Objective To prepare the specific monoclonal antibodies(mAb) of Mycoplasma pneumonia(MP) and to establish a colloidal gold rapid detection method of MP by using mAb .Methods The nasopharyngeal swab samples were collected from chil‐dren patients with acute respiratory tract infection ,separated and cultured and performed the MP identification ,MP antigen was pu‐rified ,mAb of MP was prepared ,then the MP colloidal gold test strip was established by using MP mAb .The throat swab sample was collected from the patients with suspected MP infection and detected by using the fluorescent quantitative PCR and colloidal gold test strip .The detection results were observed and statistically analyzed .Results MP strain was successfully isolated and inac‐tivated ,the MP antigen was purified .The mouse was immunized by using this antigen ,19 strians of mAb against MP were prepared by using the hybridoma technique .Among them ,2 strains of mAb with high titer and good specificity(MP‐5 and MP‐19) were se‐lected as the raw materials for preparing the MP colloidal gold rapid test strip .The lowest detection limit was 20 ng .The clinical samples were detected by using the MP colloidal gold rapid test strip and fluorescent quantitative PCR .The results showed that the sensitivity of MP colloidal gold rapid test strip by using this established method was 88 .2% and its specificity was 82 .6% .Conclu‐sion specific mAbs against MP is prepared and the colloidal gold rapid detection method is preliminarily established which provides the help for rapid diagnosis in the patients with MP infection .

Growth arrest special 5 (GAS5) is a common long non-coding RNA (lncRNA).Recent studies find that lncRNA GAS5 is down-regulated in various tumors,such as breast cancer,cervical cancer,lung cancer,gastrointestinal cancers and urologic cancers.GAS5 is closely related to the development,metastasis and prognosis of cancers,which becomes a new hotspot in cancer research.

Advanced hospitals and high-level hospitals are different from grass-root hospitals,since they not only treat difficult diseases but also have the obligation of scientific studies and teaching.Grass-root hospitals mainly focus on treating common diseases,and only conduct a small amount of teaching,thus resulting in the difference between grass-root hospitals and large-scale hospitals in the aspect of purchasing medical equipment.Because the money for grass-root hospitals may only be used as designated and some even comes from loan or collecting,we have to pinch pennies and make the best use of everything so as to recover the cost rapidly and bring the equipment into full play.

Objective To investigate Survivin protein and esophageal cancer with a systematic review.Methods The published studies were searched in the Cochrane Library (Issuel,2011),Pubmed and CNKI databases, and other relevant journals were also hand searched to identify all the relevant case-control trials.Then the quality of the included trials was assessed and meta-regression analysis was performed,by STATA 11.0 software.Results A total of 15 studies were recruited.As for the positive rate of Survivin expression, significant differences were tested between esophageal cancer vs.normal esophageal tissues (OR =39.98, 95 ％ CI =15.08-106.02, P ＜ 0.05).No significant difference was tested between cell differentiation G3 vs.cell differentiation G1-G2(OR =1.47,95 ％ CI =0.59-3.68,P ＞ 0.05).There were significant differences between T stagesⅢ-Ⅳ vs.T stages Ⅰ-Ⅱ (OR =2.82,95 ％ CI =1.35-5.90,P ＜ 0.05),clinic stages Ⅲ-Ⅳ vs.clinic stages Ⅰ-Ⅱ (OR =2.99, 95 ％ CI =2.03-4.41, P ＜ 0.05), lymph node metastasis vs.non-lymph node metastasis (OR =4.11,95 ％ CI =2.79-6.05,P ＜ 0.05).Moreover,Survivin expression was positively correlated with the expression of hypoxia inducible factor-1α (HIF-1α) (r =0.542,P ＜ 0.005).Conclusion Survivin expression increased malignant behaviors of esophageal cancer.There might be substantial correlations between Survivin and HIF-1α expression and esophageal cancer.

