According to TCM theory of the wholistic concept and the principles of syndrome differentiation and treatment, the characteristics of the pathogenesis of aplastic anemia (AA) were summarized and corresponding treatment was put forward on the basis of clinical observation and the study of literature. In the pathogenesis of AA, the deficiency of spleen and kidney is the primary aspect, fire attacking blood vessels is the secondary aspect and there is change because of blood stasis. In treatment, the method of invigorating spleen and tonifying kidney is adopted in order to get at the root, and releasing fire to stop bleeding is adopted in order to bring about a temporary solution, and activating blood circulation to dissipate blood stasis is adopted in order to produce blood. The outline should be caught hold of that consumption is primary aspect and fire is secondary aspect and there is change because of blood stasis in order to obtain satisfactory curative effect.

With the progress of minimally invasive surgical techniques,the vast majority of general surgery can be safely carried out laparoscopically.But the development of the pancreas laparoscopic surgery is lagging behind.Initially laparoscopy was used only as a diagnostic and staging tool.Nevertheless with more and more successful launch of laparoscopic surgery.Surgeons devoted a great deal of energy to this field in order to benefit more patients.This review focused on the literature of laparoscopic pancreatic surgery in resent years,although the majority of literature suggests that the minimally invasive pancreatic surgery has advantages of rapid recovery,low complication rate compared to traditional open surgery.However,most studies are limited to a small sample and a single institution results.The promotion of this emerging technology still needs more rigorous evidence based medicine.

Objective To observe the apoptosis and expression of Toll-like receptor (TLR)-4's mRNA in ischemia-reperfusion injured rat renal tubular epithelia cells (NRK-52E) in vitro, and effect of Cordyceps Sinensis (CS) extractant.Methods Cultured NRK-52E cells were divided into a control group, a model group, and a CS preincubated group. All first removed media and incubated with antimycin A for 1 h, and then recovered the media to simulate the ischemia-reperfusion injury in vitro. We detected the apoptosis ratio of cells by flow cytometer and the mRNA expression of TLR-4, Bax, Bcl-2 gene by reverse transcription-polymerase chain reaction at different time points.Results In ischemia-reperfusion injured NRK-52E, the apoptosis ratio rose as time passed (P＜0.05). We also observed increased mRNA expression of TLR-4, Bax (P＜0.05) and deceased expression of Bcl-2 (P＜0.05). Compared with the model grpup, the CS preincubated NRK-52E cells showed apparent tolerance to ischemia-reperfusion injury, which manifestated lower apoptosis ratio (P＜0.05), decreased expression of mRNA of TLR-4, Bax and increased expression of Bcl-2 (All Ps＜0.05). Conclusion The number of apoptosis cells and the expression of TLR-4 mRNA inceased with ischemia-reperfusion injury of NRK-52E in vitro. CS can prevent the NRK-52E cells from ischemia-reperfusion injury by downregulating TLR-4 gene.

Objective To study the correlation of heart rate variability(HRV) and autonomic nervous function in patients with anxiety neurosis, and provide evidence for prevention and treatment of anxiety disorder. Methods 90 patients with generalized anxiety disorder(GAD) and panic disorder(PD) and 90 healthy persons were tested by psychological questionnaire,the anxiety and depression were assessed by HAMA and HAMD. The average heart rate and HRV index were monitored by 24-hour ambulatory ECG. Results The average heart rate of the anxiety group was higher than the control group, compared with the control group the difference was significant ( P < 0. 05 ); The HRV parameters of the anxiety group were lower than the control group, compared with the control group the difference was significant( P < 0. 05 ) ;The HRV parameters of the GAD group were lower than the control group, the HRV parameters of the PD group were lower than the GAD group, the difference was significant ( P < 0. 05 ). Conclusion The patients with anxiety neurosis had high sympathetic nerve activity function and autonomic dysfunction, the PD patients had higher sympathetic nerve activity function and autonomic dysfunction than the GAD patients.

Objective To investigate the status of post traumatic growth and role reinforcement in elder patients with cerebral infarction accepting the treatment of rehabilitation, and to explore the effect of role reinforcement on post traumatic growth. Methods Totally 303 patients with cerebral infarction accepting the treatment of rehabilitation were investigated by the Post Traumatic Growth Rating Scale and the Role Reinforcement Scale. Results The post traumatic growth and role reinforcement scores of the patients were (61.50 ± 7.19), (63.51 ± 9.28) points. The scores of each dimension of post traumatic growth were significantly positively correlated with the role reinforcement (r =-0.794--0.123, P < 0.05 or 0.01). Stepwise regression analysis showed that the influence factors on the post traumatic growth of the patients were the cultural level, age and each dimension of role reinforcement (P < 0.05). Conclusions For the patients with cerebral infarction accepting the treatment of rehabilitation, the level of post traumatic growth is in a moderate level, and the role reinforcement is in a strong level, and role reinforcement is the influencing factor of post traumatic growth.

