Esophagoplasty ; methods ; Esophagus ; surgery ; Humans ; Reconstructive Surgical Procedures ; methods ; Stomach ; anatomy & histology ; surgery

Objective: To study the characteristics of mouse embryonic stem (ES) cell line R1 expressing green fluorescent protein (GFP) efficiently and stably. Methods: Four kinds of biological characteristics: growth curve, express level of alkaline phosphatase, karyotype and pluripotentiality of the subclones of R1 ES cell expressing GFP were observed and analysed. Results: No obvious differences between R1 ES cells and the 4 ES cell clones expressing GFP［ESG（+）］ on the proliferation speed, differentiation state and the ratio of normal karyotype were observed. Teratocarcinoma concluded 3 germinal layers could form after inoculating ESG(+) cells to nude mice. Conclusion: The expression of GFP may not make any detectable effect on the proliferation speed, differentiation state and the pluripotentiality of R1 ES cells. This research work ensures efficient utilization of GFP as a reporter gene tracing ES cell in vivo.

Objective To explore the application of multilevel growth model in community intervention research. Methods The data a-bout blood pressure collected through questionnaire at baseline and 6 months, 12 months after intervention were analysed through multilevel growth model to evaluate the effectiveness of hypertension self-management. Results The blood pressures between the two groups were statistical significance at baseline,and the changing trends also were different. Age could affect diastolic blood pressure (DBP) changing after controlling the other factors. Conclusion Multilevel growth model can analyze the longitudinal data acquired from intervention research flexibly,and the result is more reliable.

Essential hypertension, which is common in population, has an unclear etioligy and the medical therapy is predominantly used. In the last few years, some studies found that the blood pressure could be elevated with the pulsatile compression at the root entry zone（REZ） of the Ⅸ、Ⅹcranial nerve, which may considered to be one of the causes of essential hypertension. Neurovascular decompression moderates the pulsatile compression, and is to be a new method for the treatment of essential hypertension, especially for refractory hypertension. This article summarized the mechanism of neurovascular decompression for essential hypertension and the operative courses.

Early diagnosis of inflammation and properly interfere to improve the prognosis are very important.However,it is difficult to verify inflammation or differentiate inflammation from infection in clinic,especially in the condition of underlying inflammation.Currently,radiopharmaceuticals based on researches of pathophysiology progress of diseases are studied constantly.This review summarizes the potential inflammation imaging radiotracers and their mechanisms,potential clinical application value.

Objective To investigate the diagnostic value of hysteroscopy combined with B -ultrasonography for uterine incision diverticulum after secondary cesarean section . Methods From May 2012 to May 2013, hysteroscopy check was performed in 38 cases with a history of two times of caesarean sections . The hysteroscopic results and ultrasound images were analyzed retrospectively . Results Incision diverticulum was diagnosed in 34 cases by using diagnostic hysteroscopy combined with B-ultrasonography, with a diagnosis rate of 89.5%(34/38).There were 18 cases of mild diverticulum (47.4%), 16 cases of severe diverticulum (42.1%), and 4 cases of good healing (10.5%).Of the 16 cases of severe diverticulum , the distance from top to serosal surface was 1.6-6.0 mm, and the width was 3-17 mm.Preoperative transvaginal ultrasonography ( TVS) showed only 10 cases of uterine incision diverticulum in patients with severe diverticulum , providing a diagnosis rate of 26.3% (10/38).Compared TVS with hysteroscopy combined with B-ultrasonography , there was a significant difference (χ2 =31.091, P=0.000) in the diagnosis rate for uterine incision diverticulum . Conclusion Hysteroscopy combined with B-ultrasonography has a high diagnosis rate for the diagnosis of uterine incision diverticulum in patients with two times of cesarean sections , being worthy of clinical application as a minimally invasive examination method after cesarean sections .

