1. The role of diffusion-weighted MR imaging in diagnosis of bladder cancer
Tumor 2012;32(12):1025-1029
Objective: To evaluate the value of DWI (diffusion-weighted imaging) of MR (magnetic resonance) and ADC (apparent diffusion coefficient) in diagnosis, staging and grading of bladder cancer. Methods: The DWI images of 73 patients with bladder masses were collected and the diagnostic sensitivity, specificity and accuracy of bladder cancer were evaluated. The ADC values of bladder cancer, the surrounding structures, the urine and the bladder inflammation were measured. The staging based on the DWI of bladder cancer was compared with pathology. The ADC values of different grades of urothelial carcinoma were determined and compared. Results: The diagnostic sensitivity, specificity and accuracy of bladder cancer by DWI were 92.3%, 75.0% and 90.4%, respectively. The mean ADC values of bladder cancer, the bladder inflammation, the surrounding structures and the urine were (0.97±0.25)×10-3, (1.49±0.15)×10-3, (1.47±0.13)×10 -3 and (2.57±0.17)×10-3 mm2/s, respectively. There were significant differences between bladder cancer and the surrounding structures, urine, and bladder inflammation. The sensitivity, specificity and accuracy to distinguish Tis-T1 and T 2-T4 bladder cancer by DWI were 93.2%, 68.8% and 86.7%, respectively, which were 77.3%, 50.0% and 70.0% by conventional MRI sequences, respectively. There was a difference between DWI and conventional MRI sequences (P = 0.027). The mean ADC values of high- and low-grade urothelial carcinoma were (0.78±0.13)×10-3 and (1.14±0.21) ×10-3 mm2/s. There was a significant difference between high- and low-grade urothelial carcinoma (P < 0.01). By using the ROC (receiver operating characteristic) curve, given the ADC value of 1.04×10-3 mm2/s considered as a threshold for distinguishing high- and low-grade urothelial carcinoma, the sensitivity and specificity were 84.8% and 91.7%, respectively. Conclusion: DWI and ADC value are helpful in diagnosis, staging and grading of bladder cancer. Copyright © 2012 by TUMOR.
2.Investigation of Drug Manufacturers’Attitude to the National Assessment Programs of Sampling and Testing Policy
Lei WANG ; Yuwen CHEN ; Lian HUA
China Pharmacy 2016;27(18):2467-2469
OBJECTIVE:To provide reference for improving the national assessment programs of sampling and testing policy. METHODS:Questionnaire was designed to investigate the drug manufacturers’attitude and suggestions to the national assessment programs of sampling and testing policy;SPSS 18.0 software was adopted to analyze the data;a reliability analysis was conducted for the 17 variables in the table;and a principal component analysis was conducted for the topics with strong correlation. RE-SULTS:A total of 340 questionnaires were sent out,314 were effectively received with the effective rate of 92%. the total internal consistency reliability coefficient Alpha was 0.92;the awareness coefficient of enterprise on the evaluation of sampling and testing was 9.78;4.24 enterprises were relatively agree the evaluation of sampling and testing;the attitude coefficient of enterprise on the improvement of evaluation of sampling and testing was 13.39;and the average score of enterprise on the overall satisfaction scoring was 8.73. CONCLUSIONS:The drug enterprises show high overall recognition for the national assessment programs of sampling and testing policy,and willing to cooperate with the development,and hoping the policy can be improved.
4.Research advances on the protective effects and mechanism of salvianolic acids against ischemic heart disease
Awaguli DAWUTI ; Lian-hua FANG ; Guan-hua DU
Acta Pharmaceutica Sinica 2021;56(2):368-373
Salvianolic acids are the main water-soluble active compounds of
5.Research progress of natural drugs targeting mitochondrial autophagy against ischemic heart disease
Di-fei GONG ; Lian-hua FANG ; Guan-hua DU
Acta Pharmaceutica Sinica 2021;56(7):1872-1879
Ischemic heart disease (IHD), which has been considered to be exclusively caused by stenosis or occlusion of coronary artery, is a significant cause of morbidity and mortality worldwide. Mitochondrial dysfunction is the main pathological basis of ischemic heart disease and reperfusion injury, and moderate mitochondrial autophagy can selectively remove damage proteins and organelles to maintain intracellular homeostasis, so mitochondrial autophagy is important for maintaining the homeostasis of cardiomyocytes. Natural drugs from plants are widely used in ischemic heart disease. In recent years, more and more natural drugs have been proven to alleviate myocardial cell damage after ischemia/reperfusion through mitochondrial autophagy. This paper reviews the research progress of natural drugs from plants medicines regulating mitochondrial autophagy in the treatment of ischemia heart disease.
