Aspirin ; administration & dosage ; therapeutic use ; Blood Cell Count ; Blood Platelets ; drug effects ; physiology ; C-Reactive Protein ; analysis ; Child, Preschool ; Diagnosis, Differential ; Female ; Humans ; Hydroxyurea ; administration & dosage ; therapeutic use ; Interferons ; administration & dosage ; therapeutic use ; Platelet Count ; Thrombocythemia, Essential ; blood ; diagnosis ; therapy

Thunderclap headache is a sudden severe headache. It is an uncommon type of headache. Identifying it in time and accurate diagnosis are very important because this type of headache is often complicating serious cerebral diseases. This article reviews its causes and diagnostic assessments.

Objective To investigate the role of Akt signal pathway on the expression of monocyte chemoattractant protein-1 ( MCP-1 ) and nuclear transcription factor-κB (NF-κB) in renal tubular epithelial cells (HK-2) stimulated by albumin and to explore the mechanisms of action. Method The HK-2 cells were incubated in the presence of albumin (5,15,30 mg/mL) with or without Ly294002 (an inhibitor of Akt). Expression of mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR).Expression of Akt and protein MCP-1 were assessed by Western blot. Electrophoretic mobility shift assay (EMSA) was used to detect the activation of NF-κB. q-test was used to evaluate the differences in means between groups. Results Compared with control group, the expression of MCP-1 mRNA remarkly increased. [Control group: 0.233 ±0.01; BSA(5 mg/mL) group: 0.285 ±0.04; BSA( 15 mg/mL) group:0.387 ± 0.02; BSA ( 30 mg/mL) group: 0.473 ± 0.05; BSA ( 30 mg/mL) + Ly294002 group: 0. 325 ±0.05, P ＜ 0.05 ]. The expression of MCP-1 protein in renal interstitum of operation group were remarkly increased too. [ Control group: 100 ± 15.1; BSA ( 5 mg/mL) group: 148 ± 19.3; BSA ( 15 mg/mL) group: 176±20.7; BSA(30 mg/mL) group: 263 ± 18.1; BSA(30 mg/mL) + Ly294002 group: 175 ± 18.0, P ＜0.05 ]. Albumin stimulated the expression of MCP-1mRNA and protein in a dose-dependent manner. Albumin remarkably increased the activity of NF-κB. Albumin enhanced the expression of Akt. Ly294002 inhibited albumin-induced the expression of NF-κB and partially decreased the level of MCP-1. Apositive correlation was noted between NF-κB activation and MCP-1 expression( r = 0.68 ,P ＜ 0.01 ). Conclusions Albumin-induces MCP-1 and NF-κB production via Akt signal pathway in renal tubular epithelial cells.

Objective To observe the effect of atomized inhalation on the levels of serum sICAM-1, SP-A and IGF-1 in children with mycoplasma pneumonia.Methods 108 children with mycoplasma pneumonia in our hospital from January 2015 to September 2016 were selected as the study object, and they were randomly divided into control group and observation group with 54 cases in each group, the control group were treated with conventional treatment, the observation group were treated with pulmicort respules by aerosol inhalation on the basic treatment of control group , then the clinical effective rates and signs disappearing time of different severity degree, serum sICAM-1, SP-A and IGF-1 levels before and after the treatment of two groups were compared.Results The clinical effective rates of observation group with mild, moderate and severe disease were respectively 100.00%, 100.00% and 93.33%, and they were all higher than 84.21%, 80.00% and 73.33% of control group, and the cough, fever and pulmonary rales disappearing time were respectively (4.54 ±0.66)d, (1.84 ±0.18)d and (3.76 ±0.52)d, and they were all shorter than (7.10 ±0.82)d, (3.25 ± 0.30)d and (5.88 ±0.75)d of control group, the serum sICAM-1and SP-A at first, third and fifth day after the treatment were lower than those of control group, serum IGF-1 level were higher than those of control group, the difference was statistically significant (P<0.05), the differences were all significant.Conclusion The clinical effect of pulmicort respules by aerosol inhalation is better, and it has active adjustion role for the serum sICAM-1, SP-A and IGF-1.

