Aspirin ; administration & dosage ; therapeutic use ; Blood Cell Count ; Blood Platelets ; drug effects ; physiology ; C-Reactive Protein ; analysis ; Child, Preschool ; Diagnosis, Differential ; Female ; Humans ; Hydroxyurea ; administration & dosage ; therapeutic use ; Interferons ; administration & dosage ; therapeutic use ; Platelet Count ; Thrombocythemia, Essential ; blood ; diagnosis ; therapy

Thunderclap headache is a sudden severe headache. It is an uncommon type of headache. Identifying it in time and accurate diagnosis are very important because this type of headache is often complicating serious cerebral diseases. This article reviews its causes and diagnostic assessments.

Objective To investigate the role of Akt signal pathway on the expression of monocyte chemoattractant protein-1 ( MCP-1 ) and nuclear transcription factor-κB (NF-κB) in renal tubular epithelial cells (HK-2) stimulated by albumin and to explore the mechanisms of action. Method The HK-2 cells were incubated in the presence of albumin (5,15,30 mg/mL) with or without Ly294002 (an inhibitor of Akt). Expression of mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR).Expression of Akt and protein MCP-1 were assessed by Western blot. Electrophoretic mobility shift assay (EMSA) was used to detect the activation of NF-κB. q-test was used to evaluate the differences in means between groups. Results Compared with control group, the expression of MCP-1 mRNA remarkly increased. [Control group: 0.233 ±0.01; BSA(5 mg/mL) group: 0.285 ±0.04; BSA( 15 mg/mL) group:0.387 ± 0.02; BSA ( 30 mg/mL) group: 0.473 ± 0.05; BSA ( 30 mg/mL) + Ly294002 group: 0. 325 ±0.05, P ＜ 0.05 ]. The expression of MCP-1 protein in renal interstitum of operation group were remarkly increased too. [ Control group: 100 ± 15.1; BSA ( 5 mg/mL) group: 148 ± 19.3; BSA ( 15 mg/mL) group: 176±20.7; BSA(30 mg/mL) group: 263 ± 18.1; BSA(30 mg/mL) + Ly294002 group: 175 ± 18.0, P ＜0.05 ]. Albumin stimulated the expression of MCP-1mRNA and protein in a dose-dependent manner. Albumin remarkably increased the activity of NF-κB. Albumin enhanced the expression of Akt. Ly294002 inhibited albumin-induced the expression of NF-κB and partially decreased the level of MCP-1. Apositive correlation was noted between NF-κB activation and MCP-1 expression( r = 0.68 ,P ＜ 0.01 ). Conclusions Albumin-induces MCP-1 and NF-κB production via Akt signal pathway in renal tubular epithelial cells.

Objective To observe the expression of polysialic acid-neural cell adhesion molecule (PSA-NCAM) in the rat hippocampus after infrasound exposure. Methods Ninety-six Sprague-Dawley rats were random-ized into a 16 Hz 130 dB infrasound exposure group (n =72) and a control group (n =24). Rats of the infrasound exposure group were exposed to 16 Hz 130 dB infrasound in a barochamber 2 h/d for 1, 7, 14 and 21 d. The rats' brains were removed and immunohistochemieal methods were used to detect the expression of PSA-NCAM in the hip-pocampus on the 1st, 7th, 14th, and 21st day during treatment and on the lat, 7th, and 14th day after the treatment ended. Results The expression of PSA-NCAM in the rats' hippocampuses increased after exposure for 1 d, reached its peak at the 14th day, then had decreased by the 21st day but remained at a higher level than in the controls (P≤ 0.05). Conclusion The expression of PSA-NCAM in the rat hippocampus is increased by exposure to 16 Hz 130 dB infrasound, but the levels recover somewhat after infrasound exposure ends. lnfrasound could induce neural injury and promote the migration of neural stem cells. PSA-NCAM might participate in the repair of neural injuries re-suiting from infrasound exposure.

