Objective To investigate the clinic diagnosis value of CT scan and the determination of serum NSE, Cyfra21-1 for SCLC. Methods ① The CT findings and pathologic basis of 220 cases with SCLC were analyzed retrospectively.② Between February 2000 and December 2001, 120 consecutive patients with pulmonary lesions by CT scan, which suspiciously suffered lung cancer, were studied prospectively. Serum samples were obtained by venous puncture from all patients 1 week prior to surgery or puncture biopsy. The serum concentration of NSE, Cyfra21-1 were determinated using ELISA. According to the pathologic result, the diagnostic value of CT scan, NSE and Cyfra21-1 were evaluated.Results ① Most CT findings of SCLC were central and spindly or eggplant-shape mass, hilar and/or mediastinal lymph nodes enlargement.The obstructive pulmonary lesions were found rarely.② Using CT scan, NSE and Cyfra21-1 alone, the sensitivity were 75%,72.5% and 32.5%,the specificity were 73.75%, 83.75% and 48.75%,the accuracy were 74.16%, 81.67% and 43.33% respectively. With combination of CT scan and NSE, the diagnostic accuracy was 78.3%, and the sensitivity was 87.5%. However, CT scan combined with Cyfra21-1 can not be improve the diagnostic value. Conclusion SCLC has specific CT findings. The sensitivity of CT scan was 75%, combinated with NSE, its accuracy was higher(78.3%), and the sensitivity was 87.5%.

OBJECTIVETo evaluate the influence of naringin on PMMA-induced osteoclastic bone resorption using the mouse air sacs model.

METHODSTotal 48 female Balb/c mices with the age of 8 to 10 weeks were chosen in the study. Air were injected into the back in 32 mices and formed the air sacs, 6 d later, the skulls (originated from other 16 mices) were implanted to the air sacs. Thirty-two animals were divided into naringin treatment group (with 2 concentrations of 150 mg/kg and 30 mg/ kg) , DMSO group and PBS blank group, 8 animals in each group. Polymethylmethacrylate (PMMA) particles were injected into the air sacs in naringin treatment groups and DMSO group so as to irritate inflammatory reaction. Naringin with 2 concentrations of 150 mg/kg and 30 mg/kg were dissolved in DMSO of 0.2 ml, and were injected into air sacs, respectively. In PBS black group, no stimulation with PMMA particles, only injected PBS, and in DMSO group, injected DMSO without naringin. Tartrate resistant acid phosphatase (TRAP), Ca2+ release, modified Masson stain and histological analysis were performed on the 7th day after stimulation.

RESULTSCompared with DMSO group, naringin treatment group's cellular infiltration decreased (P < 0.01); concentration of 150 mg/kg was better than that of concentrations of 30 mg/kg (8.90 ± 1.75 vs 15.23 ± 1.86). Naringin can decrease calcium release in the lavage of the air sacs bone resorption model, especially obvious in naringin with concentration of 150 mg/kg. Naringin can ameliorate the inflammatory reaction and the subsequent bone resorption (including bone collagen loss, TRAP positive cells amount and so on) in air sacs with bone implant and PMMA particles. Naringin with concentration of 150 mg/kg appeared to be an optimal dosage to deliver the therapeutic effects.

CONCLUSIONNaringin inhibits PMMA-induced osteoclastogenesis and ameliorates the PMMA-associated inflammatory reaction and the subsequent bone resorption.

Animals ; Disease Models, Animal ; Female ; Flavanones ; therapeutic use ; Mice ; Mice, Inbred BALB C ; Osteoclasts ; drug effects ; physiology ; Osteolysis ; chemically induced ; prevention & control ; Polymethyl Methacrylate ; toxicity

It is very difficult for stroke patients to complete the action of squatting-standing because their equilibrium function ability has been seriously declined. It was necessary, therefore, to do a deep research on the action of human squatting-standing and to set up an accurate model and simulation. In our modeling research, the movements of upper limbs and head was neglected, and a seven-segment model was developed to establish the coordinate system of human squatting-standing action. It calculated the knee joint moment and hip joint moment during squatting and standing by utilizing Lagrange method, and then simulated this mathematical model by utilizing Matlab. Geometric model of human squatting-standing was developed and simulated in ADAMS which proved that the established Lagrange model was reasonable. It would also provide significant theoretical references for further study and development of squatting-standing rehabilitation training equipment.
Biomechanical Phenomena ; Computer Simulation ; Hip Joint ; Humans ; Knee Joint ; Models, Theoretical ; Movement ; Posture ; Upper Extremity