Objective To observe the expression of polysialic acid-neural cell adhesion molecule (PSA-NCAM) in the rat hippocampus after infrasound exposure. Methods Ninety-six Sprague-Dawley rats were random-ized into a 16 Hz 130 dB infrasound exposure group (n =72) and a control group (n =24). Rats of the infrasound exposure group were exposed to 16 Hz 130 dB infrasound in a barochamber 2 h/d for 1, 7, 14 and 21 d. The rats' brains were removed and immunohistochemieal methods were used to detect the expression of PSA-NCAM in the hip-pocampus on the 1st, 7th, 14th, and 21st day during treatment and on the lat, 7th, and 14th day after the treatment ended. Results The expression of PSA-NCAM in the rats' hippocampuses increased after exposure for 1 d, reached its peak at the 14th day, then had decreased by the 21st day but remained at a higher level than in the controls (P≤ 0.05). Conclusion The expression of PSA-NCAM in the rat hippocampus is increased by exposure to 16 Hz 130 dB infrasound, but the levels recover somewhat after infrasound exposure ends. lnfrasound could induce neural injury and promote the migration of neural stem cells. PSA-NCAM might participate in the repair of neural injuries re-suiting from infrasound exposure.

Objective To study the effect of 16 Hz infrasound on the proliferation of the neural stem cells in rats' hippocampus.Methods72 SD rats were randomized into 16 Hz-130 dB infrasound exposure group(n=24),16 Hz-90 dB infrasound exposure group(n=24)and control group(n=24).Rats of the infrasound exposure groups were exposed to 16Hz 90dB/130dB infrasound barochamber system,2 h/d for 1 d,7 d,14 d,or 21 d.Their brains were removed and anti-nestin immunohistochemistry was used to observe the neural stem cells in rats' hippocampus.ResultsThe expression of nestin in rats' hippocampus increased after exposure for once and increased with the exposure time.It reached its peak on the 14th day,decreased on the 21st day but remained higher level.The reaction of 90 dB group was weaker than that of 130 dB group.ConclusionThe brain injuries result from 16 Hz infrasound exposure can enhance the proliferation of neural stem cells in rats' hippocampus which might participate in the repair of neural injuries.

Animals ; Collagenases ; metabolism ; Dental Bonding ; Dental Caries ; enzymology ; Dentin ; enzymology ; pathology ; Gelatinases ; metabolism ; Humans ; Matrix Metalloproteinase 14 ; metabolism ; Matrix Metalloproteinase 20 ; metabolism ; Matrix Metalloproteinase 3 ; metabolism ; Matrix Metalloproteinases ; metabolism ; Sclerosis

The purpose of this study is to assess the nutritional value and biological effect of Collybia Velutipes. The results were as follows: (1) 10% Collybia Velutipes extract contains 19210mg/L of total amino acid. It is more than the content in the equal amount of mushrooms. (2) The kindergarten children were fed with Collybia Velutipes product for 7 weeks. It was found that the height and the circumference of the mid-upper arm in the children were significantly higher than the control. (3) Weanling male and female rats of wistar strain were divided into two groups ana led the following diets: group l(control), Collybia Velutipes (CV) free diet; group 2(subject), diet containing 25% CV product which contains 2.5% dried CV. The rats were fed the diet ad libitum for 6 weeks. At the end of the experiment, we found that the food effect (FE) of the subject group was significantly higher than the control (P

Objective To study the pathogenesis of rotavirus(RV) diarrhea.Methods Simian rotavirus(SA-11) was grown in cultured MA-104 cell.The viral titers of the culture supernatant were determined by plaque forming assay.KM mice aged 7 days were inoculated with the viral supernatant via feeding tube(gavage).Histological and ultramicrostructure changes of the small intestines were observed under light microscope and electron microscope.The values of crypt depth and villi height were measured with software(image pro plus 5.1,IPP5.1).The distribution of the RV antigen in small intestine and the filamentous actin of the small intestine chorioepithelium were observed with immunohistochemical techniques.The apoptosis of the small intestine epithelium cells was observed with an in situ apoptosis detection kit.Results There were mild hyperemia,dropsy and extensive vacuolar degeneration of small intestine villi under light microscope.Plenty of lipid droplet-like structure at the top of the villi,microvilli malalignment or defluxion and enterocyte defluxion could be seen by electron microscope,but no obvious structure changes at the cell junctions were seen.The RV antigen mainly distributed at the top of the villi.The quantity of small intestine filamentous actin decreased and enterocyte apoptosis increased after RV infection.Conclusion RV mainly infects the mature villous epithelium.The presentation of RV diarrhea relates to the lesion of cytoskeleton,the microvilli lesion of the small intestine,enterocyte apoptosis and defluxion,villi atrophy,etc.,but may have no relationship with the structural changes of cell junctions in the small intestine epithelium.

Knowledge-attitude-practice model is widely used in the self-management of type-2 diabetes.Through proper education,patients can gain diabetic knowledge as well as positive attitude on glucose control.By regulating diet,exercise,self glucose monitoring and medication compliance,diabetic patients are able to control blood glucose more effectively,and have less complications and higher quality of life.Researches on the application and effectiveness of various diabetic knowledge,attitude and self-management behavior scales are reviewed in this article.