Objective To observe the effect of atomized inhalation on the levels of serum sICAM-1, SP-A and IGF-1 in children with mycoplasma pneumonia.Methods 108 children with mycoplasma pneumonia in our hospital from January 2015 to September 2016 were selected as the study object, and they were randomly divided into control group and observation group with 54 cases in each group, the control group were treated with conventional treatment, the observation group were treated with pulmicort respules by aerosol inhalation on the basic treatment of control group , then the clinical effective rates and signs disappearing time of different severity degree, serum sICAM-1, SP-A and IGF-1 levels before and after the treatment of two groups were compared.Results The clinical effective rates of observation group with mild, moderate and severe disease were respectively 100.00%, 100.00% and 93.33%, and they were all higher than 84.21%, 80.00% and 73.33% of control group, and the cough, fever and pulmonary rales disappearing time were respectively (4.54 ±0.66)d, (1.84 ±0.18)d and (3.76 ±0.52)d, and they were all shorter than (7.10 ±0.82)d, (3.25 ± 0.30)d and (5.88 ±0.75)d of control group, the serum sICAM-1and SP-A at first, third and fifth day after the treatment were lower than those of control group, serum IGF-1 level were higher than those of control group, the difference was statistically significant (P<0.05), the differences were all significant.Conclusion The clinical effect of pulmicort respules by aerosol inhalation is better, and it has active adjustion role for the serum sICAM-1, SP-A and IGF-1.

Objective To study the effect of 16 Hz infrasound on the proliferation of the neural stem cells in rats' hippocampus.Methods72 SD rats were randomized into 16 Hz-130 dB infrasound exposure group(n=24),16 Hz-90 dB infrasound exposure group(n=24)and control group(n=24).Rats of the infrasound exposure groups were exposed to 16Hz 90dB/130dB infrasound barochamber system,2 h/d for 1 d,7 d,14 d,or 21 d.Their brains were removed and anti-nestin immunohistochemistry was used to observe the neural stem cells in rats' hippocampus.ResultsThe expression of nestin in rats' hippocampus increased after exposure for once and increased with the exposure time.It reached its peak on the 14th day,decreased on the 21st day but remained higher level.The reaction of 90 dB group was weaker than that of 130 dB group.ConclusionThe brain injuries result from 16 Hz infrasound exposure can enhance the proliferation of neural stem cells in rats' hippocampus which might participate in the repair of neural injuries.

Animals ; Collagenases ; metabolism ; Dental Bonding ; Dental Caries ; enzymology ; Dentin ; enzymology ; pathology ; Gelatinases ; metabolism ; Humans ; Matrix Metalloproteinase 14 ; metabolism ; Matrix Metalloproteinase 20 ; metabolism ; Matrix Metalloproteinase 3 ; metabolism ; Matrix Metalloproteinases ; metabolism ; Sclerosis

OBJECTIVETo observe the clinical effect of Ziyin Huoxue Granule (ZHG) combined glucocorticoids and antibiotics in treatment of radiation pneumonitis.

METHODSTotally 70 radiation pneumonitis patients were assigned to the treatment group and the control group according to random digit table, 35 in each group. All patients received glucocorticoids and antibiotics. Patients in the treatment group additionally took ZHG, one dose per day for 4 successive weeks. Watters clinical-radiologic-physiologic (CRP) score, Karnofsky Performance Status Scale (KPS) , and acute radiation injury classification [set by Radiation Therapy Oncology Group (RTOG)] were observed in the two groups before and after treatment. The application time for antibiotics and glucocorticoids was compared between the two groups.

RESULTSAll patients completed this trial, and nobody dropped out or died. There was no statistical difference in Watters-CRP scores, KPS, or RTOG between the two groups before treatment (P > 0.05). Compared with before treatment in the same group, RTOG classification was obviously improved in the two groups (P < 0.05). Compared with the control group, Watters-CRP scores decreased, KPS increased, the application time for antibiotics and glucocorticoids was reduced (P < 0.05). The efficacy of RTOG classification was better in the treatment group than in the control group, but with no statistical difference between the two groups (P > 0.05).

