Regeneration ; Tissue Engineering ; Vocal Cords

In new era,field medical equipment gives full scope to the development of medical equipment and fulfillment of daily operation.Meanwhile,to meet the requirements of military reform,basic sanitation service needs to grasp characteristics of medical equipment and to improve management capability of medical equipment.

Objective: To observe the therapeutic effect of nicorandil on microvascular angina pectoris (MAP). Methods: A total of 60 MAP patients were randomly divided into nicorandil group (n=30) and routine treatment group (n=30), and they were treated for 12 weeks. Onset times and duration of angina pectoris, changes of related parameters of ECG treadmill exercise test before and after treatment, and total effective rate were compared between two groups. Results: Compared with before treatment, there were significant reductions in onset times and duration of angina pectoris and maximum depression extent of ST segment (P<0.05 or <0.01) and significant increase in total exercise time (P<0.01) after treatment in both groups; compared with routine treatment group, there were significant reductions in onset times of angina pectoris [(10.3±1.6) times/week vs. (9.6±1.7) times/week] and maximum depression of ST segment [(0.8±0.3)mm vs. (0.6±0.2)mm], and significant increase in total exercise time [(7.8±1.4) min vs. (9.4±1.6) min] and total effective rate (73.3% vs. 93.3%) in nicorandil group, P<0.05 or <0.01. Conclusion: Nicorandil possesses significant therapeutic effect on microvascular angina pectoris, and it is worth further study.

Objective To investigate the effects of avastin combined with As2 O3 on rabbits corneal neovas-cularization (CNV)induced by alkali burn.Methods A total of 48 Japanese rabbits were induced the CNV models by middle alkali burns.The rabbits were randomly divided into four groups.A group was subconjunctivally injected 0.5mL 1mg/mL of As2 O3 and 0.5mL normal sodium,B group was subconjunctivally injected 0.5mL 25mg/mL of avastin and 0.5mL normal sodium,C group was subconjunctivally injected 0.5mL 1mg/mL of As2 O3 and 0.5mL 25mg/mL of avastin,D group was subconjunctivally injected 1.0mL normal sodium,after corneal alkali burn,respec-tively.CNV area at 6 days,12 days,15 days,21 days and 28 days after burns were calculated,the expression of IL -1β,VCAM-1 and VEGF in corneal tissue at 7 days,14 days,28 days after burns were detected by immunohisto-chemical staining.Results Alkali burn after 28 d,the CNV,IL -1β,VCAM-1,VEGF expression rates of A group were (50.28 ±30.26)mm2 ,(6.83 ±1.74)%,(1.52 ±0.91)%,(5.02 ±0.82)%,respectively,which of B group were (55.19 ±20.03)mm2 ,(6.88 ±1.94)%,(1.41 ±0.94)%,(2.91 ±0.84)%,respectively,which of C group were (10.03 ±5.98)mm2 ,(1.96 ±0.99)%,(0.38 ±0.57)%,(1.08 ±0.77)%,respectively,which of D group were (83.18 ±33.05)mm2 ,(10.16 ±2.17)%,(3.74 ±0.83)%,(7.69 ±1.33)%,respectively.The differences among four groups were statistically significant (F =25.33,3.64,13.13,7.23,all P <0.05 ).Compared with D group,the CNV area and the expressions of IL -1β,VCAM-1 and VEGF were significantly decreased in A,B,C group(qCNV =40.294,34.281,89.590,qIL -1β =4.078,4.017,10.043,qVCAM-1 =2.719,2.854,5.791,qVEGF =3.270,8.238,11.392,all P <0.05);Compared with C group,the CNV area and the expressions of IL -1β,VCAM-1 and VEGF were significantly decreased in A,B group (qCNV =49.296,55.309;qIL -1β =5.965,6.026;qVCAM-1 =1.965,1.775;qVEGF =6.790,22.413,all P <0.05);Compared with B group,the CNV area and the expressions of IL -1βand VCAM-1 were not significantly different in A group (P >0.05);Compared with B group,the expression of VEGF was significantly increased in A group (q =4.03,P <0.05).Conclusion Avastin combined with As2 O3 can inhibit the growth of CNV induced by alkali burning in rabbits effectively,and reduce the expressions of IL -1β, VCAM-1 and VEGF in CNV.It has better efficacy than single drug,and it opens up a new way for the prevention and treatment of CNV.

