Objective To investigate the possibility that the construction and expression of a eukaryotic expression vector system of rat NN-NAT gene. Methods The full-length cDNA fragment of rat AA-NAT gene was amplified by RT-PCR method.After retrieving the PCR products,ligating it with pTARGET~(TM) vector,transformating ligation reaction to JM109 huge efficiency competent cells and identifying the recombinant plasmid,the recombinant eukaryotic expression vector pTARGET~(TM)-AANAT was transfected into rat L6 myoblasts with lipofectamine.Accordingly,engineered cells selected by antibiotic G418 were detected by the methods of RT-PCR and Westem blotting. Results It was revealed that,amplified AA-NAT cDNA confirmed by agarose gel electrophoresis could ligate with pTARGET~(TM) vector and subcloned into JM109 cells.L6 cells transfected with pTARGET~(TM)-AA-NAT survived well after G418 selection and expressed AA-NAT protein. Conclusion Our results suggest that we have prepared rat AA-NAT stable eukaryotic expression system successfully although it was just a primary result.This system can be used for the transfection of L6 myoblast.

Cardiomyopathies ; diagnosis ; etiology ; therapy ; Humans

OBJECTIVE:To systemically review the efficacy and safety of Ambroxol hydrochloride injection by intravenous drip combined with aerosol inhalation in the treatment of neonatal pneumonia,and provide evidence-based reference for the clinical treatment. METHODS:Retrieved from PubMed,Medline,CBM,CJFD,VIP and Wanfang Database,randomized controlled trials (RCT)about Ambroxol hydrochloride injection by intravenous drip combined with aerosol inhalation(test group)versus Ambroxol hydrochloride injection alone (control group) in the treatment of neonatal pneumonia. Meta-analysis was performed by using Rev Man 5.2 software after data extracting and quality evaluating by Cochrane 5.1.0. RESULTS:Totally 14 RCTs were enrolled,involv-ing 1 407 patients. Results of Meta-analysis showed the clinical efficacy[RR=1.19,95% CI(1.13,1.24),P<0.001] in test group was significantly higher than control group,the time of cough disappearance[MD=-1.75,95%CI(-1.91,-1.58),P<0.001], asthma disappearance[MD=-1.38,95%CI(-1.67,-1.08),P<0.001] and pulmonary rales disappearance [MD=-1.44,95%CI (-1.77,-1.11),P<0.001] and hospitalization days[MD=-2.04,95%CI(-2.25,-1.82),P<0.001] were significantly shorter than control group,the differences were statistically significant;and there was no significant difference in the incidence of adverse reactions between 2 groups[RR=1.00,95%CI(0.49,2.05),P=1.00]. CONCLUSIONS:Both the efficacy and safety of Ambroxol hydrochloride injection by intravenous drip combined with aerosol inhalation are good in the treatment of neonatal pneumonia.

Batch fermentation of lycopene by recombinant E. c ol i ZYL-2 at various temperatures ranging between 23℃～37℃ were studied in 7L fermentor. At 33℃, cell specific growth rate in earlier stage of culture was higher, and the time obtained maximum cell dry weight was shorter at other tempe rature. while after 9 h, lycopene specific production rate was higher at 28℃. Based on these results, a two-stage temperature control strategy was developed in which 33℃ was used for fermentation for the first 9 h and the temperaturew as switched to 28℃ after 9h. Using this temperature-shift strategy, the maxim al lycopene content and productivity reached 605.25?g L-1 and 28.82?g L-1h-1. The lycopene fermentation level obtained by the strategy was higher than those in single temperature-control experiments.

OBJECTIVE To d e f i n e t h e microsurgical anatomy and exposure obtained by the maxillary swing approach. METHODS Ten adult cadaver specimens fixed by formalin solution were dissected under 5 to 20 times magnification. The microsurgical anatomy and measurement were performed by mimicking the maxillary swing approach. The anatomic relationship of the muscles, arteries,veins,nerves were carefully recorded. RESULTS The maxillary swing approach provides wide exposure extending from the central base to the lateral cranial base,including turkish saddle,the ipsilateral cavernous sinus,the clivus and C1,the nasopharynx,the pterygopalatine and infratemporal fossa,and the parapharyngeal space,etc. CONCLUSION The maxillary swing approach provides a suitable route for accessing lesions involving both the central and lateral cranial base. The sphenoid pterygoid process,the Eustachian tube,and the lateral pharyngeal wall separates the central cranial base from the lateral cranial base and limits anterolateral access to the central cranial base. Removing the pterygoid process provides exposure extending from the central to the lateral cranial base and allows for wide anterolateral access to the clivus and upper cervical spine. The maxillary swing approach is difficult and limited to reach the poststyloid compartment of the parapharyngeal space,the lateral region of styloid process,the horizontal segment of the petrous carotid.

