In recent years, more and more researchers focus on the selectivity of RP-HPLC columns. Among these columns, C18 column is the most commonly used the column. Yet even the same filler can not give us the same separation effect with the columns of different brands and different types, therefore, it is important to choose the suitable HPLC column for carrying out an experiment. After study in recent years, some column classification, selection methods (e.g.: column parameter method, F value method) have been achieved and contributed to the finding of suitable columns and reducing the blindness of column selection. This paper summarizes the advantages of these methods and their limitations in the application process, and gives some advices and expectations to pharmaceutical analysis works based on the works of other researchers.

Objective To evaluate laparoscopy assisted radio frequency ablation (LRFA) and percutaneous radiofrequency ablation ( PRFA ) for the treatment of hepatocellular carcinoma ( HCC ).Methods A total of 525 HCC patients were enrolled,including LRFA group of 78 cases with 106 tumor nodules,and PRFA group of 447 cases with 565 tumor nodules.Results ( 1 ) In LRFA group complete ablation rate was 97.17％ (103/106); in LRFA group complete ablation rate was 93.09％ (526/565)(x2 =2.523,P =0.112).(2)The 1,3,5 year overall survival rate was 96.15％,55.12％ and 38.46％in LRFA group and 93.73％,48.54％ and 31.54％ in PRFA group respectively ( x2 =0.699,1.151,1.447 ; P =0.403,0.283,0.229 ).The 1,3,5 year disease-free survival rate was 94.87％,43.58％ and 28.21％ in LRFA group and 93.73％,48.54％ and 31.54％ in PRFA group respectively (x2 =0.915,0.303,0.174; P=0.339,0.582,0.676).The average disease-free survival time was 22.25 months in LRFA group and 21.53 months in PRFA group respectively.(3)The serious complications was 0％ (0/78)in LRFA group and 1.34％ ( 6/447 ) in PRFA group respectively.( 4 ) Recurrence rate was 23.07％(18/78) in LRFA group,and 34.89％ (156/447) in PRFA group ( x2 =4.189,P =0.041 ).Conclusions The therapeutical effect of LRFA equals that of PRFA,while enjoying lower recurrence rate,no serious complications and higher treatment safety.

The emulsifiers of the ferula oil was shown to suppress markedly the swelling of the hind paw induced by injection of carrageenin and to suppress the increase of vascular permeability induced by intrad-ermal HA or 5-HT in rats. It also showed significant suppressive effects on oedema of rats hind paw induced by CFA.Experimental results indicated that it has inhibiting effect on DTH by SRBC in mice and suppressing on lymphocytes transformation induced by PHA.

This article analyses and describes the influencing mechanism of hospital alliance on quality of care from six aspects:product and technology,organizational structure,psychosocial climate, management,organizational culture,and competition in medical service market.In order to ensure and improve the medical quality,this study discusses that hospital alliance can not only ally in the form but also optimize the internal structure,strengthen the integration of organizational culture of the institutions, gradually form a social psychological climate of“Quality First”.In addition,the government′s regulation on the medical service market is also indispensable.

The paper expounds the humane landscape condition and resource of Zhejiang TCM ,puts emphasis on the evaluation of hu-mane connotation of Hangzhou Huqing Yutang, Yiwu Danxi Lingyuan and Lanxi Zhuge Village Dajingtang pharmaceutical mu-eums, also proposes detailed plan of Hangzhou TCM humane landscape construction.

This study investigated the inhibitory effect of grape seed proanthocyanidin extract (GSPE) on selenite-induced cataract formation in rats and the possible mechanism. Eighty 8-day-old Sprague-Dawley rats were divided randomly into 5 groups: control group, model group, three GSPE groups (low dose, medium dose and high dose). Control group received subcutaneous injection of physiological saline. Model group was given subcutaneous injection of sodium selenite (20 μmol/kg body weight) on the postpartum day 10, and once every other day for consecutive three times thereafter. GSPE treated groups were respectively administered GSPE at doses of 50, 100, and 200 mg/kg body weight intragastrically 2 days prior to the selenite injection (that was, on the postpartum day 8), and once daily for fourteen consecutive days thereafter. The opacity of lenses was observed, graded and photographed under the slit lamp microscopy and the maximal diameter of the nuclear cataract plaques was measured. The lenses were analyzed for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), calcium (Ca(2+)), nitric oxide (NO) and anti-hydroxyl radical ability (anti-OH(-)). The histomorphology of lenses was observed with HE staining under a light microscope. The levels of calpainII, and iNOS protein and mRNA expression in lenses were detected by using immunohistochemistry and real-time quantitative RT-PCR. The results showed subcutaneous injection of sodium selenite led to severe nuclear cataract in model group, and the achievement ratio of model group was 100%. As compared with model group, the degree of lenses opacity and the maximal diameter of nuclear cataract plaques were significantly reduced in GSPE-treated groups. Moreover, we observed selenite treatment caused a significant decrease in the activities of antioxidative enzymes (SOD, CAT, GSH-PX) and anti-OH(-) ability, accompanied by a significant increase in the levels of MDA, NO, Ca(2+) as well as iNOS, and calpainII protein and mRNA expression. Administration of GSPE could dose-dependently preserve the activities of these antioxidative enzymes and anti-OH(-) ability, accompanied by a significant reduction in the levels of MDA, NO, Ca(2+) as well as iNOS, and calpainII protein and mRNA expression. These results suggested that GSPE markedly prevented selenite-induced cataract formation probably by suppressing the generation of lipid peroxidation and free radicals as well as the activation of iNOS, and calpainII in the lenses.