8.Expression of PKCθ in peripheral blood mononuclear cells of aplastic anemia patients and its effects on Th1 and Tc1 cells.
Xue-jing YANG ; Wei-hua ZHANG ; Xiu-lian ZHANG
Chinese Journal of Hematology 2012;33(11):951-953
Adolescent
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Adult
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Anemia, Aplastic
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blood
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Case-Control Studies
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Female
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Humans
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Isoenzymes
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metabolism
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Leukocytes, Mononuclear
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metabolism
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Male
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Middle Aged
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Protein Kinase C
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metabolism
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Protein Kinase C-theta
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T-Lymphocytes, Cytotoxic
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cytology
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Th1 Cells
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cytology
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Young Adult
9.Clinical Observation on Therapeutic Effects of Treatment of Diabetic Peripheral Neuropathy with Integrative Traditional Chinese and Western Medicine
Changxiu GUO ; Nanfang LOU ; Hua SUN ; Xu LIAN
International Journal of Traditional Chinese Medicine 2008;30(2):151-152
Objective To evaluate the clinical efficacy of Dan-Hong Injection and Methycobal combined therapy in treating diabetic peripheral neuropathy(DPN).Methods 72 cases of peripheral neuropathy and type 2 diabetes(conformed to ADA standard published in 1999)were randomly separated into an experimem group and a control group.Members in the experiment group were treated with Dan-Hong injection 20ml/day i.v.and Methycobal 500ug/day i.m.Members in the control group were treated with Methycobal only.At the end of 2 weeks'therapeutic courses,motor nerve conducdon velocity (MNCV)and sural nerve conduction velocity(SNCV)were evaluated.Results The overall effective rate was 92.1% in the experiment group and 67.6% in the control group,showing significant difference between the two groups(P<0.01).The post-therapeutic values of both SNCV and MNCV were significantly different from those of previous treatment.(P<0.01)The post-therapeutic values of SNCV and MNCV of the experiment group were also significantly different from those of the control group(P<0.05).No evidently relative adverse effects were observed in both groups.Conclusions Combined therapy of Dan-Hong injection and Methycobal was more effective in controlling the clinical activity of diabetic peripheral neuropathy.
10.Analysis on CYP2C19 polymorphism among patients with cardiovascular diseases in Dongguan area
Xianli HUA ; Gaifen LIAN ; Yali LEI ; Junjun PAN ; Hong SUI
International Journal of Laboratory Medicine 2017;38(5):606-608
Objective To investigate the distribution status of cytochrome CYP2C19 polymorphisms among patients with cardio-vascular diseases in Dongguan area .Methods A total of 1662 patients with cardiovascular diseases (CVD) in our hospital were se-lected .The peripheral blood was collected for extracting genomic DNA .The CYP2C19 genotype was detected by the polymerase chain reaction (PCR) combined with gene chip technology .The allele frequency and metabolic phenotype of CYP2C19 were com-pared between the CVD patients aged lower than 45 years and aged higher than 45 years old .Results Among 1662 cases ,713 cases (42 .90% ) were CYP2C19 extensive metabolizer ,740 cases(44 .52% ) were moderate metabolizer and 209 cases (12 .58% ) were poor metabolizer .The allele frequencies of CYP2C19﹡1 ,CYP2C19﹡2 and CYP2C19﹡3 were 65 .16% ,30 .08% and 4 .75% re-spectively .One hundred and four cases (40 .00% ) of extensive metabolisms were detected in the lower than 45 years old group ,104 cases(45 .38% ) of moderate metabolisms and 38 cases (14 .62% ) were detected .In the higher than 45 years old group ,609 cases (43 .44% ) of extensive metabolisms ,622 cases of moderate metabolisms and 171 cases(12 .20% ) of poor metabolism were detec-ted .The proportion of various genotypes had no statistical difference between the lower than 45 year old group and higher than 45 year old group(P>0 .05) .Conclusion Detecting CYP2C19 genotype for determining the genetic characteristics can evaluate the clopidogrel resistance risk and formulate the personalized anti-platelet therapeutic scheme .