Esophageal Neoplasms ; therapy ; Humans ; Neoadjuvant Therapy ; Preoperative Care

Objective To explore the correlative factors of asthma after bronchiolitis.Methods Clinical data from 115 hospitalized patients with bronchiolitis were collected from Jan.2002 to May 2003,including age,gender,incidence season,family asthma history,birth weight,eczema,severity of bronchiolitis,pathogen,total and specific immunoglobulin E(IgE),overweight,smoking during pregnancy and feeding patterns;and the data of respiratory tract infection and wheezing onset in those cases were collected by clinic sevice and telephone follow-up in post-discharge for 5 years;the pollution status in living environment(coal dust and mine especially) and animal contact history(especially cats and dogs) were recorded.And the independent effect of risk factors was obtained,Logistic regression models were created for each outcome variable.Results Thirty-nine(33.9%) cases of 115 children with brochiolitis developed into asthma.There were significant differences between asthma and non-asthma group in family asthma history,recurrent lower respiratory tract infection(LRTI) under 1 year old,onset age,severity extent and obesity(P =0,0,0.004,0.004,0.020,respectively).The factors that contribute to the risk of asthma onset were incidence season,severe bronchiolitis,parental asthma,eczema and recurrent LRTI(OR=0.49,3.82,6.12,3.76 and 3.14,respectively P=0.000 7,0.026 0,0,0.030 9,0.000 5).But,the factors of gender,birth weight,congenital heartdisease,pollution status in living envi-ronment,animal contact history,feeding patterns,smoking during pregnancy,age when occured bronchioliyis,blood IgE and overweight had not correlated with asthma after bronchiolitis.Conclusions Severe bronchiolitis,parental asthma,eczema and recurrent LRTI under 1 year old correlate with development of asthma after bronchiolitis,however,falling ill in winter may be a protective factor in the progression of airway inflammation.

Objective To explore the factors affecting severity of infants with bronchiolitis in hospital.Methods Data were collected from hospitalized case of bronchiolitis including age,gender,birth weight,incidence season,overweight,severity of bronchiolitis,clinical manifestation,pathogen,total and specific immunoglobulin-E (IgE),X-ray appearance,family asthma history,eczema,feeding patterns,congenital heart disease,countryside dwelling and length of stay.All cases were divided according severity extent into 2 groups.Then one-factor analytical method was used between 2 groups,and risk factors for these outcomes were identified by Logistic regression analysis.Results 1.Nearly 56%(506 of 904) of children were considered as obesity.2.The positive rate of the virus infection identified from 904 infants with bronc-hiolitis was 78.3%.Of them,the respiratory syncytial virus (RSV) infection accounted for 43.5%,cytomegalovirus (CMV) for 47.6%,coxsackie b virus (CBV) for 7.9%,Mycoplasma pneumonia (MP) for 1.8%,adenovirus (ADV) for 1.7% and epstein-barr virus (EBV) for 0.15%,respectively.3.There were notable differences between mild group and severe group in age,pathogen,birth weight,countryside dwel-ling,congenital heart disease and hospital day(?2=3.47,19.62,2.32,2.61,-4.71,69.23 P=0.001,0.006,0.02,0,0,0).There was a significant association between severe bronchiolitis and young age,low birth weight,congenital heart disease,countryside dwelling and hospital days (OR=0.840,0.542,15.412,5.305 and 1.173,respectively P=0.013 3,0.049 7,0.000 3,0,0.000 2).Conclusions 1.Obesity may have great risk for bronchiolitis.2.Except for RSV,CMV,CBV,ADV,MP and EBV all were pathogens associated with childhood bronc-hiolitis.3.The factors that contribute to increase risk of severe bronchiolitis are young age,low birth weight,congenital heart disease and countryside dwelling.And length of stay may estimate severity of infants with bronchiolitis in hospital.

Objective To analyze clinical characteristics and treatment experience for late complications after hysteroscopic transcervical resection of endometrium ( TCRE) . Methods Clinical data of 13 cases of late complications after hysteroscopic TCRE for dysfunctional uterine bleeding from January 2006 to June 2014 were retrospectively analyzed .A re-operation of hysteroscopic exploration and resection were required . Results The re-operation in the 13 patients found 6 cases of hematocele in uterine cavity (4 cases in the uterine horn, 2 cases in the uterine cavity) and 7 cases of focal or whole uterine endometrial hyperplasia (including 1 cases of PASS syndrome and 1 case of endometrial pathology of grade Ⅰatypical hyperplasia ) .Postoperative symptoms were relieved in 7 cases (53.8%).Laparoscopic total hysterectomy was performed in 3 cases of adenomyosis complicated with secondary progressive dysmenorrhea (23.1%), 2 cases of endometrial proliferation associated with a breast cancer history (15.4%), and 1 case of endometrial atypical hyperplasia grade Ⅰ (7.7%). Conclusion After hysteroscopic TCRE, residual functional endometrium or future regeneration of endometrium may lead to late complications , which need further clinical diagnosis and treatment .