staining of the cells. We also determined if the apoptotic effect of saturated FFAs was mediated by their acly CoA metabolites and measured the expression of apoptosis related genes, Bcl 2 and Bax by Western blot. The results indicated that saturated FFAs induced apoptosis of granulosa cell by their acly CoA metabolites in a dose dependent manner, and led to the down regulation of Bcl 2 and the up regulation of Bax. It is concluded that saturated FFAs can induce apoptosis of human ovary granulosa cells, suggesting that the sexual disorder of obese and insulin resistant women is related to it.
5. Expression of CTGF gene in human thyroid tissues and its clinical significance
Tumor 2008;28(6):506-509
Objective: To investigate connective tissue growth factor (CTGF) mRNA and protein expression levels in thyroid tissues and analyze their relation to clinical and pathological features of thyroid cancer. Methods: This study quantified CTGF mRNA and protein expression levels in 72 pairs of thyroid papillary carcinoma, 45 pairs of thyroid adenoma and 71 pairs of simple goiter and their matched thyroid tissues using real-time fluorogentic quantitative PCR (RFQ-PCR) and immunohistochemistry staining and analyze their relationship with clinicopathological parameters. Results: RFQ-PCR showed that the CTGF mRNA expression was significantly higher in 59.7% (43 of 72) thyroid papillary carcinoma tissues than that in the matched thyroid tissues; CTGF mRNA expression was significantly lower in 66.7% (30/45) thyroid adenoma and 88.7% (63/71) simple goiter tissues than that in their matched normal thyroid tissues, respectively. The result of immunohistochemical staining was consistent with that of RFQ-PCR. Logistic regression showed that female, lymph node metastasis, higher pathological grade, bigger tumor volume and elevated TSH levels were risk factors of higher expression of CTGF mRNA in thyroid papillary carcinoma; female, higher pathological grade and higher TSH level were risk factors of higher expression of CTGF protein in thyroid papillary carcinoma; both bigger tumor volume and lower FT4 level were risk factors of higher expression of CTGF mRNA and protein in thyroid adenoma. Both male, lower FT4 and TSH level were risk factors of higher expression of CTGF mRNA and protein in simple goiter. Conclusion: Over-expression of CTGF was important in the tumorigenesis and progression of thyroid cancer, and the CTGF protein might serve as a valuable target for early diagnosis and treatment of thyroid cancer in clinic.
6.Correlation of tumor necrosis factor-alpha and interleukin-10 expression to acute rejection in complex tissue and serum of rats after laryngeal transplantation
Mu YUAN ; Gang CHEN ; Hua ZHAO ; Jianjun JING
Chinese Journal of Tissue Engineering Research 2011;15(31):5701-5706
BACKGROUND: It remains unclear whether proinflammatory factor, such as tumor necrosis factor-α (TNF-α), γ-interferon and anti-inflammatory cytokine, such as interleukin-10 (IL-10), interleukin-4 (IL-4) levels can change after laryngeal transplantation. OBJECTIVE: To explore the expression of TNF-α and IL-10 in different expressive tissue layers and its relationship during the acute rejection episodes, and to evaluate the role of TNF-α and IL-10 levels in serum for prediction of early acute rejection after laryngeal transplantation. METHODS: Laryngeal heterotopic transplantations were performed in Wistar and Sprague-Dawley rats. According to different dosages of immunodepressant, all recipients were divided into three groups: Group 0 mg, Group 5 mg, and Group 10 mg. Untreated Sprague-Dawley rats served as normal control group. RESULTS AND CONCLUSION: Changes in serum TNF-α and IL-10 levels at postoperation days 3, 7 and 11 were positively correlated with these expressions in the epithelium mucosa and submucosa at various time points after transplantation. These indicate that the high-antigenicity of graft mainly concentrates on the layers of mucosa and submucosa. TNF-α and IL-10 concentrations can serve as indexes for predicting acute rejection after laryngeal transplantation.
7.Comparison and evaluation of the determination of serum CEA between high-throughput ELISA and ECLIA
Xiaofeng MU ; Aifeng ZHOU ; Ziyun ZHAO ; Juan CHEN ; Hua YU
International Journal of Laboratory Medicine 2014;(22):3088-3089
Objective To compare the performance of high‐throughput ELISA and ECLIA in the determination of carcinoembry‐onic antigen (CEA) .Methods The CEA concentration of serum samples were respectively determinate by high‐throughput ELISA and ECLIA ,and the results were compared .Results Two kinds of detection methods could both accurately reflect the concentration of serum CEA .There was no significant difference between the results of two methods (P> 0 .05) .Within the linear range ,the CEA result of high‐throughput was correlate closely with that of ECLIA (r=0 .922 8 ,P<0 .01) .Conclusion High‐throughput ELISA can accurately detect the serum CEA concentration .
8.The effect of behavioral training on neural stem cell differentiation in the dentate gyrus of rats with hippocampal infarction
Yana LI ; Ling LI ; Hua YUAN ; Xiang MU ; Shan JIANG
Chinese Journal of Physical Medicine and Rehabilitation 2009;31(4):219-223
Objective To explore the effect of behavioral training on the differentiation of neural stem cells in the dental gyrus (DG) in rats with hippocampus infarction. Methods Seventy-eight Sprague-Dawley rats were randomized into infarction plus behavior training group, infarction group and control group. Photochemistry method was used to induce hippocampal infarction in rats of the infarction plus behavioral training group and infarc-tion group. At 1 day after surgery, Morris water maze training was used for infarction plus behavioral training group, free-movement without training was performed for infarction group. Double staining immunofluorescence was used to detect the co-expression of bromodeoxyuridine (BrdU) with neuronal nuclei ( NeuN ) or glia fibrillary acidic protein (GFAP) in the DG at different time points. Results Few BrdU/NeuN and BrdU/GFAP double staining cells were observed in the DG of control rats. In the infarction group and infarction plus behavioral training group the number of BrdU/NeuN and BrdU/GFAP double-stained cells increased in the DG on the opposite side compared with the control group on 14th, 21st, 28th and 35th days after surgery (P < 0.05 ). There observed significantly more BrdU/NeuN and BrdU/GFAP double-stained cells in the infarction plus behavioral training group than that in the infarction group on the 14th, 21st, 28th and 35th days after surgery ( P < 0.05 ). Conclusion Behavioral training can accelerate the differentiation of neural stem cells to neuron and astrocyte, by which to promote the re-covery of neural functions.