The characteristic,theory,fiber coatings,method development of solid-phase microextraction and its coupling with other analytical technologies were reviewed with 59 references.

Objective To explore the correlative factors of asthma after bronchiolitis.Methods Clinical data from 115 hospitalized patients with bronchiolitis were collected from Jan.2002 to May 2003,including age,gender,incidence season,family asthma history,birth weight,eczema,severity of bronchiolitis,pathogen,total and specific immunoglobulin E(IgE),overweight,smoking during pregnancy and feeding patterns;and the data of respiratory tract infection and wheezing onset in those cases were collected by clinic sevice and telephone follow-up in post-discharge for 5 years;the pollution status in living environment(coal dust and mine especially) and animal contact history(especially cats and dogs) were recorded.And the independent effect of risk factors was obtained,Logistic regression models were created for each outcome variable.Results Thirty-nine(33.9%) cases of 115 children with brochiolitis developed into asthma.There were significant differences between asthma and non-asthma group in family asthma history,recurrent lower respiratory tract infection(LRTI) under 1 year old,onset age,severity extent and obesity(P =0,0,0.004,0.004,0.020,respectively).The factors that contribute to the risk of asthma onset were incidence season,severe bronchiolitis,parental asthma,eczema and recurrent LRTI(OR=0.49,3.82,6.12,3.76 and 3.14,respectively P=0.000 7,0.026 0,0,0.030 9,0.000 5).But,the factors of gender,birth weight,congenital heartdisease,pollution status in living envi-ronment,animal contact history,feeding patterns,smoking during pregnancy,age when occured bronchioliyis,blood IgE and overweight had not correlated with asthma after bronchiolitis.Conclusions Severe bronchiolitis,parental asthma,eczema and recurrent LRTI under 1 year old correlate with development of asthma after bronchiolitis,however,falling ill in winter may be a protective factor in the progression of airway inflammation.

Objective To explore the factors affecting severity of infants with bronchiolitis in hospital.Methods Data were collected from hospitalized case of bronchiolitis including age,gender,birth weight,incidence season,overweight,severity of bronchiolitis,clinical manifestation,pathogen,total and specific immunoglobulin-E (IgE),X-ray appearance,family asthma history,eczema,feeding patterns,congenital heart disease,countryside dwelling and length of stay.All cases were divided according severity extent into 2 groups.Then one-factor analytical method was used between 2 groups,and risk factors for these outcomes were identified by Logistic regression analysis.Results 1.Nearly 56%(506 of 904) of children were considered as obesity.2.The positive rate of the virus infection identified from 904 infants with bronc-hiolitis was 78.3%.Of them,the respiratory syncytial virus (RSV) infection accounted for 43.5%,cytomegalovirus (CMV) for 47.6%,coxsackie b virus (CBV) for 7.9%,Mycoplasma pneumonia (MP) for 1.8%,adenovirus (ADV) for 1.7% and epstein-barr virus (EBV) for 0.15%,respectively.3.There were notable differences between mild group and severe group in age,pathogen,birth weight,countryside dwel-ling,congenital heart disease and hospital day(?2=3.47,19.62,2.32,2.61,-4.71,69.23 P=0.001,0.006,0.02,0,0,0).There was a significant association between severe bronchiolitis and young age,low birth weight,congenital heart disease,countryside dwelling and hospital days (OR=0.840,0.542,15.412,5.305 and 1.173,respectively P=0.013 3,0.049 7,0.000 3,0,0.000 2).Conclusions 1.Obesity may have great risk for bronchiolitis.2.Except for RSV,CMV,CBV,ADV,MP and EBV all were pathogens associated with childhood bronc-hiolitis.3.The factors that contribute to increase risk of severe bronchiolitis are young age,low birth weight,congenital heart disease and countryside dwelling.And length of stay may estimate severity of infants with bronchiolitis in hospital.

Cholangiocarcinoma is a highly malignant tumor which originates from bile duct epithelial cells, and its invasion and metastasis is an important factor affecting the prognosis of patients and the main causes of the high mortality.Epithelial-mesenchymal transition is a reversible biological process of transition from epithelial phenotype to stromal cell phenotype, which is closely related to tumor invasion and metastasis.The microRNA is an endogenous non-coding RNA with a sequence of 20~25 nucleotides, which participates in the occurrence and development of tumor, especially in the progress of tumor invasion and metastasis by complementary base pairing with the 3'-untranslated region (3'-UTR) of the target gene at the post-transcriptional level.Herein, we present the mechanism of microRNA regulating epithelial-mesenchymal transition and its effect on the invasion and metastasis of cholangiocarcinoma.

Objective To explore the effect of psychological intervention on the quality of life in patients with advanced non-small cell lung cancer chemotherapy. Methods A total of 60 patients with advanced non-small cell carcinoma chemotherapy in our hospital from January 2015 to January 2017 were enrolled and divided into two groups according to different intervention methods. The control group was treated with Shengmai injection alone. The observation group was treated with psychological intervention + compound Kushen injection.The changes of scores of depression (SDS), anxiety (SAS) and pain (VAS), quality of life (QLQ-CCC) score and adverse reactions were recorded and analyzed before and after intervention in two groups. Results The clinical intervention effect of the observation group was better than that of the control group, the stability of the tumor focus, the degree of pain, the quality of life and other aspects were better than the control group, the difference between the two groups was statistically significant (P<0.05). Conclusion Patients with advanced non-smal cel lung cancer chemotherapy choose psychological intervention +compound sophora injection combined treatment effect is remarkable, which can effectively improve the patient's clinical symptoms and quality of life, is worth widely used clinically.