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Carbon Monoxide Poisoning ; enzymology ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Myocardium ; enzymology ; Young Adult

Objective To analysis The clinical efficacy of XiaoErKang granule combined with MAMIAI to 98 children with autumn ped-iatric diarrhea.Methods98 children with diarrhea from January 2015 to December 2015 were divided into 49 cases with treatment group and 49 cases with the control group.The control group received MAMIAI,and the treatment group received XiaoErKang granule combined with MAMIAI,following-up and recording related signs disappearing time,changes of serum factors and the clinical results.Results①The fever subsided, stop vomiting and stool recovery time of treatment group were (18.24±2.13h,1.59±0.51d,3.67±0.51d),shortter than the control group with (27.05±3.09h,2.72±0.64d,5.69±0.86d),and the differences were statistically significant (P<0.05).②The hs-CRP and TNF-ɑ levels of treatment group were (4.58±0.61ng/L,8.14±0.79pg/mL),lowwer than the control group with (8.13±0.75ng/L,11.49±1.87pg/mL),and the differences were statistically significant (P<0.05).③The IL-1 and IL-6 levels of treatment group were(27.49±3.16,9.73±1.25)pg/mL,lowwer than the control group with (40.16±5.72,12.65±1.77)pg/mL,and the differences were statistically significant (P<0.05).④The treatment effect of treatment group were 91.84%,better than the control group with 73.47%,and the differences were statistically significant (P<0.05).ConclusionThe effece of XiaoErKang granule joint MAMIAI to children with diarrhea can reduce levels of inflammation,and it is effective.

Objective To explore the factors imfluencing the prognosis of patients with renal cell carcinoma after laparoscopic partial nephrectomy.Methods Clinical data from 593 renal cell carcinoma patients underwent laparoscopic partial nephrectomy in our institution from December 2010 to December 2015 were retrospectively collected..Tbere were 396 males and 197 females,aged 35 to 72 years old(mean 55.4 years).There were 181 cases of smoking history,206 cases of hypertension and 105 cases of diabetes.Before operation,98 cases of liver function were damaged and 122 cases were anemia.There were 521 cases with PLT ＜ 450 × 109/L,72 cases PLT≥ 450 × 109/L.Thc tumor of 292 cases on the left and 301 cases on the right.The Kaplan-Meier method log-rank test was used for survival analysis.Univariate analysis and factors which were significantly associated with survival in the univariate analysis were conducted into the multivariate analysis with Cox proportional hazards model.Results The operative time was 88.0-120.6 min,mean 104.3 min,the blood loss during operation was 47.2-157.8 ml,mean 102.5 ml. Pathological tumor stage revealed that 398 cases were T1 and 195 cases were T2 . Fuhrman classification revealed that 29 cases were grade Ⅰ,411 cases were grade Ⅱ,150 cases grade Ⅲ,3 cases grade Ⅳ . The median follow-up time was 36 months(ranged 6 to 99 months) . Five cases (1.3％) in the T1 patients relapsed,recurrencefree survival (RFS) rate was 98.7％;In the T2 patients,7 cases (3.6％) relapsed,1 of them had local recurrence and died of lung metastasis,and the RFS rate was 96.4％.Univariate analysis revealed that T stage,Fuhrman grade,tunor necrosis,tumor pseudocapsule,lymphovascular invasion,collection system violation,anemia,and high platelet were significantly associated with RFS of patients.Multivariate analysis found that T stage (HR =1.524,95 ％ CI 1.326-1.926,P =0.001),Fuhrman grade (HR =1.600,95 ％ CI 1.035-2.364,P =0.022),tumor necrosis (HR =2.315,95％ CI 1.523-3.624,P =0.001) were independent risk factors for the RFS of patients with renal cell carcinoma after retroperitoneal laparoscopic partial nephrectomy.Conclusion High T stage,high Fuhrman grade and tumor necrosis were independent risk factors for the RFS of patients with renal cell carcinoma after retroperitoneal laparoscopic partial nephrectomy.

