Objective To investigate the effect of recombinant rat augmenter of liver regeneration (rrALR) on the biological activities of rat glomerular mesangial cell(GMC) with or without IL-1?. Methods GMC proliferation and the level of TGF-? protein were determined by 3H-TdR incorporation and ELISA. Results The stimulating activity of IL-1? to rat GMC was enhanced by rrALR with the concentrations from 0.005 pg/ml to 0. 1ng/ml. But rrALR could not promote the proliferation of GMC without IL-1?. Conclusion rrALR may be a new mediator of inflammation, which promote proliferation and TGF-? secretion in rat GMC stimulated by IL-1?.

Objective To explore the clinical characteristics and effects of thrombolytic and anti coagulation treatment in over 70 year old patients with pulmonary thromb embolism(PTE). Methods The clinical findings, diagnostic techniques, effects of thrombolytic therapy and anti coagulation in 58 over 70 year old patients with PTE were analyzed retrospectively. Results Deep venous thrombosis (DVT) in lower limbs (74 1%) was the most common thrombolism prone factors in our study. Limitation of movement and long term stay in bed due to a variety of causes were in the next place. The clinical findings were atypical, associated with a false diagnostic ratio of 46 5% in elderly patients with PTE. Undefined causal and different degree of dyspnea along with sudden and persistent hypoxemia were the main characteristics. Spiral CT and radioactive nuclear ventilation perfusion scan were important methods for diagnosis of PTE with positive finding of 95 7% and 62 5%, respectively. The effective and cure rate of thrombolytic therapy combined with anti coagulation, anti coagulation and anti platelet therapy was 91 3%, 75 8%, 0 and 47 8%, 17 2%, 0, respectively. Conclusions The most common risk factors of PTE in the elderly was DVT and the long term stay in bed or stay without active moving. The clinical symptoms were not only atypical but also variable. Thrombolytic with anti coagulation therapy is safe and effective, but anti platelet coagulation alone is not benefit.

BACKGROUND:Presently used pulmonary vein antrum location methods mainly performed by three-dimensional electroanatomy combined with X-ray image or CT image fusion.These methods conducted vein antrum location and ablation by anatomy instructions.It is still poorly understood whether the ablation hit the key part of atrial fibrillation.OBJECTIVE:To explore the feasibility of catheter ablation guided by three-dimensional electroanatomic mapping system in conjunct with pulmonary vein antrum potential in patients with atrial fibrillation.DESIGN,TIME AND SETTING:The verification clinical study was performed at the Department of Cardiology of Jinan Fourth People's Hospital and Hebei People's Hospital from March 2007 to June 2009.PARTICIPANTS:Fifty-one patients with drugs refractory,paroxysmal atrial fibrillation were included.METHODS:All patients underwent circumferential pulmonary vein antrum ablation guided by three-dimensional electroanatomic mapping system (CARTO) in conjunct with pulmonary vein antrum potential with the endpoint of electrical isolation.Relevant parameters and ablation success rate were observed.MAIN OUTCOME MEASURES:Procedure-related parameters,such as procedure duration,fluoroscopy duration,cumulative success rate and complication were observed.RESULTS:Pulmonary veins were isolated in all 51 patients.The mean procedure duration,fluoroscopy time and radiofrequency ablation duration are respectively (207±36.7) minutes,(38.2±14.3) minutes,(56.4±15.7) minutes.After (17.5±3.8)-months follow-up,forty (78.4%) patients did not have recurrence of atrial fibrillation,atrial flutter or atrial tachycardia.No severe procedure-related complication had happened.CONCLUSION:Pulmonary vein antrum potential can be used as a landmark to define pulmonary vein antrum,that combined with CARTO system to guide pulmonary vein antrum ablation is effective,safety and feasible for catheter ablation of atrial fibrillation.

The germ cell lineage in the mouse is established during gastrulation;sex determination is achieved after germ cell migrating to the site of the future gonads. Germ cells proliferate indefinitely when cultured in vitro . Human embryonic germ(EG) cells have been recently established;these immortalized EG cells are chromosomally stable and pluripotent, raising the hope that their differentiation can be directed to specific cell types, which may be of value in the clinical treatment of degenerative diseases.

Objective:To study the analgesic effect of etomidate infusion for radical mastectomy in breast cancer and its effect on hemodynamics and inflammatory mediators.Methods:From August 2015 to July 2016,84 patients with radical mastectomy were divided into the observation group and the control group according to the order of admission.The observation group was given continued infusion of etomidate,the control group was given continued infusion of propofol.The visual analogue (VAS) at different time points after operation,diastolic blood pressure (DBP),systolic blood pressure (SBP) and heart rate (HR) at the time ofextubation and serum IL-2,IL-10,IL-12 levels at 72 hours after operation were compared between two groups.Results:At the time of extubation,the DBP,SBP and HR were significantly lower in the observation group than in the control group (P＜0.05).The VAS scores of observation group were significantly lower than those of the control group at 1,5,10,24 and 48 hours after operation (P ＜0.05).The serum levels ofIL-2 in the observation group was significantly higher than that of the control group (P＜0.05),IL-10 and IL-12 levels were lower than control group (P＜0.05).Conclusion:Sustained infusion of etomidate had good analgesic effect in the treatment of breast cancer patients underwent radical mastectomy with little effect on the hemodynamics and inflammatory mediators.

