0.05). But the Wuling Sang could obviously decrease serum TG content than Simvastatin(P0.05). The adverse drug reactions were stomach discomfort(4 patiens in SWS) group, 5.71%), abdominal discomfort(2 patiens in Simvastatin group, 4.55% ) and sleeplessness(5 patiens in Simvastatin group, 11.4%) . CONCLUSION: The results show the TCM drug SWS is effective and softy on treating the patients with primary hyperlipemia.

Objective To investigate the clinical effects of the surgery of mastoid muscle periosteum valvuloplasty after modified radical mastoidectomy treatment of cholesteatoma otitis media.Methods 52 cases (52 ears)cholesteatoma otitis media patients for the observation group were collected.The patients in the observation group were treated by mastoid muscle periosteum valvuloplasty after modified radical mastoidectomy.49 cases (49 ears) for the control group were treated by concomitant mastoidectomy and the classic conchaplasty cavity angioplasty.Results Patients were followed up for 1 year.38 ears were dry in control group(77.6％),8 ears were recurrenced(16.3％),regular clean-up of surgical cavity at least 3 months postoperative.50 ears were dry in observation group (95.2％),dry ear rate was significantly higher (x2 =12.374,P =0.000) ;1 ears was recurrenced(2.4％),the recurrence rate was significantly reduced (x2 =11.966,P =0.000) ; Operative cavity after complete epithelialization no longer need to be cleaned regularly; No postoperative complications (such as periosteal flap or pinna cartilage of the mastoid muscle necrosis,facial paralysis,dizziness)were occurred,the prognosis was good.Conclusion The surgery of mastoid muscle periosteum valvuloplasty after modified radical mastoidectomy treatment of otitis media with cholesteatoma is safe and effective,which is worthy of promotion.

The lack of pulmonary surfactant(PS) and the immature of lung structure can cause neonatal respiratory distress syndrome (NRDS).Pulmonary surfactant,noninvasive ventilation,such as nasal continuous positive airway pressure(nCPAP) and the InSurE technique(intubation-surfactant-extubation) are the important methods for the management of NRDS.In this paper,the application and the research progress of the three strategies for NRDS,especially the clinical advantages and progress of InSurE technique,are summarized as follows.

The fetal inflammatory response syndrome (FIRS) is a state of activation of the innate immune system in the fetus body and is a kind of neonatal inflammation for 72 hours after birth.It is defined as the following items ＞ 2:(1) breathing too fast (＞ 60 times/min),accompanied by breathing difficulties or with oxygen desaturation; (2) temperature instability ＞ 37.9 ℃ or ＜ 36 ℃ ; (3) capillary filling time ＞ 3 s;(4) the white blood cell count ＞34 × 109/L,or ＜4 × 109/L; (5) CRP ＞ 10 mg/L; (6) IL-6 or IL-8 ＞70 g/ml;(7) gene testing of 16S rRNA for PCR is positive.FIRS can be induced by infectious and non-infectious factors.In FIRS,fetal immune system is over-activated to the external violation,which causes uncontrolled release of inflammatory mediators and cytokines.A variety of inflammatory mediators and cytokines directly or indirectly activate the coagulation system and interfere with the body's anticoagulation system,which induces the coagulation disorders.Multiple organ systems involved in the inflammatory response throughout this process.FIRS can lead to premature delivery,perinatal death,cerebral white matter damage,necrotizing enterocolitis,and affect fetal lung maturity and multi-organ damage.In order to reduce fetal injury,it is necessary for appropriate treatment and prediction of the FIRS.

This article found that the actual situation about humanities education of medical postgraduates is not optimistic through investigation of the situation on humanities education of the postgraduates in Zunyi Medical College. Some prominent problems existed. This article also proposed some practical suggestions to solve the problems based on the analysis of this phenomenon, so as to improve the humanities education of medical postgraduates and cultivate postgraduates to be medical workers with full humanistic care.

Insulin is one of the most important hormonal regulators of metabolism. Since the diabetes patients increase dramatically, the chemical properties, biological and physiological effects of insulin had been extensively studied. In last decade the development of NMR technique allowed us to determine the solution structures of insulin and its variety mutants in various conditions, so that the knowledge of folding, binding and stability of insulin in solution have been largely increased. The solution structure of insulin monomers is essentially identical to those of insulin monomers within the dimer and hexamer as determined by X-ray diffraction. The studies of insulin mutants at the putative residues for receptor binding explored the possible conformational change and fitting between insulin and its receptor. The systematical studies of disulfide paring coupled insulin folding intermediates revealed that in spite of the conformational variety of the intermediates, one structural feature is always remained: a "native-like B chain super-secondary structure", which consists of B9-B19 helix with adjoining B23-B26 segment folded back against the central segment of B chain, an internal cystine A20-B19 disulfide bridge and a short α-helix at C-terminal of A chain linked. The "super-secondary structure" might be the "folding nucleus" in insulin folding mechanism. Cystine A20-B19 is the most important one among three disulfides to stabilize the nascent polypeptide in early stage of the folding. The NMR structure of C.elegans insulin-like peptide resembles that of human insulin and the peptide interacts with human insulin receptor. Other members of insulin super-family adopt the "insulin fold" mostly. The structural study of insulin-insulin receptor complex, that of C.elegans and other invertebrate insulin-like peptide, insulin fibril study and protein disulfide isomerase (PDI) assistant proinsulin folding study will be new topics in future to get insight into folding, binding, stability, evolution and fibrillation of insulin in detail.