Objective To compare the therapeutic effects of 1470nm diode laser vaporization prostatectomy and bipolar transurethral plasmakinetic prostatectomy (TUPKP) for treatment of benign prostatic hyperplasia (BPH).Methods From June 2015-February 2016 a total of 95 patients diagnosed with BPH were randomly divided into 2 group:52 patients were treated with TUPKP while 43 patients with 1470 diode laser.All patients were followed up with mean operative time,intra-operative blood loss,postoperative hospital stay,postoperative catheterization time,postoperative complications,international prostate symptom score (IPSS),score of life quality (QoL),maximum flow rate (Qmax),post-void residual (PVR) before and after surgery.Results Compared with data of preoperation,postoperative IPSS,QoL,Qmax,PVR in 6 months revealed significant improvement in both of two groups.IPSS of TUPKP group and 1470 group respectively dropped to 6.3 ± 2.6 and 6.7 ± 2.4.In TUPKP group,PVR reduced to 23.1 ± 20.9ml and in 1470 group decreased to 24.3 ± 19.9ml.While Qmax increased to 18.5 ± 3.1 ml/s and 18.7 ± 2.8ml/s respectively in TUPKP and 1470 group.In TUPKP group,operation time were 60.1 ± 14.9min,significantly less than that in 1470 group of 69.3 ± 12.9min (P ＜ 0.05).Compared with catheterization time in 1470 group of 33.9 ± 9.4h,the time in TUPKP group of 73.9 ± 37.6h was shorter with significant difference (P ＜ 0.05).While Curative effect of two groups of showed no significant difference (P ＞ 0.05).Conclusion The clinical curative effect of two operation methods for patients with BPH showed no significant difference.1470 group had longer operation time,while TUPKP group had less intraoperative bleeding and postoperative recovery.

Objective To observe the influence of ulinastatin (UTI) on curative effect,inflammatory response and cellular immune function in patients with acute respiratory distress syndrome (ARDS).Methods 96 patients with ARDS were randomly divided into two groups:the control group (48 cases) were treated with conventional treatment,and the UTI group (48 cases) were added with UTI injection based on the control group treatment.The changes of oxygenation index,vital signs,inflammatory factors and T lymphocyte subsets of patients in the two groups were compared before treatment and after treatment in 7 days,recorded the duration of mechanical ventilation time,ICU treatment time and mortality rate in ICU in the two groups.Results After treatment in 7 days,the vital signs (RF,HR,MAP),inflammatory factors (TNF-α,IL-6,IL-8,PCT,CRP,WBC),liver function (AST,ALT),renal function (BUN,Cr) and APACHE Ⅱ score,MODS score of UTI patients in the UTI group were lower than those in the control group (P ＜ 0.05 or P ＜ 0.01).Oxygenation index (PaO2/FiO2) and cellular immunity (CD4+,CD4+/CD8+) in the UTI group were higher than those in the control group (P ＜ 0.01).Mechanical ventilation time,ICU treatment time in the UTI group were shorter than those in the control group (all P ＜ 0.05),the mortality rate of ICU in the UTI group was lower than that in the control group (P ＜ 0.05).There was no significant difference in the incidence of adverse reactions between the two groups (P ＞ 0.05).Conclusions UTI can more effectively improve oxygen metabolism,vital signs for ARDS,liver and kidney function,reduce the APACHE Ⅱ score and MODS score,shorten the mechanical ventilation time and ICU treatment time,reduce the mortality rate of ICU.Its mechanism may relate to clearance of inflammatory factors and regulate cellular immune function of UTI.

0.05) while the level was significantly higher in the former 4 groups than in the control (P

OBJECTIVE:To establish a good method for determining the dissolution rates of diclofenac sodium suppository METHODS:The dissolution method Ⅰ and Ⅱ of ChP(2000) were compared,the first derivative spectrophotometry was used and the parameters(T50,Td,m) obtained from these samples were brought for correlation analysis RESULTS:The dissolution rate of diclofenac sodium suppository from the dissolution method Ⅰ and Ⅱ were remarkably different,and the dissolution method Ⅱ was better than Ⅰ CONCLUSION:The method established by this study is suitable for the quality control of diclofenac sodium suppository,and the percentage of dissolution of diclofenac sodium suppository in 45min more than 80% is the standard of quality control