Antihypertensive Agents ; administration & dosage ; therapeutic use ; Humans ; Hypertension ; drug therapy ; genetics ; Molecular Biology

OBJECTIVE: To study the pulmonary function and nasal resistance characteristics of patients with chronic nose-sinusitis and nasal polyps (CRSwNP), to explore the evaluation role of nasal resistance in nasal ventilation function and the effect of endoscopic sinus surgery on pulmonary function in patients with CRSwNP. METHOD: Fifty CRSwNP patients that met the study criteria were selected . The patients were performed endoscopic surgeries according to Messerklinger surgical procedures under general anesthesia. Extent of surgery was based on preoperative CT showing the range of the lesion of disease and endoscopic findings. Perioperative treatments contained intranasal corticosteroids, cephalosporin or penicillin antibiotics, nasal irrigation and other treatments. Main outcome measures included visual analog scale (VAS), endoscopic Lind-Kennedy scores, nasal resistence, pulmonary function in patientsone week before and after surgery, three months and six months after surgery. Pulmonary function includes forced expiratory volume in one second (FEV1), forced vital capacity FEV1/FVC and peak expiratory flow (PEF). RESULT: The study found that there were significantly positive correlations among VAS score, Lund-Kennedy score and nasal resistance (P < 0.05) in CRSwNP patients, but there is a significantly negative correlation between VAS score, Lund-Kennedy score, nasal resistance and pulmonary function indexes of FEV1, FVC and PEF (P < 0.05). The VAS score, Lund-Kennedy score and nasal resistance values of CRSwNP patients were decreased significantly after comprehensive treatments with nasal endoscopic operation as the major one, the difference was statistically different (P < 0.05). And the pulmonary function indexs (FEV1, FVC, PEF) were significantly increased after surgery in CRSwNP patients. CONCLUSION The nasal resistance can objectively and reliably reflect the degree of nasal congestion and the recovery of nasal function in CRSwNP patients after endoscopic sinus surgery. The detection method of nasal resistance is simple. Functional endoscopic sinus surgery can effectively improve the pulmonary ventilation function in CRSwNP patients, providing some clinical references about the prevention and treatment of CRS related lower respiratory disease.
Administration, Intranasal ; Adrenal Cortex Hormones ; therapeutic use ; Anti-Bacterial Agents ; therapeutic use ; Chronic Disease ; Endoscopy ; Humans ; Nasal Lavage ; Nasal Polyps ; diagnosis ; Nose ; physiopathology ; surgery ; Pulmonary Ventilation ; Respiratory Function Tests ; Rhinitis ; Sinusitis ; diagnosis ; surgery

Survivin expresssion in embryo spleen, embryo kidney, embryo liver and in many cancer tissues was determined by RT-PCR, while not in the health liver tissue. Construct the engineered Escherischia coli expressing human survivin and identify the expressed human survivin by Westen-blot. The combination activity of Survivin and RhSmac was determined in vitro. L929 cells transferred with Survivin can survive longer than which transferred with BSA.

Acupuncture Points ; Asthma ; drug therapy ; Bronchitis ; drug therapy ; Child, Preschool ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Infant ; Male

Objective　To quantify the characteristics of the auditory brainstem responses(ABR) in the normal newborns, in order to provide a data for newborn hearing screening. Methods　The ABR to click stimuli was investigated in a group of eighty normal newborns aged from 3 to 8 days and compared to those observed from the normal hearing youths. Results　Wave, Ⅰ,Ⅲ and Ⅴ were identifiable in all of 160 ears at 130 dB pe-SPL. The threshold of wave Ⅴ to click stimuli was 61.94±6.87 dB peSPL, significantly higher than that in adults. Both latencies for wave Ⅰ,Ⅲ and Ⅴ and interwaves latencies Ⅰ-Ⅲ,Ⅲ-Ⅴ and Ⅰ-Ⅴ were significantly tonger than those for the adults under identical test conditions. The ratio of interwaves latency Ⅲ-Ⅴ to Ⅰ-Ⅲ for normal newborn was 0.71±0.12, statisitically smaller than that in adults. Conclusion　Click level 70 dB peSPL appeared to be a feasible criterion of threshold of ABR for newborn hearing screening. There were no significant effects of sex and ear in newborn hearing screening. The auditory system of the newborns was not equally mature. The neurological system is the primary source of differences between newborns and adults.