To investigate the cause,pathogenesis,monifestation and treatment of osteoporosis and bonic arthritis.Bonic arthristis was not the osteoposis.Osteoporosis and bonic arthristis may occured simultaneously.In order to treat the conditions correctly,differentiated diagrosis should be noticed clinically.

Objective To analyze clinical characteristics, hospital treatment, complications and mortality differences of the patients with acute myocardial infarction (AMI) by conventional treatment. Methods Retrospective analysis of 426 cases of AMI patients, aged 20-103 years [mean age(67. 82 ±0. 56) years] ,male 261 cases,female 165 cases, the general condition of patients were recorded, history features, diagnosis, treatment, complications and prognosis. Comparison of elderly(≥70 years) and non-elderly(＜70 years) ,male and female patients with clinical features, treatment, complications and mortality differences. Results Compared with the non-elderly patients with AMI,elderly patients had significantly lower smoking history(P＜0.05) ,female ratio,history of old myocardial infarction rate, cardiac function≥Killip Ⅲ level was significantly higher(all P＜0.05). During hospitalization in patients of different ages diuretics,β blockers, digitalis, clopidogrel and other drugs, application of a significant difference (all P＜0.05). Elderly AMI patients with complete left bundle branch block,atrioventricular block,atrial fibrillation,the incidence was higher than non-elderly patients (all P＜0.05) ,but no difference in mortality between the two groups (P＞0.05). Female patients than male patients with age of onset was significantly (P＜0.05) , and the smoking rate is low,but clearly the high rate of angina died(P＜0.05) ,sub-poor cardiac function after myocardial infarction(P＜0.05). Application of cardiac drugs in addition to nutrition than men,female patients, the rate of other conventional therapy,no difference in both complications and prognosis(all P＞0.05). Conclusion AMI was the major diseases threatening human life,different age had its own clinical characteristics of patients,and it could not be impacted by gender.

[Objective] To explore the levels of folic acid(FA), vitamin(Vit) Bl2 and homocysteine(Hcy) of gestational diabetes mel itus(GDM) patients and the relation among them. [Method] Select 40 GDM cases from our hospital diagnosed with oral glucose tolerance test(0GTT) and 40 healthy pregnant women in the same period, take circulation enzymic method to test Hcy concentration, and take chemiluminescence to measure FA and VitBl2. [Result] GDM patients’Hcy level is in negative relation with VitBl2 and FA levels. Comparing GDM patients’serum Hcy,VitBl2 and FA with normal pregnant group,Hcy level is raised much(P<0.01), FA and VitBl2 are reduced markedly(P<0.01). In later period of GDM pregnancy, they have high Hcy, lack of FA and Vit-Bl2; the pregnancy hypertension shal strengthen the intake of VitBl2 and FA, as wel as have periodical supervision, to reduce Hcy level and decrease the side effects on fetus and injury to pregnant women’s great vessels and micro-vessels.

Objective To identify the researchers' productivity,the citation states and the grant patterns of the Chinese Journal of Hepatobiliary Surgery through citation analysis.Methods Articles published in the Chinese Journal of Hepatobiliary Surgery between 2009 to 2012 were collected from the Chinese Science Citation Database.These articles were analyzed with statistics and bibliometrics.The indicators included cooperation degree,core authors,distribution of grant,authors' origin and citation states.Results Of the 1321 papers which were published by the journal during the study period,505 papers were cited 923 times.Of the 1111 first authors,957 (86.14％) published one paper only.There were 154 core authors (13.86％ of first authors),and they published 364 papers (27.55％).There were 14 high-productivity institutes,which published 299 papers (22.63％).According to the geographical distribution,Beijing ranked first with 190 (14.38％) papers.The second was Shanghai with 156 (11.81％),the third was Jiangshu province with 134 (10.14％),the fourth was Zhejiang province with 132 (9.99％),and the fifth was Guangdong province with 101 (7.65 ％) papers.1262 papers were completed by collaborate teams of more than one author.The cooperative rate was 95.53％ and the cooperative degree was 4.67.361 papers were funded by 553 supporting foundations.Conclusions The authors of the Chinese Journal of Hepatobiliary Surgery were geographically widely distributed.Some were core authors with high-productivity.They were highly cited and with good cooperation degree.Beijing,Shanghai,Jiangshu,Zhejiang and Guangdong provinces play important roles in research on hepatobiliary surgery.