Objective:To evaluate the feasibility of animal model of bilirubin encephalopathy by injecting bilirubin solution into cerebellomedullary cistern of neonatal full-term and premature mice.Methods:Thirty 7-day-old mice were randomly divided into 5 groups:control group,model group 1,model group2,model group3,and model group4.Bilirubin solution was injected into the cerebellomedullary cistern in model groups(bilirubin 20?g/g bodyweight),while equal volume of normal saline was injected into the cerebellomedullary cistern in control group.The mice in model groups were sacrificed at the time of 12h,24h,48 and 72h after injection,respectively,and those in control group were sacrificed at 24h after injection.The apoptosis and necrosis of neurocytes,the expressions of Bcl-2 and Bax proteins were detected by HE stain,immunohistochemical method and TUNEL in the hippocampus,respectively.Preterm mice were delivered by cesarean section at 21 days gestation and fed by surrogate mother mouse until 7 days old.Bilirubin solution was injected into their cerebellomedullary cistern.The mice's abnormal behaviors were observed.Results:The mice of model groups and preterm group appeared abnormal behaviors in different degree.Under light microscope,the neurocytes in their hippocampus appear apoptosis and necrosis.The nissl body in pyramidal cell body and dendron decreased,even dissolved to disappear.TUNEL showed that the apoptosis rate in the hippocampus increased obviously at 12h post-injection,reached the peak at 24h post-injection.The positive rate of Bax protein in the hippocampus reached the peak at 24h post-injection,while the positive rate of Bcl-2 protein decreased to the lowest.Conclsions:The method that injecting bilirubin solution into neonatal mice' cerebellomedullary cistern to set up the animal model of bilirubin encephalopathy is simple and feasible.The peak time of neurocytes' apoptosis in hippocampus was 24h post-injection.It's crucial to prevent bilirubin encephalopathy as early as possible.

Objective To investigate the clinical efficacy of ICARE rebounder tonometer (RBT) by comparing the measurement results of ICARE RBT,applanation tonometer (AT) and non-contact tonometer (NCT).Methods This study included 100 glaucoma suspect cases (100 eyes) with their day and night intraocular pressure (IOP) measurement in Beijing Tongren Hospital from May to November,2014.IOP of each eye was measured by two experienced examiners using RBT,AT and NCT for three times respectively and the mean values were recorded as the final result.Results IOP was measured by RBT,AT,NCT,the values were (19.1s5.7),(18.9±5.0),(15.2±5.0) mmHg (1 mmHg=0.133 kPa),respectively.The difference between RBT and AT was (0.2±1.8) mmHg,and the difference between NCT and AT was-(3.8±2.6) mmHg.There was no significant difference in the values between RBT and AT,t=-1.136,P＞0.05.There was significant difference in the values between NCT and AT,t=14.714,P＜0.01.The results measured by RBT,NCT and AT had correlation,r=0.948,0.822;P＜0.01.The relevant value was 0.973 in two examiners with RBT,which consisted with the result with AT.Conclusion RBT meets the clinical needs and can be widely used in clinical work.

Recently,paclitaxel has been used as a new agent on patients with advanced esophageal cancer,and several studies have demonstrated that paclitaxel and paclitaxel-based regimens have significant activity in patients with advanced esophageal cancer and showed highly response rates than the combination of 5-fluorouracil (5-Fu) and cisplatin.However,there have been some problems which need to be solved such as drug-resistance,side effect,and so forth.The new medicament form of paclitaxel can not only depress poison without anti-allergy but also raise medicament curative effect as well as the pharmacal concentration.Although most of the research on the new medicament form of paclitaxel are still limited in the stage of laboratory,it has possessed potential advance in the treatment of advanced esophageal cancer.

Objective:To study the clinical value of serumsoluhle CD8 antigen(sCD8)inpatients withGraves disease(GD).Methods:The level of serum sCD8 was detected by ELISA in40 healthy controls and 46 GD Patients.Serial measurement were carried out in 28 patientsbefore and after trea treatment with PTU. Results: Serum sCD8 levels were increased signifiantly in untreated GD patients as compared with conrols(21.91 ±9.13 kU/Lvs9.84±3.08kU/L, P ＜0.001).After treatment with PTU for 2 to 7 months,sCD8 levels tetumed to normal when eu-thyroidism was esta established in 22 patients(9.67 ± 5. 14 kU/L) but remained high in 6 unmiproved patients(38.74 ± 9.89 kU/L). No statisticalrelationship was found between sCD8 and T3、T4、TSH、TGA、 TMA.Conchusion: The level of serum sCD8 correlate well with disease activityand may be of value in disease prognosis. PTU show some suppressor effect on immune.

