BACKGROUND:Stem cel transplantation is a promising treatment for advanced liver diseases, and adipose-derived mesenchymal stem cels are a hot topic folowing bone marrow mesenchymal stem cels. OBJECTIVE:To explore the therapeutic effect of adipose-derived mesenchymal stem cels transplantationvia the hepatic artery on advanced liver diseases in rats. METHODS:Forty-five rats were randomized into three groups, 15 rats in each group: control group, model group and transplantation group. Rat models of liver cirrhosis were made in the latter two groups through subcutaneous injection of carbon tetrachloride. Then, 1 mL of CFSE-labeled adipose-derived mesenchymal stem cels was infusedvia the hepatic artery in the transplantation group, and the same volume of normal saline was infused in the model group. Control group had no treatment. Pathological changes, liver function and degree of hepatic fibrosis were observed in the three groups at 4 weeks after treatment. RESULTS AND CONCLUSION:After transplantation, green fluorescence-labeled adipose-derived mesenchymal stem cels were seen in the liver of rats. Hematoxylin-eosin staining and Masson staining showed unclear hepatic lobule structure in the model group with the formation of false lobules, cel cloudy sweling and loose, some degeneration and necrosis, and inflammatory cel infiltration; in the control group, there was nothing abnormal in the liver tissues of rats in the control group; in the transplantation group, the pathological changes of the rat liver were better than those in the model group, but worse than those in the control group. Compared with the model group, the level of serum albumin was higher in the control and transplantation group (P < 0.05), and the levels of bilirubin, aminotransferase and type IV colagen were lower in the control and transplantation group (P < 0.05). Thus, it can be seen that adipose-derived mesenchymal stem cel transplantation can improve liver function and reduce liver fibrosis in cirrhotic rats.

Objective To investigate the pathogenesis of the susceptibility to cardiovascular diseases in patients with psoriasis. Methods The composition of serum free fatty acid(FFA) were measured by gaschromatography in 33 patients with psoriasis vulgaris and normal individuals. Results ① Along with the increase of severity of psoriasis the levels of poly unsaturated fatty acid(PUFA) increased, mono unsaturated fatty acid (MUFA) and saturated fatty acid (SFA) decreased and PUFA/SFA ratio increased. ② The level of linoleic acid (LA) was significantly higher in the patients of progressive stage and with extensive skin lesions than that in the normal group and/or stationary stage, and the level of oleic acid decreased. ③ The changes of the composition of FFA in patients with psoriasis were diffrent from the majority of the reports studied in the patients with cardiovascular disease. Discussion and Conclusion In the severe stage of psoriasis, the metabolism of fat in the liver is accelerated; the increase of LA, which supplies enough precursor for arachidonic acid metabolism, might be related to the loss of granular layer of epidermis. The changes of FFA induced by psoriasis might be a link causing cardiovascular disease but it should be studied further.

OBJECTIVE:To study the bioequivalence of 2 kinds of Losartan potassium tablets in healthy volunteers. METHODS: A single oral dose of test tablet 100 mg and reference tablet 100 mg were given to 20 healthy volunteers in randomized crossover study. The plasma concentrations of losartan and its metabolite EXP3174 were determined by LC-MS. The pharmacokinetic parameters were calculated and bioequivalence of 2 kinds of tablets was evaluated. RESULTS: Main pharmacokinetic parameters of Losartan of test tablets vs. reference tablets were as follows: Cmax(534.230?238.642) ng?mL-1 vs.(520.020?226.800) ng?mL-1; tmax(1.401?0.946)h vs.(1.334?0.750)h; AUC0～36(871.177?232.315) ng?h?mL-1 vs. (773.030?252.209) ng?h?mL-1; pharmacokinetic parameters of metabolite EXP3174 of test tablets vs. reference tablets were as follows: Cmax (1 294.000?387.815) ng?mL-1 vs.(1 140.900?317.615)ng?mL-1;tmax(2.667?1.144)h vs.(2.734?1.162)h;AUC0～36(6 267.905?1 350.300)ng?h?mL-1 vs.(5 719.411?1 127.725) ng?h?mL-1; AUC0～∞(6 316.605?1 343.048)ng?h?mL-1 vs. (5 755.335?1 138.358) ng?h?mL-1. The relative bioavailability of test tablet was (116.6?24.3)%. CONCLUSION: 2 kinds of Losartan potassium tablets are bioequivalent.