Objective To evaluate the efficacy of I-gel laryngeal mask airway (LMA) for airway management in patients undergoing radical mastectomy.Methods One hundred and twenty ASA Ⅰ or Ⅱ patients (Mallampati Ⅰ or Ⅱ),aged 25-64 yr,weighing 45-90 kg,with body mass index ＜ 30 kg/m2,scheduled for elective radical mastectomy under general anesthesia,were randomized into 2 groups (n=60 each): I-gel LMA group (group Ⅰ) and Classic LMA (group C).Anesthesia was induced with iv midazolam 0.04 mg/kg and vecuronium 0.10 mg/kg.I-gel and classic LMAs were inserted in I and C groups,respectively,after induction of anesthesia.The success rate of LMA placement at first attempt,LMA placement time,airway sealing pressure,peak airway pressure,leaks and hypoxemia,and complications (nauseas and vomiting,bucking,aspiration and blood stain on the LMAs,sore throat and hoarseness within 24 h after surgery) were recorded.Correct position of the LMAs was verified by fiberoptic bronchoscopy.Results The success rate of LMA placement were 100 ％ in both groups.The success rate of LMA placement at first attempt and fiberoptic bronchoscope scores were higher,the LMA placement time was significantly shorter,and the incidence of blood stain on the LMAs and sore throat were significantly lower in group Ⅰ than in group C (P ＜ 0.05).A transient increase in airway pressure and leaks occurred in 5 patients in group C,and the airway pressure returned to normal and no leaks developed after treatment.No transient increase in airway pressure and leaks occurred in group Ⅰ.Hypoxemia was not found in both groups.Conclusion The placement of I-gel LMA is easier than that of Classic LMA and the success rate of placement at first attempt is high,with fewer complications.I-gel LMA can be safely and effectively used for airway management in patients undergoing radical mastectomy.

Objective To observe the therapeutic effect and prognosis of patients with severe traumatic craniocerebral injury treated by Lund program combined with Angong Niuhuang pill. Methods A prospective study was conducted. Forty-nine patients with severe traumatic craniocerebral injury were divided into two groups:combined treatment group (25 cases) and control group (24 cases). All the patients in the two groups received conventional therapy. Additionally, the combined treatment group was treated by the Lund program combined with Angong Niuhuang pill, the patients after admission into the intensive care unit (ICU), indwelling of gastric tube was immediately given (the tube inserted and retained through mouth in cases with cranial base bone fracture), one pill of Angong Niuhuang was fed nasally twice a day for consecutive 15 days. Before and after treatment, the intracranial pressure (ICP), cerebral perfusion pressure (CPP), Glasgow coma scale (GCS) score, the mean flow velocity of middle cerebral artery (MCA), and the incidence of complication, clinical efficacy and prognosis in the two groups were observed. Results There were no statistically significant differences in the levels of ICP, CPP, GCS score and the average flow velocity of MCA before treatment and the first day after treatment between the two groups. Beginning from 3 days after treatment, the degrees of improvement in the above indexes in the combination treatment group became much better than those in the control group [ICP (mmHg, 1 mmHg=0.133 kPa):16.78±4.50 vs. 18.80±5.67, CPP (mmHg):71.35±1.63 vs. 58.11±1.47, GCS score:9.53±0.38 vs. 8.23±0.44, the mean blood flow velocity (cm/s):152.23±3.33 vs. 178.86±5.23, all P<0.05]. The incidences of complications such as hyperpyrexia [8.0%(2/25) vs. 41.7%(10/24)], hyperspasmia [12.0%(3/25) vs. 45.8%(11/24)] and cerebral hernia [8.0%(2/25) vs. 33.3%(8/24)] in the combined treatment group were significantly lower than those in the control group (all P < 0.05). The rates of renal failure, electrolyte imbalance and pulmonary infection in combined treatment group were also lower than those in the control group, but the differences were not statistically significant (all P>0.05). The rate of good therapeutic effect in combination treatment group was obviously higher than that of the control group [72.0% (18/25) vs. 33.3% (8/24), P < 0.05], while the mortality was markedly lowered [8.0% (2/25) vs. 33.3% (8/24), P < 0.05]. Conclusions The combination of Lund program and Angong Niuhuang pills for treatment of patients with severe traumatic craniocerebral injury can reduce the degree of increase of ICP at early stage, ameliorate cerebral edema and its development, elevate CPP and improve cerebral ischemia and hypoxia. In the aspects of controlling hyperpyrexia, decreasing limbs twitches and the incidence of hernia, elevating the quality of patients' life, reducing mortality and improving prognosis, the therapy has relatively good therapeutic effects.

