1.Clinical comparative observation of SMlLE operation design of different thickness of corneal cap
International Eye Science 2015;(7):1296-1298
AlM: To compare the impact of different thickness of corneal cap design on small incision lenticule extraction ( SMlLE) operation.METHODS: Forty-six cases of myopia patients ( 92 eyes) intends to SMlLE operation in our hospital were collected , and were randomly divided into 2 groups:corneal cap thickness design for 110μm in group A and 120μm in group B. Other operation parameters designs were consistent. All patients were surgeried by the same surgeon. The incidence of opaque bubble layer ( OBL ) , the ratio of difficult separation of lens, uncorrected visual acuity ( UCVA ) of each time points, and spherical equivalent ( SE) were compared.RESULTS: lntraoperative OBL incidence rate of 110μm group was higher than that of 120μm group with significant difference between the two group (P<0. 05). the ratio of difficult separation of lens was significantly different between the two groups: 110μm group was higher than in 120μm group (P<0. 05). The UCVA in the 120μm group was better than that in the 110μm group at postoperative 1 and 7d. But with the passage of time, postoperative 1, 3, and 6mo of UCVA of 2 groups was similar, showed no significant difference (P>0. 05). SE were compared at 7d and 6mo after operation, showed no significant difference (P>0. 05)CONCLUSlON: Compared with 120μm group, corneal cap design SMlLE operation in 110μm group are more prone to OBL and difficult separation of lens, thus affects UCVA and postoperative recovery rate. There is no significant difference in long-term UCVA.
2.Plasma aldosterone/renin ratio——a sensitive screening test and parameter for primary aldosteronism
Mu-Chao WU ; Hua CHENG ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Plasma aldosterene/renin ratio (ARR) is a sensitive screening test and parameter for primary aldosteronism(PA).The use of ARR leads to a marked increase in the detection rate of PA in the hypertensive population.However,ARR remains a nonstandardized test,and the cutoff value of ARR used in the different studies is varied.Further and systematical studies are needed to improve the accuracy of the test.
4.APOPTOSIS OF HUMAN OVARY GRANULOSA CELLS INDUCED BY THE METABOLITES ACYL-COA OF SATURATED FREE FATTY ACIDS
Yiming MU ; Hua ZHENG ; Baoa WANG
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
To investigate the apoptotic effect of free fatty acids (FFAs) on human ovary granulosa cells, the granulosa cells were treated with FFAs in various concentrations for three days, and then the cell viability was determined by trypan blue exclusion method. DNA fragmentation was examined by DNA ladder formation and Annexin V GFP/PI staining of the cells. We also determined if the apoptotic effect of saturated FFAs was mediated by their acly CoA metabolites and measured the expression of apoptosis related genes, Bcl 2 and Bax by Western blot. The results indicated that saturated FFAs induced apoptosis of granulosa cell by their acly CoA metabolites in a dose dependent manner, and led to the down regulation of Bcl 2 and the up regulation of Bax. It is concluded that saturated FFAs can induce apoptosis of human ovary granulosa cells, suggesting that the sexual disorder of obese and insulin resistant women is related to it.
5. Expression of CTGF gene in human thyroid tissues and its clinical significance
Tumor 2008;28(6):506-509
Objective: To investigate connective tissue growth factor (CTGF) mRNA and protein expression levels in thyroid tissues and analyze their relation to clinical and pathological features of thyroid cancer. Methods: This study quantified CTGF mRNA and protein expression levels in 72 pairs of thyroid papillary carcinoma, 45 pairs of thyroid adenoma and 71 pairs of simple goiter and their matched thyroid tissues using real-time fluorogentic quantitative PCR (RFQ-PCR) and immunohistochemistry staining and analyze their relationship with clinicopathological parameters. Results: RFQ-PCR showed that the CTGF mRNA expression was significantly higher in 59.7% (43 of 72) thyroid papillary carcinoma tissues than that in the matched thyroid tissues; CTGF mRNA expression was significantly lower in 66.7% (30/45) thyroid adenoma and 88.7% (63/71) simple goiter tissues than that in their matched normal thyroid tissues, respectively. The result of immunohistochemical staining was consistent with that of RFQ-PCR. Logistic regression showed that female, lymph node metastasis, higher pathological grade, bigger tumor volume and elevated TSH levels were risk factors of higher expression of CTGF mRNA in thyroid papillary carcinoma; female, higher pathological grade and higher TSH level were risk factors of higher expression of CTGF protein in thyroid papillary carcinoma; both bigger tumor volume and lower FT4 level were risk factors of higher expression of CTGF mRNA and protein in thyroid adenoma. Both male, lower FT4 and TSH level were risk factors of higher expression of CTGF mRNA and protein in simple goiter. Conclusion: Over-expression of CTGF was important in the tumorigenesis and progression of thyroid cancer, and the CTGF protein might serve as a valuable target for early diagnosis and treatment of thyroid cancer in clinic.
6.A Strategy for Bioproduction of Rare Sugars: Izumoring
Wan-Meng MU ; Tao ZHANG ; Bo JIANG ; Hua ZHANG ;
China Biotechnology 2006;0(07):-
Rare sugars were defined as monosaccharides and their derivatives that rarely exist in nature. They played an important role in food, health, medicine and etc A strategy for bioproduction of rare sugars, namely Izumoring, was described. By the Izumoring method, all monosaccharides and polyols could be linked, using enzymatic reaction with D-tagatose 3-epimerase, aldose isomerases and polyol dehydrogenases. Izumoring for hexoses, pentoses and tetroses were designed respectively. According to this strategy, the bioproduction routes of various rare sugars, using microbial and enzymatic reactions, could be obtained. In addition, the future research tendency of biotransformation of rare sugars was put forward.
