MicroRNAs (miRNAs) are a class of small,non-coding,single-stand RNA molecules which are composed of 21-25 ribonucleotides.They regulate gene expression at post-transcription and participate in many important biological processes including cell growth,apoptosis,viral infection and cancer development.At present,the studies on miRNAs in cervical lesion include miRNAs expression characteristic of cervical cancer and precancerous lesion,the association with HPV infection and prognosis of cervical lesion,cervical cancer development,diagnosis and therapy.

Objective To observe the clinical feasibility of the acceptable noise level (ANL) test ,analyze the effect of different test instructions to the result of Mandarin acceptable noise level test .Methods Twelve young peo-ple (24 ears) with normal pure-tone test and acoustic immittance test (threshold≤15 dB HL) were included in this study .They were randomly divided into two groups with 12 ears in each group .Group A received the same test in-struction firstly and then received different instructions depended on the different translation of English instruction by tester .Group B received the reverse test order .The acceptable noise level(ANL) ,most comfortable level(MCL) and back groud noise level(BNL) were analyzed .Results The average value of ANL was 7 .5 ± 6 .61 and 8 .29 ± 6 .54 dB SPL ;the average value of most comfortable level (MCL) was 83 .58 ± 8 .57 and 85 .41 ± 8 .89 dB SPL ;the average value of background noise level (BNL) were 78 .92 ± 10 .56 and 77 .13 ± 7 .91 dB SPL respectivoly .The difference of MCL ,BNL ,ANL in these two groups were not statistically significant (P>0 .05) .Test method 1 re-sulted in great individual differences in ANL ,which it ranged from -6 .4 to 20 .12 dB S/N .While ANL from test method 2 ranged from 2 .50~20 .12 dB S/N .Conclusion Different test instruction do not correlated to the results of acceptable noise level ,testers can translate the English instruction by different ways so that it is convenient to the application and clinical research of ANL test .

Objective: To improve the micro-manipulation of mouse fertilized eggs,promoting its survival rate and productivity in pseudo-pregnant mice. Methods: DNA fragments of definite concentration and size were used for micro-manipulation with conventional and improved methods, then their survival rate of manupulated eggs and productivity of pseudo-pregnant mice were analyzed and compared. Results: With improved methods, the survival rate of eggs increased from 60.3% to 79.5%, the productivity of pseudo-pregnant mice and offspring increased from 35.7% to 56.8% and 10.1% to 14.1% respectively. Conclusion: The improved methods of micro-manipulation can be used in transgenic mouse production.

Objective: To study the effect of chronic stress on spatial learning and memory and nitric oxide (NO) in hippocampus of the rats Method: Chronic stress model was established by electric-foot accompanied with noise Morris watermaze was used to observe spatial learning and memory abilities, contents of NO and activities of NOS in hippocampus were measured during the test Result: The spatial learning and memory of chronic stress rats were significantly decreased in Morris watermaze Contents of NO (3 87?0 47 nmol/mgpro) and NOS activity (102 64?13 33 pmol/mgpro/min) in hippocampus of the stress rats were significantly higher than that of normal rats (2 76?0 43 nmol/mgpro, 78 25?10 67 pmol/mgpro/min respectively) Conclusion: Chronic stress impairs spatial learning and memory abilities of rats, which may be related to the increase of NO in their hippocampus

A brief description of the medical treatment assurance service provided by the hospital for major international events from 2001 to 2007. The paper rounded up the practices and experiences of the hospital gained such services in a variety of international events, including the development of a detailed operative plan for medical treatment assurance, selection and preparation for medical workers, development and updating of training programs, determination of the operability of the plan and successful launch of the assurance services. It holds that provision of such services significantly upgrades the general competence of the hospital management, and its capabilities in management, emergency response, rescue and inter-department coordination.

Such measures as improved internal management and process, and utilization of contemporary management theories and scientific means, efficiently streamlined resourcea of all kinds at the outpatient clinic. Service quality and level have been significantly raised for appointment practice at the outpatient clinic, as evidenced by a growing percentage of appointments scheduled. On this basis,problems found in the existing appointment system were analyzed along with improvement suggestions.