Objective To investigate precaution effect of ephedrine on protamine's untoward reaction. Meth-ods Forty patients, who were scheduled to have mitml valve replacement operation were randomly divided into two groups: ephedrine group(group A, n = 20) and control group (group B, n = 20). 50ml solution, mixed with protamine and 5% glucose,was injected through internal jugular vein in five minutes to neutralize the effect of heparin. In group A,3mg ephedrine was added into neutralization solution while in group B nothing was added. Arterial pressure(AP) , heart rate(HB) peripheral hemoglobin oxygen saturation(SpO2) and airway resistance(AR) were measured before injection and 1,3,5,10 minutes after injection in both groups. Results In group A, the AP, HR, SpO2 and AR had no significant difference before and after injection (P >0.05) ;while in 6 eases of group B, the AP, HR, SpO2 and AR have significant difference before and after injection. Conclusion Ephedrine can significantly improve protamine's untoward reaction, and neutralize the effect of heparin.

Objective To investigate the changes and clinical signficance of nitric oxide(NO) and endothelin(ET) in children with lymphoma.Methods NO and ET of 39 cases with lymphoma and 33 normal controls were examined by using nitrose reductase method and RIA method.Results Compered to normal controls,NO and ET were higher in children with lymphoma;smilar to normal controls in complete remission(CR) and partial remission(PR);relapse cases were higher than remission.Conclusions NO and ET could have relation to lymphoma in children,testing the changes of NO and ET have signficanc to the assistant diagnosis,observeing effect and judging prognosis.

Objective: To provide anatomic data for management of pterygopalatine fossa lesions via endoscopic transnasal approach. Methods: Fifteen (30 sides) formalin-preserved human skulls were subjected to microsurgical dissection to evaluate the exposure scopes of 3 different endoscopic surgery approaches: endonasal middle meatal transpalatine approach, endonasal middle meatal transantral approach, and endonasal inferior turbinectomy transantral approach. Meanwhile, 20 (40 sides) dry skulls were dissected and the related measurements were obtained. Results: The 3 approaches allowed for different exposure of the pterygopalatine fossa structure. The mean distances from foramen rotundum to anterior nasal spur, superior orbital fissure, and optic canal were (61.86±3.67)mm,(3.56±0.75) mm, and (11.23±1.24) mm, respectively. Conclusion: The 3 endoscopic approaches are safe and effective for resection of different pterygopalatine fossa lesions and foramen rotundum serves as a landmark for safe management of the pterygopalatine fossa.

Biliary atresia(BA) is a common reason for obstructive jaundice in children.Pathogenesis of BA is unclear,and the prognosis is poor.Over the last 2 years,gene mutation,viral infection and autoimmune are considered to be the most possible reason for BA;Stool co-lor card has shown favourable perspective in early screening;Kasai is also the first choice for treatment of BA,which progressed in laparoscopic operation and robot surgery.Corticosteroids after surgery is still disputing.Now,liver transplantation is the only way for BA and cirrhosis.This article gave a review on the pathogenesis,diagnosis and treatment of BA.

OBJECTIVE: To investigate the effects of puerarin on impaired endothelium-dependent relaxation induced by glycosylated bovine serum albumin (GBSA) in rabbit thoracic aorta and its mechanisms. METHODS: The rings were incubated with GBSA for 30 min to induce endothelial dysfunction, and with puerarin(0.25, 0.5 and 1 g · L-1), A-nitro-L-arginine methyl ester (L-NAME, 30 νmol · L-1), and indomethacin(10 μmol · L-1) to investigate the protective effect of puerarin on impaired vascular endothelial function elicited by GBSA, and the effect of L-NAME and indomethacin on the protective effect of puerarin. Moreover, the content of nitric oxide (NO) and malonaldehyde (MDA) and the activity of superoxide dismutase(SOD) in the rings were measured. RESULTS: Exposure of aortic rings to GBSA (200 mg · L-1) for 30 min resulted in a significant inhibition of endothelium-dependent relaxation, but had no affect on endothelium-independent relaxation. GBSA significantly decreased the level of NO and the activity of SOD but hugely increased the content of MDA in vascular tissues. Pre-incubation of aortic rings with puerarin markedly attenuated the inhibition of endothelium-dependent relaxation induced by GBSA. This protective effect of puerarin (1 g · L-1) was partially inhibited by L-NAME but not by indomethacin. Puerarin obviously increased NO level and SOD activity but evidently decreased MDA content in rings. CONCLUSION: Puerarin can protect against vascular endothelial dysfunction caused by GBSA and its mechanisms may be related to enhancing NO synthesis and its anti-oxidation.