7.Identification of a Bacillus sp. Strain with Fibrinolytic Activity and Primary Study on Fermentation Process
Zhu LIU ; Ying HUA ; Bo JIANG ; Wan-Meng MU ;
Microbiology 2008;0(09):-
An extracellar fibrinolytic strain was isolated from fermented shrimp paste. In addition to general physiological and biochemical properties, the strain was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of the strain had high similarity with AY601723 and AB195282, suggesting that the strain is a subspecies of Bacillus sp. It was named as Bacillus sp. nov. SK006 by CCTCC. The medium composition and fermentation conditions for fibrinolytic enzyme production were also optimized in the research.
8.Study on breast-feeding in hepatitis B virus carrier women
Ruili MU ; Yuyan MA ; Hua LI ; Al ET
Chinese Journal of Perinatal Medicine 1998;0(01):-
Objective To explore the possibility of breast feeding in chronic asymptomatic hepatitis B virus (HBV) carriers after immuno prophylaxis of the infants. Methods The infants with asymptomatic HBV carriers mothers were selected by the obstetric department of Qilu Hospital of Shangdong University, Jinan Maternity and Infant Health Institute of Shangdong from Sept 2001 to Oct 2003 prospectively. Umbilical blood HBV deoxyribonucleic acid (HBV DNA) was detected at birth. All infants received 200 IU HBV specific immunoglobin(HBIG)within 12 hours and on 14 days after birth. The hepatitis B recombinant vaccine was given within 24 hours after birth and at 1 and 6 months of age. The way of feeding was chosen by the mothers as they liked. There were 55 infants in breast feeding group and 36 in bottle feeding group. Infants were then followed up at 7 and 12 months of age and tested for hepatitis B surface antigen(HBsAg), hepatitis B surface antibody (anti HBs), hepatitis B e antigen(HBeAg), hepatitis B e antibody(anti HBe) and hepatitis B core antibody(anti HBc) and HBV DNA. Uninfected infants with negative anti HBs were given repeated dose of vaccinations. Results At 7 and 12 months of age, the positive rates of HBV DNA were 9.09%(5/55)and 9.09%(5/55), anti HBs were 85.45%(47/55)and 90.90%(50/55)in breast feeding group respectively;while the positive rates of HBV DNA were 8.33%(3/36)and 8.33%(3/36), anti HBs were 86.11%(31/36)and 91.67%(33/36)in bottle feeding group respectively. No significant differences was shown in positive rates of HBV DNA and anti HBs between these two groups. Conclusions With appropriate immunoprophylaxis, including hepatitis B immune globulin and hepatitis B vaccine, HBV carriers can breast feed their babies.
9.Antigenic analysis of coxsackievirus type A 24 by monoclonal antibodies
Guifan MU ; Hua LV ; Ruliang XU ; Fangzhou GU
Chinese Journal of Immunology 1985;0(03):-
Antigenic analysis of 10 strains of Coxsackievirus type A 24 (CA24) was carried out by monoclonal antibodies (McAbs) prepared with a China isolate of Coxsackievirus A 24 variant (CA24v).8 McAbs which were positive in indirect immunofluorescent test reacted to 9 CA 24 v detected. Of them, 4 McAbs could react to both CA 24v and CA 24 prototype (Joseph strain),and the others did not react to CA 24 prototype. 5 McAbs possessing virus-neutralizing activity did not neutralized CA 24 prototype (Joseph strain)and only one of them could neutralize EH 24/70 strain weakly, three of them neutralize 2 isolate from Japan in 1985-86 (J140/85 and J 60/86) and one isolate Fujian of China in 1986. However, all 5 McAbs above could neutralize isolate from Henan and Shanghai of China in 1986 as well as Beijing, Liaoning and Fujian of China in 1988.
10.The effects of reciprocal inhibition on motor function and brain functional network connectivity of stroke patients
Qiang DUAN ; Xiang MU ; Hua YUAN ; Weidong LI
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(9):680-683
Objective To investigate the effects of reciprocal inhibition on motor function connectivity in the brains of stroke patients.Methods Thirty patients with stroke were randomly divided into a treatment group (n =15) and a control group (n =15).The control group underwent normal limb positioning,medium frequency electrotherapy,circulated compression of the limbs,etc.The treatment group received conventional rehabilitation treatment plus reciprocal inhibition treatment for 30 min daily,6 times a week for 4 weeks.All of the patients were assessed before and after treatment using the Canadian neurological scale (CNS),the Frenchay activities index (FAI),the motricity index (MI) and functional magnetic resonance imaging of the motor cortex in a resting state (rs-fMRI).Results In both groups the average CNS,FAI and MI scores improved significantly.Compared with the control group,the changes in FAI and MI scores in the treatment group improved significantly more.The coefficient of functional connectivity of the bilateral motor cortex decreased significantly after treatment in both groups.In the treatment group the motor cortex functional connectivity correlated significantly with the improvements in MI scores.Conclusions Reciprocal inhibition can accelerate the improvement of extremity motor function and ability in the activities of daily living significantly after stroke.It reduces functional connectivity in the bilateral motor cortex in ways significantly correlated with improvements in motor function.