Objective To determine the effect of laparoscopic abdominal wall components separation procedure on a porcine model of intra-abdominal hypertension (IAH). Methods IAH to 25 nun Hg was created by insufllating carbon dioxide into abdominal cavity of eight anesthetized pigs. Bilateral subcutaneous tunnels above the plane of the abdominal musculature were developed. Dissection of the external oblique insertion and development of the plane between external and internal oblique muscles was performed using ultrasonic scalpel monitored by laparoscopy. Changes in intra-abdominal pressure (IAP), waistline (WL), mean arterial pressure (MAP), heart rate (HR), central venous pressure (CVP) were recorded. Results Following intra-abdominal hypertension, WL, MAP, HR and CVP changed significantly. After laparoscopic abdominal wall components separation, there was a significant decrease in IAP from 25 mm Hg to ( 16. 0 ± 1.5 ) mm Hg ( P < 0. 01 ). Also, this procedure increased WL by (5.6 ± 2. 0) cm [ from (65.3 ± 2. 5 ) cm to (88 ± 14) cm; P < 0. 01 ]. Due to abdominal depression, the MAP was significantly increased from to (88 ± 14) mm Hg to ( 100 ± 12) mm Hg ( P <0. 01 ). Moreover, there were significant improvements in circulation status including HR from (115 ± 9) beat per minute (bpm) to (97 ± 7) bpm (P<0.01), CVP from (10.8±2.2) cm H2O to (7.3 ± 1.8) cm H2O (P<0.01), etc. Conclusions IAH results in changes of hemedynnmies. Laparoscopic abdominal wall components separation significantly decreases IAH improving porcine hemodynamics.

Objective To determine whether pancreatic ductal preservation(pancreatic ductal injection of sparing TIUW solution at the time of pancreas procurement)can improve islet yield and function after cold preservation of pancreas.Methods Pancreases were classified into five groups:fresh pancreases(group1 ,n= 10);preserved for 6 hr in TIUW solution without and with pancreatic ductal preservation by TIUW solution(group2, n=10 and group3, n = 10);preserved for 24 hr in TIUW solution without and with pancreatic ductal preservation by TIUW solution(group4 ,n= 10 and group5,n= 10).Dithizon(DTZ)staining was used to identify islet morphous and yield, trypan blue uptake(TBU)was used to assess the nonviability of islets, the insulin secretory response to glucose was used to evaluate islet function in vitro, islet transplantation was used to determine islet function in vivo.Results Islet yields per pancreas after purification in group 1 to 5 were 590± 127, 272 ± 50,454 ± 65, 253± 56, 447 ± 66(islet equivalent ± SD), respectively.Percentage of nonviable islets in grouplto5were(5.7±4.2)%,(18.3±6.5)%,(11.7±4.2)%,(26.3±5.6)%,(15.0±5.3)%.Stimulation index in group 1 to 5 were 7.32±2.32, 4.81±1.17, 7.56±2.44, 2.88±1.00, 5.71±1.90.Cure rates in group 1 to 5 were 100%, 0%, 100%, 0%, 70%.In this study, the differences were significant between control groups and experimental groups(P＜0.05, group 2 vs.group3 and group4 vs.group5).Conclusion The pancreatic ductal preservation can improve islet yield and function.

Objective To discuss effective nursing measures for patients with congenital hypospadia with the application of urethral stent.Methods 142 patients with congenital hypospadia were selected and used soft stent during and after operation.The urethral orifice was kept smooth after operation and the stent was removed until 2 weeks after operation.Results Two patients complicated with urethral fistula,one was because of the poor blood supply of the skin flap and the other because of the edema of the skin flap and the remove of stent.Other patients received good treatment effect. Conclusion Application of urethral stent in patients with congenital hypospadia could reduce the incidence of infection and increase the success rate of operation.

Objective To analyse the efficiency of postural control after 24h sleep deprivation.Methods To observe the change of the static posturography of 16 healthy male after 24h sleep deprivation.Results After 24h sleep deprivation,in both eyes-open condition,whole path length(0h:564.40±42.07,24h:535.23±33.28),unit-path-length(0h:6.96±3.20,24h:5.49±2.65),deflection average center displacement X (0h:7.01±2.79,24h:5.06±3.73)significantly decreased,but standard deviation Y(0h:3.17±0.83,24h:3.98±1.34)significantly increased.In both eyes-closed condition,whole path length(0h:645.70±80.05,24h:587.27±64.87)significantly decreased.Conclusion After 24h sleep deprivation,there are less spontaneous movements of body and lower efficiency of postural control.