Objective: To discuss the risk factors, subtypes and prognosis of cerebral infarction in hypertensive and non-hypertensive patients. Methods: A total of 976 patients with cerebral infarction were divided into hypertension (n=516) and non-hypertension (n=460) according to their history of hypertension. Single and multiple factor analysis in the risk factors, subtypes of cerebral infarct, and prognosis were performed. Results: The incidence of previous cerebral infarction, lacunar infarction, and posterior circulation infarction in the hypertension group was significantly higher than those in the non-hypertension group. Advanced age, cardiovascular diseases, and diabetes mellitus in the non-hypertension group were significantly more frequent than those in the hypertension group, and there were significant difference. Conclusions: Hypertension, diabetes mellitus, cardiovascular diseases, and advanced age are the main risk factors of cerebral infarction. Hypertension is the major causes of lacunar infarction and posterior circulation infarction.

Objective: To further understand the roles of gap junctional intercellular communication in carcinogenesis and progression of human lung cancer. Methods: The heterologous communication was characterized among human lung epithelial cells HLEC, human lung fibroblasts HLF, human lung giant carcinoma PG cells and its Cx43 transfectants PG/C4 by using a preloading assay. Cx43 immunofluorescent staining and Northern blot were performed to examine Cx43 gene expression. Results: Although both human lung firoblasts HLF and epithelial cells HLEC expressed Cx43 and had very strong homologous communication, HLEC cells were unable to communicate with HLF cells. The human lung carcinoma cell line PG was defective of both homologous communication, and heterologous communication with its epithelial origin HLEC. Transfection of Cx43 into PG cells, which rescued PG cells' homologous communication and enhanced heterologous communication with HLF cells, could restore heterologous coupling with HLEC cells. Conclusion: Selective heterologous communication exists among different kinds of human lung cells. when human lung carcinoma cells lost coupling with its epithelial origin, Cx43 gene expression is not enough to establish heterologous communication between them.

China ; Occupational Diseases ; prevention & control ; Occupational Health ; legislation & jurisprudence

Objective To explore the clinical guiding value of spiral computed tomography(SCT)with three-dimensional computed tomography reconstruction(3-DCT)in percutaneous kyphoplasty(PKP).Methods 24 patients prepared to be treated with PKP were examined under 3-DCT before the treatment.Results According to the contra-indications shown by 3-DCT,11 patients' operation were cancelled;the others were treated with PKP and got good outcomes due to the accurate data provided by 3-DCT.Conclusion SCT plays an important role in PKP.

Objective To investigate the influence of surface grafting density of polyethylene glycol (PEG) molecules on the interactions of nanoparticle with the biomembrane.Methods The dynamics of polymer as well as its shielding effect on the wrap of the membrane were investigated using coarse-grained (CG) molecular dynamics simulations.A coarse grained model force field,MARTINI,was employed for its ability to reproduce experimental properties of various lipid dynamics and some kinds of polymer as well.Results As a result,structural variations and energy changes of grafted PEG molecules on the nanoparticles,adsorbing dynamics of PEGs on the membrane as well as the wrap rates of the membrane were obtained to shed light on the PEGylation-mediated shielding mechanism in the adsorption of PEGylated nanoparticles on the membrane.Conclusions The surface grafting density of PEGylated nanoparicles has been found to play a crucial role in the stealth shielding behavior of PEGylated nanocarriers.These studies are consistent with experimental observations and to some extent give a molecular level interpretation of the macroscale observations of prolonged circulation half-life of stealth nanocarriers.

Objective To analyze the epidemic characteristics of natural foci of plague in Qinghai province in the past twenty years and to provide references for prevention and control of the disease. Methods Excel software was used to analyze the database of human plague cases and the surveillance and survey data of natural foci of plague in Qinghai province from 1999 to 2009. Results Animal plague occurred in Qaidam Basin, Qinghai Plateau and central and southern Plateau year after year, which became key areas of animal plague in Qinghai province. There were many human plague cases every year except 1999, 2000, 2002, 2007, 2008 from 1990 to 2009. Cumulative incidence of human plague was 27 cases, with incidence of 73 cases, 29 cases of death, and death rate 39.73%. Epidemic areas mainly distributed in the 4 states of Yushu, Hainan, Hercynian, Haibei.Prevalent season was from May to October. The most prevalent was pneumonic type among clinical types [60.27%(44/73)]. Conclusions Animal plague has occurred actively in key areas of Qinghai province in recent years,and an outbreak of human plague in some areas is found. Monitoring and early warning should be strengthened in the key areas, and comprehensive prevention and control measures should be implemented to reduce the incidence and prevalence of the plague.