CONCLUSIONZHG combined glucocorticoids and antibiotics was superior in treating radiation pneumonitis to using glucocorticoids or antibiotics alone in elevating Watters-CRP scores, shortening the application time for glucocorticoids and antibiotics, and improving patients' physical conditions.

Anti-Bacterial Agents ; therapeutic use ; Drug Therapy, Combination ; methods ; Drugs, Chinese Herbal ; therapeutic use ; Glucocorticoids ; therapeutic use ; Humans ; Karnofsky Performance Status ; Radiation Pneumonitis ; drug therapy

Objective To investigate dry chemical analysis of urine,automated quantitative anal-ysis of urine formed elements and urine living cells staining microscope extination combination of the three urine red blood cells for a variety of detection methods in the comprehensive analysis of renal dis-ease in the clinical application. Methods Gemany Miditron Junior Ⅱ of urine analyzer for chemical a-nalysis of urine. UF-1 00 automatic urine visible component analysis(referred to: UF-1 00)living cells (SM)staining, The difference in the imaging system under the microscope, in the urine of red blood cells to identify patterns observed. Results Urine dry chemical analysis,automated quantitative analy-sis of urine fomed elements and ,urlne staineg cells microscope examination of the three organic combi-nation of a variety of detection methods for urine analysis, Application of this paper, Detection of a va-riety of red blood cells urine analysis-urine flow chart of sources of identification laboratory, Improve the analysis of the urine test quality, efficiency and laboratory dinosis, made up of these expenmental methods of the deficiencies. Conclusion Kidney disease is extremely valuable to provide obj ective indi-cators, is in clinical methods.

Objective To assess the influencing factors of the progression of carotid atherosclerotic stenosis using color Doppler flow imaging (CDFI). Methods From January 2009 to December 2014, a total of data 279 consecutive patients first assessed by CDFI as moderate stenosis of carotid atherosclerosis (stenosis rate 50 -69%)and regularly reexamined with CDFI at 12,24 and 36 months after initial examination were enrolled retrospectively. The residual diameter of vascular lesions and the changes of hemodynamic parameters were documented,and they were divided into either a progression group (n = 40)or a non-progression group (n = 239,and the non-progression group was divided into steady group[n = 210]and improved group [n = 29])according to whether the degree of stenosis progressed into severe stenosis (stenosis rate 70 -99%)or occlusion. The effects of the risk factors for common cerebrovascular disease and taking lipid lowering drugs (atorvastatin 20 mg/ d)on stenosis progression were compared in patients between the 2 groups. There were significant differences in hypertension,smoking and the regular use of atorvastatin . The effects of those factors on the progression of carotid stenosis were compared further through Logistic regression analysis. Results The residual vascular diameters of stenosis at 24,and 36 months were reduced obviously in the progression group compared with those of the non-progression group. There was significant difference (all P < 0. 05),and both the stenotic sites and distal peak systolic flow velocity ratio were significantly higher than those of the steady group and improved group (all P < 0. 05). Among the risk factors for cerebrovascular disease,hypertension (OR,2. 686,95% CI 1. 120 -6. 442,P = 0. 027)and smoking (OR,2. 265,95% CI 1. 081 -4. 746,P = 0. 030)were the major risk factors for affecting the progression of carotid stenosis. Regularly taking atorvastatin was a protective factor of delaying the progression of carotid stenosis (OR,0. 383,95% CI 0. 178 -0. 827,P = 0. 015). Conclusions CDFI may objectively evaluate the progression of carotid stenosis. Smoking and hypertension are the independent risk factors for affecting the progression of carotid stenosis,and regularly taking atorvastatin contributes to delay the progression of carotid stenosis.