Objective To investigate the role of chemokine ligand 21 (CCL21) in the spinal cord in tibia bone cancer pain (BCP) in rats.Methods Forty adult female SD rats weighing 160-180 g were randomly divided into 5 groups (n=8 each):sham operation group (group Ⅰ ); sham operation + CCL21 neutralizing antibody group (groupⅡ); BCP group (group [); BCP + PBS group (group Ⅳ); BCP + control IgG group (groupⅤ)and BCP + CCL21 neutralizing antibody group (group Ⅵ).BCP was induced by inoculating Walker-256 mammary gland carcinoma cells into the rat tibia medullary cavity in groups Ⅲ-Ⅵ.PBS 15 μl,IgG 10 μg and CCL21 neutralizing antibody 10 μg were injected intrathecally (IT) at 14 days after intra-tibial injection of Walker-256 mammary gland cancer cells in groups Ⅳ- Ⅵ respectively.Mechanical withdrawal threshold to yon Frey filament stimulation (MWT) was measured at 1 day before (To,baseline) ; 7 and 14 d after Walker-256 cell injection (T1,T2)and at 0.5,1,2,4,8,12,24 and 48 h after intrathecal injection (T3-10 ).Results Intra-tibial injection of Walker-256 mammary gland cancer cells significantly decreased MWT as compared with the baseline values in administration of CCL21 neutralizing antibody at T5-8 as compared with MWT before intrathecal administration at T2 in group Ⅵ.MWT was significantly lower in groups Ⅲ- Ⅳ than in groups Ⅰ and Ⅱ.MWT was significantly higher at T5-8 in group Ⅵ than in groups Ⅲ - Ⅴ.Conclu]sion CCL21 in the spinal cord is involved in the maintenance of tibia BCP in rats.

ObjectiveTo explore the role of CXCL16/CXCR6 axis on the metastasis of human lung cancer.MethodsImmunohistochemistry and immunocytochemistry analysis were performed to detect the expression of CXCL16/CXCR6 in human lung cancer samples as well as A549,95D and H292 cell lines,respectively.The effects of CXCL16 on the viability and invasiveness of the three lung cancer cell lines were examined by MTT and in vitro invasion assay,respectively.ResultsHuman native lung cancer cells co-expressed CXCR6 and CXCL16 protein.Compared to the normal lung tissues,there was a stronger specific staining for both CXC16 and CXCR6 protein in the lung cancer tissues.Three kinds of lung cancer cell lines,including A549,95D and H292,all expressed CXCL16 and CXCR6 protein.Furthermore,human recombinant CXCL16 significantly promoted the viability and invasiveness of A549,95D and H292 cells.The stimulated action of CXCL16 on lung cancer cell lines could be effectively blocked by CXCL16 neutralizing antibody.Conclusion CXCL16 and CXCR6 protein are co-expressed in human native lung cancer.CXCL16 is able to promote the viability and invasiveness of lung cancer cell lines,which might be the molecular mechanisms on the metastasis of lung cancer.

Objective To explore the safety and effectiveness of transurethral resection of the prostate (TURP) for large benign prostatic hyperplasia.Methods A total of 56 cases of patients with large benign prostatic hyperplasia were analyzed retrospectively.Results The weight of prostate tissue resected with TURP was 60.2 g (48-122 g).The resection rate was 60％,operation time 113.4 min (70-180 min).The lower urinary tract symptoms (LUTS) after TURP was improved significantly.The international prostate symptom scores (IPSS) of 38 cases were decreased from (25.6±1.5) before operation to (6.0± 2.0) after operation.The maximum urine flow rate were increased from (5.0±1.9) ml/s to (18.0±4.5) ml/s.The mean residual urine volume of 22 cases were reduced from 95 ml (40-250 ml) to 10 ml (0-60 ml).Conclusions Skilled TURP technology for large benign prostatic hyperplasia is a safe treatment with good effect and low complications rates.

Objective To evaluate the efficacy of splenectomy plus selective pericardial devascularization under endoscope in the treatment of advanced schistosomiasis patients with portal hypertension and hypersplenism so as to explore the minimally in-vasive and safer surgical treatment. Methods A secure splenectomy was performed with laparoscope and its supporting devices, and at the same time,the ligation of the left gastric vein and the ligation of esophageal vein perforating vertically into the esopha-gus were also performed in 14 advanced schistosomiasis patients with portal hypertension and hypersplenism. Results Among the 14 patients,the splenic artery was separated and clipped before the treatment of splenic pedicle. One patient was of conversion to open laparotomy for the splenic vein rupture bleeding in the separation. There was no death. Conclusion The operation of sple-nectomy plus selective pericardial devascularization under endoscope is effective,truly minimally invasive,and safe in the treat-ment of advanced schistosomiasis patients with portal hypertension and hypersplenism.