Objective:To observe the change of blood endotoxin level after external biliary drainage with bile extracorporeal bypass in patients with malignant obstructive jaundice.Methods:The external biliary drainages with bile extracorporeal bypass were undergone in 14 patients with malignant obstructive jaundice,peripheral blood endotoxin levels were measured in perioperative period, other 15 cases internal biliary drainage and 20 cases external biliary drainage as contrast.Results:The changes of endotoxin level were not significant in external biliary drainage group,however,the endotoxin level was decreased signficantly in internal biliary drainage or external biliary drainage with bile extracorporeal bypass at postoperation.Conclusion:The external biliary drainage with bile extracorporeal bypass can decrease postoperative blood endotoxin level in patients with malignant obstructive jaundice.

Objective To explore the effect of c-Jun NH2-terminal kinase(JNK)signal transduction pathway on hyperoxia-induced lung injury in rats.Methods Twenty-four Wistar rats aged 3 weeks were randomly divided into 3 groups(n=8):room-air control group,7 d hyperoxia exposure group,and 7 d hyperoxia exposure with inhibitor of JNK intervention group.The rats in hyperoxia exposure group were exposed to high concentration of oxygen [fractional concentration of inspired oxygen(FiO2)≥950 mL?L-1] at normal pressure.The rats in room-air control group were placed in room air(FiO2=210 mL?L-1)at normal pressure.The rats in JNK inhibitor intervention group were intraperitoneally injected 30 mg?kg-1 SP600125 and exposed to hyperoxia 2 h later.The histopathological changes of lung tissues were observed by means of light microscope,therefore the changes of lung W/D weight ratio,total protein in bronchoalveolar lavage fluid(BALF)and lung permeation index were detected.The extent of lung cells apoptosis was analyzed by terminal deoxynucleotidyltrans-ferase-mediated dUTP nick end labeling assay.The protein level of p-JNK was measured by Western blotting analysis.Results Compared with room-air control group,conspicuous hyperaemia,edema,hemorrhage and extensive inflammation cells infiltration in the lung tissues were significantly observed in 7 d hyperoxia exposure group.The lung W/D weight ratio,total protein in BALF,lung permeation index,cell apoptotic index and the p-JNK protein levels of lung tissues all significantly increased in 7 d hyperoxia exposure group compared with those in room-air control group(Pa

Many countries have developed standard strategy under the tendency of the economic globalization.TCM international standardization faces more and more competitions.This article analyzes the competitors and competitive status of TCM international standardization by the research methods of competitive intelligence,competitor analysis and SWOT analysis,and puts forward corresponding strategic measures in details.

Great progress has been made in the study of fatty liver with integrated traditional Chinese and western medicine in aspects of diagnosis, treatment and experimental study, etc. Most researches were designed to utilize diagnostic or model replicating method of western medicine to observe the effects or investigate the action mechanism of compound recipe, single Chinese herb or effective ingredients of Chinese herbs on fatty liver. According to the pathological mechanism of traditional Chinese medicine (TCM), fatty liver is characterized by deficiency in nature and repletion in appearance, which involves three Zang viscera such as liver, spleen and kidney and manifests as spleen Qi deficiency, liver and kidney deficiency, phlegm and dampness heaping internally, and Qi stagnation and blood stasis. This facilitates us to use specific recipe or modified recipe to treat fatty liver from the points of integrated traditional Chinese and western medicine and combining syndrome differentiation with disease differentiation. With gratifying achievement, this kind of approach has been the mainstream of the research on fatty liver and many researchers have reached an agreement on this point domestically. Spleen Fortifying and Blood Invigorating Recipe (SFBVER in brief, invented by our institute) can significantly improve the B ultrasound outcome of the liver in patients with fatty liver, with significant difference in B ultrasound scoring between pre-and post-treatment. It can alleviate the patients' symptoms, improve or regain liver function, decrease waist/buttocks ratio and the content of triglyceride and cholesterol in blood. SFBVER is superior to Dongbao Gantai Recipe in general effective rate. Experimental study also reveals that SFBVER can alleviate CCl(4) induced liver cell fatty degeneration and the inflammatory cell infiltration in rats, decrease the activities of ALT and AST, lower the content of triglyceride in liver, recover SOD activity in liver to normal level. The overall efficacy of SFBVER is superior to that of Dongbao Gantai Recipe. Further correlated study should be focused on inventing new preparation of traditional Chinese medicine and investigating its action mechanism with the guiding of the theory of TCM and referring to the latest discovery in fatty liver research in modern medicine.

The molecular weight of matrix metalloproteinase (MMP)-9 is the largest in MMP family which comes mainly from macrophage and neutrophil.MMP-9 is considered to be involved in the occurrence and metastasis of colorectal cancer.It also provides a new way for colorectal cancer in clinical prevention and treatment.