Objective To survey the point prevalence of healthcare-associated infection(HAI) in 13 tertiary hospitals in Dongguan,understand the occurrence of HAI in tertiary hospitals,so as to provide guidance for the prevention and control of HAI.Methods Through bedside investigation and medical record reviewing,HAI among inpatients in 13 tertiary hospitals in Dongguan at 0:00-24:00 of September 24,2014 were investigated.Results A total of 11 344 patients were investigated,379 patients developed 404 times of HAI,HAI rate was 3.34％,HAI case rate was 3.56％.Incidence of HAI in the intensive care unit was highest (12.82％).The main infection site was respiratory tract(n =173,42.48％).The usage rate of antimicrobial agents was 30.50％,most were for therapeutic use (including therapeutic+ prophylaxis) (n =2 545,73.55 ％)and single use (n =2 689,77.72％),pathogenic microorganism detection of specimens from patients receiving therapeutic antimicrobial use was 53.79％.A total of 299 strains of pathogenic orgamisms were detected from infected patients,the major were Escherichia coli (n =56,18.73 ％),Pseudomonas aeruginosa (n =54,18.06 ％),and Klebsiella pneumoniae (n =35,11.71 ％),92 strains of multidrug resistant organisms(MDROs) were isolated,accounting for 30.77％ of total isolated pathogens.Conclusion The basic characteristics of HAI in tertiary hospitals in this city were preliminarily investigated,identification of high risk departments,high risk population,and high risk links should be strengthened,HAI prevention and control measures should be implemented.

Mammals have three genes with homology to the hh gene.These comprise Sonic hedgehog(Shh),Indian hedgehog(Ihh),and Desert hedgehog(Dhh).Shh has been shown to play a crucial role in embryogenesis and the development of ectoderm.Shh has the great relationship with the forming all the system.Shh-Patched-Smoothened signal pathway will lead the embryogenesis in the normal way.Otherwise,the abnormal embryogenesis,malformation,and tumor will be happened.

AIM: To investigate the gene expression of proteins handling the calcium homeostasis in diabetic rat heart. METHODS: Diabetes was induced in male Sprague-Dawley rats (180-220)g by administering a single tail-vein injection (40mg/kg ) of alloxan, and the control group was injected with normal saline. At the end of four, six weeks after the induction of diabetes, the animals were killed for assessment of the ventricular gene expression of proteins handling the calcium homeostasis which was measured by reverse transcription-polymerase chain reaction and nomalized to the mRNAs level of actin. RESULTS: At the end of 4 and 6 weeks, the weight of body and the heart in the diabetic group is lower than that in control rats, heart to body weight of diabetic rats is higher than control. No changes in mRNAs level of sarcoplasmic reticulum calcium ATPase, phospholamban, ryanodine receptor type 2, 1,4,5-trisphosphate inositol receptor type 2 were observed 4 weeks after alloxan injection. Expression of phospholamban mRNAs was significantly increased, but ryanodine receptor type 2, 1,4,5-trisphosphate inositol receptor type 2 mRNAs were significantly decreased than that in the age-matched controls. In contrast, expression of mRNAs coding sarcoplasmic reticulum calcium ATPase was not affected 6 weeks after alloxan injection. CONCLUSION: Gene expression of phospholamban is increased while ryanodine receptor type 2, 1,4,5-trisphosphate inositol receptor type 2 are decreased in the diabetic rat heart. [

OBJECTIVE To investigate Epstein-Barr virus (EBV) infection and clinical symptoms among hospitalized children by detection of EBV in their throat swabs. METHODS During from Oct 2004 to Apr 2006, EBV-DNA was detected from throat swabs of inpatients in pediatric ward by the technology of fluorescence quantitative polymerase chain reaction. RESULTS Totally 3429 cases were detected, 738 cases were positive. The positive rate was 21.5%. In different age groups, the rate of EBV infection was 18.8% for ≤3 years old, 26.5% for 4-7 years old, 29.0% for 8-11 years old, and 31.7% for 12-15 years old, respectively. The rate of EBV infection increased significantly with age increasing (P

Objective In order to optimize patient's medical treatment processes, and to improve the working efficiency of health care professionals and the quality of medical services. Methods The design and application of the electronic prescription module were introduced, and its advantages and existing problems were discussed. Results The electronic prescription module were applied generally. Orthodox patient's medical treatment processes were changed and the prescription management was normalized. Conclusion The electronic prescription module is one of the core components of the workstation system for the out-patient physicians. It optimizes the patient's medical treatment processes and improves the efficiency and the quality of medical services.