Objective To observe the effect of constraint-induced movement therapy combined with motor imagery on unilateral spatial neglect (USN) in stroke patients. Methods Fifty stroke patients with USN were randomly divided into a treatment group ( n = 27 ) and a control group ( n = 23 ). Both groups received routine physical therapy training, including with the Bobath technique and low frequency electrotherapy, while the treatment group received constraint-induced movement therapy and motor imagery in addition. All the patients were assessed with 4 scales of the regular USN assessment ( cancellation tests, line bisection tests, clock drawing tests,copying drawing tests) and with the Barthel index (BI) before and after 8 weeks of treatment. Results After 8 weeks of treatment, both groups' average USN assessments and Barthel indices improved significantly. Furthermore, both the USN results and the Barthel index scores in the treatment group were, on average, significantly better than those in the control group. Conclusion For USN stroke patients, constraint-induced movement therapy combined with motor imagery improves the symptoms of USN and ADL ability significantly better than routine physical therapy treatment alone.

Objective To determine the effect of lean mass and fat mass on bone mineral (BMD) in postmenopausal women of different age groups.Methods A total of 287 postmenopausal women (aged 50 years and over) participated in this study.Individuals were divided into 4 age groups by decades:group 1,50-59 yr (82 cases); group 2,60-69 yr (73 cases); group 3,70-79 yr (66 cases); group 4,80 years and over (66 cases).The BMD of lumbar spine (L2-4),left femoral neck and total hip were measured by dual-energy X-ray absorptiometry.Fat and muscle mass were analyzed by its analysis function and the percent of fat mass and muscle mass were calculated.Then data were processed by the SPSS 18.0 programme.Results One linear regression analysis showed that the L2-4 BMD was significantly associated only with body composition of trunk,while femoral neck (FN) and total hip (TH) BMD were respectively associated with body composition of different parts in group 1.L2-4 BMD was significantly associated only with trunk fat mass and muscle mass (r=0.27,0.25,both P＜ 0.05),but there were no relationships between BMD of FN and TH and body composition of different parts in group 2.The BMD of L2-4,FN and TH were significantly respectively associated with body composition of different parts in group 3 and 4.Conclusions In postmenopausal women,fat mass and muscle mass play an important role in BMD.In postmenopausal women aged 60-69 yr,the body composition has a minimal impact on BMD,and hormone replacement therapy may be the best for osteoporosis prevention and treatment in this period.Different exercise and loosing of weight in postmenopausal women of different ages can prevent osteoporosis and fragility fractures.

To develop a safe and effective new generation vaccine for IRKV-THChina12 prevention, we constructed a non-replicative recombinant human adenovirus carrying the IRKV-THChina12 G gene, named as rAd5-IRKV-G. The IRKV-THChina12 G protein expressed by the recombinant human adenovirus in 293AD cells was detected by western blot and indirect immunofluorescence test. To evaluate the immunogenicity of the recombinant, mice were immunized with rAd5-IRKV-G by intramuscular (i. m.) or intraperitoneal (i. p.) route and with non-exogenous gene expressing wild type adenovirus wt-rAd5 as a control. Results showed that the rAd5-IRKV-G could induce continuous and statistically significant (P ≤ 0.05) anti-IRKV neutralizing antibody (NA) production in immunized mice by i. m. or i. p. route. In particular, no significant difference (P > 0.05) of the NA titers between the two administration routes were observed, that provides an alternative choice for animal immunization method in the future application.
Adenoviruses, Human ; genetics ; physiology ; Animals ; Antibodies, Neutralizing ; immunology ; Antibodies, Viral ; immunology ; GTP-Binding Proteins ; genetics ; immunology ; Gene Expression ; Genetic Vectors ; genetics ; physiology ; Humans ; Immunization ; Lyssavirus ; enzymology ; genetics ; immunology ; Mice ; Rhabdoviridae Infections ; immunology ; virology ; Viral Proteins ; genetics ; immunology ; Virus Replication

Allicin,which contains rich sulfide ,is provided for prevention and treatment of tumor ,anti in-flammation,prevention and treatment of cardiovascular diseases ,improving immunity and so on .Studies show that allicin can not only prevent many types of cancer ,but also has obvious inhibitory effect on tumor .The mechanism is related with antioxidant effects ,reducing the invasion of tumor cells ,inhibiting the proliferation of tumor cells , inducing tumor cell apoptosis and directly killing the tumor cells .This paper reviews the research progresses on antitumor allicin .