Objective: To study the mechanism of Tuina in the treatment of skeletal muscle injury. Methods: Rabbits were heavily beaten at gastrocnemius muscle to make acute contusion model and then treated respectively by early Tuina and routine Tuina. The number of satellite cells of skeletal muscles was observed. Results: The number of the satellite cells continued to grow in both groups, and it began to increase significantly 3-5 days after Tuina treatment. Early Tuina treatment produces larger number of satellite cells than routine Tuina treatment.Conclusion: Early Tuina treatment is helpful to the marked recovery of skeletal muscles by increasing the number of satellite cell.

[Objective]To evaluate the effect of transverse arch reconstruction by modified Youngswick osteotomy on young and middle-aged hallux valgus. [Methods]From November 2005 to November 2008,25 female(36 feet) hallux valgus patients with the average age of 37.7years old(range 22 to 49 years old) were treated surgically.All feet had hypemobility of the first tarsometatarsal joint and collapse of the transverse arch of the foot.In the operation,the first metatarsal head was pushed laterally,downward and rotated downward.Modified McBride procedure was used on the correction for hallux valgus deformity.The X-ray films of all patients were obtained before operation,and 12 months after operation.The hallus valgus angle and intertatarsal angle were measured and analyzed on radiographs.The surgical outcome was evaluated combined with the Gu Xiang-jie's score.All pamameters were statistically analyzed.All cases were followed-up regularly.[Results]Twenty-one cases(28 feet) were followed-up for averaged 15.9 month.Hallax valgus angle reduced from 26.8??1.07? to 11.1??0.29?,intertatarsal angle reduced from 14.9??1.36? preoperatively to 8.3??1.19? postoperatively.The painful callus beneath the forefoot was alleviated or diminished obviously in all cases.Based on Gu Xiangjie'score,the subjective assessments were given as excellent in 16 feet,good in 7 feet,and fair in 5 feet.The good to excellent result was 82.1%.[Conclusion]For the hallux valgus with hypermobility of the first tarsometatarsal joint and transversal arch collapse,modified Youngswick surgery can recover the transverse arch,correct first metatarsal adduct abnormality,and regain the weight-bearing function of the first metatarsal head.Modified Youngswick surgery is an ideal choice for young and middle-aged hallux valgus because of less complications and reliable surgery outcomes.

Objective To understand whether Imarcaptoacetate dioctyltin(IMA) is a environmental endocrine disruptor or not. Methods The new born Wistar rats were randomly divided into six groups including positive control group, negative control group and four experimental groups. The rats in the experimental groups were given IMA at different concentrations (0.100 0, 0.010 0, 0.001 0, 0.000 1 ?g/10 g) 1/1 000 000 by hypodermic injection, the rats in the negative control group were treated with corn oil and those in the positive control group were treated with dibutyltin dilaurate(DBTD) at concentration of 0.001 ?g/10 g bw. The rats were injected one time each day for five consecutive days. After one and half months, the levels of T, LH and FSH in the blood, the weight of the testicles, ovaries and the sexual organ exponent were determined. The pathological examination on some samples was conducted. Results In both of female and male, the levels of T in IMA treated rats were significantly increased and showed an doge-effect relationship (P0.05). The exponents of the testicles were different among groups (P

Objective To understand the situation of rebuilding sanitary latrine in the rural areas in Qingdao and to evaluate the effect of sanitary latrine improvement, explore the new measure of sanitary latrine improvement in the rural areas. Methods In 2005, the typical method of investigation was used to choose 365 farmers from 8 rebuilding sanitary latrine villages among three counties in Qingdao, and to conduct on-site survey and health questionnaires on the construction of household latrines and excreta treatment, sanitation and related knowledge. Hygienic evaluation was made in accordance with hygienic standard for rural household latrine. Results 100% of the houses had the household latrines, 80.0% were the sanitary latrines, dejecta treatment rate was 78.36%. Conclusion All evaluation indexes reach the high level, the effect of household latrine improvement in the investigated rural areas is satisfactory.