BACKGROUND:Ligamentum flavum hypertrophy is one of the most important factors of lumbar spinal stenosis, but the molecular mechanism is stil not very clear. OBJECTIVE:To explore the role of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 in hypertrophy of the lumbar ligamentum flavum. METHODS: The ligamentum flavum samples were divided into three groups according to different diseases: control group (acquired from the patients with lumbar spinal canal tumor,n=6), lumbar disc herniation (LDH) group (acquired from the patients with LDH,n=6) and lumbar spinal stenosis (LSS) group (acquired from the patients with LSS,n=6). Then the mRNA expressions of basic fibroblast growth factor, connective tissue growth factor, transforming growth factor β1 and colagen I, III, V of the ligamentum flavum were detected using real-time quantitative RT-PCR method. The roles of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 were explored. RESULTS AND CONCLUSION:The expression of basic fibroblast growth factor mRNA in the LSS group was significantly higher than that in the LDH and control groups (bothP < 0.05); the expression of connective tissue growth factor mRNA was not found statisticaly different among the three groups, although it was slightly higher in the LSS group (P> 0.05); the expression of transforming growth factor β1 mRNA was significantly higher in the LSS group than in the LDH and control groups (bothP < 0.01). The colagen I mRNA expressed significantly higher in the LSS group than the LDH and control groups (bothP < 0.05), but both the colagen III and V mRNA showed no significant difference among the three groups (P> 0.05). This study indicate that both basic fibroblast growth factor and transforming growth factor β1 play important roles in the formation process of the lumbar ligamentum flavum hypertrophy, and the main type of the colagen in the hypertrophied ligamentum flavum is colagen I.

With the research on tumor immunity and tumor immunosuppression development,immunotherapies for lung cancer has become a research hotspot.Tumor immune microenvironment plays an important role in tumor genesis,development and metastasis of lung cancer.Tumor-associated macrophages (TAMs) are the dominant leukocyte population found in immune microenvironment of lung cancer.The relationship between TAMs and lung cancer becomes clear with further research.TAMs may become the new target of lung cancer immunotherapy.

Objective To observe the effects of Radix Angelica Sinensis ( RAS) on depression be-havior and cytokines,TNF-αand IL-6,in the hippocampus and prefrontal cortex of depression model rats in-duced by chronic unpredictable mild stress ( CUMS) .Methods 32 adult male rats weighting 140-160 g were randomly divided into 4 groups: Control,CUMS,CUMS+fluoxetine and CUMS+RAS groups.CUMS procedure went on 5 consecutive weeks and during the last 3 weeks the rats in CUMS+fluoxetine and CUMS+RAS groups were taken RAS or fluoxetine via intragastric administration.After 5-week CUMS procedure, rats were subjected to sucrose preference test,forced swimming test and open field test.After behavioral tests were finished,all rats were anesthetized with 10%chloral hydrate (350 mg/kg,intraperitoneal injection) and then decapitated.The hippocampus and prefrontal cortex of rats were separated and ELISA was used to detect the expression of TNF-αand IL-6 in these regions.Results Comparing with control rats, rats exposed to CUMS showed decreased sucrose preference ratio((42±15)%),prolonged immobility time ((68.28±16.50) s),decreased crossing numbers (31.25±21.56) and increased TNF-α((206.14±30.53)pg/ml) and IL-6 ((369.51±103.81)pg/ml) expression in hippocampus and TNF-α((199.33±25.67)pg/ml) and IL-6 ((347.74±81.04) pg/ml) expression in prefrontal cortex ( P<0.01).However,RAS treated rats reversed the behavioral changes such as sucrose preference ratio((66±21)%),immobility time ((32.53±10.26)s) and crossing numbers ( 83.00 ±23.25 ) , meanwhile reduced TNF-α( ( 53.42 ±12.43 ) pg/ml ) and IL-6 ((93.84±13.19)pg/ml) expression in hippocampus,TNF-α((57.58±8.33)pg/ml) and IL-6((91.18± 17.37) pg/ml) expression in prefrontal cortex among stress rats ( P<0.01) .Conclusion RAS can amelio-rate CUMS induced depression behaviors of rats through regulating hippocampus and prefrontal cortex cyto-kines ( TNF-α,IL-6) .