Objective:To study the mechanism of apoptosis in hippocampus of the rats with disorder of cortical developments.Methods:The Rats with disorder of cortical developments were established by carmustine(BUCN).AT P0,P15,P30,P45,P60,the number of apoptosis cell and expression of Bax and Bcl-2 in hippocampus were detected by TUNEL,immunohistochemistry method and RT-PCR;then the detected outcomes were analyzed by computer micrograph analysis system in control group and model group.Results:At P15,P30,P45,P60,the numbers of apoptosis,Bax,Bcl-2 masculine cell and bcl-2 MRNA,bax MRNA in hippocampus in the model group were more than those in the control group.The ratio of Bax/Bcl-2 in model group was significantly higher than those in the control group(P

From Jeunary 1986 to June 1991 107 cases of carcinoma of stomach were found by gastroscopy, in which 100cases were proved pathologically by biopsy (93.5%).The biopsy of other 7 cases was negative and the diagnosis was confirmed lately by operation.gastroscopy and pathological characteristics and their clinical significane were discussed (103 cases of carcinoma of stomach cases of early stage carcinoma ventriculi case of pinpoint carcinoma).The authors suggested that ulcer, erosion, nodule and stiff surface on the depressed mucosa at the antrum be examined carefully by means of gastroscopy and biopsy be done in order to discover early stage carcinoma ventriculi and make a correct diagnosis.Being aware of these features in not only beneficial to the reasonable clinical treatmet but also helpful to the diagnosis of carcinoma of stomach.

Animals ; Antigens, Neoplasm ; genetics ; metabolism ; Biomarkers, Tumor ; genetics ; metabolism ; Brain Neoplasms ; metabolism ; Cell Adhesion Molecules ; genetics ; metabolism ; Digestive System Neoplasms ; metabolism ; Female ; Genital Neoplasms, Female ; metabolism ; Glioma ; metabolism ; Head and Neck Neoplasms ; metabolism ; Humans ; Immunotherapy ; Male ; Prostatic Neoplasms ; metabolism ; Signal Transduction

Pharmacodynamic (PD) studies play an important role in research and development of new traditonal Chinese medicines (TCMs). Phamacologic and toxicologic studies that aim to research drug ability can provide supporting data for the clinical trials and reduce the risk of clinical trials. In recent years, PD studies in TCMs are developing and progressing, but there are still some problems affecting the value of PD studies in the development of new TCMs. The value of PD studies depends on scientific and rational study design. This article summaries some defects in PD study design of new TCMs that are common in the application data, including defects in study type, testing targets, dosing, duration of administration, control group, testing time. This article also discusses some points of concern and specific requirements for PD study design of new TCMs.
Humans ; Medicine, Chinese Traditional ; methods ; Pharmacokinetics

Three-dimensional (3D) reconstruction of medical images constructs the 3D models of organs using 2D medical image serials, which provide intuitionistic visual information for medical workers. However, most people concern about the CT images reconstruction, only few people focus on the reconstruction of Ultrasound images. In this thesis, it introduces Insight Segmentation and Registration Toolkit (ITK) and The Visualization Toolkit (VTK) firstly. Then it presents the process of Ultrasound image serials using ITK and VTK. At last, it presents the outcome of the experiment. The results show that the reconstruction of Ultrasound images can also get good effect by using ITK and VTK.

OBJECTIVE:To reference clinical utilization of amikacin of a hospital.METHODS:By retrospective survey,the clinical information of 30 inpatients who were treated with amikacin and received plasma concentration monitoring from Apr.2006 to Feb.2010 were analyzed statistically in respect of primary disease,results of pathogen culture and drug sensitivity test,dosages and usage,plasma concentration,clinical efficacy,adverse drug reactions,etc.RESULTS:Clinical response rate was 55.2% and dosage of amikacin was small as well as measured value and reference value of plasma concentration.No adverse drug reaction was observed.CONCLUSION:The proportion of amikacin should be increased and plasma concentration monitoring of amikacin should be strengthened.Individual regimen is realized to improve effectiveness and safety of drug use.

Since the first launching of heart lung bypass machine in animal model in 1937,cardiopulmonary bypass(CPB) has become more and more consummate,but it still brings about many complications,including pulmonary,renal and hepatic dysfunctions.Among these complications,hepatic dysfunction plays an important role in the impairment after cardiac operations.The liver function indices such as aspartate aminotransferase,glutamate-pyruvate transaminase,bilirubin and alkaline phosphatase will step up obviously during 3 to 5 days after the operation.The physiological changes deteriorate patient's condition,and even influence the prognosis.This clinical phenomenon puzzles cardiac surgeons.All these demand us to answer the following questions: Is the cardiopulmonary bypass harm the liver? What's the mechanism? We review the different researches on hepatic injury after cardiopulmonary bypass.

Objective: To establish a HPLC method for the determination of paeoniflorin in Zhuifeng Tougu pill. Methods: HPLC was used with C18 column,CH3OH-H2O(30 :70)as mobile phase and detection wavelength at 230nm. Results: Paeoniflorin showed a good linearity in the range of 21.02～210.20?g. The average recovery was 100.7%and RSD was 2.0%. Conclusion: This method is simple and accurate with good reproducibility, and can be used for the quality control of Zhuifeng Tougu pill.

The migration of vascular endothelial cells plays an important role in the vascular growth, repair of wounds, and repair of vascular endothelial cells. At the same time, the migration of endothelial cells plays an important role in the progress of vascular diseases, especially atherosclerosis. Thus, the characteristics of endothelial cell migration and related factors are very important for us to understand the role of endothelial cells in the progress of physiology and pathology. In this article, the factors of promotion and inhibition, and blood flow, cytoskeleton and extracellular matrix are recommended in details. Furthermore, at the end of the article, we discuss the close relationship between endothelial cells and repair of vascular endothelial cells, even new vascular growth in the plaques of atherosclerosis.

Lung transplantation is now considered one of the effective management of end stage pulmonary diseases. This review presents the recent progress of primary graft failure in lung preservation, such as low temperature, ventilation, etc. The purpose is to provide some valid methods in clinical practice.