目的观察应用高氧液治疗急性脑梗死的临床疗效。方法将96例急性脑梗死患者分为高氧液组(48例)和对照组(48例),两组均在常规药物治疗的基础上配合康复训练,高氧液组则在静脉点滴前将待静点的液体500 ml制备成高氧液,再加入相应药物。两组患者分别于治疗前及治疗14 d后根据改良爱丁堡-斯堪的纳维亚量表(MESSS)和Barthel指数进行评定。结果两组患者治疗后MESSS评分和Barthel指数均较治疗前有改善,高氧液组明显优于对照组(P<0.01)。结论高氧液治疗可促进急性脑梗死患者神经功能的恢复,提高患者的日常生活活动能力。

Objective To systematically review the current research status of negative pressure wound therapy in China based on method of evidence-based medicine (EBM). Methods A systematic retrieval was undertaken on literatures related to negative pressure wound therapy in China. Evidence grading of EBM and quality rating system of preventive service task force were used for evaluation of the results. Results We finally retrieved five randomized controlled trials (RCT), of which only one RCT was classified as good quality and high grade of evidence, whereas the other four were classified as poor quality and low grade of evidence. Conclusions With relatively wide indications, there still lack sufficient evidences to witness the clinical efficiency of NPWT in China. in the future, we should further researches on NPWT including emphasizing on novelty of research topics and intactness and usefulness of experimental design and standardize clinical application of such technique including standardization of treatment procedures. In the meantime, high quality research papers are needed to provide certain guidance for clinical therapy.

Objective To investigate the allngeneic bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) transplantation on cardiac rehabilitation and cardiac function after acute myocardial infarction (AMI). Method Acute myocardial infarction models were made by ligating the left anterior descending coronary artery of rats. A total of 90 rats were randomly divided into the cell transplantation group and control group, 45 rars in each group. Rats in the cell transplantation group were subdivided into 3 groups according to the administration time of the allogeneic bone marrow mesenchymal stem cells (2 weeks, 4 weeks and 12 weeks), and the control group was subdivided the same as in the cell transplantation group. The success criteria included the anterior my-ocardial wall darked and the Sr-segment elevation. The rat femur bone marrow cells were separated using centrifu-gal method, mononuclear cells were isolated by lymphocyte fluid, and the third generation of mesenchymal stem cells were obtained by washing, culture, passage and marked by 4, 6 diacetyl-2-phenyl Indole (DAPI). The ani-mals' chest was opened again a week later, the stem cell suspension was injected into the border zone of infarct area, and the control group was injected with medium. Pre-operative,2 weeks, 4 weeks and 12 weeks after trans-plantation, cardiac function was tested by ultrasonic, myocardial biopsy was observed by HE conventional staining, cardiac stem cells division were observed by immunofluorescence. All data was analyzed by software SPSS 10.0, Independent-Samples T test and Chi-Square test was used, and P≤0.05 was considered as statistically signif-icant. Results The markered hone marrow mesenchymal stem cells were left in the myocardial cells and differen-tiated to eardiomyocyte-like cells. After 12 weeks, the left ventricular internal diameter of rats in cell transplanta-tion group was about (0.58±0.09) mm(P<0.05). Thickness of myocardial infarction zone was (0.25±0.01) mm (P<0.05),ejection fraction was (67.52±0.61) (P<0.05) and left ventricular fractional shortening was (39.86±3.00) (P<0.05). In the transplantation group, the DAPI marked nucleus presented as blue, and the cytoplasm of myocardial cell presented as green. HE staining showed that, in the cell transplantation group, there was less scar tissue and a large proportion of nuclear, similar to the fetal or newhom myocardium cells. Conclu-sions The homogeneus mesenchymal stem cell could repair myocardial infarction and improve cardiac function af-ter infarction.

Objective To explore the feasibility of using Zeocin resistance as a new positive selection marker for the genetic manipulation of Candida albicans (C.albicans).Methods The susceptibility of C.albicans strain CAI4 to Zeocin was determined using a broth microdilution method in pH range from 6.0 to 8.0.The ZeoR expression cassette was amplified from plasmid pGAPZ-α-A by polymerase chain reaction (PCR).An integrated vector used to knockout AAF1 gene was constructed by gene splicing.Then the C.albicans CAI4 was transformed with this integrated plasmid containing the ZeoR expression cassette using lithium chloride method and the target AAF1 gene was replaced.The recombinant C.albicans was screened and the genome was extracted and amplified by PCR with two pairs of primers,then confirmed using genetic method.Results The C.albicans CAI4 was sensitive to Zeocin in pH range from 6.0 to 8.0.And the concentration of Zeoein(100 mg/L) at pH 7.0 was used to select recombined C.albicans.The recombined integrated vector was confirmed by DNA sequencing.The ZeoR expression cassette was detected using PCR method in recombined C.albicans.The AAF1 gene was replaced by ZeoR gene.Conclusion A new vector for targeted gene disruption in C.albicans is successfully constructed.