OBJECTIVE:A RP-HPLC method was developed for determination of cefetamet in serum METHODS:The serum samples were detected on a hypersil BDS C18 column after preciptating protein with methyl alcohol and being centrifuged at a speed of 10 000 r/min The mobile phase was methyl alcohol-water=28∶72(V/V),pH=2 8,the flow rate was 1 0ml/min and the detection wavelength 262nm RESULTS:The methods showed good linearity in a concentration range of 0 53～13 25?g/ml The minimun detactable concentration of cefetamet in serum was 0 25?g/ml,the mean recovery was 100 84%,the within-day RSD was less than 4 42% and the between-day RSD was less than 4 17% CONCLUSION:The method is simple,sensitive and accurate,and may be applied to the pharmacokinetics study of cefetamet in vivo

octreotide、Ranitidlne、Cimetidine、Nizatidine、Famotidine　Amikacin、Gentamiein、Tobramycin、PeniciUins and Vancomycin added into total parenteral nutritions, respeclwel. The methods we used was to inspect visually, measure the pH, particle size distribution and concentrations after storage for various duration in different temperatures. The results some drugs were stable in total parenteral nutritions,and the others are not.

ObjectiveTo investigate the clinical value of routine para - aortic lymphadenectomy in patients with stage Ⅰ B1 and Ⅱ A2 cervical cancers treated by surgical intent.Methods240 patients with stage Ⅰ B1 and Ⅱ A2 squamous cervical cancer were randomly divided into tow groups( group A and B).120 patients (group A) underwent radical hysterectomy with systematic pelvic lymphadenectomy and paraaortic lymphadenectomy.Other 120 patients (group B) underwent radical hysterectomy with systematic pelvic lymphadenectomy,additional para-aortic lymphadenectomy were carried out when para-aortic lymph nodes were identified as suspicious by visualization and palpation.The operation time,amount of surgical bleeding,length of stay,complications,and metastasis rates of pelvic lymph node,metastasis rates of common iliac lymph node and metastasis rates of para-aortic lymph node were analyzed between two groups.ResultsThe clinical characteristics of the patients had no statistically significant difference between the two groups.The length of stay and the complications of group A were significantly higher than group B ( P ＜0.05 ).During the 240 patients,the metastasis rates of pelvic lymph node,common iliac lymph node and para-aortic lymph node were 27.9％,10.8％ and 5.4％.The metastasis rate of para-aortic lymph node in group A was statistically higher than that in group B( 8.3％ vs 2.5％,P ＜0.05),whereas there had no significant difference of the metastasis rates of pelvic lymph node and common iliac lymph node between the two groups.ConclusionsRoutine para-aortic lymphadenectomy can be helpful to accurately predict the status of the para-aortic lymph node in patients with stage Ⅰ B1 and Ⅱ A2 cervical cancer,but the length of stay and the complications of operation will increase.

The imaging appearance of progressive multifocal leukoencephalopathy (PML) in acquired immunodeficiency syndrome (AIDS), including CT and MRI appearance, the differential diagnosis between PML and other similarly diseases were reviewed.

Physical activity is closely related to the quality of life of postoperative patients with breast cancer, which is expressed in fre-quency, duration, intensity, and others factors. There is no uniform measurement of physical activity, the common measurements may be classified as subjective and objective, such as exercise log, physical activity questionnaire and self-reported physical activities of the former;doubly labeled water, indirect calorimetry, motion sensors and heart rate monitors of the latter. The age, educational level, social and eco-nomic status, and body mass index are related to the level of physical activity in patients post operation for breast cancer, while illness and treatment, psychological and social support, and health-related quality of life also affect the level of physical activity, which suggest that such patients need to improve their physical activity before operation.

Mesenchymal stem cells is another adult stem cells in bone marrow apart from hematopoietic stem cells.Because of their strong self-renewal capacity and the potential of multi-lineage differentiation.mesenehymal stem cells are used more and more in the treatment of malignant diseases,such as hematological malignant diseases and extensively applied in regenerative medicine and tissue engineering.However,the exact role of mesenehymal stem cells on tumor is not completely clear.This review is about the impact of mesenchymal stem cells on tumor growth and the related mechanism.

OBJECTIVE:To reference clinical utilization of amikacin of a hospital.METHODS:By retrospective survey,the clinical information of 30 inpatients who were treated with amikacin and received plasma concentration monitoring from Apr.2006 to Feb.2010 were analyzed statistically in respect of primary disease,results of pathogen culture and drug sensitivity test,dosages and usage,plasma concentration,clinical efficacy,adverse drug reactions,etc.RESULTS:Clinical response rate was 55.2% and dosage of amikacin was small as well as measured value and reference value of plasma concentration.No adverse drug reaction was observed.CONCLUSION:The proportion of amikacin should be increased and plasma concentration monitoring of amikacin should be strengthened.Individual regimen is realized to improve effectiveness and safety of drug use.

OBJECTIVE:To evaluate clinical utilization and blood concentration monitoring of vancomycin in our hospital. METHODS: By retrospective survey method, the clinical information (primary diseases, bacteriological culture, blood concentration of vancomycin, clinical efficacy, renal function) of 41 inpatients who had been treated with vancomycin and their blood concentrations from Nov. 2005 to Dec. 2008 were analyzed statistically. RESULTS: The total response rate of vancomycin was 65.0%. The peak concentration of 62.7% cases were below 25 mg?L-1 while the valley value of 55.2% cases were below 5 mg?L-1. The incidence of renal dysfunction after treatment was 12.2%. CONCLUSION: The consumption and blood concentrations of vancomycin are close to low level in our hospital. It should be paid attention to the blood concentration monitoring of vancomycin to achieve an ideal individual administration according to clinical practice.

Objective:To discuss the effect of AngⅡ on insulin gene expression IN RIN-m cells and its molecular mechanism. Methods:RIN-m cells were cultured and divided into three groups, including the control group, 100 nmol·L-1 AngⅡ group and losartan pretreatment group. After 24-hour incubation, insulin gene expression in RIN-m cells was detected by RT-PCR, the mean flu-orescent intensity of 2', 7'-dichlorofluorescein ( DCF) was detected by flow cytometry, PDX-1 and MafA mRNA expression were detec-ted by RT-PCR and the protein expression was detected by Western-blot. Results: Insulin expression in RIN-m cells, cellular ROS level, PDX-1 expression and MafA expression in 100 nmol · L-1 AngⅡ group were significantly different from those in the control group and losartan pretreatment group (P<0. 05). The later two groups had no significant differentces in those indices (P>0. 05). Conclusion:AngⅡ can down-regulate PDX-1 and MafA expression inβ-cells through oxidative stress pathway, and then inhibit insu-lin gene expression. Pretreatment with losartan can antagonize the effect of AngⅡ, and has protective effect onβ-cells in the aspect of insulin gene expression.

Scholars have focused on the relationship between Henoch-Sch?nlein purpura nephritis and IgA nephropathy in children, when they gradually recognizes the similarities and differences between Henoch-Sch?nlein purpura nephritis and IgA nephropathy in their incentives, genetic factors, renal immunopathology, therapy and so on,with aberrantly glycosylated IgA1 involved in the pathogenesis of Henoch-Sch?nlein purpura nephritis and IgA nephropathy. This paper will review their differences and similarities,along with their relation, according to the research progress.